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The map label for this gene is trwC [H]

Identifier: 162147374

GI number: 162147374

Start: 1591194

End: 1592144

Strand: Direct

Name: trwC [H]

Synonym: GDI_1588

Alternate gene names: 162147374

Gene position: 1591194-1592144 (Clockwise)

Preceding gene: 162147373

Following gene: 162147375

Centisome position: 40.34

GC content: 56.36

Gene sequence:

>951_bases
TTGTCGGATGTCGAATATTATATTGAGTCTATCGATGAGGGGCTCGCGGCGCAAACTGCTTTGACGGGAGAGCGGGAACG
TCCCGCACGAGGACATCGCAAGCGGGGGGATTGGTCAGGGCGGGATCGGGCTGCCTATTATGTGGACAATGGCACAGGTG
AAAAACCCGGCATCTGGTGGACGAATGTCCTGAAGGCTCCGGTACAGCCTCTTCCGTTTGTCTGTCAGAGTGCCGAGGTC
AGTCCATCTGATTTCAGGGCGTTGGCGAGGGGATGCGATCCGCTGACGGGTCAGGCTCTGGTTCAGAGCAAAGGGCAACG
TCGTGTTGGGTATGATCTGCAATTTGCAGCCCCCAAATCGGTATCGGTTTTGTGGGCCATGGGAACAGACATTCAGCGTG
CCAGAATTGAATCGGCCCAGGCGAAAGCCGTTTTTGCGGCACTTGATTACGCCCTCAGAACAGGTCTGATCGTCACCCGT
CGAGGTCACAATGGCGGTCAAAGAGAAACGCCCGCTGAGTTAATGGCCGGCACATTTCTGCACACCACCAGTCGTGCGGG
GGATCCCCAGATCCATACGCATGCCGTGTTGATGAATGTGTGTCGCCGTAAGGATGGCTCAACTGGCACAGTGGATAATC
GGGAACTTCTTTTACATCAACAGGAAATGGGTGCCGTCTATCGTCTGGCATTGAGTGAGGCGCTGGAACGAGACATTGGT
CTGACAACACTCAAGGATGGTCGGAATTTCCGGGTGGCCGGTGTGGCGCCCAGTGTGGAAGAGCGCTTCTCAAAAAGGCG
GGCGGCCATTGAACAGGAAGCGCGTAAAAGGGGGATACAGACAGCCCACCATAGGGAAGCGGCCGAACATATTTCCAGGA
ATACGCGCGCAGCCAAGTCAGACCTCCCGTCACGGGCCGTTTTGCGGGCACGGTGGGAACAGGAGTGTTGA

Upstream 100 bases:

>100_bases
ATGAGGCCTGACCGGAACAGAGGATCCCGTTCAGACGGGGCTATCCTGATCAGACAGTTCAGTCAGGTCAGGATACAAAA
GCATGTTTACTTGCACGCCT

Downstream 100 bases:

>100_bases
TTTCCAGCGAGAACTGACCCTGTAGGGGCGGAAATTTTCATTGAGAATTGACCCGTGTCTGACACTTCCCCGGAGCAACT
GTCCGGGGGTTAAAGGAGTG

Product: conjugal transfer protein TrwC

Products: NA

Alternate protein names: DNA helicase I [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSDVEYYIESIDEGLAAQTALTGERERPARGHRKRGDWSGRDRAAYYVDNGTGEKPGIWWTNVLKAPVQPLPFVCQSAEV
SPSDFRALARGCDPLTGQALVQSKGQRRVGYDLQFAAPKSVSVLWAMGTDIQRARIESAQAKAVFAALDYALRTGLIVTR
RGHNGGQRETPAELMAGTFLHTTSRAGDPQIHTHAVLMNVCRRKDGSTGTVDNRELLLHQQEMGAVYRLALSEALERDIG
LTTLKDGRNFRVAGVAPSVEERFSKRRAAIEQEARKRGIQTAHHREAAEHISRNTRAAKSDLPSRAVLRARWEQEC

Sequences:

>Translated_316_residues
MSDVEYYIESIDEGLAAQTALTGERERPARGHRKRGDWSGRDRAAYYVDNGTGEKPGIWWTNVLKAPVQPLPFVCQSAEV
SPSDFRALARGCDPLTGQALVQSKGQRRVGYDLQFAAPKSVSVLWAMGTDIQRARIESAQAKAVFAALDYALRTGLIVTR
RGHNGGQRETPAELMAGTFLHTTSRAGDPQIHTHAVLMNVCRRKDGSTGTVDNRELLLHQQEMGAVYRLALSEALERDIG
LTTLKDGRNFRVAGVAPSVEERFSKRRAAIEQEARKRGIQTAHHREAAEHISRNTRAAKSDLPSRAVLRARWEQEC
>Mature_315_residues
SDVEYYIESIDEGLAAQTALTGERERPARGHRKRGDWSGRDRAAYYVDNGTGEKPGIWWTNVLKAPVQPLPFVCQSAEVS
PSDFRALARGCDPLTGQALVQSKGQRRVGYDLQFAAPKSVSVLWAMGTDIQRARIESAQAKAVFAALDYALRTGLIVTRR
GHNGGQRETPAELMAGTFLHTTSRAGDPQIHTHAVLMNVCRRKDGSTGTVDNRELLLHQQEMGAVYRLALSEALERDIGL
TTLKDGRNFRVAGVAPSVEERFSKRRAAIEQEARKRGIQTAHHREAAEHISRNTRAAKSDLPSRAVLRARWEQEC

Specific function: TraI has been identified as DNA helicase I and it also has an additional activity of site-specific nicking at oriT. DNA helicase I is a potent DNA-dependent ATPase [H]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traI of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014059
- InterPro:   IPR014129
- InterPro:   IPR009767
- InterPro:   IPR014862 [H]

Pfam domain/function: PF07057 TraI; PF08751 TrwC [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 35025; Mature: 34894

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVEYYIESIDEGLAAQTALTGERERPARGHRKRGDWSGRDRAAYYVDNGTGEKPGIWW
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
TNVLKAPVQPLPFVCQSAEVSPSDFRALARGCDPLTGQALVQSKGQRRVGYDLQFAAPKS
HHHHHCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEECCCCC
VSVLWAMGTDIQRARIESAQAKAVFAALDYALRTGLIVTRRGHNGGQRETPAELMAGTFL
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHH
HTTSRAGDPQIHTHAVLMNVCRRKDGSTGTVDNRELLLHQQEMGAVYRLALSEALERDIG
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTTLKDGRNFRVAGVAPSVEERFSKRRAAIEQEARKRGIQTAHHREAAEHISRNTRAAKS
CEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
DLPSRAVLRARWEQEC
CCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SDVEYYIESIDEGLAAQTALTGERERPARGHRKRGDWSGRDRAAYYVDNGTGEKPGIWW
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
TNVLKAPVQPLPFVCQSAEVSPSDFRALARGCDPLTGQALVQSKGQRRVGYDLQFAAPKS
HHHHHCCCCCCCHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCEEECCCCC
VSVLWAMGTDIQRARIESAQAKAVFAALDYALRTGLIVTRRGHNGGQRETPAELMAGTFL
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHH
HTTSRAGDPQIHTHAVLMNVCRRKDGSTGTVDNRELLLHQQEMGAVYRLALSEALERDIG
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTTLKDGRNFRVAGVAPSVEERFSKRRAAIEQEARKRGIQTAHHREAAEHISRNTRAAKS
CEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
DLPSRAVLRARWEQEC
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2163400; 7915817; 2680768; 2164585; 8736534 [H]