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The map label for this gene is clpB [H]

Identifier: 162147155

GI number: 162147155

Start: 1361908

End: 1363176

Strand: Reverse

Name: clpB [H]

Synonym: GDI_1360

Alternate gene names: 162147155

Gene position: 1363176-1361908 (Counterclockwise)

Preceding gene: 162147156

Following gene: 162147149

Centisome position: 34.56

GC content: 66.82

Gene sequence:

>1269_bases
ATGAAGGCTCTTCTGCCCGCCCTGAACCGGCTGATGCCGGTGATGATGGTCATTTTCCTGATCCTGGCGAGCTTGCAGGC
GGCGGCGTCGCTGCATCTTTCTCTCGCCAGTCTGACACATTTCATGGAATGGTGTGCGCCGGCCTTTCCCGTGCTGACGG
CCGGGAGCAGCCTGCTGGTCCTGGCGGGTCTGATGTTCGAGACCCGCGCCGAACGGCTGGCGCGCAAGGGGCTGCGACGC
CGACGGGGGTGGATGATGGATGTACTGGCCAGGCTGACCAACCGCACCGCGCTGGAAGAAATGATGGCGCGCGAGCAGAA
GGAAACGGTCATCGACGCCGAGGAACTGGCGGCGAGCCTGCGGGCCCGGGTGATCGGGCAGGATCAGGTGTGCGAGGATA
TCGCCGCCCAGTTGCGCCGCCGCATGGCGCTGCAGGTGCGCGGCAAGCCGGTGGGCATCTTCCTGCTGGCCGGGCCGCCG
GGCACCGGCAAGACCTATCTGGCCAAGCAGATGGCCCGGCAACTGGACCGGCCGCTGCTGCATTTCGACATGACGCAGAT
GAGCTCGCCCCACGCCGCGACCCAGTTGTTCGGATCGCCCAAGGGCTATGTCGGGTCCGACACGTTCGGCAAGCTGACCG
GCGGGCTGAAGGAAAAGCCCGACGCCGTCGTGCTGCTGGACGAGATCGAGAAAGCGCATCCCGACGTCTTCAAGAAGTTC
CTGACCGCGTGGAACGACGGGCATGTCACCGAGGCCTCGACCGGCCAGCAGGTTTCGACCGTCCGCGCGATCTTCGTCCT
GACGTCCAACATCGCGACCGATGCCCTGTCGGAAATCGCCGACCGCCTGGCCGACGAACCCGACCGCATGCGCGCCGAAT
CGGTCGAGGCCCTGCGCCAGGCCGGATTCGCGCCCGAGGTGCTGAACCGGCTGGACCGGATCTTCGTCTTCCGTGCGCTG
ACCGGGCTGGATGTCGCCCGCGTCGCCGCGCTGGAAATCGAGGCGATGATCGAGAGTTACGGGTTGAGGGTGGAAACCGG
CGGCATCGATGCGTCGCTGCTGTTCGAGGTCATGCGGCGGCAGGATCGCATGGGGGCCTCGGCCAGCGCCCGCGACCTGG
TCCGTTCGATCGAGGACATGATGAGCGATTCGCTGATTTCCGCGCGGCAGCAGGGGGCCAAGCTGGTCCGCGTCGTATCG
GGCGAGAACGGCGTCACCGCGGAAATCGCGAACGACGCCGACGCGCAGCGGGCACGCGTGCTGCGCTGA

Upstream 100 bases:

>100_bases
GCGTCGCCGGGCTGGCGCCCGGGGCCATCACCGTCCTGGGGCCGACGCCGCTGCCCATTATCCATCGGGACGAGATGCTT
GTTCTGGATGTGATCGTCCA

Downstream 100 bases:

>100_bases
TCCGCTCCATCCCTTCGTCACGGTCCGTCTGAATGTCCGCTGCCTCCCGCCTCTGGAATCGCGCGCCGCTGTGGCGGAGC
TGCGTCTTCGGTATCCCGAT

Product: chaperone clpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKALLPALNRLMPVMMVIFLILASLQAAASLHLSLASLTHFMEWCAPAFPVLTAGSSLLVLAGLMFETRAERLARKGLRR
RRGWMMDVLARLTNRTALEEMMAREQKETVIDAEELAASLRARVIGQDQVCEDIAAQLRRRMALQVRGKPVGIFLLAGPP
GTGKTYLAKQMARQLDRPLLHFDMTQMSSPHAATQLFGSPKGYVGSDTFGKLTGGLKEKPDAVVLLDEIEKAHPDVFKKF
LTAWNDGHVTEASTGQQVSTVRAIFVLTSNIATDALSEIADRLADEPDRMRAESVEALRQAGFAPEVLNRLDRIFVFRAL
TGLDVARVAALEIEAMIESYGLRVETGGIDASLLFEVMRRQDRMGASASARDLVRSIEDMMSDSLISARQQGAKLVRVVS
GENGVTAEIANDADAQRARVLR

Sequences:

>Translated_422_residues
MKALLPALNRLMPVMMVIFLILASLQAAASLHLSLASLTHFMEWCAPAFPVLTAGSSLLVLAGLMFETRAERLARKGLRR
RRGWMMDVLARLTNRTALEEMMAREQKETVIDAEELAASLRARVIGQDQVCEDIAAQLRRRMALQVRGKPVGIFLLAGPP
GTGKTYLAKQMARQLDRPLLHFDMTQMSSPHAATQLFGSPKGYVGSDTFGKLTGGLKEKPDAVVLLDEIEKAHPDVFKKF
LTAWNDGHVTEASTGQQVSTVRAIFVLTSNIATDALSEIADRLADEPDRMRAESVEALRQAGFAPEVLNRLDRIFVFRAL
TGLDVARVAALEIEAMIESYGLRVETGGIDASLLFEVMRRQDRMGASASARDLVRSIEDMMSDSLISARQQGAKLVRVVS
GENGVTAEIANDADAQRARVLR
>Mature_422_residues
MKALLPALNRLMPVMMVIFLILASLQAAASLHLSLASLTHFMEWCAPAFPVLTAGSSLLVLAGLMFETRAERLARKGLRR
RRGWMMDVLARLTNRTALEEMMAREQKETVIDAEELAASLRARVIGQDQVCEDIAAQLRRRMALQVRGKPVGIFLLAGPP
GTGKTYLAKQMARQLDRPLLHFDMTQMSSPHAATQLFGSPKGYVGSDTFGKLTGGLKEKPDAVVLLDEIEKAHPDVFKKF
LTAWNDGHVTEASTGQQVSTVRAIFVLTSNIATDALSEIADRLADEPDRMRAESVEALRQAGFAPEVLNRLDRIFVFRAL
TGLDVARVAALEIEAMIESYGLRVETGGIDASLLFEVMRRQDRMGASASARDLVRSIEDMMSDSLISARQQGAKLVRVVS
GENGVTAEIANDADAQRARVLR

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=175, Percent_Identity=36, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788943, Length=331, Percent_Identity=30.2114803625378, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787109, Length=277, Percent_Identity=33.2129963898917, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320464, Length=213, Percent_Identity=38.0281690140845, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323002, Length=226, Percent_Identity=30.9734513274336, Blast_Score=104, Evalue=3e-23,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 46284; Mature: 46284

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALLPALNRLMPVMMVIFLILASLQAAASLHLSLASLTHFMEWCAPAFPVLTAGSSLLV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LAGLMFETRAERLARKGLRRRRGWMMDVLARLTNRTALEEMMAREQKETVIDAEELAASL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RARVIGQDQVCEDIAAQLRRRMALQVRGKPVGIFLLAGPPGTGKTYLAKQMARQLDRPLL
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHH
HFDMTQMSSPHAATQLFGSPKGYVGSDTFGKLTGGLKEKPDAVVLLDEIEKAHPDVFKKF
HHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCEEEEHHHHHHCCHHHHHHH
LTAWNDGHVTEASTGQQVSTVRAIFVLTSNIATDALSEIADRLADEPDRMRAESVEALRQ
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AGFAPEVLNRLDRIFVFRALTGLDVARVAALEIEAMIESYGLRVETGGIDASLLFEVMRR
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHH
QDRMGASASARDLVRSIEDMMSDSLISARQQGAKLVRVVSGENGVTAEIANDADAQRARV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCHHHHHHC
LR
CC
>Mature Secondary Structure
MKALLPALNRLMPVMMVIFLILASLQAAASLHLSLASLTHFMEWCAPAFPVLTAGSSLLV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LAGLMFETRAERLARKGLRRRRGWMMDVLARLTNRTALEEMMAREQKETVIDAEELAASL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RARVIGQDQVCEDIAAQLRRRMALQVRGKPVGIFLLAGPPGTGKTYLAKQMARQLDRPLL
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCHH
HFDMTQMSSPHAATQLFGSPKGYVGSDTFGKLTGGLKEKPDAVVLLDEIEKAHPDVFKKF
HHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCEEEEHHHHHHCCHHHHHHH
LTAWNDGHVTEASTGQQVSTVRAIFVLTSNIATDALSEIADRLADEPDRMRAESVEALRQ
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AGFAPEVLNRLDRIFVFRALTGLDVARVAALEIEAMIESYGLRVETGGIDASLLFEVMRR
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHH
QDRMGASASARDLVRSIEDMMSDSLISARQQGAKLVRVVSGENGVTAEIANDADAQRARV
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCHHHHHHC
LR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500908 [H]