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The map label for this gene is rbsB [H]

Identifier: 162147281

GI number: 162147281

Start: 1492334

End: 1493326

Strand: Reverse

Name: rbsB [H]

Synonym: GDI_1486

Alternate gene names: 162147281

Gene position: 1493326-1492334 (Counterclockwise)

Preceding gene: 162147282

Following gene: 162147280

Centisome position: 37.86

GC content: 68.58

Gene sequence:

>993_bases
ATGCGCCCAGCGCACGTCCCGCCGACCTGCCATGGATCGGCACGCCCGTCCCGCCGGAAGATCCTGCTGCTGGCGGGGCT
GCTCGCCCTTGGTAGCACCGCCGCCGCGCGCGCGGAGGATTCGGTTCACAAGATCGGGGTCACCGTCGGCTCGCTCGGCA
ATCCGTTCTTCATCGCCGCCATCAGGGGTATTACGCAACAGGCCAAGGCCCTGGGTCCCACCGCGCCGGTGCAGAGCGTC
TCGGCCGATTACGATATCGGCAAGCAGGCGTCGCAGGTCGATTCCTTCATCGCCGCCGGCAACGACCTGGTGATGTTCAA
CGCGGTGGACTACCAGGCCGCCGCCCCGCTGGTCCGTCGCCTGCATGACAGCGGCGCGACCGTGGTCGCCTTCGATGTCG
GCGCGCCCGGCGCGGATGCGACGATCACCACCAACAACGTCAAGGCCGGCGAAATCTCGTGCCAGTATATCGTCGATCGC
CTGCATGGGCACGGTGACGTGGTGATCGTCAACGGGCCGCCCGTCACCGCGCTGAGCGACCGCGTGAAGGGCTGCCAGTC
GGTGTTCGCCAAGGCGCCGGGGATCAGGCTGCTGTCCTCCACGCTCGACGCCAAGGGCTCGCGCGACGGCGGACTGGCGG
TGGGGCAGAGCGTGATGGTGCGCTTCGACAAGGTGGACGCGATCTTCGCGGTCAACGACCCGACCGCGCTGGGCGTCGAA
CTGGCGGCACGCCAGGCGGGACGGCACGATTTCTTCCTGGCCTCGGTCGACGGCTCGCCCGACGTGGAACAGGAAATGCG
CGGCGGCAACACGCTGATCGCGGCCACGGCGTCGCAGGACCCGTTCGCCATCGCGACCACCGCCACCCGCATCGGGTTCG
ATATCCGGGCCGGACGGATCAAGCCGGGCATCGTCCGGCTGCTGGCCCCCAGCCTGATCACCCATGACACGATCGGCGGC
TACAAGGGCTGGAACGCGCCGCACAATAGCTGA

Upstream 100 bases:

>100_bases
AGTACGTCACCCGCTGGCGGCACCTGGCCGACCAGCGCACCAGGAGCCGGACCGAACCGGCCGCACCGGCGCCAGACGCG
CCCAATAAAGGATAATCATG

Downstream 100 bases:

>100_bases
CCGGCCGTGTCTTTTTTCCAGAACATCAACAAGAACAAGGAAAACAACCATGACCGCTCCAGGCTGCACCCTGACCGCGA
CCCTGCGCGCCCGGCCCGAG

Product: D-ribose-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MRPAHVPPTCHGSARPSRRKILLLAGLLALGSTAAARAEDSVHKIGVTVGSLGNPFFIAAIRGITQQAKALGPTAPVQSV
SADYDIGKQASQVDSFIAAGNDLVMFNAVDYQAAAPLVRRLHDSGATVVAFDVGAPGADATITTNNVKAGEISCQYIVDR
LHGHGDVVIVNGPPVTALSDRVKGCQSVFAKAPGIRLLSSTLDAKGSRDGGLAVGQSVMVRFDKVDAIFAVNDPTALGVE
LAARQAGRHDFFLASVDGSPDVEQEMRGGNTLIAATASQDPFAIATTATRIGFDIRAGRIKPGIVRLLAPSLITHDTIGG
YKGWNAPHNS

Sequences:

>Translated_330_residues
MRPAHVPPTCHGSARPSRRKILLLAGLLALGSTAAARAEDSVHKIGVTVGSLGNPFFIAAIRGITQQAKALGPTAPVQSV
SADYDIGKQASQVDSFIAAGNDLVMFNAVDYQAAAPLVRRLHDSGATVVAFDVGAPGADATITTNNVKAGEISCQYIVDR
LHGHGDVVIVNGPPVTALSDRVKGCQSVFAKAPGIRLLSSTLDAKGSRDGGLAVGQSVMVRFDKVDAIFAVNDPTALGVE
LAARQAGRHDFFLASVDGSPDVEQEMRGGNTLIAATASQDPFAIATTATRIGFDIRAGRIKPGIVRLLAPSLITHDTIGG
YKGWNAPHNS
>Mature_330_residues
MRPAHVPPTCHGSARPSRRKILLLAGLLALGSTAAARAEDSVHKIGVTVGSLGNPFFIAAIRGITQQAKALGPTAPVQSV
SADYDIGKQASQVDSFIAAGNDLVMFNAVDYQAAAPLVRRLHDSGATVVAFDVGAPGADATITTNNVKAGEISCQYIVDR
LHGHGDVVIVNGPPVTALSDRVKGCQSVFAKAPGIRLLSSTLDAKGSRDGGLAVGQSVMVRFDKVDAIFAVNDPTALGVE
LAARQAGRHDFFLASVDGSPDVEQEMRGGNTLIAATASQDPFAIATTATRIGFDIRAGRIKPGIVRLLAPSLITHDTIGG
YKGWNAPHNS

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=271, Percent_Identity=28.4132841328413, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790526, Length=292, Percent_Identity=26.7123287671233, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34199; Mature: 34199

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAHVPPTCHGSARPSRRKILLLAGLLALGSTAAARAEDSVHKIGVTVGSLGNPFFIAA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHEEEEEECCCCCCEEEHH
IRGITQQAKALGPTAPVQSVSADYDIGKQASQVDSFIAAGNDLVMFNAVDYQAAAPLVRR
HHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
LHDSGATVVAFDVGAPGADATITTNNVKAGEISCQYIVDRLHGHGDVVIVNGPPVTALSD
HHCCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHH
RVKGCQSVFAKAPGIRLLSSTLDAKGSRDGGLAVGQSVMVRFDKVDAIFAVNDPTALGVE
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHCHHHEEEEECCEEEEEECCCCHHHHH
LAARQAGRHDFFLASVDGSPDVEQEMRGGNTLIAATASQDPFAIATTATRIGFDIRAGRI
HHHHHCCCCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCEEEEEEHHHHCEEEECCCC
KPGIVRLLAPSLITHDTIGGYKGWNAPHNS
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPAHVPPTCHGSARPSRRKILLLAGLLALGSTAAARAEDSVHKIGVTVGSLGNPFFIAA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHEEEEEECCCCCCEEEHH
IRGITQQAKALGPTAPVQSVSADYDIGKQASQVDSFIAAGNDLVMFNAVDYQAAAPLVRR
HHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
LHDSGATVVAFDVGAPGADATITTNNVKAGEISCQYIVDRLHGHGDVVIVNGPPVTALSD
HHCCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHH
RVKGCQSVFAKAPGIRLLSSTLDAKGSRDGGLAVGQSVMVRFDKVDAIFAVNDPTALGVE
HHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHCHHHEEEEECCEEEEEECCCCHHHHH
LAARQAGRHDFFLASVDGSPDVEQEMRGGNTLIAATASQDPFAIATTATRIGFDIRAGRI
HHHHHCCCCEEEEEECCCCCCHHHHHCCCCEEEEEECCCCCEEEEEEHHHHCEEEECCCC
KPGIVRLLAPSLITHDTIGGYKGWNAPHNS
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]