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The map label for this gene is lipB [H]

Identifier: 162147586

GI number: 162147586

Start: 1833615

End: 1834403

Strand: Direct

Name: lipB [H]

Synonym: GDI_1802

Alternate gene names: 162147586

Gene position: 1833615-1834403 (Clockwise)

Preceding gene: 162147585

Following gene: 162147587

Centisome position: 46.49

GC content: 69.96

Gene sequence:

>789_bases
ATGGAACTGATCGTCCTGGGATGCGGCGGGTCGGCGGGGGTGCCGATGATCGGCGGCCCCGACGGCGCTGGCGACTGGGG
CGTCTGCGATCCGGCGGAACCCCGCAACCGCCGCACCCGCGCATCCGTCCTGCTGCGGGGCGGGGACGGGGCGGTGCTGC
TGGATACCGGCCCCGATCTGCGTGACCAGTTGCTGGCCCAGCGGATCGACCGGTTCGACGCGATCCTCTACACCCACGCC
CATGCCGATCACATCGCGGGGCTGGACGAGGTCCGCGCCATCAACCGGGTGATCGACCGGCCGCTGCCGGTCTATGGCAC
GCCCCCGGTGCTGACCGACCTGGAAAACCGCTTCAATTACGCGTTTCGCCCCTGGTCCCCGCCCGGATTCTATCGCCCGG
TGGTGGTGCCGCATGTCGTGCATGCCGGCCAGACGCTGGAGGTCGCGGGCCTGCATCTGCGCCTGTTCGAGCAGATACAC
GGCCGCACCCTGTCGCTGGGAGTGCGCTGCGGCACGATCGCCTATTCCACCGACGTGGTCGAACTGCTGGACGAGGCGTT
CGCGGCGCTGGCCGGGATCGAAACCTGGGTGGTGGACTGCTTCCAGCGTTCTGCCGCCCATAGCGCGCATGCGTGGCTGG
ACCGGGTCCTGGAATGGCGGGCCCGGATCGCGCCGCGCCGGGTGATCCTGACCCATATGGGCCCCGACATGGACTGGGCC
TGGATGCGGGCCAACCTGCCGGACGGGGTCGAGGCCGCGTTCGACGGCATGCGCGTCGCCTTTCACTGA

Upstream 100 bases:

>100_bases
GGCGCGCCGGCTGGCGGAAGTACGGGACATGACGCCCGAAAGCCTGGCCGAGGTCACGACCGCGAACTTCCACCGGCTGT
TCAGCCGGGCGGCCTGACCG

Downstream 100 bases:

>100_bases
CCCGGGTCACTGACCCGGGCCGCCGACCCCGGTTTTGCAAAACCCGTGCAGGCTGCCGAGCATTTTGCAGCGACGCGCGA
TTTAGGTTGAATCGCACGCG

Product: beta-lactamase-like

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MELIVLGCGGSAGVPMIGGPDGAGDWGVCDPAEPRNRRTRASVLLRGGDGAVLLDTGPDLRDQLLAQRIDRFDAILYTHA
HADHIAGLDEVRAINRVIDRPLPVYGTPPVLTDLENRFNYAFRPWSPPGFYRPVVVPHVVHAGQTLEVAGLHLRLFEQIH
GRTLSLGVRCGTIAYSTDVVELLDEAFAALAGIETWVVDCFQRSAAHSAHAWLDRVLEWRARIAPRRVILTHMGPDMDWA
WMRANLPDGVEAAFDGMRVAFH

Sequences:

>Translated_262_residues
MELIVLGCGGSAGVPMIGGPDGAGDWGVCDPAEPRNRRTRASVLLRGGDGAVLLDTGPDLRDQLLAQRIDRFDAILYTHA
HADHIAGLDEVRAINRVIDRPLPVYGTPPVLTDLENRFNYAFRPWSPPGFYRPVVVPHVVHAGQTLEVAGLHLRLFEQIH
GRTLSLGVRCGTIAYSTDVVELLDEAFAALAGIETWVVDCFQRSAAHSAHAWLDRVLEWRARIAPRRVILTHMGPDMDWA
WMRANLPDGVEAAFDGMRVAFH
>Mature_262_residues
MELIVLGCGGSAGVPMIGGPDGAGDWGVCDPAEPRNRRTRASVLLRGGDGAVLLDTGPDLRDQLLAQRIDRFDAILYTHA
HADHIAGLDEVRAINRVIDRPLPVYGTPPVLTDLENRFNYAFRPWSPPGFYRPVVVPHVVHAGQTLEVAGLHLRLFEQIH
GRTLSLGVRCGTIAYSTDVVELLDEAFAALAGIETWVVDCFQRSAAHSAHAWLDRVLEWRARIAPRRVILTHMGPDMDWA
WMRANLPDGVEAAFDGMRVAFH

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=234, Percent_Identity=31.1965811965812, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 28847; Mature: 28847

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIVLGCGGSAGVPMIGGPDGAGDWGVCDPAEPRNRRTRASVLLRGGDGAVLLDTGPDL
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCH
RDQLLAQRIDRFDAILYTHAHADHIAGLDEVRAINRVIDRPLPVYGTPPVLTDLENRFNY
HHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
AFRPWSPPGFYRPVVVPHVVHAGQTLEVAGLHLRLFEQIHGRTLSLGVRCGTIAYSTDVV
CCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHCCCEEEEEEEECEEEEHHHHH
ELLDEAFAALAGIETWVVDCFQRSAAHSAHAWLDRVLEWRARIAPRRVILTHMGPDMDWA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEE
WMRANLPDGVEAAFDGMRVAFH
EEECCCCCHHHHHHCCEEEECC
>Mature Secondary Structure
MELIVLGCGGSAGVPMIGGPDGAGDWGVCDPAEPRNRRTRASVLLRGGDGAVLLDTGPDL
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCH
RDQLLAQRIDRFDAILYTHAHADHIAGLDEVRAINRVIDRPLPVYGTPPVLTDLENRFNY
HHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
AFRPWSPPGFYRPVVVPHVVHAGQTLEVAGLHLRLFEQIHGRTLSLGVRCGTIAYSTDVV
CCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHCCCEEEEEEEECEEEEHHHHH
ELLDEAFAALAGIETWVVDCFQRSAAHSAHAWLDRVLEWRARIAPRRVILTHMGPDMDWA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEE
WMRANLPDGVEAAFDGMRVAFH
EEECCCCCHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]