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The map label for this gene is trmB [H]

Identifier: 162147053

GI number: 162147053

Start: 1253450

End: 1254175

Strand: Reverse

Name: trmB [H]

Synonym: GDI_1258

Alternate gene names: 162147053

Gene position: 1254175-1253450 (Counterclockwise)

Preceding gene: 162147054

Following gene: 162147050

Centisome position: 31.8

GC content: 68.87

Gene sequence:

>726_bases
ATGACGGTCCCGCAGGCACCTGACGGTACGGTATCGGGGGTCAAGGCTTCGCCTGACCGGCTGTATGGCCGTCAGCGCGG
CCATCCGCTGCGCCCGCGCCAGCAGCGCCTGCTGGACCTGACGCTGCCGCGACTGCGGCTGGACCCGTCGGTTCTGGATG
ACCCGGCACGCGGTTTCGGCCGCGTGCCGTCCGAAATCTGGCTGGAGGTCGGGTTCGGCGGTGGCGAGCATTCGCTGGCC
CAGCACGCGGCCCATCCCGACGTGGGTTATATCGCCAGCGAAGTCTTCGAAAACGGGCTGTGCTCGCTGCTGTCGCGCCT
GGTGCCCGACGGGCAGGAGGGTACCGCCCCGGTGCCCGACCTGCTGCGGATATGGGACGAGGATGCGCGCATCCTGCTGC
GCGCCCTGCCCGAACGGTCGATCGACCGGATGTTCCTGATGTTTCCCGACCCGTGGCCCAAGGCCCGGCATGCCAAGCGC
CGCTTCGTCCATCCCGCGACGATCGCGCTGGTCGCCCGCGTGTTGAAGCCCGGCGCCATCTGGCGTGTTGCCAGCGACGA
CCCGACCTATCAGACCTGGGTGAGCGAGGTGATGGGCGCGCAGGATCTGTTCGCCACCGCACCGCCCGCCACCGTCCGGC
CCGAAGGGTGGCCGCCGACGCGCTATGAGGCCAAGGCGCTGCTTGCGGGGCGGCAGCCTTTGTATTGGACGTTTGTAAGG
AAGTAA

Upstream 100 bases:

>100_bases
ACGCCGGATGAAGCCCGCGACAATTTCACGTGGGAGAAGACCGACCTGGTGGACGCGCTGCGCGGCGCGTTCAACCGCTG
ATCGCGGCAGCCAAGGTCGT

Downstream 100 bases:

>100_bases
GAGTCTTCTTTTTCTGAAGAAAAAGAAGCAAAAAGACTTTTATTCGTTTGGTCCGCCGTATGTACTCGGCACAAAATTTC
TGAATAGATCAGAAGTTTTT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTVPQAPDGTVSGVKASPDRLYGRQRGHPLRPRQQRLLDLTLPRLRLDPSVLDDPARGFGRVPSEIWLEVGFGGGEHSLA
QHAAHPDVGYIASEVFENGLCSLLSRLVPDGQEGTAPVPDLLRIWDEDARILLRALPERSIDRMFLMFPDPWPKARHAKR
RFVHPATIALVARVLKPGAIWRVASDDPTYQTWVSEVMGAQDLFATAPPATVRPEGWPPTRYEAKALLAGRQPLYWTFVR
K

Sequences:

>Translated_241_residues
MTVPQAPDGTVSGVKASPDRLYGRQRGHPLRPRQQRLLDLTLPRLRLDPSVLDDPARGFGRVPSEIWLEVGFGGGEHSLA
QHAAHPDVGYIASEVFENGLCSLLSRLVPDGQEGTAPVPDLLRIWDEDARILLRALPERSIDRMFLMFPDPWPKARHAKR
RFVHPATIALVARVLKPGAIWRVASDDPTYQTWVSEVMGAQDLFATAPPATVRPEGWPPTRYEAKALLAGRQPLYWTFVR
K
>Mature_240_residues
TVPQAPDGTVSGVKASPDRLYGRQRGHPLRPRQQRLLDLTLPRLRLDPSVLDDPARGFGRVPSEIWLEVGFGGGEHSLAQ
HAAHPDVGYIASEVFENGLCSLLSRLVPDGQEGTAPVPDLLRIWDEDARILLRALPERSIDRMFLMFPDPWPKARHAKRR
FVHPATIALVARVLKPGAIWRVASDDPTYQTWVSEVMGAQDLFATAPPATVRPEGWPPTRYEAKALLAGRQPLYWTFVRK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=213, Percent_Identity=30.5164319248826, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26928; Mature: 26797

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPQAPDGTVSGVKASPDRLYGRQRGHPLRPRQQRLLDLTLPRLRLDPSVLDDPARGFG
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCHHHCCCHHHHC
RVPSEIWLEVGFGGGEHSLAQHAAHPDVGYIASEVFENGLCSLLSRLVPDGQEGTAPVPD
CCCHHHEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LLRIWDEDARILLRALPERSIDRMFLMFPDPWPKARHAKRRFVHPATIALVARVLKPGAI
HHHHHCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCE
WRVASDDPTYQTWVSEVMGAQDLFATAPPATVRPEGWPPTRYEAKALLAGRQPLYWTFVR
EEECCCCCHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEC
K
C
>Mature Secondary Structure 
TVPQAPDGTVSGVKASPDRLYGRQRGHPLRPRQQRLLDLTLPRLRLDPSVLDDPARGFG
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCHHHCCCHHHHC
RVPSEIWLEVGFGGGEHSLAQHAAHPDVGYIASEVFENGLCSLLSRLVPDGQEGTAPVPD
CCCHHHEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LLRIWDEDARILLRALPERSIDRMFLMFPDPWPKARHAKRRFVHPATIALVARVLKPGAI
HHHHHCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCE
WRVASDDPTYQTWVSEVMGAQDLFATAPPATVRPEGWPPTRYEAKALLAGRQPLYWTFVR
EEECCCCCHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA