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The map label for this gene is glpF [H]

Identifier: 162147536

GI number: 162147536

Start: 1776337

End: 1777185

Strand: Direct

Name: glpF [H]

Synonym: GDI_1752

Alternate gene names: 162147536

Gene position: 1776337-1777185 (Clockwise)

Preceding gene: 162147535

Following gene: 162147537

Centisome position: 45.04

GC content: 67.61

Gene sequence:

>849_bases
ATGTTCAGGAACAGACAATTCATCGGGGAGCTGATCTCCGAATTCGTCGCGGTCTTCATCATCATCGTGATCGGTGATTC
CGTGGCGGCGATGTACACGCTCTATGACCCCAGCCCGTACAAGACGGCCTATTGGGGGGTCTGCATCGTCTGGGGGCTGG
GGGTCACGATCGCGATCTACGTCACCGGCGCGGTGTCGGGCACGCACGCCAACCCGGCCGTCACGCTGGCGTTGGCGCTC
TATCGGGGCTTTCCCTGGCGCAAGGTCCTGCCCTACAGCGCCGTCCAGGTGCTGGGCGGGATGGCGGGGGCGGCGATCGT
CTACGTGCTGTATGCGCCGGTGATCGATCACTTCAACCAGTTGCATCACCTGGACCGGCTGCATGACGGCGGGGCCGCCG
GGGTGTTCTTCACCCATCCCGGGGATTTCGTGACGCCGCTTCACGCCCTGGTGGACGAGGCCATCCTGACCGCGATCCTG
CTGTTCGGCATCTTCGCGGTGACGTGCGAATATAATATCCAGGCGCCGCAGGCGAATTCCGGCGCGCTGATCATCGGCCT
GCTGGTGGCGGCGCTGGGCGGGTCCGCGGGATATCTGGAGGCGTGGGCCCTGAACCCGGCGCGCGACCTGGGGCCGCGCC
TGTTCTGCTTCCTGGCCGGGTGGGGGCCGTCCGCCCTGCCGTCGCCCGGCGTATTCTGGTGGGTGCCGATCATGGGGCCG
CTGCTGGGCGGCGTGGTCGGGGCAGGGGCGTACCAGATGCTGATCCGGCCCTACATGCCGCGCCGGCAGCCGCCGGTCCT
GGTCGAGACCCTGCCCGCATCCGATGCCGCGATCCGGCGGTCGTCGTGA

Upstream 100 bases:

>100_bases
CGAACCATGCCCGGCCCGGGTCGCCCTGGCCGGCTGACCGCGCCGGCCGAATACCGAAAACCACACATTTCCCCAACAAG
AAACCGAAAAGAAGTGGGAC

Downstream 100 bases:

>100_bases
CGGTCATTCCAAGTCGGGAGACAAGAAAATCATGAACAAGAAGACGACCATCCTGGCGATCGATCAGGGCACGACGTCCA
CCCGCAGCATCATCTTCGAT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MFRNRQFIGELISEFVAVFIIIVIGDSVAAMYTLYDPSPYKTAYWGVCIVWGLGVTIAIYVTGAVSGTHANPAVTLALAL
YRGFPWRKVLPYSAVQVLGGMAGAAIVYVLYAPVIDHFNQLHHLDRLHDGGAAGVFFTHPGDFVTPLHALVDEAILTAIL
LFGIFAVTCEYNIQAPQANSGALIIGLLVAALGGSAGYLEAWALNPARDLGPRLFCFLAGWGPSALPSPGVFWWVPIMGP
LLGGVVGAGAYQMLIRPYMPRRQPPVLVETLPASDAAIRRSS

Sequences:

>Translated_282_residues
MFRNRQFIGELISEFVAVFIIIVIGDSVAAMYTLYDPSPYKTAYWGVCIVWGLGVTIAIYVTGAVSGTHANPAVTLALAL
YRGFPWRKVLPYSAVQVLGGMAGAAIVYVLYAPVIDHFNQLHHLDRLHDGGAAGVFFTHPGDFVTPLHALVDEAILTAIL
LFGIFAVTCEYNIQAPQANSGALIIGLLVAALGGSAGYLEAWALNPARDLGPRLFCFLAGWGPSALPSPGVFWWVPIMGP
LLGGVVGAGAYQMLIRPYMPRRQPPVLVETLPASDAAIRRSS
>Mature_282_residues
MFRNRQFIGELISEFVAVFIIIVIGDSVAAMYTLYDPSPYKTAYWGVCIVWGLGVTIAIYVTGAVSGTHANPAVTLALAL
YRGFPWRKVLPYSAVQVLGGMAGAAIVYVLYAPVIDHFNQLHHLDRLHDGGAAGVFFTHPGDFVTPLHALVDEAILTAIL
LFGIFAVTCEYNIQAPQANSGALIIGLLVAALGGSAGYLEAWALNPARDLGPRLFCFLAGWGPSALPSPGVFWWVPIMGP
LLGGVVGAGAYQMLIRPYMPRRQPPVLVETLPASDAAIRRSS

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4502187, Length=275, Percent_Identity=35.2727272727273, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI157266307, Length=269, Percent_Identity=35.6877323420074, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4826645, Length=277, Percent_Identity=33.5740072202166, Blast_Score=148, Evalue=4e-36,
Organism=Homo sapiens, GI22538420, Length=265, Percent_Identity=33.2075471698113, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI310133356, Length=209, Percent_Identity=34.4497607655502, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI310114181, Length=209, Percent_Identity=34.4497607655502, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4502181, Length=262, Percent_Identity=27.8625954198473, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4755125, Length=262, Percent_Identity=27.8625954198473, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790362, Length=264, Percent_Identity=39.3939393939394, Blast_Score=167, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI71992966, Length=261, Percent_Identity=31.8007662835249, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71992961, Length=258, Percent_Identity=30.2325581395349, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71994009, Length=263, Percent_Identity=31.1787072243346, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17544068, Length=255, Percent_Identity=32.156862745098, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17531429, Length=267, Percent_Identity=28.4644194756554, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17531431, Length=259, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI32564052, Length=272, Percent_Identity=29.7794117647059, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533613, Length=272, Percent_Identity=29.7794117647059, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558372, Length=264, Percent_Identity=28.7878787878788, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=225, Percent_Identity=36, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6322985, Length=212, Percent_Identity=32.5471698113208, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 30182; Mature: 30182

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRNRQFIGELISEFVAVFIIIVIGDSVAAMYTLYDPSPYKTAYWGVCIVWGLGVTIAIY
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHEEEEE
VTGAVSGTHANPAVTLALALYRGFPWRKVLPYSAVQVLGGMAGAAIVYVLYAPVIDHFNQ
EEECCCCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHHLDRLHDGGAAGVFFTHPGDFVTPLHALVDEAILTAILLFGIFAVTCEYNIQAPQANS
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
GALIIGLLVAALGGSAGYLEAWALNPARDLGPRLFCFLAGWGPSALPSPGVFWWVPIMGP
CHHHHHHHHHHHCCCCCCEEEEECCCHHHCCCEEEEEEECCCCCCCCCCCCEEEHHHHHH
LLGGVVGAGAYQMLIRPYMPRRQPPVLVETLPASDAAIRRSS
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHCCCC
>Mature Secondary Structure
MFRNRQFIGELISEFVAVFIIIVIGDSVAAMYTLYDPSPYKTAYWGVCIVWGLGVTIAIY
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHEEEEE
VTGAVSGTHANPAVTLALALYRGFPWRKVLPYSAVQVLGGMAGAAIVYVLYAPVIDHFNQ
EEECCCCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHHLDRLHDGGAAGVFFTHPGDFVTPLHALVDEAILTAILLFGIFAVTCEYNIQAPQANS
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCC
GALIIGLLVAALGGSAGYLEAWALNPARDLGPRLFCFLAGWGPSALPSPGVFWWVPIMGP
CHHHHHHHHHHHCCCCCCEEEEECCCHHHCCCEEEEEEECCCCCCCCCCCCEEEHHHHHH
LLGGVVGAGAYQMLIRPYMPRRQPPVLVETLPASDAAIRRSS
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]