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The map label for this gene is podA [H]

Identifier: 162147113

GI number: 162147113

Start: 1318393

End: 1321083

Strand: Direct

Name: podA [H]

Synonym: GDI_1318

Alternate gene names: 162147113

Gene position: 1318393-1321083 (Clockwise)

Preceding gene: 162147110

Following gene: 162147114

Centisome position: 33.43

GC content: 70.2

Gene sequence:

>2691_bases
ATGACGAAATGGGTCTATAGTTTCGGCGGTGCCGCCAACGAGGGCGGGGCCGGCATGCGCGACCTTCTGGGCGGCAAGGG
TGCCAACCTGGCCGAAATGGCGGCGATCGGCCTGCCCGTCCCGCCCGGCTTCACCATCACCACCGAGGTCTGCACCCGGT
TCTACGAAAACGGACGCGCCTATCCCGAAGACCTGCGCGCGCAGGTCGAAGCCGCGCTGGCCGGGGTCGAGGCGGCGCTG
GGCCTGCGCTTCGGCGATCCGGCGGCGCCCCTGCTGGTCTCGGTCCGCTCGGGCGCGCGGGTGTCGATGCCGGGCATGAT
GGATACCGTGCTGAATCTGGGCCTGAACGACGAAACGGTCGCCGGCCTGGCCGCCGCGTCGGGTGACGCGCGCTTCGCCT
GGGACAGCTATCGCCGGTTCATCCAGATGTACGGCTCGGTCGTGCTGGGCGTGTCCCATCATCGGTTCGAGGATCTGATC
GAGGAAACCAAGCGCGCGACCGGCGTGCAGGACGACACGAAGCTGACCGACCAGGACTGGCAGGAGGTCGTGACGGGCTA
CAAGGAGATCGTGCTGCGCGAAACCGGCGCACCGTTCCCCACGGACCCGCACGCCCAGTTGTGGGGCGCGATCGGCGCGG
TGTTCGGGTCCTGGATGAACCCGCGCGCCCATACCTATCGGCGGCTGCACGACATTCCCGCCGACTGGGGCACGGCGGTG
AACGTGCAGGCGATGGTGTTCGGCAACATGGGCGCGGACTGCGCGACCGGCGTCTGCTTCACCCGCGATCCCTCGACGGG
CGAAAACGTCTTCTACGGCGAATACCTGATCAACGCGCAGGGCGAGGACGTGGTGGCGGGCATCCGCACGCCGCAGCCGA
TGTCCCAGGCGCGCGCGCATGACGGCGAACTGTCGATGGAACGCGCGCTGCCCGAGGCGTACCGCGAATTGCTGGCGGTG
CGCGATATCCTGGAACGCCATTACCGCGACATGCAGGACATCGAATTCACCGTTCAGAACAACCGCCTGTACATGCTCCA
GACCCGCACCGGCAAGCGGACGGCCGCCGCCGCCCTGCGCATCGCCATCGCGATGGCCGAGGAAGGCCTGATTACGCACG
AGGAAGCGATCCGCCGCGTCCCGCCCTCGTCGCTCGACCAGCTGCTGCATCCGACGCTGGACCCGGACGCGCCGCGCACC
ATGCTCAGCCGGGGGCTGCCGGCCTCGCCCGGGGCGGCGGCGGGCGCCATCGTGTTCAGCGCCGAGGACGTGGAAATCCG
CGCCGCGCGGGGCGAGGACGTCATCCTGGTCCGTATCGAGACGTCGCCCGAGGACGTGCACGGCATGCACGCCGCGCGCG
GCGTGCTGACCACGCGCGGCGGCATGACCTCGCACGCCGCCGTGGTGGCGCGCGGCATGGGGCGGGTCTGCGTGGCCGGT
GCCGGCGGAATCCATGTCGATTACCGGGCGCGGACGCTGTCGGTCGGTGCGACGGTGCTGCATGAAGGCGACTGGATCAC
CCTGGACGGCGCGACGGGCGAGGTCTTCGCCGGCCGGGTCGCGACCATCGAGCCGACTCTGTCGGGCGATTTCTCGACCC
TGATGGGCTGGGCGGACAGCGTGCGCCGGATGAAGGTCCGCGCCAACGCCGAAACGCCCGACGATGCGCGGACCGCCCGG
CATTTCGGCGCCGAGGGGATCGGCCTGTGCCGGACCGAGCACATGTTCTTCGCCCCCGACCGCATCGGCTTCATGCGGCA
GATGATCATGGCCGGCGACGCGGCCGCGCGCATCCGGGCGATCGACGCCCTGCTGCCGTTCCAGCGCGAGGATTTCAAGG
CGCTGTTCCGCATCATGGCGGGCCTGCCGGTGACGATCCGCCTGCTGGACCCGCCGCTGCACGAATTCCTGCCGCATGGC
GAGGCCGAACTGGCCGAACTGGCCGCCGCGCTGGGCGAAAGCCCCGAGGCCGTGGCGGCGCGCCGGGCCGCGCTGTCGGA
AGCCAACCCGATGCTGGGCCATCGCGGCTGCCGGCTGGGCCTGACCTATCCGGAACTCTACGCCATGCAGGCCCGCGCGA
TCATCGAGGCCGCGATCGCCGTGTCCGCCGAAAGCGGGCAGCCGGTGGTTCCGGAAATCATGATTCCGCTGGTGGGGATG
GACAGCGAACTGCGCCGCACCCGCGCCACCGTCGAACAGGCCGCGGCCGAAGCCCTGGAAGCGGCCGGCGTGTCGCTGGA
TTTCAGCATCGGCACGATGATCGAACTGCCCCGCGCCGCGCTGACGGCCGACCGCATCGCGCAGGACGCCGATTTCTTCT
CGTTCGGCACCAACGACCTGACGCAGACGACCTTCGGCCTGTCGCGCGACGATTCCGGCACCTTCATGCCCTTCTACCTG
GAACATGACCTGATCCCGCGCGATCCGTTCATCTCGATCGACCAGGAGGGCGTGGGCCAGCTCGTCGCCATGGGGGCGGA
ACGGGGCCGCCGCACCCGGCCGGGGCTGAAGCTCGGGGTCTGCGGCGAACATGGCGGCGATCCGGCCTCGATCGGCTTCT
TCGAATCCGTGGGGCTGGATTACGTGTCGTGCTCGCCCTTCCGGGTGCCGATCGCGCGCCTGGCGGCGGCCCAGGCCGCG
CTGGCGGGCCGCGCCGACCCTGTCGCGTCCTCCCGGGTCGCGTCGTCCTAG

Upstream 100 bases:

>100_bases
AAAGTGGGGCGGAGTCCGGACAAGCAATCGAAAACAACAGTCCGGGACCAGGGGAAAGTCCACGCTTCACGCAGAATGGG
GCGGGGCGAAGGAGATACCG

Downstream 100 bases:

>100_bases
AGCGTGTGATCGGGTTACCCGATCACAGACTCCAGGTTTTTGTCTTCAGAGCAGCTTCACGTGATCAGGTGGTTCCACCT
GATCACGATCTGCTCCAGGC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 896; Mature: 895

Protein sequence:

>896_residues
MTKWVYSFGGAANEGGAGMRDLLGGKGANLAEMAAIGLPVPPGFTITTEVCTRFYENGRAYPEDLRAQVEAALAGVEAAL
GLRFGDPAAPLLVSVRSGARVSMPGMMDTVLNLGLNDETVAGLAAASGDARFAWDSYRRFIQMYGSVVLGVSHHRFEDLI
EETKRATGVQDDTKLTDQDWQEVVTGYKEIVLRETGAPFPTDPHAQLWGAIGAVFGSWMNPRAHTYRRLHDIPADWGTAV
NVQAMVFGNMGADCATGVCFTRDPSTGENVFYGEYLINAQGEDVVAGIRTPQPMSQARAHDGELSMERALPEAYRELLAV
RDILERHYRDMQDIEFTVQNNRLYMLQTRTGKRTAAAALRIAIAMAEEGLITHEEAIRRVPPSSLDQLLHPTLDPDAPRT
MLSRGLPASPGAAAGAIVFSAEDVEIRAARGEDVILVRIETSPEDVHGMHAARGVLTTRGGMTSHAAVVARGMGRVCVAG
AGGIHVDYRARTLSVGATVLHEGDWITLDGATGEVFAGRVATIEPTLSGDFSTLMGWADSVRRMKVRANAETPDDARTAR
HFGAEGIGLCRTEHMFFAPDRIGFMRQMIMAGDAAARIRAIDALLPFQREDFKALFRIMAGLPVTIRLLDPPLHEFLPHG
EAELAELAAALGESPEAVAARRAALSEANPMLGHRGCRLGLTYPELYAMQARAIIEAAIAVSAESGQPVVPEIMIPLVGM
DSELRRTRATVEQAAAEALEAAGVSLDFSIGTMIELPRAALTADRIAQDADFFSFGTNDLTQTTFGLSRDDSGTFMPFYL
EHDLIPRDPFISIDQEGVGQLVAMGAERGRRTRPGLKLGVCGEHGGDPASIGFFESVGLDYVSCSPFRVPIARLAAAQAA
LAGRADPVASSRVASS

Sequences:

>Translated_896_residues
MTKWVYSFGGAANEGGAGMRDLLGGKGANLAEMAAIGLPVPPGFTITTEVCTRFYENGRAYPEDLRAQVEAALAGVEAAL
GLRFGDPAAPLLVSVRSGARVSMPGMMDTVLNLGLNDETVAGLAAASGDARFAWDSYRRFIQMYGSVVLGVSHHRFEDLI
EETKRATGVQDDTKLTDQDWQEVVTGYKEIVLRETGAPFPTDPHAQLWGAIGAVFGSWMNPRAHTYRRLHDIPADWGTAV
NVQAMVFGNMGADCATGVCFTRDPSTGENVFYGEYLINAQGEDVVAGIRTPQPMSQARAHDGELSMERALPEAYRELLAV
RDILERHYRDMQDIEFTVQNNRLYMLQTRTGKRTAAAALRIAIAMAEEGLITHEEAIRRVPPSSLDQLLHPTLDPDAPRT
MLSRGLPASPGAAAGAIVFSAEDVEIRAARGEDVILVRIETSPEDVHGMHAARGVLTTRGGMTSHAAVVARGMGRVCVAG
AGGIHVDYRARTLSVGATVLHEGDWITLDGATGEVFAGRVATIEPTLSGDFSTLMGWADSVRRMKVRANAETPDDARTAR
HFGAEGIGLCRTEHMFFAPDRIGFMRQMIMAGDAAARIRAIDALLPFQREDFKALFRIMAGLPVTIRLLDPPLHEFLPHG
EAELAELAAALGESPEAVAARRAALSEANPMLGHRGCRLGLTYPELYAMQARAIIEAAIAVSAESGQPVVPEIMIPLVGM
DSELRRTRATVEQAAAEALEAAGVSLDFSIGTMIELPRAALTADRIAQDADFFSFGTNDLTQTTFGLSRDDSGTFMPFYL
EHDLIPRDPFISIDQEGVGQLVAMGAERGRRTRPGLKLGVCGEHGGDPASIGFFESVGLDYVSCSPFRVPIARLAAAQAA
LAGRADPVASSRVASS
>Mature_895_residues
TKWVYSFGGAANEGGAGMRDLLGGKGANLAEMAAIGLPVPPGFTITTEVCTRFYENGRAYPEDLRAQVEAALAGVEAALG
LRFGDPAAPLLVSVRSGARVSMPGMMDTVLNLGLNDETVAGLAAASGDARFAWDSYRRFIQMYGSVVLGVSHHRFEDLIE
ETKRATGVQDDTKLTDQDWQEVVTGYKEIVLRETGAPFPTDPHAQLWGAIGAVFGSWMNPRAHTYRRLHDIPADWGTAVN
VQAMVFGNMGADCATGVCFTRDPSTGENVFYGEYLINAQGEDVVAGIRTPQPMSQARAHDGELSMERALPEAYRELLAVR
DILERHYRDMQDIEFTVQNNRLYMLQTRTGKRTAAAALRIAIAMAEEGLITHEEAIRRVPPSSLDQLLHPTLDPDAPRTM
LSRGLPASPGAAAGAIVFSAEDVEIRAARGEDVILVRIETSPEDVHGMHAARGVLTTRGGMTSHAAVVARGMGRVCVAGA
GGIHVDYRARTLSVGATVLHEGDWITLDGATGEVFAGRVATIEPTLSGDFSTLMGWADSVRRMKVRANAETPDDARTARH
FGAEGIGLCRTEHMFFAPDRIGFMRQMIMAGDAAARIRAIDALLPFQREDFKALFRIMAGLPVTIRLLDPPLHEFLPHGE
AELAELAAALGESPEAVAARRAALSEANPMLGHRGCRLGLTYPELYAMQARAIIEAAIAVSAESGQPVVPEIMIPLVGMD
SELRRTRATVEQAAAEALEAAGVSLDFSIGTMIELPRAALTADRIAQDADFFSFGTNDLTQTTFGLSRDDSGTFMPFYLE
HDLIPRDPFISIDQEGVGQLVAMGAERGRRTRPGLKLGVCGEHGGDPASIGFFESVGLDYVSCSPFRVPIARLAAAQAAL
AGRADPVASSRVASS

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=463, Percent_Identity=25.4859611231102, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1789193, Length=426, Percent_Identity=26.2910798122066, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1787994, Length=714, Percent_Identity=25.3501400560224, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788726, Length=447, Percent_Identity=24.3847874720358, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI48994992, Length=215, Percent_Identity=27.906976744186, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 96183; Mature: 96052

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWVYSFGGAANEGGAGMRDLLGGKGANLAEMAAIGLPVPPGFTITTEVCTRFYENGRA
CCCEEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCC
YPEDLRAQVEAALAGVEAALGLRFGDPAAPLLVSVRSGARVSMPGMMDTVLNLGLNDETV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEECCHHHHHHHHCCCCCHHH
AGLAAASGDARFAWDSYRRFIQMYGSVVLGVSHHRFEDLIEETKRATGVQDDTKLTDQDW
HHEEECCCCCCCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
QEVVTGYKEIVLRETGAPFPTDPHAQLWGAIGAVFGSWMNPRAHTYRRLHDIPADWGTAV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEE
NVQAMVFGNMGADCATGVCFTRDPSTGENVFYGEYLINAQGEDVVAGIRTPQPMSQARAH
EEEEEEECCCCCHHHCCEEEECCCCCCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHCC
DGELSMERALPEAYRELLAVRDILERHYRDMQDIEFTVQNNRLYMLQTRTGKRTAAAALR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCEEEEEEECCCCHHHHHHHH
IAIAMAEEGLITHEEAIRRVPPSSLDQLLHPTLDPDAPRTMLSRGLPASPGAAAGAIVFS
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEE
AEDVEIRAARGEDVILVRIETSPEDVHGMHAARGVLTTRGGMTSHAAVVARGMGRVCVAG
CCCCEEEEECCCCEEEEEEECCCCHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCEEEEC
AGGIHVDYRARTLSVGATVLHEGDWITLDGATGEVFAGRVATIEPTLSGDFSTLMGWADS
CCCEEEEEEEEEEEECEEEEECCCEEEEECCCCCEEECEEEEECCCCCCCHHHHHHHHHH
VRRMKVRANAETPDDARTARHFGAEGIGLCRTEHMFFAPDRIGFMRQMIMAGDAAARIRA
HHHEEEEECCCCCCHHHHHHHCCCCCCCEEEECCEEECCHHHHHHHHHHHHCCHHHHHHH
IDALLPFQREDFKALFRIMAGLPVTIRLLDPPLHEFLPHGEAELAELAAALGESPEAVAA
HHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHHHHH
RRAALSEANPMLGHRGCRLGLTYPELYAMQARAIIEAAIAVSAESGQPVVPEIMIPLVGM
HHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCC
DSELRRTRATVEQAAAEALEAAGVSLDFSIGTMIELPRAALTADRIAQDADFFSFGTNDL
CHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCHHHHHHHHHHHCCCHHCCCCCCH
TQTTFGLSRDDSGTFMPFYLEHDLIPRDPFISIDQEGVGQLVAMGAERGRRTRPGLKLGV
HHHHCCCCCCCCCCEEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCEEEE
CGEHGGDPASIGFFESVGLDYVSCSPFRVPIARLAAAQAALAGRADPVASSRVASS
CCCCCCCCCCCCHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure 
TKWVYSFGGAANEGGAGMRDLLGGKGANLAEMAAIGLPVPPGFTITTEVCTRFYENGRA
CCEEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCC
YPEDLRAQVEAALAGVEAALGLRFGDPAAPLLVSVRSGARVSMPGMMDTVLNLGLNDETV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEECCHHHHHHHHCCCCCHHH
AGLAAASGDARFAWDSYRRFIQMYGSVVLGVSHHRFEDLIEETKRATGVQDDTKLTDQDW
HHEEECCCCCCCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
QEVVTGYKEIVLRETGAPFPTDPHAQLWGAIGAVFGSWMNPRAHTYRRLHDIPADWGTAV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEE
NVQAMVFGNMGADCATGVCFTRDPSTGENVFYGEYLINAQGEDVVAGIRTPQPMSQARAH
EEEEEEECCCCCHHHCCEEEECCCCCCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHCC
DGELSMERALPEAYRELLAVRDILERHYRDMQDIEFTVQNNRLYMLQTRTGKRTAAAALR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCEEEEEEECCCCHHHHHHHH
IAIAMAEEGLITHEEAIRRVPPSSLDQLLHPTLDPDAPRTMLSRGLPASPGAAAGAIVFS
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEE
AEDVEIRAARGEDVILVRIETSPEDVHGMHAARGVLTTRGGMTSHAAVVARGMGRVCVAG
CCCCEEEEECCCCEEEEEEECCCCHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCEEEEC
AGGIHVDYRARTLSVGATVLHEGDWITLDGATGEVFAGRVATIEPTLSGDFSTLMGWADS
CCCEEEEEEEEEEEECEEEEECCCEEEEECCCCCEEECEEEEECCCCCCCHHHHHHHHHH
VRRMKVRANAETPDDARTARHFGAEGIGLCRTEHMFFAPDRIGFMRQMIMAGDAAARIRA
HHHEEEEECCCCCCHHHHHHHCCCCCCCEEEECCEEECCHHHHHHHHHHHHCCHHHHHHH
IDALLPFQREDFKALFRIMAGLPVTIRLLDPPLHEFLPHGEAELAELAAALGESPEAVAA
HHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHHHHH
RRAALSEANPMLGHRGCRLGLTYPELYAMQARAIIEAAIAVSAESGQPVVPEIMIPLVGM
HHHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCC
DSELRRTRATVEQAAAEALEAAGVSLDFSIGTMIELPRAALTADRIAQDADFFSFGTNDL
CHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCHHHHHHHHHHHCCCHHCCCCCCH
TQTTFGLSRDDSGTFMPFYLEHDLIPRDPFISIDQEGVGQLVAMGAERGRRTRPGLKLGV
HHHHCCCCCCCCCCEEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCEEEE
CGEHGGDPASIGFFESVGLDYVSCSPFRVPIARLAAAQAALAGRADPVASSRVASS
CCCCCCCCCCCCHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]