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The map label for this gene is trpA

Identifier: 162147049

GI number: 162147049

Start: 1248560

End: 1249384

Strand: Reverse

Name: trpA

Synonym: GDI_1254

Alternate gene names: 162147049

Gene position: 1249384-1248560 (Counterclockwise)

Preceding gene: 162147050

Following gene: 162147048

Centisome position: 31.68

GC content: 73.94

Gene sequence:

>825_bases
GTGAGCCGCATCGCCCGCCGTTTCGCGGCCCTGAAGGCGCAGGACCGTGGCGCCCTGATTCCCTACCTGCAGGCCTGCGA
TCCGGATTACGAGACCTCGCTGGCGCTGCTGCGTGCCATGCCCGGGGCCGGGGCCGACCTGATCGAGATCGGCGTGCCCT
TCAGCGACCCCAGCGCCGACGGCCCCACCATCCAGGCCGCCGCCCGGCGCGGGCTGGCGGCCGGCGCCACCATGGCGCGC
GTGCTGGAGATGGTCACCCGCTTCCGCGAGGGCGATGCCGACACGCCGATCGTGCTGATGGGCTACCTGAACCCGATCGA
GGCCTATGGGCCCGCGCGCTTCTGCGCCGATGCCGCCCGGGCTGGGGTGGACGGGCTGATCGTCGTCGACCTGCCGCCCG
AGGAGGCCGACCTGCTGGAAGGCCCGGCGGCGGACCACGGGCTGGACATCATCCGGCTGGTGGCGCCCACCACCGACGAT
ACGCGCCTGCCGCTGGTCTGCCGCCATGCCTCGGGCTTCATCTATTACGTCAGCATCACCGGGGTGACTGGCACGCGCAC
CGCCAGCGCCGACGAACTGGCCCAGGCGCTGCCGCGCCTGCGGGCCGCGACCGACCTGCCGGTGGCCATCGGCTTCGGCA
TCCGCACGCCCGAACAGGCGGCGGCCGCCGTACGCGTCGCCGACGCGGCCGTGGTGGCGTCGGCGCTGATCGCCACGCTG
GAGGACTCGCTGGACGCCGAGGGCCGCGCCGGACCGGACACGCTGAAGCGCGTGCTCGCGCAGCTCGAAGCCCTGGGGCG
GGCGGTACGAAACGCCCGCACCTGA

Upstream 100 bases:

>100_bases
TCGCGCCCACCCTGGACCCGGAGACTCTGGTCGTCCTGAACGTCTCCGGCCGGGGGGACAAGGACATCTTCACCGTCGCA
GCACATCTGGGAGTGACATT

Downstream 100 bases:

>100_bases
ACCGAAGGCCCCCGCACAAGCTGGTTCTGGCATTTCCCGGCACCAGCTTGTAAGGAGGCAGGTTTCCGGGGCCGTCATGC
GCCCTGCGTCATGGAGAGAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSRIARRFAALKAQDRGALIPYLQACDPDYETSLALLRAMPGAGADLIEIGVPFSDPSADGPTIQAAARRGLAAGATMAR
VLEMVTRFREGDADTPIVLMGYLNPIEAYGPARFCADAARAGVDGLIVVDLPPEEADLLEGPAADHGLDIIRLVAPTTDD
TRLPLVCRHASGFIYYVSITGVTGTRTASADELAQALPRLRAATDLPVAIGFGIRTPEQAAAAVRVADAAVVASALIATL
EDSLDAEGRAGPDTLKRVLAQLEALGRAVRNART

Sequences:

>Translated_274_residues
MSRIARRFAALKAQDRGALIPYLQACDPDYETSLALLRAMPGAGADLIEIGVPFSDPSADGPTIQAAARRGLAAGATMAR
VLEMVTRFREGDADTPIVLMGYLNPIEAYGPARFCADAARAGVDGLIVVDLPPEEADLLEGPAADHGLDIIRLVAPTTDD
TRLPLVCRHASGFIYYVSITGVTGTRTASADELAQALPRLRAATDLPVAIGFGIRTPEQAAAAVRVADAAVVASALIATL
EDSLDAEGRAGPDTLKRVLAQLEALGRAVRNART
>Mature_273_residues
SRIARRFAALKAQDRGALIPYLQACDPDYETSLALLRAMPGAGADLIEIGVPFSDPSADGPTIQAAARRGLAAGATMARV
LEMVTRFREGDADTPIVLMGYLNPIEAYGPARFCADAARAGVDGLIVVDLPPEEADLLEGPAADHGLDIIRLVAPTTDDT
RLPLVCRHASGFIYYVSITGVTGTRTASADELAQALPRLRAATDLPVAIGFGIRTPEQAAAAVRVADAAVVASALIATLE
DSLDAEGRAGPDTLKRVLAQLEALGRAVRNART

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=246, Percent_Identity=35.7723577235772, Blast_Score=145, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321412, Length=245, Percent_Identity=42.8571428571429, Blast_Score=201, Evalue=1e-52,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_GLUDA (A9HE84)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601510.1
- RefSeq:   YP_002276340.1
- ProteinModelPortal:   A9HE84
- SMR:   A9HE84
- GeneID:   5789174
- GeneID:   6975391
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1965
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28638; Mature: 28507

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIARRFAALKAQDRGALIPYLQACDPDYETSLALLRAMPGAGADLIEIGVPFSDPSAD
CHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
GPTIQAAARRGLAAGATMARVLEMVTRFREGDADTPIVLMGYLNPIEAYGPARFCADAAR
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHHHHHH
AGVDGLIVVDLPPEEADLLEGPAADHGLDIIRLVAPTTDDTRLPLVCRHASGFIYYVSIT
CCCCCEEEEECCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCEEEEEEEE
GVTGTRTASADELAQALPRLRAATDLPVAIGFGIRTPEQAAAAVRVADAAVVASALIATL
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EDSLDAEGRAGPDTLKRVLAQLEALGRAVRNART
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRIARRFAALKAQDRGALIPYLQACDPDYETSLALLRAMPGAGADLIEIGVPFSDPSAD
HHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCC
GPTIQAAARRGLAAGATMARVLEMVTRFREGDADTPIVLMGYLNPIEAYGPARFCADAAR
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHHHHHH
AGVDGLIVVDLPPEEADLLEGPAADHGLDIIRLVAPTTDDTRLPLVCRHASGFIYYVSIT
CCCCCEEEEECCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCEEEEEEEE
GVTGTRTASADELAQALPRLRAATDLPVAIGFGIRTPEQAAAAVRVADAAVVASALIATL
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EDSLDAEGRAGPDTLKRVLAQLEALGRAVRNART
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA