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The map label for this gene is rluB [C]

Identifier: 162147095

GI number: 162147095

Start: 1299781

End: 1300575

Strand: Reverse

Name: rluB [C]

Synonym: GDI_1300

Alternate gene names: 162147095

Gene position: 1300575-1299781 (Counterclockwise)

Preceding gene: 162147096

Following gene: 162147094

Centisome position: 32.97

GC content: 72.45

Gene sequence:

>795_bases
ATGACACAGCACGACGACGACACGACCCAACCGGGGCACCCCGACCGGCCCGAACGTGGCGAGCGCATCGCCAAATGGCT
GGCGCGGTCCGGCGTCGCCAGCCGCCGCGACGCCGAGCGCCTGGTCGAGGAAGGGCGCGTGCGCCTGAACGGCGAACCCG
TCACCCATCCGGCGACCTTCGTCGCGGCGGGCGACATCGTGCAGGTGAACGGCGCGGTGGTCGAGGCGCCCGAACGGACC
CGGCTCTGGCGCTATCACAAGCCGGACGGGCTGGTGACGACGCACCGCGACCCCGAGAAGCGGCCGACCGTGTTCGCCTC
GCTGCCGCCGGGCATGCCGCGCGTCATCAGCGTCGGGCGGCTGGACCTGAACAGCGAGGGGCTGCTGCTGCTGACCAATG
ACGGCGAACTGGCCCGGCGGCTGGAACTGCCGTCGAACGGCTGGCTGCGCCGCTATCGCGTGCGCGTCTTCGGCGTGGTG
GACGAGGCGCGGCTGGCGGCCCTGGCCGGGGGCAGCGTGTTCGAGGGCGTGCGTTACGGGCCGATCGAGGCCGGGCTGGA
TTCCCGCAAGGGCGACAATGCCTGGCTGACCGTGGCGCTGCGCGAGGGCAAGAATCGCGAGGTGCGCAAGGTCATGCGCG
GGCTGAACCTGCATGTCAGCCGGCTGATCCGCACATCCTACGGTCCGTTCCAGCTCGGCACGCTGCAGCGCTCGGAACTG
GAGGAAGTGCCGGGCAAGGTCCTGCGCGAGCAGGTGCCCGGACTGGGCCCGGCGCCCCGGCCCGCCCGCCGGTAG

Upstream 100 bases:

>100_bases
CACGGCGGTTTTGCCTGGGCGTGCAATGGCACCCCGAATTCGCCATATCGGTAGCCGATAGACGCATCTTTGCCGCCTTG
ATAGAGACCTCGGGATCGAG

Downstream 100 bases:

>100_bases
GCCGGATGCGCATCGTCGCCGGAACGTGGCGCGGCCGCGCCCTGGCCGCGCCCGCCGGGCGCACCACCCGCCCGACGGCG
GACCGCGTTCGCCAGGCACT

Product: ribosomal large subunit pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTQHDDDTTQPGHPDRPERGERIAKWLARSGVASRRDAERLVEEGRVRLNGEPVTHPATFVAAGDIVQVNGAVVEAPERT
RLWRYHKPDGLVTTHRDPEKRPTVFASLPPGMPRVISVGRLDLNSEGLLLLTNDGELARRLELPSNGWLRRYRVRVFGVV
DEARLAALAGGSVFEGVRYGPIEAGLDSRKGDNAWLTVALREGKNREVRKVMRGLNLHVSRLIRTSYGPFQLGTLQRSEL
EEVPGKVLREQVPGLGPAPRPARR

Sequences:

>Translated_264_residues
MTQHDDDTTQPGHPDRPERGERIAKWLARSGVASRRDAERLVEEGRVRLNGEPVTHPATFVAAGDIVQVNGAVVEAPERT
RLWRYHKPDGLVTTHRDPEKRPTVFASLPPGMPRVISVGRLDLNSEGLLLLTNDGELARRLELPSNGWLRRYRVRVFGVV
DEARLAALAGGSVFEGVRYGPIEAGLDSRKGDNAWLTVALREGKNREVRKVMRGLNLHVSRLIRTSYGPFQLGTLQRSEL
EEVPGKVLREQVPGLGPAPRPARR
>Mature_263_residues
TQHDDDTTQPGHPDRPERGERIAKWLARSGVASRRDAERLVEEGRVRLNGEPVTHPATFVAAGDIVQVNGAVVEAPERTR
LWRYHKPDGLVTTHRDPEKRPTVFASLPPGMPRVISVGRLDLNSEGLLLLTNDGELARRLELPSNGWLRRYRVRVFGVVD
EARLAALAGGSVFEGVRYGPIEAGLDSRKGDNAWLTVALREGKNREVRKVMRGLNLHVSRLIRTSYGPFQLGTLQRSELE
EVPGKVLREQVPGLGPAPRPARR

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=218, Percent_Identity=42.2018348623853, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790453, Length=211, Percent_Identity=30.8056872037915, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87081838, Length=179, Percent_Identity=31.2849162011173, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788510, Length=212, Percent_Identity=26.8867924528302, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 29379; Mature: 29248

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHDDDTTQPGHPDRPERGERIAKWLARSGVASRRDAERLVEEGRVRLNGEPVTHPATF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCEEE
VAAGDIVQVNGAVVEAPERTRLWRYHKPDGLVTTHRDPEKRPTVFASLPPGMPRVISVGR
EEECCEEEECCEEEECCCHHCCEEEECCCCCEECCCCCCCCCEEEEECCCCCHHHEEECE
LDLNSEGLLLLTNDGELARRLELPSNGWLRRYRVRVFGVVDEARLAALAGGSVFEGVRYG
EEECCCCEEEEECCCHHHHEECCCCCCCCEEEEEEEEEEHHHHHHHHHCCCHHHCCCCCC
PIEAGLDSRKGDNAWLTVALREGKNREVRKVMRGLNLHVSRLIRTSYGPFQLGTLQRSEL
CHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHH
EEVPGKVLREQVPGLGPAPRPARR
HHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TQHDDDTTQPGHPDRPERGERIAKWLARSGVASRRDAERLVEEGRVRLNGEPVTHPATF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCEEE
VAAGDIVQVNGAVVEAPERTRLWRYHKPDGLVTTHRDPEKRPTVFASLPPGMPRVISVGR
EEECCEEEECCEEEECCCHHCCEEEECCCCCEECCCCCCCCCEEEEECCCCCHHHEEECE
LDLNSEGLLLLTNDGELARRLELPSNGWLRRYRVRVFGVVDEARLAALAGGSVFEGVRYG
EEECCCCEEEEECCCHHHHEECCCCCCCCEEEEEEEEEEHHHHHHHHHCCCHHHCCCCCC
PIEAGLDSRKGDNAWLTVALREGKNREVRKVMRGLNLHVSRLIRTSYGPFQLGTLQRSEL
CHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHH
EEVPGKVLREQVPGLGPAPRPARR
HHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA