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The map label for this gene is xthA [H]

Identifier: 162147600

GI number: 162147600

Start: 1849785

End: 1850597

Strand: Reverse

Name: xthA [H]

Synonym: GDI_1816

Alternate gene names: 162147600

Gene position: 1850597-1849785 (Counterclockwise)

Preceding gene: 162147601

Following gene: 162147598

Centisome position: 46.92

GC content: 65.56

Gene sequence:

>813_bases
GTGATCGGCAATGCCACACATATGCGCATCGTCACGTGGAATATCAATTCGCTTCGGCTGCGGCTGCCGCTGCTGGCCCG
GCTGGGCGCGGAGCTGCGGCCCGACATCATCTGCCTGCAGGAGACCAAGGTCCCCGACGACCTGTTCCCCGCCGATGCGA
TCCGCGACCTGGGCTATGTCCATATCCAGCATCGCGGGATGAAATCCTATAACGGCGTCGCCATCCTGTCGCGCGTTCCG
CTGACCCCCCTGGACGACACGCCGGACTGGTGCGCCCGCAGCGACTGCCGTCACATCGCGGCCTCGTTCCCCATGGGGGG
GCGGCCGGTGGAACTGCATAATTTCTACGTGCCCGCCGGTGGCGACATCCCCGACCCGGATGCCAACCCAAAATTCGCCC
ACAAGCTGGCTTTCGTGGACGAAGCCACGTCCTGGTTCACGGGCCGCACCAGCCACCGCACCATCCTGGTGGGTGACCTG
AACATCGCCCCGCTGGAACAGGATGTGTGGAGCCACAAGCAGCTGCTGAACGTGGTCAGCCACACCCCACCGGAAGTCAC
GCGGCTTATGGCGTGGCAGGCCTGCGGCTTCGTCGATGCGATGCGCCATTTCGTGCCGCCCGACGAGAAGCTGTATACGT
GGTGGTCCTACCGCAACCGCGACTGGAAGGCCTCCAATCGCGGCCGCCGCCTGGACCATGTCTGGATCACGCCCGACCTG
ACGGCGGCGCTGAAGGACATGACGGTGCTGCGCGAGGCACGGGACTGGCCCACGACGTCGGACCACGTGCCCGTCGCGAT
GGATTTCGCCTGA

Upstream 100 bases:

>100_bases
CCCCTCCGCGCGATTTTTTTTGGACCGCCGCATCATCCGGGCAATGCGCCCCTTTGCCCGCCGGTGCGCACGTGGCGGAT
TAGATCTTGTCAGGGGACGC

Downstream 100 bases:

>100_bases
CAGGGCTGCCCGCAAACGTGGGCTGATGAGCGGGAGCGTCCCAAAGGGCCCGCCTCCGCCATGTGTTTCTGCGCACCGGA
GCATCCCGGAAACGCGCGCC

Product: exodeoxyribonuclease III protein

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MIGNATHMRIVTWNINSLRLRLPLLARLGAELRPDIICLQETKVPDDLFPADAIRDLGYVHIQHRGMKSYNGVAILSRVP
LTPLDDTPDWCARSDCRHIAASFPMGGRPVELHNFYVPAGGDIPDPDANPKFAHKLAFVDEATSWFTGRTSHRTILVGDL
NIAPLEQDVWSHKQLLNVVSHTPPEVTRLMAWQACGFVDAMRHFVPPDEKLYTWWSYRNRDWKASNRGRRLDHVWITPDL
TAALKDMTVLREARDWPTTSDHVPVAMDFA

Sequences:

>Translated_270_residues
MIGNATHMRIVTWNINSLRLRLPLLARLGAELRPDIICLQETKVPDDLFPADAIRDLGYVHIQHRGMKSYNGVAILSRVP
LTPLDDTPDWCARSDCRHIAASFPMGGRPVELHNFYVPAGGDIPDPDANPKFAHKLAFVDEATSWFTGRTSHRTILVGDL
NIAPLEQDVWSHKQLLNVVSHTPPEVTRLMAWQACGFVDAMRHFVPPDEKLYTWWSYRNRDWKASNRGRRLDHVWITPDL
TAALKDMTVLREARDWPTTSDHVPVAMDFA
>Mature_270_residues
MIGNATHMRIVTWNINSLRLRLPLLARLGAELRPDIICLQETKVPDDLFPADAIRDLGYVHIQHRGMKSYNGVAILSRVP
LTPLDDTPDWCARSDCRHIAASFPMGGRPVELHNFYVPAGGDIPDPDANPKFAHKLAFVDEATSWFTGRTSHRTILVGDL
NIAPLEQDVWSHKQLLNVVSHTPPEVTRLMAWQACGFVDAMRHFVPPDEKLYTWWSYRNRDWKASNRGRRLDHVWITPDL
TAALKDMTVLREARDWPTTSDHVPVAMDFA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=279, Percent_Identity=27.9569892473118, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI18375503, Length=279, Percent_Identity=27.9569892473118, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI18375501, Length=279, Percent_Identity=27.9569892473118, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=31.1111111111111, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30714; Mature: 30714

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGNATHMRIVTWNINSLRLRLPLLARLGAELRPDIICLQETKVPDDLFPADAIRDLGYV
CCCCCEEEEEEEEECCCEEEEEHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCEE
HIQHRGMKSYNGVAILSRVPLTPLDDTPDWCARSDCRHIAASFPMGGRPVELHNFYVPAG
EEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
GDIPDPDANPKFAHKLAFVDEATSWFTGRTSHRTILVGDLNIAPLEQDVWSHKQLLNVVS
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
HTPPEVTRLMAWQACGFVDAMRHFVPPDEKLYTWWSYRNRDWKASNRGRRLDHVWITPDL
CCCHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCH
TAALKDMTVLREARDWPTTSDHVPVAMDFA
HHHHHHHHHHHHHHCCCCCCCCCCEEEECC
>Mature Secondary Structure
MIGNATHMRIVTWNINSLRLRLPLLARLGAELRPDIICLQETKVPDDLFPADAIRDLGYV
CCCCCEEEEEEEEECCCEEEEEHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCEE
HIQHRGMKSYNGVAILSRVPLTPLDDTPDWCARSDCRHIAASFPMGGRPVELHNFYVPAG
EEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
GDIPDPDANPKFAHKLAFVDEATSWFTGRTSHRTILVGDLNIAPLEQDVWSHKQLLNVVS
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
HTPPEVTRLMAWQACGFVDAMRHFVPPDEKLYTWWSYRNRDWKASNRGRRLDHVWITPDL
CCCHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCH
TAALKDMTVLREARDWPTTSDHVPVAMDFA
HHHHHHHHHHHHHHCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]