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The map label for this gene is bioD [H]

Identifier: 162147706

GI number: 162147706

Start: 1956351

End: 1957775

Strand: Direct

Name: bioD [H]

Synonym: GDI_1922

Alternate gene names: 162147706

Gene position: 1956351-1957775 (Clockwise)

Preceding gene: 162147705

Following gene: 162147709

Centisome position: 49.6

GC content: 70.53

Gene sequence:

>1425_bases
ATGAATCCGCGCAAGCACCAGATCGCGGCCCGTTTCGGCGGTGCCGACTCCTATGACGCCGCCGCCCGCATCCAGGCGTC
GGTGGCAGCGCGCCTGGCCGATCGGATCAGGGCCGCCTGCGATGCGGCCGGTGGCCTGGCGCCGGCCCGTATCCTCGAAC
TGGGTTGCGGCACCGGTTTCCTGAGCGCCCATCTGCGCCGCCTGTTCCCCGATGCCATCCTGACGGTTACCGATCTGGCG
CCGGAAATGGTCGAACGCGCGCGCGCGCGCCTGACGCCCCTGGGCGGGGACGTCCGCTATGCGGTCGTGGACGCGGAAGA
TCCCGCATCGGTCGGCACGGGGTTCGACCTGATCTGTTCCAGCCTGTCGATGCAATGGTTCACGGACCCCGCCGCAACGC
TGGACCGCCTGGCCGCGCGGCTGGCGCCGGGCGGCATGATGGCGCTTTCCACCCTCTGCGCCGGCAGCTTCGCGGAATGG
CGTGCGGTGCACCGGGCGGCGGGACTGGACTGCCCGGTGCCGTCCTATCCGGGCCTGGAGCAACTGCAGGCCGACTGGCC
CGGTGCCGGGGTGGGGTCATGGCGGCACGAAACGCTGATCGACCATCCCGCATCCGCGCTGGATTTCGTGCGCGAACTGC
GCCAGATCGGCGCATCCCTGCCGCGCGACGGCGCACGGCCCGCGGGGCCGGGCACGATGCGCCGGCTGTTGCGCGATCTG
GACAACCGGGGCGGCGTTACCGCGACCTACGAGATCGGATACGGCCTGTTTCGCACGCCGTCGCGCCGGGGCGTGTTCGT
GACCGGTACCGATACCGAGGTCGGCAAGACACTGGCGGCGGCGTGCCTGGTCCAGGCCTGGAATGCGTCCTACTGGAAGC
CGCTACAGACCGGCATTGCCGAGGACACCGCCGACAGCGTCACCATTGCCGCGCTGTGCGGCGTGGACCCGGCCCGGATC
GCCCGGCCCGCCGTGGTCCTTCCGGCCCCGCTGTCACCCTTCGACGCGGCGGAGCAGGACGGGATCACGCTCGATGCCGC
GCGGATCGTACTCCCGCCCGCCCGGGACGCGCGTCCCGTGGTGGTCGAAGGGGCAGGTGGGGTCATGGTCCCGATTACGC
GCGACTGCATGATGATCGACCTGATGGTCCGTTTCGCCCTGCCGGTGGTGGTGGTGGCCCGCAGCCAGCTCGGTACGATC
AACCATACGCTGCTGACCCTGCAGGCCCTGCGGTCACGCGGCCTGGCCGTCGCCGGCGTGATCCTGAACGGACAGCCGTC
GCGCGAGGCACGGCGGGCGATCACCCTGTTCGGCGGGGCCCGCGTCCTAGCGGAATTTCCGCATCTGGATCAGGTCGGTC
CCGAACAGGTCGCGAACCTGGCCGGCTTGCTGCCGTCCTATGACGATCTGTGGTGCTATCCCTGA

Upstream 100 bases:

>100_bases
GGCTTGCCGTCGACTGGATCGCGGATGGCGGCCATCTGCTGCCGCTGACCCATCCCGACGCCTGCGCCGCCGCGATTACG
CATATGATGGATGCCGCCCC

Downstream 100 bases:

>100_bases
AACCCGTCCGTCCCGGTCAATACCGGCGGAGCAGGCCGACGAGCTGGCCCTGGATTCGGACCCGGTCCGACGGGACAATG
CGCGGCTCGTAGCGCGCGTT

Product: biotin synthesis protein

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MNPRKHQIAARFGGADSYDAAARIQASVAARLADRIRAACDAAGGLAPARILELGCGTGFLSAHLRRLFPDAILTVTDLA
PEMVERARARLTPLGGDVRYAVVDAEDPASVGTGFDLICSSLSMQWFTDPAATLDRLAARLAPGGMMALSTLCAGSFAEW
RAVHRAAGLDCPVPSYPGLEQLQADWPGAGVGSWRHETLIDHPASALDFVRELRQIGASLPRDGARPAGPGTMRRLLRDL
DNRGGVTATYEIGYGLFRTPSRRGVFVTGTDTEVGKTLAAACLVQAWNASYWKPLQTGIAEDTADSVTIAALCGVDPARI
ARPAVVLPAPLSPFDAAEQDGITLDAARIVLPPARDARPVVVEGAGGVMVPITRDCMMIDLMVRFALPVVVVARSQLGTI
NHTLLTLQALRSRGLAVAGVILNGQPSREARRAITLFGGARVLAEFPHLDQVGPEQVANLAGLLPSYDDLWCYP

