Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 162147177

GI number: 162147177

Start: 1387895

End: 1388863

Strand: Direct

Name: prfB [H]

Synonym: GDI_1382

Alternate gene names: 162147177

Gene position: 1387895-1388863 (Clockwise)

Preceding gene: 162147176

Following gene: 162147178

Centisome position: 35.19

GC content: 67.39

Gene sequence:

>969_bases
ATGCGCGAGCGCACCCTGCTGGCCGGGCAGGTCGAGGGCGTGCAGCGCCTGGAAGCCGACGTCCGCGACACGCTGGACCT
GGTCGAACTGGCCGAAATGGAGGGCGATGACGCTGTCCTGAAGGACGCGGTGCAGATGCTGCGCGCCCTGGCCGAGGAGG
CCAAGCGCCGCGAGACCGAAAGCCTGCTGTCCGGCGAGGCCGACAGCAACGACTGCTATCTGGAAGTCAATGCCGGCGCC
GGCGGGACCGAAGCCCAGGACTGGGCCGAAATGCTGCTGCGCATGTACACCCGCTGGGCCGAGCAGCACGGTTACAAGGT
CGCGCTGATGGAAAGTTCGGAAGGCGAGCAGGCGGGGCTGAAATCGGCGACGATCCAGGTCACCGGCCCCAACGCCTATG
GCTGGCTGAAGACCGAGGCCGGAGTGCATCGCCTGGTGCGCATTTCGCCCTTCGACGCGGCGGCGCGGCGACAGACCTCG
TTCGCCTCGGTCTGGGTCTATCCGGTGGTGGATGATTCGATCGAGATCGAGATCAACGAAGCCGACCTGAAGGTCGATAC
GTTCCGTGCCTCGGGTGCCGGCGGCCAGCACGTCAACAAGACGGAATCGGCCATCCGCATCACTCATATCCCCACCGGGA
TCATCGTCGCCTGCCAGACCGACCGTTCGCAGCATCGCAACCGCGCCACGGCGATGACGATGCTGAAGGCGCGGATGTAC
GAGCAGGAACTGCAGCGGCGCGAGGCCGCGGCGGCGCAGACCGAGGCGGCGAAGACCGATATCGGCTGGGGACACCAGAT
CCGTTCCTACGTCCTGGCGCCCTATCAGCTGGTCAAGGACCTGCGTACGAGTGTCGAGCGCGGCAATCCCGACGCCGTGC
TGGACGGCGACCTGGACGACTTCATGGCCGCGGCCCTGGCCGCCCGGGTGGGCGCCACCCGGTCGGAGGCGAGCGCTTCC
GCCCAGTAA

Upstream 100 bases:

>100_bases
CATCTTTGACTGGGATGTCGCACTTGGCCGTCTCGCGGAACTGAACAACCGGGCGGAGGATCCGGATCTGTGGAACGATC
CCGACACCGCCCAGAAACTG

Downstream 100 bases:

>100_bases
CGGTGCCGCAGCAATGCCGCGAGGTCGCGCCGGATCGGGGCGGGGATGGCGGAGCCATCCCCGCCTTTTTTATGGCACGT
CCGCACGCGCATCCCGGGCG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRERTLLAGQVEGVQRLEADVRDTLDLVELAEMEGDDAVLKDAVQMLRALAEEAKRRETESLLSGEADSNDCYLEVNAGA
GGTEAQDWAEMLLRMYTRWAEQHGYKVALMESSEGEQAGLKSATIQVTGPNAYGWLKTEAGVHRLVRISPFDAAARRQTS
FASVWVYPVVDDSIEIEINEADLKVDTFRASGAGGQHVNKTESAIRITHIPTGIIVACQTDRSQHRNRATAMTMLKARMY
EQELQRREAAAAQTEAAKTDIGWGHQIRSYVLAPYQLVKDLRTSVERGNPDAVLDGDLDDFMAAALAARVGATRSEASAS
AQ

Sequences:

>Translated_322_residues
MRERTLLAGQVEGVQRLEADVRDTLDLVELAEMEGDDAVLKDAVQMLRALAEEAKRRETESLLSGEADSNDCYLEVNAGA
GGTEAQDWAEMLLRMYTRWAEQHGYKVALMESSEGEQAGLKSATIQVTGPNAYGWLKTEAGVHRLVRISPFDAAARRQTS
FASVWVYPVVDDSIEIEINEADLKVDTFRASGAGGQHVNKTESAIRITHIPTGIIVACQTDRSQHRNRATAMTMLKARMY
EQELQRREAAAAQTEAAKTDIGWGHQIRSYVLAPYQLVKDLRTSVERGNPDAVLDGDLDDFMAAALAARVGATRSEASAS
AQ
>Mature_322_residues
MRERTLLAGQVEGVQRLEADVRDTLDLVELAEMEGDDAVLKDAVQMLRALAEEAKRRETESLLSGEADSNDCYLEVNAGA
GGTEAQDWAEMLLRMYTRWAEQHGYKVALMESSEGEQAGLKSATIQVTGPNAYGWLKTEAGVHRLVRISPFDAAARRQTS
FASVWVYPVVDDSIEIEINEADLKVDTFRASGAGGQHVNKTESAIRITHIPTGIIVACQTDRSQHRNRATAMTMLKARMY
EQELQRREAAAAQTEAAKTDIGWGHQIRSYVLAPYQLVKDLRTSVERGNPDAVLDGDLDDFMAAALAARVGATRSEASAS
AQ

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=324, Percent_Identity=33.9506172839506, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI34577120, Length=220, Percent_Identity=37.7272727272727, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI166795305, Length=226, Percent_Identity=35.3982300884956, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI2367172, Length=307, Percent_Identity=56.0260586319218, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1787462, Length=298, Percent_Identity=39.5973154362416, Blast_Score=195, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=209, Percent_Identity=33.4928229665072, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=174, Percent_Identity=40.8045977011494, Blast_Score=136, Evalue=4e-33,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=43.8775510204082, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35313; Mature: 35313

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERTLLAGQVEGVQRLEADVRDTLDLVELAEMEGDDAVLKDAVQMLRALAEEAKRRETE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLLSGEADSNDCYLEVNAGAGGTEAQDWAEMLLRMYTRWAEQHGYKVALMESSEGEQAGL
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
KSATIQVTGPNAYGWLKTEAGVHRLVRISPFDAAARRQTSFASVWVYPVVDDSIEIEINE
CEEEEEEECCCCCEEEECHHHHHHEEEECCCCHHHHHCCCEEEEEEEEEECCCEEEEEEC
ADLKVDTFRASGAGGQHVNKTESAIRITHIPTGIIVACQTDRSQHRNRATAMTMLKARMY
CCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
EQELQRREAAAAQTEAAKTDIGWGHQIRSYVLAPYQLVKDLRTSVERGNPDAVLDGDLDD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
FMAAALAARVGATRSEASASAQ
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRERTLLAGQVEGVQRLEADVRDTLDLVELAEMEGDDAVLKDAVQMLRALAEEAKRRETE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLLSGEADSNDCYLEVNAGAGGTEAQDWAEMLLRMYTRWAEQHGYKVALMESSEGEQAGL
HHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
KSATIQVTGPNAYGWLKTEAGVHRLVRISPFDAAARRQTSFASVWVYPVVDDSIEIEINE
CEEEEEEECCCCCEEEECHHHHHHEEEECCCCHHHHHCCCEEEEEEEEEECCCEEEEEEC
ADLKVDTFRASGAGGQHVNKTESAIRITHIPTGIIVACQTDRSQHRNRATAMTMLKARMY
CCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
EQELQRREAAAAQTEAAKTDIGWGHQIRSYVLAPYQLVKDLRTSVERGNPDAVLDGDLDD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
FMAAALAARVGATRSEASASAQ
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA