Click here to switch to the map view.

The map label for this gene is benM [H]

Identifier: 162146906

GI number: 162146906

Start: 1098466

End: 1099344

Strand: Reverse

Name: benM [H]

Synonym: GDI_1105

Alternate gene names: 162146906

Gene position: 1099344-1098466 (Counterclockwise)

Preceding gene: 162146907

Following gene: 162146902

Centisome position: 27.87

GC content: 53.7

Gene sequence:

>879_bases
ATGCGCAGCGCAAATCTTGAATATTTCAAACTGGCTGTGGAGGCCGGCAGCCTGACGCGGGCAGCCGGCCTCCATGGTGT
GAGAGTTTCCACGTTCACGCGTGCAATCGACAAGCTCGAAGACGAGTTTGGTGTTACCTTACTGGAGCGAAACCATGCCG
GGGTCCGCCTTACGGCAGCCGGTGACTTATTGTTTCGGAGAATCGTCACGCTGCTTGATGATCTGGATGAAGTCAAAGCC
TTATGCCAAATGCTTGGAACAGGTCGCCGCGGGGTCGTTCGGATCGGTACCCTACTCCCTCCGGTCGGCGTCCAATTCAG
GCTTGCATTATCGGTTTGGAAGTCGAAACATCCAGACATAAGAGTGATTTTTTACGAGATGGGAACACGAGACCTTCGAT
CCGCCTTGTTTCGTCGTCGTGTAGATGTGATTTTTTCCACGCCTTTTGCCAAATCCGGAGCGATTGAATCACTTCCGTTT
TGCAGCGAGAACTTGATGGTGGCGCTGTCGAGCGAACACTCTCTGCATCAATATCCGAGTATCACGCGGGGACAATTGCG
AAATGAGACTTTCTTGGTCCAGGACTGGGGGAGCGATCATAGTGTAAGGAACCATTATATGGAGATTCTCGGCAAGGATG
TGAAGATTGAAACGCATCCTGCGGGAAAACAATCCGTCTTCGCGCTGGTAAGTGCCGGATATGGCGTGACACTTGCCGTC
CAGAGTCAAGCGGAACGAGGCTTTCCGGGCGTCATTTTCCGGCCGCTACGGGAAAGTGATGCCCACCTGCCCATTCGCCT
CGGCTGGGATGCCGGTCGCCAAGACGCGGTTACGGGCCGTTTCGTTGCTTTTATCCGCGATTTTGTTCGACAGCGCTAA

Upstream 100 bases:

>100_bases
ATCAACCGAGCATGGCAGAGGGTTGGCTCGTTTCTCTTCTGCATACTTATTACTCTGCTTGTTCGTGGGGCAGAGATATT
CGCACGTCCCCGTTCACGTC

Downstream 100 bases:

>100_bases
TTCGAAGACGCCGCGCCTTAGAAAACGCTCTGTCACCCTCCATGAAACGCGCGAGCATCGGCGGGATCAGCTTCGCCGGT
GCGATTTCAGCCTGACCGTT

Product: HTH-type transcriptional regulator benM (Ben and cat opero transcriptional regulator)

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MRSANLEYFKLAVEAGSLTRAAGLHGVRVSTFTRAIDKLEDEFGVTLLERNHAGVRLTAAGDLLFRRIVTLLDDLDEVKA
LCQMLGTGRRGVVRIGTLLPPVGVQFRLALSVWKSKHPDIRVIFYEMGTRDLRSALFRRRVDVIFSTPFAKSGAIESLPF
CSENLMVALSSEHSLHQYPSITRGQLRNETFLVQDWGSDHSVRNHYMEILGKDVKIETHPAGKQSVFALVSAGYGVTLAV
QSQAERGFPGVIFRPLRESDAHLPIRLGWDAGRQDAVTGRFVAFIRDFVRQR

Sequences:

>Translated_292_residues
MRSANLEYFKLAVEAGSLTRAAGLHGVRVSTFTRAIDKLEDEFGVTLLERNHAGVRLTAAGDLLFRRIVTLLDDLDEVKA
LCQMLGTGRRGVVRIGTLLPPVGVQFRLALSVWKSKHPDIRVIFYEMGTRDLRSALFRRRVDVIFSTPFAKSGAIESLPF
CSENLMVALSSEHSLHQYPSITRGQLRNETFLVQDWGSDHSVRNHYMEILGKDVKIETHPAGKQSVFALVSAGYGVTLAV
QSQAERGFPGVIFRPLRESDAHLPIRLGWDAGRQDAVTGRFVAFIRDFVRQR
>Mature_292_residues
MRSANLEYFKLAVEAGSLTRAAGLHGVRVSTFTRAIDKLEDEFGVTLLERNHAGVRLTAAGDLLFRRIVTLLDDLDEVKA
LCQMLGTGRRGVVRIGTLLPPVGVQFRLALSVWKSKHPDIRVIFYEMGTRDLRSALFRRRVDVIFSTPFAKSGAIESLPF
CSENLMVALSSEHSLHQYPSITRGQLRNETFLVQDWGSDHSVRNHYMEILGKDVKIETHPAGKQSVFALVSAGYGVTLAV
QSQAERGFPGVIFRPLRESDAHLPIRLGWDAGRQDAVTGRFVAFIRDFVRQR

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI2367136, Length=261, Percent_Identity=27.2030651340996, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787879, Length=283, Percent_Identity=26.8551236749117, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790399, Length=278, Percent_Identity=26.978417266187, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=234, Percent_Identity=23.9316239316239, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788887, Length=297, Percent_Identity=24.9158249158249, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=174, Percent_Identity=25.8620689655172, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787806, Length=296, Percent_Identity=25.3378378378378, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32647; Mature: 32647

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSANLEYFKLAVEAGSLTRAAGLHGVRVSTFTRAIDKLEDEFGVTLLERNHAGVRLTAA
CCCCCCEEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEH
GDLLFRRIVTLLDDLDEVKALCQMLGTGRRGVVRIGTLLPPVGVQFRLALSVWKSKHPDI
HHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCE
RVIFYEMGTRDLRSALFRRRVDVIFSTPFAKSGAIESLPFCSENLMVALSSEHSLHQYPS
EEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCC
ITRGQLRNETFLVQDWGSDHSVRNHYMEILGKDVKIETHPAGKQSVFALVSAGYGVTLAV
CCHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCEEEEE
QSQAERGFPGVIFRPLRESDAHLPIRLGWDAGRQDAVTGRFVAFIRDFVRQR
ECCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSANLEYFKLAVEAGSLTRAAGLHGVRVSTFTRAIDKLEDEFGVTLLERNHAGVRLTAA
CCCCCCEEEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEH
GDLLFRRIVTLLDDLDEVKALCQMLGTGRRGVVRIGTLLPPVGVQFRLALSVWKSKHPDI
HHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCE
RVIFYEMGTRDLRSALFRRRVDVIFSTPFAKSGAIESLPFCSENLMVALSSEHSLHQYPS
EEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCC
ITRGQLRNETFLVQDWGSDHSVRNHYMEILGKDVKIETHPAGKQSVFALVSAGYGVTLAV
CCHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCEEEEE
QSQAERGFPGVIFRPLRESDAHLPIRLGWDAGRQDAVTGRFVAFIRDFVRQR
ECCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]