Sequences:

>Translated_474_residues
MNPRKHQIAARFGGADSYDAAARIQASVAARLADRIRAACDAAGGLAPARILELGCGTGFLSAHLRRLFPDAILTVTDLA
PEMVERARARLTPLGGDVRYAVVDAEDPASVGTGFDLICSSLSMQWFTDPAATLDRLAARLAPGGMMALSTLCAGSFAEW
RAVHRAAGLDCPVPSYPGLEQLQADWPGAGVGSWRHETLIDHPASALDFVRELRQIGASLPRDGARPAGPGTMRRLLRDL
DNRGGVTATYEIGYGLFRTPSRRGVFVTGTDTEVGKTLAAACLVQAWNASYWKPLQTGIAEDTADSVTIAALCGVDPARI
ARPAVVLPAPLSPFDAAEQDGITLDAARIVLPPARDARPVVVEGAGGVMVPITRDCMMIDLMVRFALPVVVVARSQLGTI
NHTLLTLQALRSRGLAVAGVILNGQPSREARRAITLFGGARVLAEFPHLDQVGPEQVANLAGLLPSYDDLWCYP
>Mature_474_residues
MNPRKHQIAARFGGADSYDAAARIQASVAARLADRIRAACDAAGGLAPARILELGCGTGFLSAHLRRLFPDAILTVTDLA
PEMVERARARLTPLGGDVRYAVVDAEDPASVGTGFDLICSSLSMQWFTDPAATLDRLAARLAPGGMMALSTLCAGSFAEW
RAVHRAAGLDCPVPSYPGLEQLQADWPGAGVGSWRHETLIDHPASALDFVRELRQIGASLPRDGARPAGPGTMRRLLRDL
DNRGGVTATYEIGYGLFRTPSRRGVFVTGTDTEVGKTLAAACLVQAWNASYWKPLQTGIAEDTADSVTIAALCGVDPARI
ARPAVVLPAPLSPFDAAEQDGITLDAARIVLPPARDARPVVVEGAGGVMVPITRDCMMIDLMVRFALPVVVVARSQLGTI
NHTLLTLQALRSRGLAVAGVILNGQPSREARRAITLFGGARVLAEFPHLDQVGPEQVANLAGLLPSYDDLWCYP

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=220, Percent_Identity=30.9090909090909, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324385, Length=190, Percent_Identity=45.7894736842105, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472 [H]

Pfam domain/function: NA

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 50217; Mature: 50217

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRKHQIAARFGGADSYDAAARIQASVAARLADRIRAACDAAGGLAPARILELGCGTGF
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHH
LSAHLRRLFPDAILTVTDLAPEMVERARARLTPLGGDVRYAVVDAEDPASVGTGFDLICS
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHH
SLSMQWFTDPAATLDRLAARLAPGGMMALSTLCAGSFAEWRAVHRAAGLDCPVPSYPGLE
HCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHH
QLQADWPGAGVGSWRHETLIDHPASALDFVRELRQIGASLPRDGARPAGPGTMRRLLRDL
HHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DNRGGVTATYEIGYGLFRTPSRRGVFVTGTDTEVGKTLAAACLVQAWNASYWKPLQTGIA
CCCCCEEEEEEECCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCC
EDTADSVTIAALCGVDPARIARPAVVLPAPLSPFDAAEQDGITLDAARIVLPPARDARPV
CCCCCCEEEEHHHCCCHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEE
VVEGAGGVMVPITRDCMMIDLMVRFALPVVVVARSQLGTINHTLLTLQALRSRGLAVAGV
EEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
ILNGQPSREARRAITLFGGARVLAEFPHLDQVGPEQVANLAGLLPSYDDLWCYP
EECCCCCHHHHHEEHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNPRKHQIAARFGGADSYDAAARIQASVAARLADRIRAACDAAGGLAPARILELGCGTGF
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHH
LSAHLRRLFPDAILTVTDLAPEMVERARARLTPLGGDVRYAVVDAEDPASVGTGFDLICS
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHH
SLSMQWFTDPAATLDRLAARLAPGGMMALSTLCAGSFAEWRAVHRAAGLDCPVPSYPGLE
HCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHH
QLQADWPGAGVGSWRHETLIDHPASALDFVRELRQIGASLPRDGARPAGPGTMRRLLRDL
HHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
DNRGGVTATYEIGYGLFRTPSRRGVFVTGTDTEVGKTLAAACLVQAWNASYWKPLQTGIA
CCCCCEEEEEEECCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCC
EDTADSVTIAALCGVDPARIARPAVVLPAPLSPFDAAEQDGITLDAARIVLPPARDARPV
CCCCCCEEEEHHHCCCHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEE
VVEGAGGVMVPITRDCMMIDLMVRFALPVVVVARSQLGTINHTLLTLQALRSRGLAVAGV
EEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEE
ILNGQPSREARRAITLFGGARVLAEFPHLDQVGPEQVANLAGLLPSYDDLWCYP
EECCCCCHHHHHEEHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA