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The map label for this gene is apbA [H]

Identifier: 162146827

GI number: 162146827

Start: 1024290

End: 1025183

Strand: Direct

Name: apbA [H]

Synonym: GDI_1009

Alternate gene names: 162146827

Gene position: 1024290-1025183 (Clockwise)

Preceding gene: 162146825

Following gene: 162146838

Centisome position: 25.97

GC content: 60.29

Gene sequence:

>894_bases
ATGGCAACGGCGGGACATGATGTCACGTTCCTGGTGCGTCCCAACCGGCTGCAGCAGCTGCGTACGGACGGGCTACGGCT
GGTCAGTCCGCTGGGGGACGCCAGCCTCGCGCCGAAAATGGTGACATCCGACGATATCGATGGCCCTTATGACATCATTC
TTCTCAGCGTAAAGGCTTACTCCCTCGCGTCCGCGATGGACGATTTCGCTGCCGCCGTCGGCCCGGATACGCGCATCATA
CCTCTTCTAAATGGCATGCGGCATCTCGATGTACTGGCAGAGCGTTTCGGAGATCCGAAGGTCCTGGGCGGGACGTGCTT
CATCGTCAGCAAGCTGGATTCTGAAGGACATGTCGTTCAAGTGGGATCACTCCCCAAACTGACCTTTGGCCCGCGATCCT
CTGGCGACAACGCCCCGATCCGGAGCCTCAAGGATGCTCTCTCCGGTCCTGGCTTCGAGACTATATTGTCGGACCACATC
ATTCAGGAGATGTGGAACAAATGGGCTCTGCTGGCATCGCTGGGCACCGTATGTTGCCTGATGCGGGCCACCGTGGGGGA
AGTGGCCAGCGCGCCCGCAGGAGAGGCTTTCGCGCGGGCGACGATCGGCGAATGCGCTTCGATCGCGGCGGCGGCCGGGT
ATCCGCTCACCGAGGAGACCCTGGGCGGTATCGTCCGGCTGTTAATCGCAAAGGAATCGTCCCTGACATCGTCCATGTTC
CGTGATCTGTCACAGGGCGCTCCCATTGAGGCGACACAGATCATTGGCGACCTCGTGAAGCGGGCCCGCGCGCATCAGAT
TGCCACGCCGACTCTTGATCTGATCAATCTCAATCTCACTGTCTACGAGCAGAGAAGGCTGCGCGTATCTTCGGTGGCCG
AGCAGAGTATCTGA

Upstream 100 bases:

>100_bases
GGCCTCGCGGCGCGGCAGTCCCGGAACAAGGAACGACACCATGAATCCAAAAGTTCTGATCGTCGGCGCAGGTGCAGTTG
GGGGATATTTTGGGGCCCGG

Downstream 100 bases:

>100_bases
TCGGATTCTCGCAATATCCGGCAATGGAACGATCGAAAGTCACTTTCCATATTCGTCGACGCCTAAATAACCGGAAACCT
CTCGAAGATATTGAGGCGCA

Product: 2-dehydropantoate 2-reductase

Products: Nicotinamide adenine dinucleotide phosphate; (R)-Pantoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MATAGHDVTFLVRPNRLQQLRTDGLRLVSPLGDASLAPKMVTSDDIDGPYDIILLSVKAYSLASAMDDFAAAVGPDTRII
PLLNGMRHLDVLAERFGDPKVLGGTCFIVSKLDSEGHVVQVGSLPKLTFGPRSSGDNAPIRSLKDALSGPGFETILSDHI
IQEMWNKWALLASLGTVCCLMRATVGEVASAPAGEAFARATIGECASIAAAAGYPLTEETLGGIVRLLIAKESSLTSSMF
RDLSQGAPIEATQIIGDLVKRARAHQIATPTLDLINLNLTVYEQRRLRVSSVAEQSI

Sequences:

>Translated_297_residues
MATAGHDVTFLVRPNRLQQLRTDGLRLVSPLGDASLAPKMVTSDDIDGPYDIILLSVKAYSLASAMDDFAAAVGPDTRII
PLLNGMRHLDVLAERFGDPKVLGGTCFIVSKLDSEGHVVQVGSLPKLTFGPRSSGDNAPIRSLKDALSGPGFETILSDHI
IQEMWNKWALLASLGTVCCLMRATVGEVASAPAGEAFARATIGECASIAAAAGYPLTEETLGGIVRLLIAKESSLTSSMF
RDLSQGAPIEATQIIGDLVKRARAHQIATPTLDLINLNLTVYEQRRLRVSSVAEQSI
>Mature_296_residues
ATAGHDVTFLVRPNRLQQLRTDGLRLVSPLGDASLAPKMVTSDDIDGPYDIILLSVKAYSLASAMDDFAAAVGPDTRIIP
LLNGMRHLDVLAERFGDPKVLGGTCFIVSKLDSEGHVVQVGSLPKLTFGPRSSGDNAPIRSLKDALSGPGFETILSDHII
QEMWNKWALLASLGTVCCLMRATVGEVASAPAGEAFARATIGECASIAAAAGYPLTEETLGGIVRLLIAKESSLTSSMFR
DLSQGAPIEATQIIGDLVKRARAHQIATPTLDLINLNLTVYEQRRLRVSSVAEQSI

Specific function: Catalyzes The NADPH-Dependent Reduction Of Ketopantoate Into Pantoic Acid. [C]

COG id: COG1893

COG function: function code H; Ketopantoate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ketopantoate reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR003710
- InterPro:   IPR013752
- InterPro:   IPR013332
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02558 ApbA; PF08546 ApbA_C [H]

EC number: 1.1.1.169 [C]

Molecular weight: Translated: 31578; Mature: 31447

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAGHDVTFLVRPNRLQQLRTDGLRLVSPLGDASLAPKMVTSDDIDGPYDIILLSVKAY
CCCCCCCEEEEECCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHH
SLASAMDDFAAAVGPDTRIIPLLNGMRHLDVLAERFGDPKVLGGTCFIVSKLDSEGHVVQ
HHHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHHHCCCCCEECCEEEEEEEECCCCCEEE
VGSLPKLTFGPRSSGDNAPIRSLKDALSGPGFETILSDHIIQEMWNKWALLASLGTVCCL
ECCCCCEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRATVGEVASAPAGEAFARATIGECASIAAAAGYPLTEETLGGIVRLLIAKESSLTSSMF
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
RDLSQGAPIEATQIIGDLVKRARAHQIATPTLDLINLNLTVYEQRRLRVSSVAEQSI
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATAGHDVTFLVRPNRLQQLRTDGLRLVSPLGDASLAPKMVTSDDIDGPYDIILLSVKAY
CCCCCCEEEEECCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHH
SLASAMDDFAAAVGPDTRIIPLLNGMRHLDVLAERFGDPKVLGGTCFIVSKLDSEGHVVQ
HHHHHHHHHHHHCCCCCEEHHHHCCHHHHHHHHHHCCCCCEECCEEEEEEEECCCCCEEE
VGSLPKLTFGPRSSGDNAPIRSLKDALSGPGFETILSDHIIQEMWNKWALLASLGTVCCL
ECCCCCEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRATVGEVASAPAGEAFARATIGECASIAAAAGYPLTEETLGGIVRLLIAKESSLTSSMF
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHH
RDLSQGAPIEATQIIGDLVKRARAHQIATPTLDLINLNLTVYEQRRLRVSSVAEQSI
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.0 {NADPH}} [C]

Substrates: 2-Dehydropantoate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 2-Dehydropantoate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> Nicotinamide adenine dinucleotide phosphate + (R)-Pantoate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]