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The map label for this gene is ycdT [H]

Identifier: 162147651

GI number: 162147651

Start: 1905006

End: 1906814

Strand: Reverse

Name: ycdT [H]

Synonym: GDI_1867

Alternate gene names: 162147651

Gene position: 1906814-1905006 (Counterclockwise)

Preceding gene: 162147653

Following gene: 162147650

Centisome position: 48.35

GC content: 65.73

Gene sequence:

>1809_bases
ATGACGGGCGTCTTTTTTATCAGGAACACACTATCGACCCTGCTGTTCTGCGTCATCTGGAGTATCTGCGCGCTCGCCAG
CGTCACCTGGACCCGTCACGGCGGAAGCGTCTCGATGATCTGGCCGGCCAATGCGATCCTGGTCATGGCCATCCTGCGCA
TGCCCGCCTTCGACCACCGGATCTATCCCCCGCTGCTGTTTGTGCTGTCCAGCCTGGTCAATCTGCTGGATGGCCGGGGA
TGGACCACCAGCGTCGGTTTCAGCGTGGCGAACACGGTCGAGGTCATGATGGCGGCCGTCCTGGTCCACCGGATCAGTCC
GGTGTCGGGCTTCTTCGAACAGGTCGACTGGGCGTTCCGGTTTGCGATCGCGATCTTCGTGTCCGTCGCATGCTCGTCGG
TCATTGCCGGAGGGGCGCTGTTCCTTGGCGAAGGGCGCGACTGGCTGGGGGGAAGCGCCATCTGGATGATGGCGCACCTG
CCCGGCCTGCTGATCACGGTTCCCATGCTGCTGGCCCTCATGCCCGACGGCGAAGGGCGTGAAATCCGGCGCAACGCCCC
CCGGCGATTGACGGTACTGGGCCTGATGGTCCTGGTGGCCTGCATGTCGGTGGTCGTATTCGGGCAGTCGCGCTTTCCGC
TGCAGTTCCTGCTGCTGCCACCCCTGTTGCTGGCGACCTTCCATCTCTACGCCAAGGGGGCCGCGATGTCGGTCGCCATC
ATTACCATGATCGGCAGTTTCTTCATGTTTACCGGCGCGGGGGCCGTTACCATGCTCCACGGGCCGGCCGCACTGAAGGT
CTATGTCTTCCAGCTCGACCTGGCGACGATCTTCTTCTGTGCGTTGCCCCTGGGCGCCGTGCTGACCCAGCGCGACCAGA
AGGCATCGCTGGCCCAGCAGCGGCTGCAGGATTTCCATCATCTGGCCGACCGGGTCGGCGACGTACTGTTCCGCGTCGAC
GACGCGGGATGCTGGAGCTATCTCAACCCGGCCTATGAACAGATGTCCGGCATTTCGGTGGACAAGCGGCTGGGTCAGCC
CATGCTGGACGCCGTCGTGATCGAGGACCGCGCCGCCCTGGCCCAGGCGCAGGCCGAATTGCAGTCCGGCAGCCGCGACG
ACCTGCGCCAGGTCGTCAGCATCTATCGCCGCGACGGAACCGTCCGGCATGTCGAAATCCTGGCCCACCGGCTGGGCGAG
GGCGAAGGGTTTGGCGGCCTGATCCGCGACGTGACGGACCAGTTGACGCTGGACGCGCACAATCGCCAGATCGCCGCCGC
CTATGAACAGGCCGCCAACACCGACGAACTGACCGGCCTGCCCAATCGACGCGCCTTCTTTCAGGTGCTGGACGGGCAGA
TGGCTGCGGCATCCTCGGTGGTCGCGGCGGCGATGCTGGACCTGGACCATTTCAAGTCGATCAACGACAAGTACGGCCAC
CCCATCGGAGACATCGTCCTGCGCCGCGTCGCCACGGTGGTGCGTGCGACCCTGCGGGACACCGACATGGTCGCACGGAT
CGGCGGCGAGGAATTCGCGGTCCTGATCACGAGCGAAAGCCGCGACCGGGCGATCAGCCTGATCCGCCGGGTGGTGCGCG
CGGTATCGGCGGAATCGATCGACCTGCCGACCGGGGTGCGGTTGCAGGTCACGATCAGCGCGGGCATTGCCTGCCGCGAG
GCGGGGGAAGACCGCAACGCCCTGATGTCCCGCGCCGACCAGGCCCTGTATGTCGCCAAACGGACCGGCCGCAACCGGCT
GACCATCGCCGATGCATCCACGGCGGCAACACAGGCCGCGACCCCGTAA

Upstream 100 bases:

>100_bases
CCCGGGTCGCGTCTCGACAGGATATTTCCGATCCTGTCCCTCATTTTTCGGAAATGGTTTTGGTGATATTACGAAAGGAA
GCATGATCGGGTCGTGAAAG

Downstream 100 bases:

>100_bases
GCCACGCGGATCGCGTCGGGGCTGAGGTCTCGACCATCTGAAAAAGGAATCCGGTGGCATGGTCATGACCATGCGCCCCG
ATAAAATCCAAGACACCGTT

Product: GGDEF family signaling protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MTGVFFIRNTLSTLLFCVIWSICALASVTWTRHGGSVSMIWPANAILVMAILRMPAFDHRIYPPLLFVLSSLVNLLDGRG
WTTSVGFSVANTVEVMMAAVLVHRISPVSGFFEQVDWAFRFAIAIFVSVACSSVIAGGALFLGEGRDWLGGSAIWMMAHL
PGLLITVPMLLALMPDGEGREIRRNAPRRLTVLGLMVLVACMSVVVFGQSRFPLQFLLLPPLLLATFHLYAKGAAMSVAI
ITMIGSFFMFTGAGAVTMLHGPAALKVYVFQLDLATIFFCALPLGAVLTQRDQKASLAQQRLQDFHHLADRVGDVLFRVD
DAGCWSYLNPAYEQMSGISVDKRLGQPMLDAVVIEDRAALAQAQAELQSGSRDDLRQVVSIYRRDGTVRHVEILAHRLGE
GEGFGGLIRDVTDQLTLDAHNRQIAAAYEQAANTDELTGLPNRRAFFQVLDGQMAAASSVVAAAMLDLDHFKSINDKYGH
PIGDIVLRRVATVVRATLRDTDMVARIGGEEFAVLITSESRDRAISLIRRVVRAVSAESIDLPTGVRLQVTISAGIACRE
AGEDRNALMSRADQALYVAKRTGRNRLTIADASTAATQAATP

Sequences:

>Translated_602_residues
MTGVFFIRNTLSTLLFCVIWSICALASVTWTRHGGSVSMIWPANAILVMAILRMPAFDHRIYPPLLFVLSSLVNLLDGRG
WTTSVGFSVANTVEVMMAAVLVHRISPVSGFFEQVDWAFRFAIAIFVSVACSSVIAGGALFLGEGRDWLGGSAIWMMAHL
PGLLITVPMLLALMPDGEGREIRRNAPRRLTVLGLMVLVACMSVVVFGQSRFPLQFLLLPPLLLATFHLYAKGAAMSVAI
ITMIGSFFMFTGAGAVTMLHGPAALKVYVFQLDLATIFFCALPLGAVLTQRDQKASLAQQRLQDFHHLADRVGDVLFRVD
DAGCWSYLNPAYEQMSGISVDKRLGQPMLDAVVIEDRAALAQAQAELQSGSRDDLRQVVSIYRRDGTVRHVEILAHRLGE
GEGFGGLIRDVTDQLTLDAHNRQIAAAYEQAANTDELTGLPNRRAFFQVLDGQMAAASSVVAAAMLDLDHFKSINDKYGH
PIGDIVLRRVATVVRATLRDTDMVARIGGEEFAVLITSESRDRAISLIRRVVRAVSAESIDLPTGVRLQVTISAGIACRE
AGEDRNALMSRADQALYVAKRTGRNRLTIADASTAATQAATP
>Mature_601_residues
TGVFFIRNTLSTLLFCVIWSICALASVTWTRHGGSVSMIWPANAILVMAILRMPAFDHRIYPPLLFVLSSLVNLLDGRGW
TTSVGFSVANTVEVMMAAVLVHRISPVSGFFEQVDWAFRFAIAIFVSVACSSVIAGGALFLGEGRDWLGGSAIWMMAHLP
GLLITVPMLLALMPDGEGREIRRNAPRRLTVLGLMVLVACMSVVVFGQSRFPLQFLLLPPLLLATFHLYAKGAAMSVAII
TMIGSFFMFTGAGAVTMLHGPAALKVYVFQLDLATIFFCALPLGAVLTQRDQKASLAQQRLQDFHHLADRVGDVLFRVDD
AGCWSYLNPAYEQMSGISVDKRLGQPMLDAVVIEDRAALAQAQAELQSGSRDDLRQVVSIYRRDGTVRHVEILAHRLGEG
EGFGGLIRDVTDQLTLDAHNRQIAAAYEQAANTDELTGLPNRRAFFQVLDGQMAAASSVVAAAMLDLDHFKSINDKYGHP
IGDIVLRRVATVVRATLRDTDMVARIGGEEFAVLITSESRDRAISLIRRVVRAVSAESIDLPTGVRLQVTISAGIACREA
GEDRNALMSRADQALYVAKRTGRNRLTIADASTAATQAATP

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=39.1566265060241, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1788381, Length=322, Percent_Identity=29.1925465838509, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=215, Percent_Identity=37.2093023255814, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=37.1951219512195, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=33.9506172839506, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1787541, Length=163, Percent_Identity=36.8098159509202, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=33.9506172839506, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=34.0782122905028, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=33.75, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=32.5153374233129, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87081977, Length=138, Percent_Identity=34.0579710144928, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788956, Length=217, Percent_Identity=29.4930875576037, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=157, Percent_Identity=26.7515923566879, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 65430; Mature: 65299

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGVFFIRNTLSTLLFCVIWSICALASVTWTRHGGSVSMIWPANAILVMAILRMPAFDHR
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCHHHHHHHHHHCCCCCCC
IYPPLLFVLSSLVNLLDGRGWTTSVGFSVANTVEVMMAAVLVHRISPVSGFFEQVDWAFR
CCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FAIAIFVSVACSSVIAGGALFLGEGRDWLGGSAIWMMAHLPGLLITVPMLLALMPDGEGR
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
EIRRNAPRRLTVLGLMVLVACMSVVVFGQSRFPLQFLLLPPLLLATFHLYAKGAAMSVAI
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
ITMIGSFFMFTGAGAVTMLHGPAALKVYVFQLDLATIFFCALPLGAVLTQRDQKASLAQQ
HHHHHHHHHHHCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RLQDFHHLADRVGDVLFRVDDAGCWSYLNPAYEQMSGISVDKRLGQPMLDAVVIEDRAAL
HHHHHHHHHHHHHHHEEEECCCCCHHHCCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHH
AQAQAELQSGSRDDLRQVVSIYRRDGTVRHVEILAHRLGEGEGFGGLIRDVTDQLTLDAH
HHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCC
NRQIAAAYEQAANTDELTGLPNRRAFFQVLDGQMAAASSVVAAAMLDLDHFKSINDKYGH
CCHHHHHHHHHCCCCHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHCCC
PIGDIVLRRVATVVRATLRDTDMVARIGGEEFAVLITSESRDRAISLIRRVVRAVSAESI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
DLPTGVRLQVTISAGIACREAGEDRNALMSRADQALYVAKRTGRNRLTIADASTAATQAA
CCCCCCEEEEEEECCEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHCCCCC
TP
CC
>Mature Secondary Structure 
TGVFFIRNTLSTLLFCVIWSICALASVTWTRHGGSVSMIWPANAILVMAILRMPAFDHR
CCEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCHHHHHHHHHHCCCCCCC
IYPPLLFVLSSLVNLLDGRGWTTSVGFSVANTVEVMMAAVLVHRISPVSGFFEQVDWAFR
CCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
FAIAIFVSVACSSVIAGGALFLGEGRDWLGGSAIWMMAHLPGLLITVPMLLALMPDGEGR
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
EIRRNAPRRLTVLGLMVLVACMSVVVFGQSRFPLQFLLLPPLLLATFHLYAKGAAMSVAI
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHH
ITMIGSFFMFTGAGAVTMLHGPAALKVYVFQLDLATIFFCALPLGAVLTQRDQKASLAQQ
HHHHHHHHHHHCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RLQDFHHLADRVGDVLFRVDDAGCWSYLNPAYEQMSGISVDKRLGQPMLDAVVIEDRAAL
HHHHHHHHHHHHHHHEEEECCCCCHHHCCHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHH
AQAQAELQSGSRDDLRQVVSIYRRDGTVRHVEILAHRLGEGEGFGGLIRDVTDQLTLDAH
HHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCC
NRQIAAAYEQAANTDELTGLPNRRAFFQVLDGQMAAASSVVAAAMLDLDHFKSINDKYGH
CCHHHHHHHHHCCCCHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHCCC
PIGDIVLRRVATVVRATLRDTDMVARIGGEEFAVLITSESRDRAISLIRRVVRAVSAESI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
DLPTGVRLQVTISAGIACREAGEDRNALMSRADQALYVAKRTGRNRLTIADASTAATQAA
CCCCCCEEEEEEECCEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHCCCCC
TP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]