Click here to switch to the map view.

The map label for this gene is ykoW [H]

Identifier: 162146655

GI number: 162146655

Start: 851761

End: 853953

Strand: Direct

Name: ykoW [H]

Synonym: GDI_0833

Alternate gene names: 162146655

Gene position: 851761-853953 (Clockwise)

Preceding gene: 162146654

Following gene: 162146660

Centisome position: 21.6

GC content: 65.8

Gene sequence:

>2193_bases
GTGCGTTTGTGGTGCGTTCGTATCATCGTCGTGCCCACTGACAGAGTCGCGTCCGGCCGGCCGTTCCGGTCCTGCACCCC
ATCCGCCGAGGCCCGAATGCGACTTGTCGTCGTCGTTGATGACGGTTCCGCCACCCGGACGATCCTCGCGCGGATGGCCA
TGACGCTCGGCGCGGACATCCAGGTCGAAGCCTTCGCTTCCCCGATCGAGGCGCTGGACTGGTTTGGGGACACGAATATC
CCCGATCTGGTCATTGCCGATTGCAGAATGCCGATGATGGACGGGGCGGCTTTCACCGCGCGCATCCGCGCCCTGGGCTG
GGGCGGCGATGTGCCGGTGATGGTCGTGACGGCCCAGGAAGACCGCGAGGGTCGTATCAGCGCGCTGGCGGCCGGGGCCA
CCGATTTCCTGTCCAGCCCCGTCGATCACACGGAATTCCTGATCCGGGCCCGCAACCTGCTGCAACTGGGCTGGCACCAG
AAGCGCGCCCGCTCGCCCGTGCAACTGGAGCAGGAACTGGAAAACAGCCGGGCCGCGCAGGCCGAAATCCTGCGCGGCAG
CCGCGACCGCCTGCTGCAGGTCATCGACACGGTGCCCGCCCTGGTCAGCGCCACGGACGCCGAGGGGAATTACATCTTCG
TCAACAGATTCCATGCCCGGGTGATGGGGGCAGGGCAGGGGCGTAACGATGCGGCATCGCAGCTCCGCGACAGGCAGGTG
CTGGAAACCGGCCTGCCCACCGCGCGGTACGAGGAAAGCATTGCCGACCGGTACGGCATGGAACGTATCCTGCTGACGTC
CAAGATCCCGTTTTATGACGACGCCAGCGGTATGCGGTACGTCCTGACCACGTCGCTCGATATCACCGACCGGAAGAAGG
CCGAGCGGGAACTGCATCATCTGGCCTATTATGACCAGTTGACGGGTCTGCCCAGTCGCTCGAAGCTCGAGACCGAGATG
CAGGTGCTGCTGCGGCAGCGCGGGGCGGACGGCCCCCCTTTCGCGTTTGTGCTGATGGACCTCGACCGGTTCAAGGGCAT
CAACGACACGCAGGGCCATACGATCGGCGACCTGTTGCTGCGCAAGGTCGCGCTCCGGCTGCGGACGGCGCTGCCCCGGG
CCGGAATCATCGCGCGCCTGGGCGGGGACGAATTCGCCATCGTGCATGACGTCATCGACGCGGCGGACGCGGCGCACATG
GCGGAGCGGATCGTGGGGATCTTCGCCCGTCCCTTCACCCTGGGCGACCGGGTCACGAGCGTCGGCGCCAGTGCGGGGGT
GACGCTGTTCCCTGCCCGCGGCGCGACGTTCGAGACGCTGCTCAAGCGCGCGGATCTGGCGATGTACCGTGCCAAGAACG
AGGGACGCTGCCGCTACGCCTTTTTCGACGAGGAAATGGAACAGGCCTTCGCGGCCACGACCGAACTGGAGCAGGCATTG
CGCCGCGCGGTGGAGAACGAGGAATTCGTCCTGCATTATCAACCCCAGATCGATCTGGCCTCCGGCACCTTATGCGGGGT
CGAGGCCCTGATCCGCTGGGTGCAGCCGGGGGGCGACCTGGTCCATCCGGGCTGCTTCCTGCCGCTGGCCGAGGAAACCG
GGCTGATCGCGCCCATGACGGTGTGGTCCCTGCGCGAGGCCTGCCGCCAGGCGGCGCGATGGCAGAGCGACGGCATTCAT
ATCCGTGTCGCGGTCAACCTGTCGGGCGTCCTGTTTCGCCAGCATGACGTCCATCGACTGGTGGTGGACGCCACGACGGA
AGCCGGCCTGGACCCGTCCTTCCTTGAACTGGAACTGACCGAAACCGCGGTGATCGAAAACCCCGAATCCGCCGGGCGGC
AGCTTGCCGCCCTGCAGCAGATGGGCGTGTCGGTCGCGATAGACGATTTTGGAACCGGCTATGCCTCGCTGGTCTATCTG
ACCAGGCTTCCGATCCACCGCCTCAAGATCGATCGCGTCTTCATCCAGGACCTCGGTTCGTCACCGGGCAGCCGGGCCAT
TGTCAAGGCGATCATCGCGCTGGGCAAGAACCTGGGAATCCGCGTCATCGCCGAGGGGATCGAGCGCCAGGACCAGCTCC
GCTGGCTGGAAGAGCAGGACTGCCAAGAGGCCCAGGGCTTCTATTTCGGCTATCCTGTTCCACCGGCGAAACTTCAGGAC
CTGGTGGATAGCGGGACCCTGATGGTGCCTTAG

Upstream 100 bases:

>100_bases
CGGTGCCAGCCCGCAACTGGCCCTGGCCCTGGATGATCCCGGGAGTGTTCAGGCCGGGCCGTGACCGGAATCGGGCCGGA
ATGCATCCAAAACGAATATC

Downstream 100 bases:

>100_bases
TCGGCCTCTTAGAGTGCATGATTGGGTAAACCCAATCATGCACTCTAGATTTTTGTTTTCAGCGCATCTTCACGTGATTA
GGCGATTCCGCCTACTCAGG

Product: GGDEF family signaling protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MRLWCVRIIVVPTDRVASGRPFRSCTPSAEARMRLVVVVDDGSATRTILARMAMTLGADIQVEAFASPIEALDWFGDTNI
PDLVIADCRMPMMDGAAFTARIRALGWGGDVPVMVVTAQEDREGRISALAAGATDFLSSPVDHTEFLIRARNLLQLGWHQ
KRARSPVQLEQELENSRAAQAEILRGSRDRLLQVIDTVPALVSATDAEGNYIFVNRFHARVMGAGQGRNDAASQLRDRQV
LETGLPTARYEESIADRYGMERILLTSKIPFYDDASGMRYVLTTSLDITDRKKAERELHHLAYYDQLTGLPSRSKLETEM
QVLLRQRGADGPPFAFVLMDLDRFKGINDTQGHTIGDLLLRKVALRLRTALPRAGIIARLGGDEFAIVHDVIDAADAAHM
AERIVGIFARPFTLGDRVTSVGASAGVTLFPARGATFETLLKRADLAMYRAKNEGRCRYAFFDEEMEQAFAATTELEQAL
RRAVENEEFVLHYQPQIDLASGTLCGVEALIRWVQPGGDLVHPGCFLPLAEETGLIAPMTVWSLREACRQAARWQSDGIH
IRVAVNLSGVLFRQHDVHRLVVDATTEAGLDPSFLELELTETAVIENPESAGRQLAALQQMGVSVAIDDFGTGYASLVYL
TRLPIHRLKIDRVFIQDLGSSPGSRAIVKAIIALGKNLGIRVIAEGIERQDQLRWLEEQDCQEAQGFYFGYPVPPAKLQD
LVDSGTLMVP

Sequences:

>Translated_730_residues
MRLWCVRIIVVPTDRVASGRPFRSCTPSAEARMRLVVVVDDGSATRTILARMAMTLGADIQVEAFASPIEALDWFGDTNI
PDLVIADCRMPMMDGAAFTARIRALGWGGDVPVMVVTAQEDREGRISALAAGATDFLSSPVDHTEFLIRARNLLQLGWHQ
KRARSPVQLEQELENSRAAQAEILRGSRDRLLQVIDTVPALVSATDAEGNYIFVNRFHARVMGAGQGRNDAASQLRDRQV
LETGLPTARYEESIADRYGMERILLTSKIPFYDDASGMRYVLTTSLDITDRKKAERELHHLAYYDQLTGLPSRSKLETEM
QVLLRQRGADGPPFAFVLMDLDRFKGINDTQGHTIGDLLLRKVALRLRTALPRAGIIARLGGDEFAIVHDVIDAADAAHM
AERIVGIFARPFTLGDRVTSVGASAGVTLFPARGATFETLLKRADLAMYRAKNEGRCRYAFFDEEMEQAFAATTELEQAL
RRAVENEEFVLHYQPQIDLASGTLCGVEALIRWVQPGGDLVHPGCFLPLAEETGLIAPMTVWSLREACRQAARWQSDGIH
IRVAVNLSGVLFRQHDVHRLVVDATTEAGLDPSFLELELTETAVIENPESAGRQLAALQQMGVSVAIDDFGTGYASLVYL
TRLPIHRLKIDRVFIQDLGSSPGSRAIVKAIIALGKNLGIRVIAEGIERQDQLRWLEEQDCQEAQGFYFGYPVPPAKLQD
LVDSGTLMVP
>Mature_730_residues
MRLWCVRIIVVPTDRVASGRPFRSCTPSAEARMRLVVVVDDGSATRTILARMAMTLGADIQVEAFASPIEALDWFGDTNI
PDLVIADCRMPMMDGAAFTARIRALGWGGDVPVMVVTAQEDREGRISALAAGATDFLSSPVDHTEFLIRARNLLQLGWHQ
KRARSPVQLEQELENSRAAQAEILRGSRDRLLQVIDTVPALVSATDAEGNYIFVNRFHARVMGAGQGRNDAASQLRDRQV
LETGLPTARYEESIADRYGMERILLTSKIPFYDDASGMRYVLTTSLDITDRKKAERELHHLAYYDQLTGLPSRSKLETEM
QVLLRQRGADGPPFAFVLMDLDRFKGINDTQGHTIGDLLLRKVALRLRTALPRAGIIARLGGDEFAIVHDVIDAADAAHM
AERIVGIFARPFTLGDRVTSVGASAGVTLFPARGATFETLLKRADLAMYRAKNEGRCRYAFFDEEMEQAFAATTELEQAL
RRAVENEEFVLHYQPQIDLASGTLCGVEALIRWVQPGGDLVHPGCFLPLAEETGLIAPMTVWSLREACRQAARWQSDGIH
IRVAVNLSGVLFRQHDVHRLVVDATTEAGLDPSFLELELTETAVIENPESAGRQLAALQQMGVSVAIDDFGTGYASLVYL
TRLPIHRLKIDRVFIQDLGSSPGSRAIVKAIIALGKNLGIRVIAEGIERQDQLRWLEEQDCQEAQGFYFGYPVPPAKLQD
LVDSGTLMVP

Specific function: Probable signaling protein whose physiological role is not yet known [H]

COG id: COG5001

COG function: function code T; Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1787541, Length=540, Percent_Identity=32.7777777777778, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI87081921, Length=430, Percent_Identity=31.3953488372093, Blast_Score=235, Evalue=8e-63,
Organism=Escherichia coli, GI226510982, Length=448, Percent_Identity=30.8035714285714, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI87081845, Length=277, Percent_Identity=35.7400722021661, Blast_Score=157, Evalue=3e-39,
Organism=Escherichia coli, GI1790496, Length=239, Percent_Identity=33.0543933054393, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081743, Length=235, Percent_Identity=32.3404255319149, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1786507, Length=248, Percent_Identity=33.4677419354839, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI1788502, Length=233, Percent_Identity=30.9012875536481, Blast_Score=129, Evalue=9e-31,
Organism=Escherichia coli, GI1788381, Length=347, Percent_Identity=28.8184438040346, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081980, Length=260, Percent_Identity=31.1538461538462, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1787055, Length=287, Percent_Identity=27.5261324041812, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082096, Length=426, Percent_Identity=25.1173708920188, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788849, Length=258, Percent_Identity=29.8449612403101, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=182, Percent_Identity=32.967032967033, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081977, Length=156, Percent_Identity=35.2564102564103, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=34.9693251533742, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=32.9192546583851, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1788956, Length=155, Percent_Identity=34.8387096774194, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787410, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=33.7579617834395, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=33.125, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1786584, Length=176, Percent_Identity=31.25, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1787056, Length=167, Percent_Identity=29.3413173652695, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081974, Length=102, Percent_Identity=36.2745098039216, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 80614; Mature: 80614

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLWCVRIIVVPTDRVASGRPFRSCTPSAEARMRLVVVVDDGSATRTILARMAMTLGADI
CEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCE
QVEAFASPIEALDWFGDTNIPDLVIADCRMPMMDGAAFTARIRALGWGGDVPVMVVTAQE
EEHHHHCHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHEEEEEEEECCCCCCEEEEEECC
DREGRISALAAGATDFLSSPVDHTEFLIRARNLLQLGWHQKRARSPVQLEQELENSRAAQ
CCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHH
AEILRGSRDRLLQVIDTVPALVSATDAEGNYIFVNRFHARVMGAGQGRNDAASQLRDRQV
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHH
LETGLPTARYEESIADRYGMERILLTSKIPFYDDASGMRYVLTTSLDITDRKKAERELHH
HHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHH
LAYYDQLTGLPSRSKLETEMQVLLRQRGADGPPFAFVLMDLDRFKGINDTQGHTIGDLLL
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHCCCCCCCCCHHHHHHH
RKVALRLRTALPRAGIIARLGGDEFAIVHDVIDAADAAHMAERIVGIFARPFTLGDRVTS
HHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VGASAGVTLFPARGATFETLLKRADLAMYRAKNEGRCRYAFFDEEMEQAFAATTELEQAL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
RRAVENEEFVLHYQPQIDLASGTLCGVEALIRWVQPGGDLVHPGCFLPLAEETGLIAPMT
HHHHCCCCEEEEECCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHH
VWSLREACRQAARWQSDGIHIRVAVNLSGVLFRQHDVHRLVVDATTEAGLDPSFLELELT
HHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEECCCCEEEEEECCCCCCCCCCEEEEEEE
ETAVIENPESAGRQLAALQQMGVSVAIDDFGTGYASLVYLTRLPIHRLKIDRVFIQDLGS
HHHEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC
SPGSRAIVKAIIALGKNLGIRVIAEGIERQDQLRWLEEQDCQEAQGFYFGYPVPPAKLQD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHH
LVDSGTLMVP
HHCCCCEECC
>Mature Secondary Structure
MRLWCVRIIVVPTDRVASGRPFRSCTPSAEARMRLVVVVDDGSATRTILARMAMTLGADI
CEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCE
QVEAFASPIEALDWFGDTNIPDLVIADCRMPMMDGAAFTARIRALGWGGDVPVMVVTAQE
EEHHHHCHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHEEEEEEEECCCCCCEEEEEECC
DREGRISALAAGATDFLSSPVDHTEFLIRARNLLQLGWHQKRARSPVQLEQELENSRAAQ
CCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHH
AEILRGSRDRLLQVIDTVPALVSATDAEGNYIFVNRFHARVMGAGQGRNDAASQLRDRQV
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHH
LETGLPTARYEESIADRYGMERILLTSKIPFYDDASGMRYVLTTSLDITDRKKAERELHH
HHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHH
LAYYDQLTGLPSRSKLETEMQVLLRQRGADGPPFAFVLMDLDRFKGINDTQGHTIGDLLL
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHCCCCCCCCCHHHHHHH
RKVALRLRTALPRAGIIARLGGDEFAIVHDVIDAADAAHMAERIVGIFARPFTLGDRVTS
HHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VGASAGVTLFPARGATFETLLKRADLAMYRAKNEGRCRYAFFDEEMEQAFAATTELEQAL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
RRAVENEEFVLHYQPQIDLASGTLCGVEALIRWVQPGGDLVHPGCFLPLAEETGLIAPMT
HHHHCCCCEEEEECCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHH
VWSLREACRQAARWQSDGIHIRVAVNLSGVLFRQHDVHRLVVDATTEAGLDPSFLELELT
HHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEECCCCEEEEEECCCCCCCCCCEEEEEEE
ETAVIENPESAGRQLAALQQMGVSVAIDDFGTGYASLVYLTRLPIHRLKIDRVFIQDLGS
HHHEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC
SPGSRAIVKAIIALGKNLGIRVIAEGIERQDQLRWLEEQDCQEAQGFYFGYPVPPAKLQD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHH
LVDSGTLMVP
HHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710 [H]