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The map label for this gene is pykA [H]

Identifier: 162147085

GI number: 162147085

Start: 1289801

End: 1291240

Strand: Reverse

Name: pykA [H]

Synonym: GDI_1290

Alternate gene names: 162147085

Gene position: 1291240-1289801 (Counterclockwise)

Preceding gene: 162147086

Following gene: 162147078

Centisome position: 32.74

GC content: 70.35

Gene sequence:

>1440_bases
ATGACCATCGTCTCCGCCTCTCGTCCCCGTCGGACGAAAATCGTCTCGACCCTCGGCCCGGCATCGTCCAGCCGGGAAGT
GATCCGCGCACTGGCCGAGGCCGGCGCCGACGTGTTCCGCCTGAACTTCTCGCACGGCACGCATGCGGACCACGCCGCGC
GGCATGAGATCATCCGCTCGGTCGAGGCCGAGCTGGGCCGCCCGATCGGCATCCTGGCCGACGTCCAGGGCCCCAAGCTG
CGCGTGGGCACCTTCGAGAACGGCAAGGTGACGCTGCGGACCGGCGCCTCGTTCCGCCTGGACCTGGACGACGCGCCCGG
CAACGAGGAACGCGTGCGCCTGCCGCACCCGGAAATCATCCATGCCGCGACGGTCGGCAGCAACCTGCTGCTGGATGACG
GCAAGCTGCGGCTGGTGGTGCGTGAGGTCGGACCCACCTACCTGGATACGATCGTGGCCGTGGGCGGCGTGCTGTCCGAG
CGCAAGGGCGTGAACGTGCCCGACATCGTGCTGCCCATCCCCGCCCTGACCCCGAAGGACCGCAAGGACCTGGATTTCGC
GCTGGAACTGGGCGTCGAATATATCGGCCTGTCCTTCGTCCAGCGCCCCGAGGACGTCGAGGAAGCCCGCGCCATCGCCG
CCGGCCGCGCCTGGATCATGACCAAGGTGGAAAAACCGCAGGCCATGGACAATCTGGACGAGATCGTCCGCCTGTCCGAC
GCGGTGATGGTCGCGCGCGGCGACCTGGGCGTCGAACTGCCGCCCGAGGCCGTGCCGCTGGCCCAGAAGCGCATCATCCG
CCTGGCCCGCCAGTTGGGCAAGCCGGTGGTGGTCGCGACCCAGATGCTGGAAAGCATGATCACCGCCCCGACCCCCACCC
GGGCCGAGGCGTCGGACGTGGCCACCGCCGTGTTCGACGGCGCCGACGCCGTGATGCTGTCGGCCGAAACCGCGGCCGGC
CAGTATCCGCAGGAAGCGGTCGGCATCATGAACCGGATCGTCAGCCGCGTCGAAGAGGACTCGGTCTGGCAGTCGATCAT
GCAGGCAAGCTGCCCGGCGCCCGAGGGATTCGTCGCCGATGCGATCGCGGCGGCGGCGGGGCAGGTCGCCACCACCGTGG
GCGCGCGCGCGATCGTCGCCTTCACCCTGGCCGGCGCCACCGCGCTGCGCATCTCGCGCGAGCGGCCGTCGTGCCCCATC
ATCGGCCTGACGCCCACCGACGAGATCGCGCGGCGGCTGTGCGTCGTCTGGGGGGTCCAGGCGCAGTCCGTCGGACAGGA
AACGCCGGTGCGCACCGTCGAAACCGTGGTGGACCAGGCGATCGAATCCACGGTCGCCAGCAGCCTGGCACGGTCGGGCG
ATACGATCGTGATCGCGGCCGGGCTGCCGTTCGGCGTGGCGGGCAGCACCAACACGCTGCGGGTCGCCCGGGTGAAGTAA

Upstream 100 bases:

>100_bases
GCAGGAGACCATCCAGCCGTAGTGACGCCGATCGGGCGTCCGACTGACGAAGACCAGAAGATCCGCTTACGGACATGAAT
CCAGAAGGAGAAAGCGCCCT

Downstream 100 bases:

>100_bases
ACGTTCGGGGCCCTGTCCCGGACTCCGCCTAGACGTTGCCGGGACCGGCGTTTTCCAGCCGGTCCAGCAACGGATGATCC
TCCAGGTACAACAGGAAACC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTIVSASRPRRTKIVSTLGPASSSREVIRALAEAGADVFRLNFSHGTHADHAARHEIIRSVEAELGRPIGILADVQGPKL
RVGTFENGKVTLRTGASFRLDLDDAPGNEERVRLPHPEIIHAATVGSNLLLDDGKLRLVVREVGPTYLDTIVAVGGVLSE
RKGVNVPDIVLPIPALTPKDRKDLDFALELGVEYIGLSFVQRPEDVEEARAIAAGRAWIMTKVEKPQAMDNLDEIVRLSD
AVMVARGDLGVELPPEAVPLAQKRIIRLARQLGKPVVVATQMLESMITAPTPTRAEASDVATAVFDGADAVMLSAETAAG
QYPQEAVGIMNRIVSRVEEDSVWQSIMQASCPAPEGFVADAIAAAAGQVATTVGARAIVAFTLAGATALRISRERPSCPI
IGLTPTDEIARRLCVVWGVQAQSVGQETPVRTVETVVDQAIESTVASSLARSGDTIVIAAGLPFGVAGSTNTLRVARVK

Sequences:

>Translated_479_residues
MTIVSASRPRRTKIVSTLGPASSSREVIRALAEAGADVFRLNFSHGTHADHAARHEIIRSVEAELGRPIGILADVQGPKL
RVGTFENGKVTLRTGASFRLDLDDAPGNEERVRLPHPEIIHAATVGSNLLLDDGKLRLVVREVGPTYLDTIVAVGGVLSE
RKGVNVPDIVLPIPALTPKDRKDLDFALELGVEYIGLSFVQRPEDVEEARAIAAGRAWIMTKVEKPQAMDNLDEIVRLSD
AVMVARGDLGVELPPEAVPLAQKRIIRLARQLGKPVVVATQMLESMITAPTPTRAEASDVATAVFDGADAVMLSAETAAG
QYPQEAVGIMNRIVSRVEEDSVWQSIMQASCPAPEGFVADAIAAAAGQVATTVGARAIVAFTLAGATALRISRERPSCPI
IGLTPTDEIARRLCVVWGVQAQSVGQETPVRTVETVVDQAIESTVASSLARSGDTIVIAAGLPFGVAGSTNTLRVARVK
>Mature_478_residues
TIVSASRPRRTKIVSTLGPASSSREVIRALAEAGADVFRLNFSHGTHADHAARHEIIRSVEAELGRPIGILADVQGPKLR
VGTFENGKVTLRTGASFRLDLDDAPGNEERVRLPHPEIIHAATVGSNLLLDDGKLRLVVREVGPTYLDTIVAVGGVLSER
KGVNVPDIVLPIPALTPKDRKDLDFALELGVEYIGLSFVQRPEDVEEARAIAAGRAWIMTKVEKPQAMDNLDEIVRLSDA
VMVARGDLGVELPPEAVPLAQKRIIRLARQLGKPVVVATQMLESMITAPTPTRAEASDVATAVFDGADAVMLSAETAAGQ
YPQEAVGIMNRIVSRVEEDSVWQSIMQASCPAPEGFVADAIAAAAGQVATTVGARAIVAFTLAGATALRISRERPSCPII
GLTPTDEIARRLCVVWGVQAQSVGQETPVRTVETVVDQAIESTVASSLARSGDTIVIAAGLPFGVAGSTNTLRVARVK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=37.0901639344262, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=37.0901639344262, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=37.3716632443532, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=39.0593047034765, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=39.0593047034765, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=37.6306620209059, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=37.6306620209059, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=34.0425531914894, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=34.0425531914894, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=32.7102803738318, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1788160, Length=490, Percent_Identity=43.0612244897959, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1787965, Length=474, Percent_Identity=37.5527426160338, Blast_Score=298, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=37.8099173553719, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=38.0165289256198, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=38.0165289256198, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=38.0165289256198, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=38.0165289256198, Blast_Score=273, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319279, Length=494, Percent_Identity=35.6275303643725, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=496, Percent_Identity=35.2822580645161, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=37.5510204081633, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=37.5510204081633, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24648966, Length=387, Percent_Identity=40.0516795865633, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=33.5378323108384, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24646914, Length=249, Percent_Identity=40.5622489959839, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50779; Mature: 50648

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVSASRPRRTKIVSTLGPASSSREVIRALAEAGADVFRLNFSHGTHADHAARHEIIRS
CCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
VEAELGRPIGILADVQGPKLRVGTFENGKVTLRTGASFRLDLDDAPGNEERVRLPHPEII
HHHHHCCCEEEEEECCCCEEEEEECCCCEEEEEECCEEEEECCCCCCCCCEEECCCCCEE
HAATVGSNLLLDDGKLRLVVREVGPTYLDTIVAVGGVLSERKGVNVPDIVLPIPALTPKD
EEECCCCCEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCC
RKDLDFALELGVEYIGLSFVQRPEDVEEARAIAAGRAWIMTKVEKPQAMDNLDEIVRLSD
CCCHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHH
AVMVARGDLGVELPPEAVPLAQKRIIRLARQLGKPVVVATQMLESMITAPTPTRAEASDV
HEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
ATAVFDGADAVMLSAETAAGQYPQEAVGIMNRIVSRVEEDSVWQSIMQASCPAPEGFVAD
HHHHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AIAAAAGQVATTVGARAIVAFTLAGATALRISRERPSCPIIGLTPTDEIARRLCVVWGVQ
HHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCEEECCCHHHHHHHHHHHHCCC
AQSVGQETPVRTVETVVDQAIESTVASSLARSGDTIVIAAGLPFGVAGSTNTLRVARVK
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure 
TIVSASRPRRTKIVSTLGPASSSREVIRALAEAGADVFRLNFSHGTHADHAARHEIIRS
CCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
VEAELGRPIGILADVQGPKLRVGTFENGKVTLRTGASFRLDLDDAPGNEERVRLPHPEII
HHHHHCCCEEEEEECCCCEEEEEECCCCEEEEEECCEEEEECCCCCCCCCEEECCCCCEE
HAATVGSNLLLDDGKLRLVVREVGPTYLDTIVAVGGVLSERKGVNVPDIVLPIPALTPKD
EEECCCCCEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCC
RKDLDFALELGVEYIGLSFVQRPEDVEEARAIAAGRAWIMTKVEKPQAMDNLDEIVRLSD
CCCHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHH
AVMVARGDLGVELPPEAVPLAQKRIIRLARQLGKPVVVATQMLESMITAPTPTRAEASDV
HEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
ATAVFDGADAVMLSAETAAGQYPQEAVGIMNRIVSRVEEDSVWQSIMQASCPAPEGFVAD
HHHHHCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AIAAAAGQVATTVGARAIVAFTLAGATALRISRERPSCPIIGLTPTDEIARRLCVVWGVQ
HHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCEEECCCHHHHHHHHHHHHCCC
AQSVGQETPVRTVETVVDQAIESTVASSLARSGDTIVIAAGLPFGVAGSTNTLRVARVK
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA