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The map label for this gene is purH [H]

Identifier: 162146978

GI number: 162146978

Start: 1178236

End: 1179813

Strand: Reverse

Name: purH [H]

Synonym: GDI_1183

Alternate gene names: 162146978

Gene position: 1179813-1178236 (Counterclockwise)

Preceding gene: 162146979

Following gene: 162146977

Centisome position: 29.91

GC content: 71.55

Gene sequence:

>1578_bases
ATGACCCAGACGCCCGTGCCGGTCCGCCGCGCCCTGATTTCCGTTTCCGACAAGGCGGGGTTGCTCGACCTCGCCCGCGC
CCTGATCGCCCATGGGGCGGAAATCCTCTCGACCGGGGGCTCGGCCCGTGCGCTGCGCGAGGCCGGACTGAAGGTGACCG
AGGTGTCGGACCATACCGGCTTTCCCGAAATTCTCGACGGGCGGGTGAAGACCCTGGTGCCGCAGATCCATGGCGGCATC
CTGGGCCGCCGCGACCTGCCGGCCCATCTGGCGCAGATGGACGAACACGGGATCGCGCCGATCGACCTGGTCGCGGTGAA
CCTCTACCCGTTCGAGGCCACGGTGGCCTCCGGCGCGGGGGAAGAGGACTGCATCGAGAACATCGATATCGGCGGCCCCG
CCCTGATCCGCGCGGCGGCCAAGAACCACGGCCATGTCGTGGTGCTGACCGATCCGGCGCAGTACGGCGCGGTGATCGAC
GCGCTGGCCCAGGGCGGCACCACGCTTGCGGCGCGGCGCGCGCTGGCCGGCGCGGCCTATGCCCGCACCGCCGCCTATGA
TTCCGCCATCGCGGCGTGGTTCGCCGTGCAGCGCGGCGACGTGCTGCCGGAACGCCTGGCCGTCGCGGGCCTGCGCCGCG
AAAGCCTGCGCTATGGCGAAAATCCGCACCAGCAGGCGGCCTTCTATGCCGACGGCAGCAGCCGGCCCGGTGTGGCCACC
GCCCGCCAGGTGCAGGGCAAATCCCTGTCCTACAACAACCTGAACGATACCGATGCGGCGTTCGAGGCCGTGGCGGAATT
CGACGGCCCGGCGGTGGTGATCGTCAAGCACGCCAATCCCTGCGGCGTCGCCACCGCCGATACGCTGTCGGCGGCCTGGG
ACCTGGCGCTGCGCTGCGATCCGGTCTCGGCCTTCGGCGGCATCGTCGCGCTGAACCGCACGCTGGACGCCGACGCCGCC
GCGCGCATCGCGGCCATCTTCACCGAGGTCATCGTCGCCCCCGACGCGACGGAGGAGGCCCAGGCGATCCTGGCGAAGAA
GAAGAACCTGCGCCTGCTGCTGACCGGCGCGATGCCCGACCCGTCCGTGGGCGGGGTGGCCATCCGTTCGGTCGCCGGCG
GCTTCCTGGCGCAGACCCGCGACAATGGCCGGATCGTCCCCGCCGGCCTGAAGGTGGTGACCCGCCGCGCCCCGACCGAG
GCCGAGATGGCGGATCTGATCTTCGCCTTCCGCGTCGGCAAGCATGTGAAGTCGAACGCCATCGTCTATGCCAAGGGCCA
GGCGACCGCCGGCATCGGCGCGGGGCAGATGAGCCGCGTGGACTCGGCGCGCATCGCCGCGATCAAGGGGGCGGAAGCCG
CCCGGGCCGCCGGCCTGGACCAGCCGCTGACGACGGGCAGCGTGGTGGCGTCGGACGCGTTTTTCCCCTTCGCCGACGGG
CTGGAGGCCGCGATCGCGGCCGGCGCCACGGCGGTGATCCAGCCGGGCGGATCGATCCGCGATGACGAGGTCATCGCCGC
CGCCGACCGGGCGGGCATCGCCATGGTGTTCACAGGTATGCGCCACTTCCGGCACTGA

Upstream 100 bases:

>100_bases
TTCGGGGTGCGGGGCATCATGCCCAGCCTTGCTCCCGTGGTCCAGGTTGGATAAGGACGCGGGCTTTGCCGCCCGTCCAT
GTGAAAGGTCGCTTCCCGCG

Downstream 100 bases:

>100_bases
TCCGACGCAAGCGGGGATTGTCGCGCCGGGCGGGATCGACTTGACTGTTCCGCCCCACGCACGACGACCACGCAGAGACG
AGACCAGGCATGGAAATGTT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTQTPVPVRRALISVSDKAGLLDLARALIAHGAEILSTGGSARALREAGLKVTEVSDHTGFPEILDGRVKTLVPQIHGGI
LGRRDLPAHLAQMDEHGIAPIDLVAVNLYPFEATVASGAGEEDCIENIDIGGPALIRAAAKNHGHVVVLTDPAQYGAVID
ALAQGGTTLAARRALAGAAYARTAAYDSAIAAWFAVQRGDVLPERLAVAGLRRESLRYGENPHQQAAFYADGSSRPGVAT
ARQVQGKSLSYNNLNDTDAAFEAVAEFDGPAVVIVKHANPCGVATADTLSAAWDLALRCDPVSAFGGIVALNRTLDADAA
ARIAAIFTEVIVAPDATEEAQAILAKKKNLRLLLTGAMPDPSVGGVAIRSVAGGFLAQTRDNGRIVPAGLKVVTRRAPTE
AEMADLIFAFRVGKHVKSNAIVYAKGQATAGIGAGQMSRVDSARIAAIKGAEAARAAGLDQPLTTGSVVASDAFFPFADG
LEAAIAAGATAVIQPGGSIRDDEVIAAADRAGIAMVFTGMRHFRH

Sequences:

>Translated_525_residues
MTQTPVPVRRALISVSDKAGLLDLARALIAHGAEILSTGGSARALREAGLKVTEVSDHTGFPEILDGRVKTLVPQIHGGI
LGRRDLPAHLAQMDEHGIAPIDLVAVNLYPFEATVASGAGEEDCIENIDIGGPALIRAAAKNHGHVVVLTDPAQYGAVID
ALAQGGTTLAARRALAGAAYARTAAYDSAIAAWFAVQRGDVLPERLAVAGLRRESLRYGENPHQQAAFYADGSSRPGVAT
ARQVQGKSLSYNNLNDTDAAFEAVAEFDGPAVVIVKHANPCGVATADTLSAAWDLALRCDPVSAFGGIVALNRTLDADAA
ARIAAIFTEVIVAPDATEEAQAILAKKKNLRLLLTGAMPDPSVGGVAIRSVAGGFLAQTRDNGRIVPAGLKVVTRRAPTE
AEMADLIFAFRVGKHVKSNAIVYAKGQATAGIGAGQMSRVDSARIAAIKGAEAARAAGLDQPLTTGSVVASDAFFPFADG
LEAAIAAGATAVIQPGGSIRDDEVIAAADRAGIAMVFTGMRHFRH
>Mature_524_residues
TQTPVPVRRALISVSDKAGLLDLARALIAHGAEILSTGGSARALREAGLKVTEVSDHTGFPEILDGRVKTLVPQIHGGIL
GRRDLPAHLAQMDEHGIAPIDLVAVNLYPFEATVASGAGEEDCIENIDIGGPALIRAAAKNHGHVVVLTDPAQYGAVIDA
LAQGGTTLAARRALAGAAYARTAAYDSAIAAWFAVQRGDVLPERLAVAGLRRESLRYGENPHQQAAFYADGSSRPGVATA
RQVQGKSLSYNNLNDTDAAFEAVAEFDGPAVVIVKHANPCGVATADTLSAAWDLALRCDPVSAFGGIVALNRTLDADAAA
RIAAIFTEVIVAPDATEEAQAILAKKKNLRLLLTGAMPDPSVGGVAIRSVAGGFLAQTRDNGRIVPAGLKVVTRRAPTEA
EMADLIFAFRVGKHVKSNAIVYAKGQATAGIGAGQMSRVDSARIAAIKGAEAARAAGLDQPLTTGSVVASDAFFPFADGL
EAAIAAGATAVIQPGGSIRDDEVIAAADRAGIAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=611, Percent_Identity=35.0245499181669, Blast_Score=274, Evalue=1e-73,
Organism=Escherichia coli, GI1790439, Length=538, Percent_Identity=56.1338289962825, Blast_Score=547, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71985564, Length=601, Percent_Identity=34.2762063227953, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI71985556, Length=317, Percent_Identity=27.1293375394322, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71985574, Length=303, Percent_Identity=28.3828382838284, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323768, Length=602, Percent_Identity=32.890365448505, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6323056, Length=603, Percent_Identity=33.0016583747927, Blast_Score=260, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24649832, Length=600, Percent_Identity=36, Blast_Score=281, Evalue=8e-76,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54217; Mature: 54086

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTPVPVRRALISVSDKAGLLDLARALIAHGAEILSTGGSARALREAGLKVTEVSDHTG
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHCCCEEEEECCCCC
FPEILDGRVKTLVPQIHGGILGRRDLPAHLAQMDEHGIAPIDLVAVNLYPFEATVASGAG
CHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECHHHHCCCCC
EEDCIENIDIGGPALIRAAAKNHGHVVVLTDPAQYGAVIDALAQGGTTLAARRALAGAAY
CHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ARTAAYDSAIAAWFAVQRGDVLPERLAVAGLRRESLRYGENPHQQAAFYADGSSRPGVAT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCH
ARQVQGKSLSYNNLNDTDAAFEAVAEFDGPAVVIVKHANPCGVATADTLSAAWDLALRCD
HHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHEEEEEC
PVSAFGGIVALNRTLDADAAARIAAIFTEVIVAPDATEEAQAILAKKKNLRLLLTGAMPD
CHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCC
PSVGGVAIRSVAGGFLAQTRDNGRIVPAGLKVVTRRAPTEAEMADLIFAFRVGKHVKSNA
CCCCCHHHHHHHCCHHEEECCCCEEEECCHHEEECCCCCHHHHHHHHHHHHHCCCCCCCE
IVYAKGQATAGIGAGQMSRVDSARIAAIKGAEAARAAGLDQPLTTGSVVASDAFFPFADG
EEEECCCCCCCCCCCHHHHCCCHHEEEECCCHHHHHCCCCCCCCCCCEEECCCCCCCCCC
LEAAIAAGATAVIQPGGSIRDDEVIAAADRAGIAMVFTGMRHFRH
HHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TQTPVPVRRALISVSDKAGLLDLARALIAHGAEILSTGGSARALREAGLKVTEVSDHTG
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHCCCEEEEECCCCC
FPEILDGRVKTLVPQIHGGILGRRDLPAHLAQMDEHGIAPIDLVAVNLYPFEATVASGAG
CHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEECHHHHCCCCC
EEDCIENIDIGGPALIRAAAKNHGHVVVLTDPAQYGAVIDALAQGGTTLAARRALAGAAY
CHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ARTAAYDSAIAAWFAVQRGDVLPERLAVAGLRRESLRYGENPHQQAAFYADGSSRPGVAT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCH
ARQVQGKSLSYNNLNDTDAAFEAVAEFDGPAVVIVKHANPCGVATADTLSAAWDLALRCD
HHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHEEEEEC
PVSAFGGIVALNRTLDADAAARIAAIFTEVIVAPDATEEAQAILAKKKNLRLLLTGAMPD
CHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCC
PSVGGVAIRSVAGGFLAQTRDNGRIVPAGLKVVTRRAPTEAEMADLIFAFRVGKHVKSNA
CCCCCHHHHHHHCCHHEEECCCCEEEECCHHEEECCCCCHHHHHHHHHHHHHCCCCCCCE
IVYAKGQATAGIGAGQMSRVDSARIAAIKGAEAARAAGLDQPLTTGSVVASDAFFPFADG
EEEECCCCCCCCCCCHHHHCCCHHEEEECCCHHHHHCCCCCCCCCCCEEECCCCCCCCCC
LEAAIAAGATAVIQPGGSIRDDEVIAAADRAGIAMVFTGMRHFRH
HHHHHHCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA