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The map label for this gene is rne [C]

Identifier: 162147232

GI number: 162147232

Start: 1440853

End: 1441944

Strand: Reverse

Name: rne [C]

Synonym: GDI_1437

Alternate gene names: 162147232

Gene position: 1441944-1440853 (Counterclockwise)

Preceding gene: 162147233

Following gene: 162147231

Centisome position: 36.56

GC content: 76.92

Gene sequence:

>1092_bases
CTGGCCGCCAGCGCGCCGGGCGAGATCCGCATCGCCGTCACCGACGACACCGGATTGCGCGACTACGCCCTGTGGCGCCC
GGGCCGCCCCGACGGCGTGGGCGACCTGCATCGCGGCCGCACCGGCGCGCGCATGCCCGCCCTGGGCGGCACCTTCATCG
ACCTGGCCGACGGCACCGCCGGCTTCCTGCCGGACTCGGCCGGCACGGCCCCGACCGAAGGCGATGCCGTGCTGGTCCGC
GTGGTCCGTGCCCCGCAGGGTGGCAAGGGCGCGCGCCTGAAGGCCGAAGGCGGGAGCGACGCGGCCGGGCCGCCCGCGCT
GCTGGCGCGTGGCCCCACCCCGCTGGACGCGCTGGCCCAGGCATGGCCGGATGCGCCGATCGTGATCGACGACGCCGCCA
TCGCCGCACGCCTCCCGCCCGGCCTGCGGCCCCGGATGCAACGTGTCCCCCGCGCCTTCGACGAGGAAACCGAGGCCGCG
TGCGCGGCACTGGCCGACCCGGTGGTGGCGCTGCCGGGCGGCATGCGCGCCAGCATCACCCCCACCCCGGCGCTGGTCGC
GATCGACATGGACGGCGGAGGCGCCAGCACCGACCGGCGCGCCAAGCAGACGGCGCAATTCGCCGCCAACCGCGACGCGC
TGCCGGCGTTGCTGCACCAGATCCGCCTGCGCAACCTGTCCGGCGCCATCGTGCTGGACGTGGCGGGACTGGCGATCCGC
AAGCGCCAGGCCCTGCGCGAGACGATCGAGGCCGCCTTGGCCCAAGACCCGCTGCGGCCGCGCTTCCTGGGCTTCACCGC
CCTGGGGCTGGCCGAAATCCTGCGGACCCGCATCCGCCCGCCGCTGCACGAGATGCTGTCCGGCGCGCCGGGCCGGGCGC
TGCAGATGCTGGATGACTGGGTCCGCCGGTCGGCGGGCCAGCCCGGACGGCCGCCGGGCCTGCGCGTGGGCACCGACCTG
GCCCGCGCCCTGGCCGAGGATCCGGACGCGATCGCCGACGCCCTGCGCCTGTCGGGCCACCAGCCGGCCTGGCGGCTGGA
CCCCGCCCTGGCGCCCCAGGGCTGGACATACGACCGGGACACACGACCATGA

Upstream 100 bases:

>100_bases
TGTCCGGCAGCCCCTCCGCCGTGATCGGCCTGCCGCTGTTCGAGGTCGCGCAGATGCTGCGCGGCCAGAAAGGGGACTGG
ATCCGGTGACGACGCGGATC

Downstream 100 bases:

>100_bases
CCGACCCCACCCCGACGAATACCGACCTTGCCCCCCTGCCCCCTTGCCCGATCTGCGGCCGGCCCGGGGCAGACGGCCTT
CCGTCCCTTCTGCTCGAAGC

Product: ribonuclease

Products: Cleaved RNA [C]

Alternate protein names: Ribonuclease E; Ribonuclease G; Ribonuclease G And E-Like Protein

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAASAPGEIRIAVTDDTGLRDYALWRPGRPDGVGDLHRGRTGARMPALGGTFIDLADGTAGFLPDSAGTAPTEGDAVLVR
VVRAPQGGKGARLKAEGGSDAAGPPALLARGPTPLDALAQAWPDAPIVIDDAAIAARLPPGLRPRMQRVPRAFDEETEAA
CAALADPVVALPGGMRASITPTPALVAIDMDGGGASTDRRAKQTAQFAANRDALPALLHQIRLRNLSGAIVLDVAGLAIR
KRQALRETIEAALAQDPLRPRFLGFTALGLAEILRTRIRPPLHEMLSGAPGRALQMLDDWVRRSAGQPGRPPGLRVGTDL
ARALAEDPDAIADALRLSGHQPAWRLDPALAPQGWTYDRDTRP

Sequences:

>Translated_363_residues
MAASAPGEIRIAVTDDTGLRDYALWRPGRPDGVGDLHRGRTGARMPALGGTFIDLADGTAGFLPDSAGTAPTEGDAVLVR
VVRAPQGGKGARLKAEGGSDAAGPPALLARGPTPLDALAQAWPDAPIVIDDAAIAARLPPGLRPRMQRVPRAFDEETEAA
CAALADPVVALPGGMRASITPTPALVAIDMDGGGASTDRRAKQTAQFAANRDALPALLHQIRLRNLSGAIVLDVAGLAIR
KRQALRETIEAALAQDPLRPRFLGFTALGLAEILRTRIRPPLHEMLSGAPGRALQMLDDWVRRSAGQPGRPPGLRVGTDL
ARALAEDPDAIADALRLSGHQPAWRLDPALAPQGWTYDRDTRP
>Mature_362_residues
AASAPGEIRIAVTDDTGLRDYALWRPGRPDGVGDLHRGRTGARMPALGGTFIDLADGTAGFLPDSAGTAPTEGDAVLVRV
VRAPQGGKGARLKAEGGSDAAGPPALLARGPTPLDALAQAWPDAPIVIDDAAIAARLPPGLRPRMQRVPRAFDEETEAAC
AALADPVVALPGGMRASITPTPALVAIDMDGGGASTDRRAKQTAQFAANRDALPALLHQIRLRNLSGAIVLDVAGLAIRK
RQALRETIEAALAQDPLRPRFLGFTALGLAEILRTRIRPPLHEMLSGAPGRALQMLDDWVRRSAGQPGRPPGLRVGTDLA
RALAEDPDAIADALRLSGHQPAWRLDPALAPQGWTYDRDTRP

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.4.- [C]

Molecular weight: Translated: 38072; Mature: 37941

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASAPGEIRIAVTDDTGLRDYALWRPGRPDGVGDLHRGRTGARMPALGGTFIDLADGTA
CCCCCCCCEEEEEECCCCCCHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCC
GFLPDSAGTAPTEGDAVLVRVVRAPQGGKGARLKAEGGSDAAGPPALLARGPTPLDALAQ
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCHHEECCCCCHHHHHH
AWPDAPIVIDDAAIAARLPPGLRPRMQRVPRAFDEETEAACAALADPVVALPGGMRASIT
HCCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCEECCC
PTPALVAIDMDGGGASTDRRAKQTAQFAANRDALPALLHQIRLRNLSGAIVLDVAGLAIR
CCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHH
KRQALRETIEAALAQDPLRPRFLGFTALGLAEILRTRIRPPLHEMLSGAPGRALQMLDDW
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHH
VRRSAGQPGRPPGLRVGTDLARALAEDPDAIADALRLSGHQPAWRLDPALAPQGWTYDRD
HHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCC
TRP
CCC
>Mature Secondary Structure 
AASAPGEIRIAVTDDTGLRDYALWRPGRPDGVGDLHRGRTGARMPALGGTFIDLADGTA
CCCCCCCEEEEEECCCCCCHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCC
GFLPDSAGTAPTEGDAVLVRVVRAPQGGKGARLKAEGGSDAAGPPALLARGPTPLDALAQ
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCHHEECCCCCHHHHHH
AWPDAPIVIDDAAIAARLPPGLRPRMQRVPRAFDEETEAACAALADPVVALPGGMRASIT
HCCCCCEEEECCHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCEECCCCCEECCC
PTPALVAIDMDGGGASTDRRAKQTAQFAANRDALPALLHQIRLRNLSGAIVLDVAGLAIR
CCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHH
KRQALRETIEAALAQDPLRPRFLGFTALGLAEILRTRIRPPLHEMLSGAPGRALQMLDDW
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHH
VRRSAGQPGRPPGLRVGTDLARALAEDPDAIADALRLSGHQPAWRLDPALAPQGWTYDRD
HHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCC
TRP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RNA [C]

Specific reaction: Cleaves RNA endonucleolytically in AU-rich single-strand regions [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA