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The map label for this gene is cysK [H]

Identifier: 162147275

GI number: 162147275

Start: 1488331

End: 1489317

Strand: Direct

Name: cysK [H]

Synonym: GDI_1480

Alternate gene names: 162147275

Gene position: 1488331-1489317 (Clockwise)

Preceding gene: 162147267

Following gene: 162147284

Centisome position: 37.74

GC content: 71.23

Gene sequence:

>987_bases
ATGGACAACACGGCGGTGGACGACCGGCTGGGCCTGGCGCGCCCGCGGGGGCGGGTGTACGATTCGATCGTCGATACGGT
CGGCGGCACGCCGCTGGTGGCCCTGCCGCACCTGACGCGCGAGGACGAGATTGCCAGCCGCATCCTGCTGAAGCTGGAAT
TCTTCAACCCGCTGGGATCGGTCAAGGACCGCATCGGCGCGGCCATGGTGCTGGCCGCCGAACAGCGGGGCGACATCACG
CCCGGCCGGACGGTGCTGGTCGAGCCGACATCGGGCAATACCGGCATTTCGCTGGCCTTCGTCGCCGCCGCGCGCGGCTA
TCGCCTGATCGTGACCATGCCCGAGGGCGCGTCGCTGGAGCGGCGCAAGATGCTGCGCCTGCTGGATGCGGAACTGGAAC
TGACGCCGGCCCGACTGGGCATGGCCGGGGCCATCGCCCGGGCCGAGGACATCCTGAACAATACGCCCGACGCCTGGATG
CCGCGCCAGTTCGACAATCCCGACAACCCGGCCGTCCACGCCGCCACCACGGCCGAGGAAATCTGGACCGACACGGACGG
CGCGGTGGATATCGTGGTGGCGGGCGTGGGCACCGGCGGCACCGCCAGCGGCATCGCCCATGCGCTGAAGCCGCGCAAGC
CTGGCCTGGCGGTCTATGGGGTGGAACCGGTCGAAAGCGCGATCCTGAACGGGGACGAGGCCGGGCCCCACGGCATCCAG
GGCATCGGCCCCGGCTTCTGCCCCCGCACGCTGGACCTGCCGGCGCTGGACGGCGTGCTGACGGTGTCCGAGCGCGAGGC
CATCGCCGCCGCCCGCCGCTGCGCGCGGCTGGACGGGGTGCCGATCGGCATTTCCTCGGGCGCCGCGCTGCATGCTGCCC
TGAAGCTGGCCGAACGGCCGGAAAACGCGGGCAAGACGATCGTGGTCATCGCGCCCTCGTTCGCCGAACGCTACCTGTCC
ACGTCGCTGTTCACCGGCCTGGCCTGA

Upstream 100 bases:

>100_bases
TGATCCACGCTTCTGTTTGGTATGAAGGTGGATTATGTCGAAGATTACGATTAAATCCGTGCCAGGGACTGCGCATACGG
ATTGAAGGAAGGACCGACCC

Downstream 100 bases:

>100_bases
CGTTCCGGTGTCGGCCCCTGCGCTGTCTTCTATGAGGACTGCGCGGTCACGTCGATGTCGACACCGACGTCCAGCGCCAC
CCACTGGCCGCTTGCCCATG

Product: cysteine synthase

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MDNTAVDDRLGLARPRGRVYDSIVDTVGGTPLVALPHLTREDEIASRILLKLEFFNPLGSVKDRIGAAMVLAAEQRGDIT
PGRTVLVEPTSGNTGISLAFVAAARGYRLIVTMPEGASLERRKMLRLLDAELELTPARLGMAGAIARAEDILNNTPDAWM
PRQFDNPDNPAVHAATTAEEIWTDTDGAVDIVVAGVGTGGTASGIAHALKPRKPGLAVYGVEPVESAILNGDEAGPHGIQ
GIGPGFCPRTLDLPALDGVLTVSEREAIAAARRCARLDGVPIGISSGAALHAALKLAERPENAGKTIVVIAPSFAERYLS
TSLFTGLA

Sequences:

>Translated_328_residues
MDNTAVDDRLGLARPRGRVYDSIVDTVGGTPLVALPHLTREDEIASRILLKLEFFNPLGSVKDRIGAAMVLAAEQRGDIT
PGRTVLVEPTSGNTGISLAFVAAARGYRLIVTMPEGASLERRKMLRLLDAELELTPARLGMAGAIARAEDILNNTPDAWM
PRQFDNPDNPAVHAATTAEEIWTDTDGAVDIVVAGVGTGGTASGIAHALKPRKPGLAVYGVEPVESAILNGDEAGPHGIQ
GIGPGFCPRTLDLPALDGVLTVSEREAIAAARRCARLDGVPIGISSGAALHAALKLAERPENAGKTIVVIAPSFAERYLS
TSLFTGLA
>Mature_328_residues
MDNTAVDDRLGLARPRGRVYDSIVDTVGGTPLVALPHLTREDEIASRILLKLEFFNPLGSVKDRIGAAMVLAAEQRGDIT
PGRTVLVEPTSGNTGISLAFVAAARGYRLIVTMPEGASLERRKMLRLLDAELELTPARLGMAGAIARAEDILNNTPDAWM
PRQFDNPDNPAVHAATTAEEIWTDTDGAVDIVVAGVGTGGTASGIAHALKPRKPGLAVYGVEPVESAILNGDEAGPHGIQ
GIGPGFCPRTLDLPALDGVLTVSEREAIAAARRCARLDGVPIGISSGAALHAALKLAERPENAGKTIVVIAPSFAERYLS
TSLFTGLA

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=314, Percent_Identity=38.5350318471338, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI295821200, Length=314, Percent_Identity=38.5350318471338, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI4557415, Length=314, Percent_Identity=38.5350318471338, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1788754, Length=319, Percent_Identity=50.7836990595611, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI2367138, Length=303, Percent_Identity=37.6237623762376, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=46.8646864686469, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI115535073, Length=312, Percent_Identity=50.6410256410256, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17562970, Length=301, Percent_Identity=46.843853820598, Blast_Score=267, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=47.4576271186441, Blast_Score=261, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17561720, Length=309, Percent_Identity=47.8964401294498, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI17534315, Length=325, Percent_Identity=40.3076923076923, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=45.0704225352113, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI25147552, Length=326, Percent_Identity=36.8098159509202, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17561716, Length=119, Percent_Identity=47.0588235294118, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321594, Length=314, Percent_Identity=37.2611464968153, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6321449, Length=336, Percent_Identity=31.8452380952381, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24643623, Length=313, Percent_Identity=37.6996805111821, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI20129101, Length=313, Percent_Identity=37.6996805111821, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34314; Mature: 34314

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNTAVDDRLGLARPRGRVYDSIVDTVGGTPLVALPHLTREDEIASRILLKLEFFNPLGS
CCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHH
VKDRIGAAMVLAAEQRGDITPGRTVLVEPTSGNTGISLAFVAAARGYRLIVTMPEGASLE
HHHHHCCEEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCCCHH
RRKMLRLLDAELELTPARLGMAGAIARAEDILNNTPDAWMPRQFDNPDNPAVHAATTAEE
HHHHHHHHHCCCEECHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCHHH
IWTDTDGAVDIVVAGVGTGGTASGIAHALKPRKPGLAVYGVEPVESAILNGDEAGPHGIQ
HHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCCCCCCCCC
GIGPGFCPRTLDLPALDGVLTVSEREAIAAARRCARLDGVPIGISSGAALHAALKLAERP
CCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCC
ENAGKTIVVIAPSFAERYLSTSLFTGLA
CCCCCEEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDNTAVDDRLGLARPRGRVYDSIVDTVGGTPLVALPHLTREDEIASRILLKLEFFNPLGS
CCCCCCCHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHH
VKDRIGAAMVLAAEQRGDITPGRTVLVEPTSGNTGISLAFVAAARGYRLIVTMPEGASLE
HHHHHCCEEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEECCCCCCHH
RRKMLRLLDAELELTPARLGMAGAIARAEDILNNTPDAWMPRQFDNPDNPAVHAATTAEE
HHHHHHHHHCCCEECHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCHHH
IWTDTDGAVDIVVAGVGTGGTASGIAHALKPRKPGLAVYGVEPVESAILNGDEAGPHGIQ
HHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCCCCCCCCC
GIGPGFCPRTLDLPALDGVLTVSEREAIAAARRCARLDGVPIGISSGAALHAALKLAERP
CCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCC
ENAGKTIVVIAPSFAERYLSTSLFTGLA
CCCCCEEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]