Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 162146741

GI number: 162146741

Start: 931099

End: 933327

Strand: Direct

Name: nadE [H]

Synonym: GDI_0919

Alternate gene names: 162146741

Gene position: 931099-933327 (Clockwise)

Preceding gene: 162146739

Following gene: 162146743

Centisome position: 23.61

GC content: 68.95

Gene sequence:

>2229_bases
ATGGAGATATCCTCCTCCGGGTCATGTGTATGGACCGTCCGGTCCATGGGGGTTTCAATGCGTGGCGCACCCTATGGTTG
CGTCGGCGGGTTGCCGGGGCAGGGCGCCGGGTCGCCTGCCATGGATGTCAAACGAGCGTGGTCGGGTGATCCGCTCCAGC
CGGGTGATCGTATCGTGGCGGATTTTCGCTCCCTCTATCGTCATGGTTTCGCGCGGGTCGCCGGTTGCACCCTGCCGGTG
GCACTGGCCGATCCGGCGACCAACGTCGCGCGGATGGCCGACATGGTCCGGGCCTGCCATGCGGACGGCGTGGCGCTGGC
GGTGTTTCCCGAACTGGGCGTGTCGGGCTACACGATCGAGGACCTGCGCCAGCAGGACGTGCTGCTGGACGGGGTAGGCG
CCGCCCTGGCCGCCCTGGCCGCCGCGACGGCGGACCTTGTGCCGGTTGTCGTGGCGGGCGCGCCGCTGCGCCATGGCGAC
GCGCTGTACAACTGCGCCGTCATCCTGCATCGCGGCACGGTGCTGGGCGTGGTGCCCAAGAGCTACCTGCCCAACTATCG
CGAATTCTACGAGGCCCGGCAGTTCGCGCCCGGCGCCGGCCTGCGCGGCCAGACGATCCATGTCGCGGGCCAGACCGCGC
CCTTCGGCACCGACCTGCTGTTCGAGGCCGAGGACGTGCCCGGCCTGGTGATCGCGATCGAGATCTGCGAGGATCTGTGG
GTTCCCGCGCCGCCCAGCACCGATGCGGCGCTGGCGGGGGCCACGGTGATCGCCAACCCGTCGGCCAGCGACATCACGGT
GGGCAAGGCGGATACGCGGGACCTGCTCTGTCGCTCGCAATCCGCGCGCGCGCTCTGCGCCTATGTCTACGCCGCGGCGG
GCGAGGGGGAATCGACCACCGACCTGGCATGGGACGGCCAGGTCTCGGTCTACGAGAACGGCGCGCTGCTGGCCGAGACG
GTGCGTTTTCCCCAGGGTCCGAACCGGGCGACAGCCGATATCGACCTGGACCTGCTGCGGCAGGAGCGCGCCCGCATGGG
CAGCTTCGCCGACAACCGCGCCGCCCGGGGCCTTCACGCGACAGGGGGCGATACGGGATGGCGGCGCGTCGGCTTCGCCC
TGGCGCCCCCGCCGGGGGATCTGGGGTTGATGCGGCGGATCGAACGATTCCCCTTCGTCCCCGCCGACCCCGCGCGGCTG
GAGCAGGATTGCTATGAAGCGTGGACCATCCAGGTTTCGGCGCTGAAGCAGCGACTGCAGGCCACGGGGACGCGCCGGAT
GGTGATCGGCGTCTCCGGCGGGCTGGATTCCACCCAGGCGCTGCTGGTGGCCGTCCGCGCGGCGGACGAACTGGGCCTGG
GTCGTGACGCGGTGCTGGCCTATACCATGCCGGGATTCGGCACCAGCGCGGGCACGCAGTCCAATGCCATGGCGCTGATG
CAGGCGCTGGGCGTGACGGCGGCGGAACTGGATATCCGGCCGACCGCGCGGATGATGCTGGAACAGATGGGTCATCCCTT
CGCCAGCGGCGTGCCGCAATACGACGTCACGTTCGAGAACGTGCAGGCGGGATTGCGGACCGACTTCCTGTTCCGCCTGG
CCAACCAGCATGGCGGCATCGTGATCGGCACCGGCGACCTGTCGGAACTGGCGCTGGGCTGGTGTACCTATGGCGTGGGC
GACCAGATGTCGCATTACAACGTCAATGCCGGGCTGCCGAAGACGCTGATCCAGCATCTGATCCGCTGGGTCATTTCGGC
GCGGCGGGTGGATGATGACGCGGCCGGCGTGCTGGCGTCCATCCTGGACACCGAGATTTCGCCCGAACTGGTGCCGGCGG
GCGAGGACCAGGCGCTGCAGAGCACGGAGGAACGGATCGGTCCCTACGCGCTGCAGGATTTCACGCTGTTCTATGTCCTG
CGCCACGGCTTCCGGCCCTCCCGCATCGCCTTCATGGCCGAAATCGCGTGGAAGGACGCCGGAATCGGGGCCTGGCCCCC
CGGTTTTCCGGCCGACCGGCGGGTGGAATACGACCTGCCGACCATCCGGCACTGGCTGTCGGTGTTCCTGACGCGCTTTT
TCGGGTTCAGCCAGTTCAAGCGCTCGGCGATGCCGAATGGGCCCAAGGTGGTGGCCGGCGGTGCCCTCTCGCCGCGTGGC
GACTGGCGCGCGCCCTCCGACGGCAATGCCAGGCTATGGCTGGAAGAGTTGGAAAGAAACGTCCCCTGA

Upstream 100 bases:

>100_bases
CTTGCCGATATAGGCGAAGTTCGACGCGATGCCGCCGAAGCCAAGTGCCGCCGACAGCAATGCGAGAACGAGGAAGATCA
GCGTCCAGCGTAACAGGTCC

Downstream 100 bases:

>100_bases
TCCCGCCCGTCGGCCTGCTCATGAACGCGCACGTGCCTCGGGGGCCGTCAGGCGAATCCAGGGATGGCGGTCTCGCAGCA
GCGTGACTACCGCGTCCTTG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MEISSSGSCVWTVRSMGVSMRGAPYGCVGGLPGQGAGSPAMDVKRAWSGDPLQPGDRIVADFRSLYRHGFARVAGCTLPV
ALADPATNVARMADMVRACHADGVALAVFPELGVSGYTIEDLRQQDVLLDGVGAALAALAAATADLVPVVVAGAPLRHGD
ALYNCAVILHRGTVLGVVPKSYLPNYREFYEARQFAPGAGLRGQTIHVAGQTAPFGTDLLFEAEDVPGLVIAIEICEDLW
VPAPPSTDAALAGATVIANPSASDITVGKADTRDLLCRSQSARALCAYVYAAAGEGESTTDLAWDGQVSVYENGALLAET
VRFPQGPNRATADIDLDLLRQERARMGSFADNRAARGLHATGGDTGWRRVGFALAPPPGDLGLMRRIERFPFVPADPARL
EQDCYEAWTIQVSALKQRLQATGTRRMVIGVSGGLDSTQALLVAVRAADELGLGRDAVLAYTMPGFGTSAGTQSNAMALM
QALGVTAAELDIRPTARMMLEQMGHPFASGVPQYDVTFENVQAGLRTDFLFRLANQHGGIVIGTGDLSELALGWCTYGVG
DQMSHYNVNAGLPKTLIQHLIRWVISARRVDDDAAGVLASILDTEISPELVPAGEDQALQSTEERIGPYALQDFTLFYVL
RHGFRPSRIAFMAEIAWKDAGIGAWPPGFPADRRVEYDLPTIRHWLSVFLTRFFGFSQFKRSAMPNGPKVVAGGALSPRG
DWRAPSDGNARLWLEELERNVP

Sequences:

>Translated_742_residues
MEISSSGSCVWTVRSMGVSMRGAPYGCVGGLPGQGAGSPAMDVKRAWSGDPLQPGDRIVADFRSLYRHGFARVAGCTLPV
ALADPATNVARMADMVRACHADGVALAVFPELGVSGYTIEDLRQQDVLLDGVGAALAALAAATADLVPVVVAGAPLRHGD
ALYNCAVILHRGTVLGVVPKSYLPNYREFYEARQFAPGAGLRGQTIHVAGQTAPFGTDLLFEAEDVPGLVIAIEICEDLW
VPAPPSTDAALAGATVIANPSASDITVGKADTRDLLCRSQSARALCAYVYAAAGEGESTTDLAWDGQVSVYENGALLAET
VRFPQGPNRATADIDLDLLRQERARMGSFADNRAARGLHATGGDTGWRRVGFALAPPPGDLGLMRRIERFPFVPADPARL
EQDCYEAWTIQVSALKQRLQATGTRRMVIGVSGGLDSTQALLVAVRAADELGLGRDAVLAYTMPGFGTSAGTQSNAMALM
QALGVTAAELDIRPTARMMLEQMGHPFASGVPQYDVTFENVQAGLRTDFLFRLANQHGGIVIGTGDLSELALGWCTYGVG
DQMSHYNVNAGLPKTLIQHLIRWVISARRVDDDAAGVLASILDTEISPELVPAGEDQALQSTEERIGPYALQDFTLFYVL
RHGFRPSRIAFMAEIAWKDAGIGAWPPGFPADRRVEYDLPTIRHWLSVFLTRFFGFSQFKRSAMPNGPKVVAGGALSPRG
DWRAPSDGNARLWLEELERNVP
>Mature_742_residues
MEISSSGSCVWTVRSMGVSMRGAPYGCVGGLPGQGAGSPAMDVKRAWSGDPLQPGDRIVADFRSLYRHGFARVAGCTLPV
ALADPATNVARMADMVRACHADGVALAVFPELGVSGYTIEDLRQQDVLLDGVGAALAALAAATADLVPVVVAGAPLRHGD
ALYNCAVILHRGTVLGVVPKSYLPNYREFYEARQFAPGAGLRGQTIHVAGQTAPFGTDLLFEAEDVPGLVIAIEICEDLW
VPAPPSTDAALAGATVIANPSASDITVGKADTRDLLCRSQSARALCAYVYAAAGEGESTTDLAWDGQVSVYENGALLAET
VRFPQGPNRATADIDLDLLRQERARMGSFADNRAARGLHATGGDTGWRRVGFALAPPPGDLGLMRRIERFPFVPADPARL
EQDCYEAWTIQVSALKQRLQATGTRRMVIGVSGGLDSTQALLVAVRAADELGLGRDAVLAYTMPGFGTSAGTQSNAMALM
QALGVTAAELDIRPTARMMLEQMGHPFASGVPQYDVTFENVQAGLRTDFLFRLANQHGGIVIGTGDLSELALGWCTYGVG
DQMSHYNVNAGLPKTLIQHLIRWVISARRVDDDAAGVLASILDTEISPELVPAGEDQALQSTEERIGPYALQDFTLFYVL
RHGFRPSRIAFMAEIAWKDAGIGAWPPGFPADRRVEYDLPTIRHWLSVFLTRFFGFSQFKRSAMPNGPKVVAGGALSPRG
DWRAPSDGNARLWLEELERNVP

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=615, Percent_Identity=26.6666666666667, Blast_Score=116, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17538756, Length=262, Percent_Identity=29.0076335877863, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321865, Length=665, Percent_Identity=22.5563909774436, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24641841, Length=614, Percent_Identity=24.2671009771987, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24641843, Length=614, Percent_Identity=24.2671009771987, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 79259; Mature: 79259

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISSSGSCVWTVRSMGVSMRGAPYGCVGGLPGQGAGSPAMDVKRAWSGDPLQPGDRIVA
CCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
DFRSLYRHGFARVAGCTLPVALADPATNVARMADMVRACHADGVALAVFPELGVSGYTIE
HHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
DLRQQDVLLDGVGAALAALAAATADLVPVVVAGAPLRHGDALYNCAVILHRGTVLGVVPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEECCCEEEECCH
SYLPNYREFYEARQFAPGAGLRGQTIHVAGQTAPFGTDLLFEAEDVPGLVIAIEICEDLW
HHCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCEEEEEEEHHHCC
VPAPPSTDAALAGATVIANPSASDITVGKADTRDLLCRSQSARALCAYVYAAAGEGESTT
CCCCCCCCHHHCCCEEEECCCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
DLAWDGQVSVYENGALLAETVRFPQGPNRATADIDLDLLRQERARMGSFADNRAARGLHA
CEEECCEEEEEECCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCC
TGGDTGWRRVGFALAPPPGDLGLMRRIERFPFVPADPARLEQDCYEAWTIQVSALKQRLQ
CCCCCCHHHCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEHHHHHHHHH
ATGTRRMVIGVSGGLDSTQALLVAVRAADELGLGRDAVLAYTMPGFGTSAGTQSNAMALM
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHH
QALGVTAAELDIRPTARMMLEQMGHPFASGVPQYDVTFENVQAGLRTDFLFRLANQHGGI
HHHCCCCEEECCCHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
VIGTGDLSELALGWCTYGVGDQMSHYNVNAGLPKTLIQHLIRWVISARRVDDDAAGVLAS
EEECCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILDTEISPELVPAGEDQALQSTEERIGPYALQDFTLFYVLRHGFRPSRIAFMAEIAWKDA
HHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GIGAWPPGFPADRRVEYDLPTIRHWLSVFLTRFFGFSQFKRSAMPNGPKVVAGGALSPRG
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEECCCCCCCC
DWRAPSDGNARLWLEELERNVP
CCCCCCCCCCEEEHHHHHCCCC
>Mature Secondary Structure
MEISSSGSCVWTVRSMGVSMRGAPYGCVGGLPGQGAGSPAMDVKRAWSGDPLQPGDRIVA
CCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
DFRSLYRHGFARVAGCTLPVALADPATNVARMADMVRACHADGVALAVFPELGVSGYTIE
HHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
DLRQQDVLLDGVGAALAALAAATADLVPVVVAGAPLRHGDALYNCAVILHRGTVLGVVPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEECCCEEEECCH
SYLPNYREFYEARQFAPGAGLRGQTIHVAGQTAPFGTDLLFEAEDVPGLVIAIEICEDLW
HHCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCEEEEEEEHHHCC
VPAPPSTDAALAGATVIANPSASDITVGKADTRDLLCRSQSARALCAYVYAAAGEGESTT
CCCCCCCCHHHCCCEEEECCCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
DLAWDGQVSVYENGALLAETVRFPQGPNRATADIDLDLLRQERARMGSFADNRAARGLHA
CEEECCEEEEEECCCEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCC
TGGDTGWRRVGFALAPPPGDLGLMRRIERFPFVPADPARLEQDCYEAWTIQVSALKQRLQ
CCCCCCHHHCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEHHHHHHHHH
ATGTRRMVIGVSGGLDSTQALLVAVRAADELGLGRDAVLAYTMPGFGTSAGTQSNAMALM
HCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHHH
QALGVTAAELDIRPTARMMLEQMGHPFASGVPQYDVTFENVQAGLRTDFLFRLANQHGGI
HHHCCCCEEECCCHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
VIGTGDLSELALGWCTYGVGDQMSHYNVNAGLPKTLIQHLIRWVISARRVDDDAAGVLAS
EEECCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILDTEISPELVPAGEDQALQSTEERIGPYALQDFTLFYVLRHGFRPSRIAFMAEIAWKDA
HHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
GIGAWPPGFPADRRVEYDLPTIRHWLSVFLTRFFGFSQFKRSAMPNGPKVVAGGALSPRG
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEECCCCCCCC
DWRAPSDGNARLWLEELERNVP
CCCCCCCCCCEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]