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The map label for this gene is pnp

Identifier: 162147166

GI number: 162147166

Start: 1372696

End: 1374834

Strand: Direct

Name: pnp

Synonym: GDI_1371

Alternate gene names: 162147166

Gene position: 1372696-1374834 (Clockwise)

Preceding gene: 162147165

Following gene: 162147167

Centisome position: 34.8

GC content: 66.62

Gene sequence:

>2139_bases
ATGTTCAATTACTACCGCAAGGAAATCGAGTGGGGCGGCCGTCCGCTGGTGCTGGAAACGGGCAAGATCGCCCGCCAGGC
CGACGGCGCCGTGGTCGTGACCTATGGCGACACGGTCGTGCTGTGCACCGCCGTCGGCGCCCGCAGCGTCAAGCCGGGCC
AGGATTTCTTCCCGCTGACCGTCAACTACCAGGAAAAGGCCTTCGCGGCCGGCAAGATCCCGGGTGGGTTCTTCAAGCGC
GAGGGCCGTCCGTCCGAGATCGAGGTCCTGAATTCCCGCCTGATCGACCGTCCGATCCGCCCCCTGTTCCCCGAGAACTT
CCGCAACGAGGTGCAGGTCGTAGCCACGGTGCTGAGCCACGACCTGGAGAACGATCCCGCGATCGCGGCGCTGATCGGCT
GCTCGGCCGCGCTGACGCTGTCGGGCATCCCGTTCTTCGGTCCGGTGGCGGCCTGCCGCGTCGGTTACGCGGACGGGGCC
TATATCCTGAACCCGACCCTGCCCGAAATGAAGGAAAGCGCGCTGGACCTGGTCGTCGCCGGTACGTCCGAAGGCGTGCT
GATGGTCGAGTCCGAGGCGAGCGAACTGAGCGAAGACGTCATGCTGGGCGCCGTGACCTTCGGGCATGAAGCGTTCCAGG
CCGTCATCGACGCCATCATCTCGCTGGCCGAGCATGCGGCCAAGGCACCGTGGGACCTGGCCGAGCCGTCGGCCGAGGAA
ATCGCGCTGAAGCAGCGGATCGACACGCTGGGCCGCGCCGCGATCGCCGAGGCGTATCAGGAGCGCGTGAAGCAGCAGCG
CTACAAGAAGGTCGGCGCCGCCAAGGAAGCCGTGCTGGCGGCCCTGGCCACCGAAGGGCTGGACGTCACCGCGGCCAAGC
CGATCCTGAAGGATCTGGAAGCCGACGTGGTGCGCAGCGCGGTGCTGGATACCGGCTACCGCATTGACGGCCGCGACCTG
AAGACGGTCCGCCCGATCGTCTCCGAGGTCGGCATCCTGCCGCGCGCCCATGGTTCGGCCCTGTTCACCCGTGGCGAGAC
CCAGGCCCTGGTCGTCGCCACCCTGGGCACCGCCCAGGACGAGCAGGTGATCGACGCGCTGGAAGGCGAATACCGCACCA
ACTTCATGCTGCACTACAACTTCCCCCCCTATTCGGTGGGTGAATGCGGCCGCATGGGCTCGCCCGGCCGGCGCGAGATC
GGGCATGGCAAGCTGGCCTGGCGCGCGATCCATCCGCTGCTGCCCGGCAAGGACACCTTCCCGTACACGATGCGCATCGT
GTCCGAGATCACCGAGAGCAACGGATCGTCCTCGATGGCCACGGTCTGCGGCACCTCGCTGGCGCTGATGGATGCGGGCG
TGCCGCTGAAGCGTCCGGTGGCGGGCATCGCCATGGGCCTGATCAAGGAAGATCGCGGCTTCGCCGTGCTGTCCGACATC
CTGGGTGACGAGGATCACCTGGGCGACATGGACTTCAAGGTCGCGGGCACCGAATCCGGCATCACCGCGCTGCAGATGGA
CATCAAGATCACGTCCATCACGCCCGAGATCATGAAGATCGCCCTGGGCCAGGCGCGCGACGGCCGCCTGCACATCCTGG
GCGAGATGTCCAAGGCGCTGACCGAAGGCCGTTCGGACGTGTCCTCCACCGCGCCGAAGATCACCACGATCTCGGTGCCG
AAGGAAAAGATCCGTGACGTGATCGGCCAGGGCGGCAAGGTGATCCGCGAGATCGTTGAATATTCGGGTGCCAAGATCGA
TATCAACGATGACGGCACGATCATGATCGCGGCCTCGTCCGAAGATCAGGCCACCCGCGCGATCGAACGGATCCGCGGCA
TCGTGGCCGAGCCCGAGCTGGGCGCGATCTACACCGGCAAGGTGGTCAAGACCGCCGATTTCGGTGCGTTCGTGAACTTC
CTGGGCGCGCGCGACGGCCTGGTCCATATCTCGGAACTGGCGCAGGGCCGGGTGGCGAAGACCACGGACGTCGTCAACCA
GGGCGACGTGGTGAAGGTCAAGGTCCTGGGCTTCGACGATCGCGGCAAGGTCAAGCTGTCGATGCGGGTCGTCGACCAGC
AGACCGGCGCGGACATCACCGAGTCCGTCGGCGAGCGCCCGGGCCGTCCGGCACGCTGA

Upstream 100 bases:

>100_bases
CAACCGTCATCGCTCCATGCCGTCCGAGACGCTGTCATTCCTCCCGTCGTCCGGGGACCTCCGACCCGCCTGGGCCGGCC
GCCGGACAGGGAATGTCTGA

Downstream 100 bases:

>100_bases
GGCCGGGCGCCAAGGCCGGGTGGCGGCGCGGGGCCCGGGTTCCGCACCGCCGCTTCCCGCCCGTCCGGGCGGGGCACACG
CAGTAAAGTCCCGGCCCAGC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MFNYYRKEIEWGGRPLVLETGKIARQADGAVVVTYGDTVVLCTAVGARSVKPGQDFFPLTVNYQEKAFAAGKIPGGFFKR
EGRPSEIEVLNSRLIDRPIRPLFPENFRNEVQVVATVLSHDLENDPAIAALIGCSAALTLSGIPFFGPVAACRVGYADGA
YILNPTLPEMKESALDLVVAGTSEGVLMVESEASELSEDVMLGAVTFGHEAFQAVIDAIISLAEHAAKAPWDLAEPSAEE
IALKQRIDTLGRAAIAEAYQERVKQQRYKKVGAAKEAVLAALATEGLDVTAAKPILKDLEADVVRSAVLDTGYRIDGRDL
KTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTAQDEQVIDALEGEYRTNFMLHYNFPPYSVGECGRMGSPGRREI
GHGKLAWRAIHPLLPGKDTFPYTMRIVSEITESNGSSSMATVCGTSLALMDAGVPLKRPVAGIAMGLIKEDRGFAVLSDI
LGDEDHLGDMDFKVAGTESGITALQMDIKITSITPEIMKIALGQARDGRLHILGEMSKALTEGRSDVSSTAPKITTISVP
KEKIRDVIGQGGKVIREIVEYSGAKIDINDDGTIMIAASSEDQATRAIERIRGIVAEPELGAIYTGKVVKTADFGAFVNF
LGARDGLVHISELAQGRVAKTTDVVNQGDVVKVKVLGFDDRGKVKLSMRVVDQQTGADITESVGERPGRPAR

Sequences:

>Translated_712_residues
MFNYYRKEIEWGGRPLVLETGKIARQADGAVVVTYGDTVVLCTAVGARSVKPGQDFFPLTVNYQEKAFAAGKIPGGFFKR
EGRPSEIEVLNSRLIDRPIRPLFPENFRNEVQVVATVLSHDLENDPAIAALIGCSAALTLSGIPFFGPVAACRVGYADGA
YILNPTLPEMKESALDLVVAGTSEGVLMVESEASELSEDVMLGAVTFGHEAFQAVIDAIISLAEHAAKAPWDLAEPSAEE
IALKQRIDTLGRAAIAEAYQERVKQQRYKKVGAAKEAVLAALATEGLDVTAAKPILKDLEADVVRSAVLDTGYRIDGRDL
KTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTAQDEQVIDALEGEYRTNFMLHYNFPPYSVGECGRMGSPGRREI
GHGKLAWRAIHPLLPGKDTFPYTMRIVSEITESNGSSSMATVCGTSLALMDAGVPLKRPVAGIAMGLIKEDRGFAVLSDI
LGDEDHLGDMDFKVAGTESGITALQMDIKITSITPEIMKIALGQARDGRLHILGEMSKALTEGRSDVSSTAPKITTISVP
KEKIRDVIGQGGKVIREIVEYSGAKIDINDDGTIMIAASSEDQATRAIERIRGIVAEPELGAIYTGKVVKTADFGAFVNF
LGARDGLVHISELAQGRVAKTTDVVNQGDVVKVKVLGFDDRGKVKLSMRVVDQQTGADITESVGERPGRPAR
>Mature_712_residues
MFNYYRKEIEWGGRPLVLETGKIARQADGAVVVTYGDTVVLCTAVGARSVKPGQDFFPLTVNYQEKAFAAGKIPGGFFKR
EGRPSEIEVLNSRLIDRPIRPLFPENFRNEVQVVATVLSHDLENDPAIAALIGCSAALTLSGIPFFGPVAACRVGYADGA
YILNPTLPEMKESALDLVVAGTSEGVLMVESEASELSEDVMLGAVTFGHEAFQAVIDAIISLAEHAAKAPWDLAEPSAEE
IALKQRIDTLGRAAIAEAYQERVKQQRYKKVGAAKEAVLAALATEGLDVTAAKPILKDLEADVVRSAVLDTGYRIDGRDL
KTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTAQDEQVIDALEGEYRTNFMLHYNFPPYSVGECGRMGSPGRREI
GHGKLAWRAIHPLLPGKDTFPYTMRIVSEITESNGSSSMATVCGTSLALMDAGVPLKRPVAGIAMGLIKEDRGFAVLSDI
LGDEDHLGDMDFKVAGTESGITALQMDIKITSITPEIMKIALGQARDGRLHILGEMSKALTEGRSDVSSTAPKITTISVP
KEKIRDVIGQGGKVIREIVEYSGAKIDINDDGTIMIAASSEDQATRAIERIRGIVAEPELGAIYTGKVVKTADFGAFVNF
LGARDGLVHISELAQGRVAKTTDVVNQGDVVKVKVLGFDDRGKVKLSMRVVDQQTGADITESVGERPGRPAR

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=728, Percent_Identity=37.2252747252747, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI4826690, Length=88, Percent_Identity=53.4090909090909, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI21361576, Length=86, Percent_Identity=44.1860465116279, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI145693187, Length=697, Percent_Identity=57.9626972740316, Blast_Score=786, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=712, Percent_Identity=32.0224719101124, Blast_Score=328, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17535281, Length=103, Percent_Identity=40.7766990291262, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320850, Length=118, Percent_Identity=38.135593220339, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281362905, Length=706, Percent_Identity=36.4022662889518, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651641, Length=706, Percent_Identity=36.4022662889518, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651643, Length=706, Percent_Identity=36.4022662889518, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI161079377, Length=656, Percent_Identity=35.6707317073171, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI20129977, Length=92, Percent_Identity=45.6521739130435, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_GLUDA (A9HF35)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601627.1
- RefSeq:   YP_002276450.1
- ProteinModelPortal:   A9HF35
- GeneID:   5792130
- GeneID:   6975502
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_2075
- HOGENOM:   HBG382411
- OMA:   LHISEIR
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76310; Mature: 76310

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNYYRKEIEWGGRPLVLETGKIARQADGAVVVTYGDTVVLCTAVGARSVKPGQDFFPLT
CCCHHHHHHHCCCCEEEEECCCHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCCEEEE
VNYQEKAFAAGKIPGGFFKREGRPSEIEVLNSRLIDRPIRPLFPENFRNEVQVVATVLSH
ECCCHHHEECCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
DLENDPAIAALIGCSAALTLSGIPFFGPVAACRVGYADGAYILNPTLPEMKESALDLVVA
CCCCCCHHHHHHHCHHHHHCCCCCCCCHHHHHHCCCCCCCEEECCCCHHHHHHHEEEEEE
GTSEGVLMVESEASELSEDVMLGAVTFGHEAFQAVIDAIISLAEHAAKAPWDLAEPSAEE
CCCCCEEEEECCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IALKQRIDTLGRAAIAEAYQERVKQQRYKKVGAAKEAVLAALATEGLDVTAAKPILKDLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ADVVRSAVLDTGYRIDGRDLKTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTAQD
HHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCCCH
EQVIDALEGEYRTNFMLHYNFPPYSVGECGRMGSPGRREIGHGKLAWRAIHPLLPGKDTF
HHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCC
PYTMRIVSEITESNGSSSMATVCGTSLALMDAGVPLKRPVAGIAMGLIKEDRGFAVLSDI
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
LGDEDHLGDMDFKVAGTESGITALQMDIKITSITPEIMKIALGQARDGRLHILGEMSKAL
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEECCHHHHHHHHCCCCCCCEEEHHHHHHHH
TEGRSDVSSTAPKITTISVPKEKIRDVIGQGGKVIREIVEYSGAKIDINDDGTIMIAASS
HHCCHHHHHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEECC
EDQATRAIERIRGIVAEPELGAIYTGKVVKTADFGAFVNFLGARDGLVHISELAQGRVAK
CHHHHHHHHHHHCCCCCCCCCEEEECCEEEECHHHHHHHHHCCCCCCEEHHHHHCCCCCH
TTDVVNQGDVVKVKVLGFDDRGKVKLSMRVVDQQTGADITESVGERPGRPAR
HHHHCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFNYYRKEIEWGGRPLVLETGKIARQADGAVVVTYGDTVVLCTAVGARSVKPGQDFFPLT
CCCHHHHHHHCCCCEEEEECCCHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCCEEEE
VNYQEKAFAAGKIPGGFFKREGRPSEIEVLNSRLIDRPIRPLFPENFRNEVQVVATVLSH
ECCCHHHEECCCCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
DLENDPAIAALIGCSAALTLSGIPFFGPVAACRVGYADGAYILNPTLPEMKESALDLVVA
CCCCCCHHHHHHHCHHHHHCCCCCCCCHHHHHHCCCCCCCEEECCCCHHHHHHHEEEEEE
GTSEGVLMVESEASELSEDVMLGAVTFGHEAFQAVIDAIISLAEHAAKAPWDLAEPSAEE
CCCCCEEEEECCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IALKQRIDTLGRAAIAEAYQERVKQQRYKKVGAAKEAVLAALATEGLDVTAAKPILKDLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ADVVRSAVLDTGYRIDGRDLKTVRPIVSEVGILPRAHGSALFTRGETQALVVATLGTAQD
HHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCCCH
EQVIDALEGEYRTNFMLHYNFPPYSVGECGRMGSPGRREIGHGKLAWRAIHPLLPGKDTF
HHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCC
PYTMRIVSEITESNGSSSMATVCGTSLALMDAGVPLKRPVAGIAMGLIKEDRGFAVLSDI
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
LGDEDHLGDMDFKVAGTESGITALQMDIKITSITPEIMKIALGQARDGRLHILGEMSKAL
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEECCHHHHHHHHCCCCCCCEEEHHHHHHHH
TEGRSDVSSTAPKITTISVPKEKIRDVIGQGGKVIREIVEYSGAKIDINDDGTIMIAASS
HHCCHHHHHCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEECC
EDQATRAIERIRGIVAEPELGAIYTGKVVKTADFGAFVNFLGARDGLVHISELAQGRVAK
CHHHHHHHHHHHCCCCCCCCCEEEECCEEEECHHHHHHHHHCCCCCCEEHHHHHCCCCCH
TTDVVNQGDVVKVKVLGFDDRGKVKLSMRVVDQQTGADITESVGERPGRPAR
HHHHCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA