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The map label for this gene is manX [C]

Identifier: 162146983

GI number: 162146983

Start: 1184329

End: 1184826

Strand: Reverse

Name: manX [C]

Synonym: GDI_1188

Alternate gene names: 162146983

Gene position: 1184826-1184329 (Counterclockwise)

Preceding gene: 162146984

Following gene: 162146982

Centisome position: 30.04

GC content: 68.47

Gene sequence:

>498_bases
ATGATTGGTATGGTCCTCGTCACCCATGGCGAGCTCGGCGACACGCTGAAACGCGCGATGGAGCACGTGGTGGGGCCGCA
ACCCCAACTGGCTACCCTGAACATCGAGGCTGGTGACGATCCCGTCCTGCGACGCGCGGACCTGCAATCGGTGATCGCCG
GCGTCGATACGGGTGATGGCGTCCTGCTGCTGACCGACATGTTCGGCAGCACGGCCTCGAACCTCGCCATTTCCACGATG
GAAGCGGGCCGGGTGGAAGTCCTGGCCGGCGTCAACGTGCCGATGATGGTCAAGCTGGCGCAGATCCGCAACGGCCACAC
GCTGGAGGAATGCGCGGACCTGGCCGAGGGCGCGGGCCGCAAATACATCTCCACCGCCTCGCACCTGCCGCCGCAATGCC
TGGGGGGCGCCCGGAGCTGCCTGCTGGGCGAACGCCCCGCCGACATGCCGCCGCAGGCGCTGTACCGTCCTGCGCCGCGG
CGGGTCGCCATGGGCTGA

Upstream 100 bases:

>100_bases
TCATGCACCGCGAACTGGCCCGGCAGGGGCGATCTAGCTGGCGCTGGGCCAGCCGACCGAGGGAGCACGCGGCGGACGTG
GTGGAAGGGCAGTATTCCTC

Downstream 100 bases:

>100_bases
CCGGGCATGTCCTCGCCCGGCATGTCGGCTCCTGGCGGGCTGGACCCCCAGGACCCCGATGTCCTGTCCGTGACCCTGAC
CATGGTCAATCGTCGCGGGC

Product: phosphotransferase enzyme IIA

Products: protein histidine; sugar phosphate

Alternate protein names: PTS System IIA Component; PTS System IIA Component Man Family; PTS System Fructose IIA Component; Fructose-Specific IIA Component; Protein-N P-Phosphohistidine-Sugar Phosphotransferase; Pts System Mannose-Specific Eiiab Component; PTS Mannose-Specific IIA Component; PTS System Mannose Subfamily IIA Subunit; IIA Component Of Sugar Transport PTS System; Pts System Fructose Subfamily IIa Component; Pts System Mannose/Fructose/Sorbose Family IIa Component; PTS System Fructose Subfamily Transprotein Subunit IIA; Pts System Fructose IIA Component; PTS System Mannose/Fructose-Specific Component IIA; PTS System Mannose/Fructose/Sorbose Family IIA Subunit; Phosphotransferase System Fructose Subfamily IIA Component; PTS System Transporter Subunit IIA; PTS System Fructose Subfamily Transporter Subunit IIA; PTS System Mannose-Type Transporter Subunit IIA; Pentitol Phosphotransferase IIA Component; PTS System Component IIA; Phosphotransferase System IIA Component; Phosphotransferase Mannose/Fructose-Specific Component IIA; PTS System Mannose/Fructose-Specific IIA Component; PTS System IIa Component; Phosphotransferase System Fructose-Specific IIA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIGMVLVTHGELGDTLKRAMEHVVGPQPQLATLNIEAGDDPVLRRADLQSVIAGVDTGDGVLLLTDMFGSTASNLAISTM
EAGRVEVLAGVNVPMMVKLAQIRNGHTLEECADLAEGAGRKYISTASHLPPQCLGGARSCLLGERPADMPPQALYRPAPR
RVAMG

Sequences:

>Translated_165_residues
MIGMVLVTHGELGDTLKRAMEHVVGPQPQLATLNIEAGDDPVLRRADLQSVIAGVDTGDGVLLLTDMFGSTASNLAISTM
EAGRVEVLAGVNVPMMVKLAQIRNGHTLEECADLAEGAGRKYISTASHLPPQCLGGARSCLLGERPADMPPQALYRPAPR
RVAMG
>Mature_165_residues
MIGMVLVTHGELGDTLKRAMEHVVGPQPQLATLNIEAGDDPVLRRADLQSVIAGVDTGDGVLLLTDMFGSTASNLAISTM
EAGRVEVLAGVNVPMMVKLAQIRNGHTLEECADLAEGAGRKYISTASHLPPQCLGGARSCLLGERPADMPPQALYRPAPR
RVAMG

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 17351; Mature: 17351

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGMVLVTHGELGDTLKRAMEHVVGPQPQLATLNIEAGDDPVLRRADLQSVIAGVDTGDG
CCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
VLLLTDMFGSTASNLAISTMEAGRVEVLAGVNVPMMVKLAQIRNGHTLEECADLAEGAGR
EEEEEECCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
KYISTASHLPPQCLGGARSCLLGERPADMPPQALYRPAPRRVAMG
CCHHHHHCCCHHHHCHHHHHHCCCCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIGMVLVTHGELGDTLKRAMEHVVGPQPQLATLNIEAGDDPVLRRADLQSVIAGVDTGDG
CCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
VLLLTDMFGSTASNLAISTMEAGRVEVLAGVNVPMMVKLAQIRNGHTLEECADLAEGAGR
EEEEEECCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
KYISTASHLPPQCLGGARSCLLGERPADMPPQALYRPAPRRVAMG
CCHHHHHCCCHHHHCHHHHHHCCCCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA