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The map label for this gene is ghrA [H]

Identifier: 162147268

GI number: 162147268

Start: 1480395

End: 1481351

Strand: Reverse

Name: ghrA [H]

Synonym: GDI_1473

Alternate gene names: 162147268

Gene position: 1481351-1480395 (Counterclockwise)

Preceding gene: 162147269

Following gene: 162147266

Centisome position: 37.56

GC content: 71.06

Gene sequence:

>957_bases
CTGATCGGACGGGAGCGGGACATGAAGGATGCGGCGGTCTTCTACAGCCAGGTGGACAACCCCGAACCGTGGCGGGCCGC
ATTGGCCGGCCTGCTGCCGGACCTGGATTTCGTCCTGGCCGACGACGTGACCGACCCCGCCGCCGTCCGCTATGCCCTGG
TCTGGAGCCCGCCGCGCGGCTTCTTCGCGCGCTTTCCCAACCTGCGGATGATCGTCGCCCTGGGGGCCGGGGTGGACGCG
CTGGTCACACGCGACGACCTGCCGCCGGGGGTTGCGATCACCCGGCTGCACGACCCGCTGATGTCGCGGATGATGGCCAG
CTACGTGCTGTTCGCGACCCTGCGCCTGGCGCGCGACATGCCGGCGTTCGAGGCCGCGCAGCGCCGGGGACAATGGCACT
TCATCGAACCCCGGCCGCTGAACCTGACGCGGGTGGGCGTGATGGGCCTGGGCGAACTGGGCCTGCTGGCGGCACGTGAG
TGCGCGCGGCAGGGCTTTACCGTGCGCGGCTGGTCGCGCAGCCCGAAATCCGACCCCGCCATCACCTGCTATTCCGGCAT
GGACAGCCTGCCGGACTTCCTGGGCGGCTGCGACATCCTGGTCTGCCTGCTGCCCAAGACACCGCAGACCATCGGGCTGC
TGGACGCCGAACGGCTGGCGATGCTTCCGCGCGGGGCGGCATTCGTCAATGTCGCGCGCGGCGCCATCGTCGATCAGGAC
GCGCTGGTCGCGGCCCTGGAATCGGGGCAGATCGGCCAGGCGACGCTGGACGTGTTCGCGCAGGAACCGCTGCCCGGGGA
CCATCCGCTCTGGCGGATGGAGAACGTCCTGATCACCCCGCACGTGGCCTCGGCCGCGCTGCCGGACTCGGCCGCGCCGC
AGGTGGCGGAAAACATCCGCCGCCTGCGCGCGGACCTGCCGGTCACGGACGCGGTGGACGTCGCACGCGGCTATTAG

Upstream 100 bases:

>100_bases
TCGCGCGAAAAGCGGCGCAAGGCGAAGCGCTAAACCACGGGGCGCGGTGACAAGGCCCCCTTCCCAGCCGCCTTGCGATG
CCGATAGGATCGGACCCGAA

Downstream 100 bases:

>100_bases
AGGTCCCGCGCGCCCTCCCGGCATGCTGTGTCTTTCCGGATACAGCCCACGCGCCTTCCCGTCGTGACGGTGCAAAATCG
CAATGAATCGCCGGTCCGCA

Product: D-3-phosphoglycerate dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MIGRERDMKDAAVFYSQVDNPEPWRAALAGLLPDLDFVLADDVTDPAAVRYALVWSPPRGFFARFPNLRMIVALGAGVDA
LVTRDDLPPGVAITRLHDPLMSRMMASYVLFATLRLARDMPAFEAAQRRGQWHFIEPRPLNLTRVGVMGLGELGLLAARE
CARQGFTVRGWSRSPKSDPAITCYSGMDSLPDFLGGCDILVCLLPKTPQTIGLLDAERLAMLPRGAAFVNVARGAIVDQD
ALVAALESGQIGQATLDVFAQEPLPGDHPLWRMENVLITPHVASAALPDSAAPQVAENIRRLRADLPVTDAVDVARGY

Sequences:

>Translated_318_residues
MIGRERDMKDAAVFYSQVDNPEPWRAALAGLLPDLDFVLADDVTDPAAVRYALVWSPPRGFFARFPNLRMIVALGAGVDA
LVTRDDLPPGVAITRLHDPLMSRMMASYVLFATLRLARDMPAFEAAQRRGQWHFIEPRPLNLTRVGVMGLGELGLLAARE
CARQGFTVRGWSRSPKSDPAITCYSGMDSLPDFLGGCDILVCLLPKTPQTIGLLDAERLAMLPRGAAFVNVARGAIVDQD
ALVAALESGQIGQATLDVFAQEPLPGDHPLWRMENVLITPHVASAALPDSAAPQVAENIRRLRADLPVTDAVDVARGY
>Mature_318_residues
MIGRERDMKDAAVFYSQVDNPEPWRAALAGLLPDLDFVLADDVTDPAAVRYALVWSPPRGFFARFPNLRMIVALGAGVDA
LVTRDDLPPGVAITRLHDPLMSRMMASYVLFATLRLARDMPAFEAAQRRGQWHFIEPRPLNLTRVGVMGLGELGLLAARE
CARQGFTVRGWSRSPKSDPAITCYSGMDSLPDFLGGCDILVCLLPKTPQTIGLLDAERLAMLPRGAAFVNVARGAIVDQD
ALVAALESGQIGQATLDVFAQEPLPGDHPLWRMENVLITPHVASAALPDSAAPQVAENIRRLRADLPVTDAVDVARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=228, Percent_Identity=30.7017543859649, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI23308577, Length=227, Percent_Identity=32.15859030837, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI87081824, Length=311, Percent_Identity=36.0128617363344, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI87082289, Length=242, Percent_Identity=30.5785123966942, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1789279, Length=190, Percent_Identity=27.3684210526316, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=245, Percent_Identity=30.2040816326531, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324055, Length=163, Percent_Identity=30.6748466257669, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325144, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322116, Length=235, Percent_Identity=23.8297872340426, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320925, Length=199, Percent_Identity=24.6231155778894, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28571528, Length=171, Percent_Identity=32.7485380116959, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28574286, Length=148, Percent_Identity=32.4324324324324, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI28574284, Length=265, Percent_Identity=27.1698113207547, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585514, Length=265, Percent_Identity=27.1698113207547, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28574282, Length=265, Percent_Identity=27.1698113207547, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45552429, Length=265, Percent_Identity=27.1698113207547, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45551003, Length=265, Percent_Identity=27.1698113207547, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585516, Length=102, Percent_Identity=36.2745098039216, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921140, Length=252, Percent_Identity=27.3809523809524, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34530; Mature: 34530

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRERDMKDAAVFYSQVDNPEPWRAALAGLLPDLDFVLADDVTDPAAVRYALVWSPPRG
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCH
FFARFPNLRMIVALGAGVDALVTRDDLPPGVAITRLHDPLMSRMMASYVLFATLRLARDM
HHHHCCCCEEEEEECCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAFEAAQRRGQWHFIEPRPLNLTRVGVMGLGELGLLAARECARQGFTVRGWSRSPKSDPA
CHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCC
ITCYSGMDSLPDFLGGCDILVCLLPKTPQTIGLLDAERLAMLPRGAAFVNVARGAIVDQD
EEEECCCHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHCCCEECHH
ALVAALESGQIGQATLDVFAQEPLPGDHPLWRMENVLITPHVASAALPDSAAPQVAENIR
HHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHCCEEECCCHHHHCCCCCCCHHHHHHHH
RLRADLPVTDAVDVARGY
HHHHCCCCCHHHHHHCCC
>Mature Secondary Structure
MIGRERDMKDAAVFYSQVDNPEPWRAALAGLLPDLDFVLADDVTDPAAVRYALVWSPPRG
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCH
FFARFPNLRMIVALGAGVDALVTRDDLPPGVAITRLHDPLMSRMMASYVLFATLRLARDM
HHHHCCCCEEEEEECCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAFEAAQRRGQWHFIEPRPLNLTRVGVMGLGELGLLAARECARQGFTVRGWSRSPKSDPA
CHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCC
ITCYSGMDSLPDFLGGCDILVCLLPKTPQTIGLLDAERLAMLPRGAAFVNVARGAIVDQD
EEEECCCHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHCCCEECHH
ALVAALESGQIGQATLDVFAQEPLPGDHPLWRMENVLITPHVASAALPDSAAPQVAENIR
HHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHCCEEECCCHHHHCCCCCCCHHHHHHHH
RLRADLPVTDAVDVARGY
HHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA