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The map label for this gene is thiED [H]

Identifier: 162146635

GI number: 162146635

Start: 832349

End: 833158

Strand: Direct

Name: thiED [H]

Synonym: GDI_0813

Alternate gene names: 162146635

Gene position: 832349-833158 (Clockwise)

Preceding gene: 162146634

Following gene: 162146636

Centisome position: 21.1

GC content: 70.86

Gene sequence:

>810_bases
ATGAAGGGGCGCATCCTCGTCATCGCGGGCAGCGATTCCGGCGGCGGCGCGGGAATCCAGGCGGATATCAAGACCATCAC
GGCGCTGGGCGGGTTTGCCATGACGGCGCTGTCCGCCCTGACCGTGCAGAACACGCTGGGGGTGACGGGCGTGCTGCCGG
TCCCGCCCGATTTCGTGGCGCAGCAGATGTGCAGCGTGCTGGACGACCTGGGCGCCGATGCGTTCAAGAGCGGGATGCTG
GACCGGGCAGAGGTCATCCTGGCCGTCGCGGACATCATTCGTGTCCATCCCGGCATCCCCTATGTCCTCGATCCCGTCAT
GGTGGCCAAGGGCGGCTCGCGCCTGTTGCAGGATAACGCGGTGGAGACGCTCAGGCGGGATCTCCTGCCTCTGGCCACCC
TGCTGACCCCCAACCTGCCGGAGGCCGAAGTCCTGGTCGGACGTCCGATTCGCACGGTGGACGACATGCGGCGGGCGGCC
GAGGACCTGCGCCGGGCCGGGGCCGGGGCGGTCCTGGTCAAGGGCGGGCACCTGGATGGCGACGTGCTGACCGACGTGCT
GGCCACCGACGCGGGGATCGAGCTGTTTTCGGATACGCGGCTGCATACCCGCCACACCCATGGGACAGGCTGCACGCTGG
CCAGCGCGATCGCCACCGGCCTGGCGCAGGGCCTGACGCTGCGTGATGCCGTGATCCGGGCCCGGCGCTACCTGCGCGCC
GCCATCGCGGCCGCGCCGGGCTATGGCGCGGGCGCCGGACCCCTGAACCATGCGGTCACGATCGGCCCCGACTGGACCCC
CGCCGCCTGA

Upstream 100 bases:

>100_bases
TCATGGCCGAGGCCGAGGACCCGGCGCTGGTGGAACGAATCGTCGCCGAAATGTGTGAGGCGATCGATTCGGCCCAGGTC
GTGGCCTAGGGACGGGGGCC

Downstream 100 bases:

>100_bases
TCACGCTTCGTCCCGTCGACCGGATGGACGGCCGGGCTGCGCCCAGTCGGCGGCGTCGGCCATGCGCACGATGACGGGAA
CCAGGCGCGAGACGTGATTC

Product: bifunctional thiED protein

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKGRILVIAGSDSGGGAGIQADIKTITALGGFAMTALSALTVQNTLGVTGVLPVPPDFVAQQMCSVLDDLGADAFKSGML
DRAEVILAVADIIRVHPGIPYVLDPVMVAKGGSRLLQDNAVETLRRDLLPLATLLTPNLPEAEVLVGRPIRTVDDMRRAA
EDLRRAGAGAVLVKGGHLDGDVLTDVLATDAGIELFSDTRLHTRHTHGTGCTLASAIATGLAQGLTLRDAVIRARRYLRA
AIAAAPGYGAGAGPLNHAVTIGPDWTPAA

Sequences:

>Translated_269_residues
MKGRILVIAGSDSGGGAGIQADIKTITALGGFAMTALSALTVQNTLGVTGVLPVPPDFVAQQMCSVLDDLGADAFKSGML
DRAEVILAVADIIRVHPGIPYVLDPVMVAKGGSRLLQDNAVETLRRDLLPLATLLTPNLPEAEVLVGRPIRTVDDMRRAA
EDLRRAGAGAVLVKGGHLDGDVLTDVLATDAGIELFSDTRLHTRHTHGTGCTLASAIATGLAQGLTLRDAVIRARRYLRA
AIAAAPGYGAGAGPLNHAVTIGPDWTPAA
>Mature_269_residues
MKGRILVIAGSDSGGGAGIQADIKTITALGGFAMTALSALTVQNTLGVTGVLPVPPDFVAQQMCSVLDDLGADAFKSGML
DRAEVILAVADIIRVHPGIPYVLDPVMVAKGGSRLLQDNAVETLRRDLLPLATLLTPNLPEAEVLVGRPIRTVDDMRRAA
EDLRRAGAGAVLVKGGHLDGDVLTDVLATDAGIELFSDTRLHTRHTHGTGCTLASAIATGLAQGLTLRDAVIRARRYLRA
AIAAAPGYGAGAGPLNHAVTIGPDWTPAA

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=255, Percent_Identity=45.0980392156863, Blast_Score=196, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6325378, Length=271, Percent_Identity=35.7933579335793, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324997, Length=272, Percent_Identity=37.1323529411765, Blast_Score=133, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=36.3970588235294, Blast_Score=133, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27740; Mature: 27740

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRILVIAGSDSGGGAGIQADIKTITALGGFAMTALSALTVQNTLGVTGVLPVPPDFVA
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHH
QQMCSVLDDLGADAFKSGMLDRAEVILAVADIIRVHPGIPYVLDPVMVAKGGSRLLQDNA
HHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHCCCCHHHHHHH
VETLRRDLLPLATLLTPNLPEAEVLVGRPIRTVDDMRRAAEDLRRAGAGAVLVKGGHLDG
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
DVLTDVLATDAGIELFSDTRLHTRHTHGTGCTLASAIATGLAQGLTLRDAVIRARRYLRA
HHHHHHHHHCCCCHHHCCCCCEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AIAAAPGYGAGAGPLNHAVTIGPDWTPAA
HHHHCCCCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MKGRILVIAGSDSGGGAGIQADIKTITALGGFAMTALSALTVQNTLGVTGVLPVPPDFVA
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHH
QQMCSVLDDLGADAFKSGMLDRAEVILAVADIIRVHPGIPYVLDPVMVAKGGSRLLQDNA
HHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCHHHHHCCCCHHHHHHH
VETLRRDLLPLATLLTPNLPEAEVLVGRPIRTVDDMRRAAEDLRRAGAGAVLVKGGHLDG
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCH
DVLTDVLATDAGIELFSDTRLHTRHTHGTGCTLASAIATGLAQGLTLRDAVIRARRYLRA
HHHHHHHHHCCCCHHHCCCCCEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AIAAAPGYGAGAGPLNHAVTIGPDWTPAA
HHHHCCCCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA