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The map label for this gene is alkB [H]

Identifier: 162146641

GI number: 162146641

Start: 838267

End: 838944

Strand: Reverse

Name: alkB [H]

Synonym: GDI_0819

Alternate gene names: 162146641

Gene position: 838944-838267 (Counterclockwise)

Preceding gene: 162146642

Following gene: 162146640

Centisome position: 21.27

GC content: 70.8

Gene sequence:

>678_bases
ATGCCGCATCCATCCAGACCGCCGGACGCGGACACCCACCCCAGCCTGGAGTTGGACACGGGCGGGCGGGACCGCGTCCT
GGGGCCCGGCGCCATGGTGCTGGCGGGATTCGCCCGCGCCATCGCGCCCGACCTGATCGCCATGGTGGACATGGTCGCGC
GCACGGCGCCGTTTCGCCGCATGTCCACGCCCAGCGGCCGGACGATGTCGGTCGCCATGACCAATTGCGGCACCGCCGGA
TGGGTATCGGACACGAAGGGATACCGCTACGACCCGATGGACCCGCTATCGGGCCATCCGTGGCCCGCGCTGCCCGCCCT
GTTCGCCGACCTGGCGGCACGGGCCGCGTCCCGGGCCGGGTTTTCGGAATTCCACCCCGATGCCTGCCTGATCAACCGCT
ACGAGCCCGGAACGCGGCTGACCCTGCATCAGGACCGTGACGAGCACGATTTCAGCCAGCCGATCGTCTCGGTCTCGCTG
GGCCTGCCGGCCATCTTCCTGTGGGGCGGGCCGGCACGATCGGACCGGGTGCGGCGCGTGCCGCTGGAACATGGCGATGT
CGTGGTCTGGGGCGGCCCCGCGCGACTGGTCCATCACGGCATTCATCCGCTGACGGAGGGCATGCATCCGCTGACGGGCC
GGGCACGGCTGAACCTGACCTTCCGGCGGGCGATGTAG

Upstream 100 bases:

>100_bases
ATGGCTGGACGTCGTCCTGCTGCAACGCGAACTGGGCCCCGGGGACCGCTCGCCGCCACAGGCGCGGTCCTGACTGCCCC
CCCCATACGGTCTTCCCACC

Downstream 100 bases:

>100_bases
ATCGGCAGATGGCGGCGGGCGATGGACAGCGTGGCCGCCCGCGCCTATTCACGCGCGATGTCCCCGACCACCCCGACCCG
CATCGGCATCGATTTCGGCA

Product: alkylated DNA repair protein alkB

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MPHPSRPPDADTHPSLELDTGGRDRVLGPGAMVLAGFARAIAPDLIAMVDMVARTAPFRRMSTPSGRTMSVAMTNCGTAG
WVSDTKGYRYDPMDPLSGHPWPALPALFADLAARAASRAGFSEFHPDACLINRYEPGTRLTLHQDRDEHDFSQPIVSVSL
GLPAIFLWGGPARSDRVRRVPLEHGDVVVWGGPARLVHHGIHPLTEGMHPLTGRARLNLTFRRAM

Sequences:

>Translated_225_residues
MPHPSRPPDADTHPSLELDTGGRDRVLGPGAMVLAGFARAIAPDLIAMVDMVARTAPFRRMSTPSGRTMSVAMTNCGTAG
WVSDTKGYRYDPMDPLSGHPWPALPALFADLAARAASRAGFSEFHPDACLINRYEPGTRLTLHQDRDEHDFSQPIVSVSL
GLPAIFLWGGPARSDRVRRVPLEHGDVVVWGGPARLVHHGIHPLTEGMHPLTGRARLNLTFRRAM
>Mature_224_residues
PHPSRPPDADTHPSLELDTGGRDRVLGPGAMVLAGFARAIAPDLIAMVDMVARTAPFRRMSTPSGRTMSVAMTNCGTAGW
VSDTKGYRYDPMDPLSGHPWPALPALFADLAARAASRAGFSEFHPDACLINRYEPGTRLTLHQDRDEHDFSQPIVSVSLG
LPAIFLWGGPARSDRVRRVPLEHGDVVVWGGPARLVHHGIHPLTEGMHPLTGRARLNLTFRRAM

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=133, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788541, Length=198, Percent_Identity=58.5858585858586, Blast_Score=240, Evalue=4e-65,
Organism=Drosophila melanogaster, GI45555401, Length=166, Percent_Identity=27.1084337349398, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24490; Mature: 24359

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHPSRPPDADTHPSLELDTGGRDRVLGPGAMVLAGFARAIAPDLIAMVDMVARTAPFRR
CCCCCCCCCCCCCCCEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MSTPSGRTMSVAMTNCGTAGWVSDTKGYRYDPMDPLSGHPWPALPALFADLAARAASRAG
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
FSEFHPDACLINRYEPGTRLTLHQDRDEHDFSQPIVSVSLGLPAIFLWGGPARSDRVRRV
CHHHCCCEEEEECCCCCCEEEEECCCCCCHHHCCCEEEECCCCEEEEECCCCCCCCEEEC
PLEHGDVVVWGGPARLVHHGIHPLTEGMHPLTGRARLNLTFRRAM
CCCCCCEEEECCHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
PHPSRPPDADTHPSLELDTGGRDRVLGPGAMVLAGFARAIAPDLIAMVDMVARTAPFRR
CCCCCCCCCCCCCCEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
MSTPSGRTMSVAMTNCGTAGWVSDTKGYRYDPMDPLSGHPWPALPALFADLAARAASRAG
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
FSEFHPDACLINRYEPGTRLTLHQDRDEHDFSQPIVSVSLGLPAIFLWGGPARSDRVRRV
CHHHCCCEEEEECCCCCCEEEEECCCCCCHHHCCCEEEECCCCEEEEECCCCCCCCEEEC
PLEHGDVVVWGGPARLVHHGIHPLTEGMHPLTGRARLNLTFRRAM
CCCCCCEEEECCHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]