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The map label for this gene is rph [H]

Identifier: 162147173

GI number: 162147173

Start: 1381918

End: 1382745

Strand: Reverse

Name: rph [H]

Synonym: GDI_1378

Alternate gene names: 162147173

Gene position: 1382745-1381918 (Counterclockwise)

Preceding gene: 162147185

Following gene: 162147172

Centisome position: 35.06

GC content: 71.26

Gene sequence:

>828_bases
ATGACAAGCACGGGACTGGAAATCCGTTCCCCGCTCGGGCTATGCCCGGCCCCTCGGCCTGCCGATCGGAGTACCGCCTT
CATGACCACCCGCCCCTCGGGCCGCGCACCTGATGCGCTGCGCGCCATTTCCATCGAACCGGGCTTCGCCCACCATGCCG
AGGGTTCGGCCCTGATCCGCATGGGCGGGACCGAGGTGCTGTGCGCCGCCACCGTCGAAAGCCGGGTGCCGCCGTTCCTG
CGCGGCCAGGGCAAGGGCTGGGTGACGGCCGAATACGGCATGCTGCCCCGCGCCACCCATACCCGTGGCGGCCGCGAGGC
CGCGAAGGGCAAGCAGTCGGGCCGCACCCAGGAAATCCAGCGCCTGATCGGCCGCTCGCTGCGTGCCGTCGTCGATCTGG
AGGCGCTGGGCGAGATGTCGATCACCGTCGATTGCGACGTGCTGAACGCCGATGGCGGTACCCGCTGCGCGTCGATCACC
GGCGCCTGGGTCGCATTGCGCCTGGCGCTGGAAACCCTGGTGCGCAACCGCGTGCTGGCCCGCCTGCCGCTGACGGCGCA
GGTCGCCGCCGTATCCTGCGGCCTGACCGGGGCCGGCGCGGTGCTGGACCTGGATTATGACGAGGACAGCGCCGCCGCCG
CCGACGCCAACTTCGTGCTGACCGAAACCGGCGGCATCGTCGAAATCCAGGCGACCGCCGAACACGCCCCGTTCGCGCAG
GACCAGTTCGACACGCTGCTGCGTCTGGCGCGAACCGGCACCGCGCAGCTTTTCATCGCCCAGCGCCAGGCCGTGGACGC
CGCCCTGACCGCGCGGACCGCACCGTGA

Upstream 100 bases:

>100_bases
CGTGGCGGGGTCCATCGGCCTTGGTTCCTCCCTGTTCGACCGGCCAGGAAACGCCAGCCCGAAAAAACGACCAGCCTAAA
TCTGGAACCGGAAGCGGGCC

Downstream 100 bases:

>100_bases
GCGGGCAGGCGACACGCCGCCTGGCGCGCGGCGACCGGCTGGTCCTGGCCAGCCACAATGCGGGAAAGCTGGTGGAATTT
TCCGCCCTGCTGGACGGATA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTSTGLEIRSPLGLCPAPRPADRSTAFMTTRPSGRAPDALRAISIEPGFAHHAEGSALIRMGGTEVLCAATVESRVPPFL
RGQGKGWVTAEYGMLPRATHTRGGREAAKGKQSGRTQEIQRLIGRSLRAVVDLEALGEMSITVDCDVLNADGGTRCASIT
GAWVALRLALETLVRNRVLARLPLTAQVAAVSCGLTGAGAVLDLDYDEDSAAAADANFVLTETGGIVEIQATAEHAPFAQ
DQFDTLLRLARTGTAQLFIAQRQAVDAALTARTAP

Sequences:

>Translated_275_residues
MTSTGLEIRSPLGLCPAPRPADRSTAFMTTRPSGRAPDALRAISIEPGFAHHAEGSALIRMGGTEVLCAATVESRVPPFL
RGQGKGWVTAEYGMLPRATHTRGGREAAKGKQSGRTQEIQRLIGRSLRAVVDLEALGEMSITVDCDVLNADGGTRCASIT
GAWVALRLALETLVRNRVLARLPLTAQVAAVSCGLTGAGAVLDLDYDEDSAAAADANFVLTETGGIVEIQATAEHAPFAQ
DQFDTLLRLARTGTAQLFIAQRQAVDAALTARTAP
>Mature_274_residues
TSTGLEIRSPLGLCPAPRPADRSTAFMTTRPSGRAPDALRAISIEPGFAHHAEGSALIRMGGTEVLCAATVESRVPPFLR
GQGKGWVTAEYGMLPRATHTRGGREAAKGKQSGRTQEIQRLIGRSLRAVVDLEALGEMSITVDCDVLNADGGTRCASITG
AWVALRLALETLVRNRVLARLPLTAQVAAVSCGLTGAGAVLDLDYDEDSAAAADANFVLTETGGIVEIQATAEHAPFAQD
QFDTLLRLARTGTAQLFIAQRQAVDAALTARTAP

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=60.4761904761905, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI71981632, Length=185, Percent_Identity=25.9459459459459, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 28872; Mature: 28741

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTGLEIRSPLGLCPAPRPADRSTAFMTTRPSGRAPDALRAISIEPGFAHHAEGSALIR
CCCCCCEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCEEEE
MGGTEVLCAATVESRVPPFLRGQGKGWVTAEYGMLPRATHTRGGREAAKGKQSGRTQEIQ
ECCCEEEEEEEHHHCCCHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCHHHHH
RLIGRSLRAVVDLEALGEMSITVDCDVLNADGGTRCASITGAWVALRLALETLVRNRVLA
HHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
RLPLTAQVAAVSCGLTGAGAVLDLDYDEDSAAAADANFVLTETGGIVEIQATAEHAPFAQ
HCCCHHEEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCH
DQFDTLLRLARTGTAQLFIAQRQAVDAALTARTAP
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSTGLEIRSPLGLCPAPRPADRSTAFMTTRPSGRAPDALRAISIEPGFAHHAEGSALIR
CCCCCEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCEEEE
MGGTEVLCAATVESRVPPFLRGQGKGWVTAEYGMLPRATHTRGGREAAKGKQSGRTQEIQ
ECCCEEEEEEEHHHCCCHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCHHHHH
RLIGRSLRAVVDLEALGEMSITVDCDVLNADGGTRCASITGAWVALRLALETLVRNRVLA
HHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
RLPLTAQVAAVSCGLTGAGAVLDLDYDEDSAAAADANFVLTETGGIVEIQATAEHAPFAQ
HCCCHHEEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCH
DQFDTLLRLARTGTAQLFIAQRQAVDAALTARTAP
HHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA