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The map label for this gene is ilvE [H]

Identifier: 162146979

GI number: 162146979

Start: 1179950

End: 1180753

Strand: Reverse

Name: ilvE [H]

Synonym: GDI_1184

Alternate gene names: 162146979

Gene position: 1180753-1179950 (Counterclockwise)

Preceding gene: 162146980

Following gene: 162146978

Centisome position: 29.94

GC content: 71.52

Gene sequence:

>804_bases
ATGCTGTGGCTGAATGACGGGCTGGTCCCGGCGGACCGGGCGCGGATCGATCCCGCCGATCGCGGCCTGACCCTGGGTGA
CGGACTGTTCGAGACGATGCGCGTCGCGGGGGGGCGCATCGCCCATCTGGCCCGGCATCTCGACCGGCTGTGCCAGGGTG
CGGACCTGCTGATGTTTCCGGGCCCCGACCTGGACCGGATCGAGGACGCGTTGCGCCAGGTGGTGCGGCACAATGCGCTG
GCCGAAGGCGCGCTGCGCCTGACTCTGACGCGCGGCTGCGGCCCGCGCGGCCTGTTCCCGCCCGCCTCTCCGCAACCCAG
CCTGCTGATCGTGCCCTTTCCCCCGGCGCCCGCCCCGGGACCGGCCCGCCTGGTGGTCAGCACCCGCGTCCGGCGGGATG
CGGCATCCCCGCTCTCGCGGGTCAAGAGCCTGAATTACCTGCCCAACATCCTGGCCCGGCTGGAGGCCGAACAGCGCGGC
GCGGATGACGCGCTGCTGCTGAACGCGGCGGGGCGGGTGGCCGAAAGCACGGTCAGCACGGTGCTGGTCCATCACCAGGG
GCGCCTGGTCACGCCGCCGGTCGGCGACGGCGCCTTGCCGGGGATCGCTCGCGCCGTGCTGCTGGCGGCTTGCATGGTGG
AGGAGGCCTCGCTGATACCCGACGATCTGCGGGGGGCAGGGGGTGTGGTGCTGGTCAACAGCCTGTCGGTGCGTCCGGTT
GTCAGTCTGGACGGGATGGCGTTGTCTGTTCTGCCGGGGTTGGTCGAGGACATTCGGGCGTGTGTACAAGGGCTCAGCCC
TTGA

Upstream 100 bases:

>100_bases
TCCTGTCGGACCCCGGGCGGGAATATGCGGAAATGTGCCTGAAGATCGCGCCGCTGCTGGCCCTGTTCGGGGCCGAGCCG
GCCGGGATGGCGTCATGACG

Downstream 100 bases:

>100_bases
CCCGCCAAAGGGCCGAGCCCTTTGGAAACCCAATCGTTCGGGGTGCGGGGCATCATGCCCAGCCTTGCTCCCGTGGTCCA
GGTTGGATAAGGACGCGGGC

Product: aminotransferase, class IV

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MLWLNDGLVPADRARIDPADRGLTLGDGLFETMRVAGGRIAHLARHLDRLCQGADLLMFPGPDLDRIEDALRQVVRHNAL
AEGALRLTLTRGCGPRGLFPPASPQPSLLIVPFPPAPAPGPARLVVSTRVRRDAASPLSRVKSLNYLPNILARLEAEQRG
ADDALLLNAAGRVAESTVSTVLVHHQGRLVTPPVGDGALPGIARAVLLAACMVEEASLIPDDLRGAGGVVLVNSLSVRPV
VSLDGMALSVLPGLVEDIRACVQGLSP

Sequences:

>Translated_267_residues
MLWLNDGLVPADRARIDPADRGLTLGDGLFETMRVAGGRIAHLARHLDRLCQGADLLMFPGPDLDRIEDALRQVVRHNAL
AEGALRLTLTRGCGPRGLFPPASPQPSLLIVPFPPAPAPGPARLVVSTRVRRDAASPLSRVKSLNYLPNILARLEAEQRG
ADDALLLNAAGRVAESTVSTVLVHHQGRLVTPPVGDGALPGIARAVLLAACMVEEASLIPDDLRGAGGVVLVNSLSVRPV
VSLDGMALSVLPGLVEDIRACVQGLSP
>Mature_267_residues
MLWLNDGLVPADRARIDPADRGLTLGDGLFETMRVAGGRIAHLARHLDRLCQGADLLMFPGPDLDRIEDALRQVVRHNAL
AEGALRLTLTRGCGPRGLFPPASPQPSLLIVPFPPAPAPGPARLVVSTRVRRDAASPLSRVKSLNYLPNILARLEAEQRG
ADDALLLNAAGRVAESTVSTVLVHHQGRLVTPPVGDGALPGIARAVLLAACMVEEASLIPDDLRGAGGVVLVNSLSVRPV
VSLDGMALSVLPGLVEDIRACVQGLSP

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=266, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787338, Length=250, Percent_Identity=26.4, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 28066; Mature: 28066

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWLNDGLVPADRARIDPADRGLTLGDGLFETMRVAGGRIAHLARHLDRLCQGADLLMFP
CEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEC
GPDLDRIEDALRQVVRHNALAEGALRLTLTRGCGPRGLFPPASPQPSLLIVPFPPAPAPG
CCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCC
PARLVVSTRVRRDAASPLSRVKSLNYLPNILARLEAEQRGADDALLLNAAGRVAESTVST
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
VLVHHQGRLVTPPVGDGALPGIARAVLLAACMVEEASLIPDDLRGAGGVVLVNSLSVRPV
HHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCEE
VSLDGMALSVLPGLVEDIRACVQGLSP
EEECCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLWLNDGLVPADRARIDPADRGLTLGDGLFETMRVAGGRIAHLARHLDRLCQGADLLMFP
CEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEC
GPDLDRIEDALRQVVRHNALAEGALRLTLTRGCGPRGLFPPASPQPSLLIVPFPPAPAPG
CCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCC
PARLVVSTRVRRDAASPLSRVKSLNYLPNILARLEAEQRGADDALLLNAAGRVAESTVST
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHH
VLVHHQGRLVTPPVGDGALPGIARAVLLAACMVEEASLIPDDLRGAGGVVLVNSLSVRPV
HHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCEE
VSLDGMALSVLPGLVEDIRACVQGLSP
EEECCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]