Click here to switch to the map view.

The map label for this gene is glcF [H]

Identifier: 162146569

GI number: 162146569

Start: 764355

End: 765668

Strand: Direct

Name: glcF [H]

Synonym: GDI_0746

Alternate gene names: 162146569

Gene position: 764355-765668 (Clockwise)

Preceding gene: 162146568

Following gene: 162146571

Centisome position: 19.38

GC content: 70.55

Gene sequence:

>1314_bases
ATGCTGAATACCATCTCCCCGGCCGCACGCCGGGATTCCGATATCGCGCTGGCCGCCGACATCATCGATGCCTGCGTCCA
TTGCGGCATCTGCCTGTCGCATTGTCCGACCTATCAGGTGCGGCACGAGGAAAATGACAGCCCGCGCGGGCGGATTTTCC
TGATGAAGGACATGTATGAAAAAGGCGGCGCCCCGGACGCGCGCACCGTCCTGCATCTGGACCGCTGCCTGACCTGCCTG
GCATGCGAGGCGATCTGCCCGTCCGGCGTCGAATATTCCAAACTGATCGGCCCCGGCCGCGCCTATGTCGCGTCCCATTT
CCGCCGTCCGCCAGTCCAGGCGGCGATCCGCCGCATGCTGGTCCTGCTGCTGGCGCGGCCCCGCCTGTTCCGCACGGCGC
TGGAGGCCGGGCGGCTGGGGCGCCCGCTGCGTGCCCTGCTGTCAGGCCCAGTGCGCGCGATGCTCGACAAGGTGCCGGCC
GCGCCGCTGCCGCCGCCCAGCCCCGTCGACCGGCCGCAGGTCTTCCCGGCCCAGGGACCGCGCCGCATGAGGGTCGCGTT
GCTGAACGGCTGCGTGCAGACCGTCCTGGACACCGCGATCAACGAGGCGACGATCCGCCTGCTCCGCCGCCATGGGGTCG
AGGTGGTGGTGGCGCGCGGCGCGGGGTGCTGCGGCGCGCTGGCCGAGCATATGGGCGACGAGGCCCCGGCCCTGAAGCGG
GCGCGGGCGAACATCGCCGCCTGGCTGCGCGAGGCCGACGGGCCGCACGGCCTGGACGCGGTCGTCATCAACGCCTCGGG
CTGCGGCAATTCGGTCAAGGCCTACGGCCATGCCCTGCGGAACGATCCGGCCTGGGCGCCGCGCGCGGCGCGTATCAGCG
CCATGACGCTGGATATCAGCGAAATCATGGACCGGATCGGCCTGATGAAGCCGGTCGTCGCGCCCGGGCTGCGGGTGGTC
TATCACGCCGCCTGTTCGCTGCAGCACGCGCAGCGCGTCACGCGGCAGCCGAAATCCCTGCTGCAGGCCGCCGGCTTCAC
GGTGCTGGACGTGCCCGAAGGGTATCTGTGCTGCGGGTCGGCCGGGACCTACAACATGTTGCAGCCGGGCATCGCCGAGG
AGTTGAAGCGGCGCAAGGTCGCGGCCATCCGTTCGGTCGACGGGCAGGTCGCGGCCAGCGGCAATATCGGCTGCATCACG
CACCTGGCCGCCGATGCCGGCCTGCCCTTCGTCCACACGGTGCAGCTCCTGGACTGGGCCACGGGCGGGCCCCGCCCGGA
TGGACTTCCGGAGACCGCTTCAAACAGCCTCTGA

Upstream 100 bases:

>100_bases
CGGCGGTGCGCGCGCTGTCCGAACGGACGCGCCGCGTCTTCGATCCGGCCGGAATCCTCAATCCCGGCCGCATGACCCCG
CCGGCGCAGGAGGACGCGGC

Downstream 100 bases:

>100_bases
GCGGAACCGACCGATCATTCCGACGACGGCTGATCGCCGATCTTCGTATTGTTGATGCGGTCCCAGGCCCCCCGGCTGGC
CATGCAGGCGGCGTAGGTAT

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MLNTISPAARRDSDIALAADIIDACVHCGICLSHCPTYQVRHEENDSPRGRIFLMKDMYEKGGAPDARTVLHLDRCLTCL
ACEAICPSGVEYSKLIGPGRAYVASHFRRPPVQAAIRRMLVLLLARPRLFRTALEAGRLGRPLRALLSGPVRAMLDKVPA
APLPPPSPVDRPQVFPAQGPRRMRVALLNGCVQTVLDTAINEATIRLLRRHGVEVVVARGAGCCGALAEHMGDEAPALKR
ARANIAAWLREADGPHGLDAVVINASGCGNSVKAYGHALRNDPAWAPRAARISAMTLDISEIMDRIGLMKPVVAPGLRVV
YHAACSLQHAQRVTRQPKSLLQAAGFTVLDVPEGYLCCGSAGTYNMLQPGIAEELKRRKVAAIRSVDGQVAASGNIGCIT
HLAADAGLPFVHTVQLLDWATGGPRPDGLPETASNSL

Sequences:

>Translated_437_residues
MLNTISPAARRDSDIALAADIIDACVHCGICLSHCPTYQVRHEENDSPRGRIFLMKDMYEKGGAPDARTVLHLDRCLTCL
ACEAICPSGVEYSKLIGPGRAYVASHFRRPPVQAAIRRMLVLLLARPRLFRTALEAGRLGRPLRALLSGPVRAMLDKVPA
APLPPPSPVDRPQVFPAQGPRRMRVALLNGCVQTVLDTAINEATIRLLRRHGVEVVVARGAGCCGALAEHMGDEAPALKR
ARANIAAWLREADGPHGLDAVVINASGCGNSVKAYGHALRNDPAWAPRAARISAMTLDISEIMDRIGLMKPVVAPGLRVV
YHAACSLQHAQRVTRQPKSLLQAAGFTVLDVPEGYLCCGSAGTYNMLQPGIAEELKRRKVAAIRSVDGQVAASGNIGCIT
HLAADAGLPFVHTVQLLDWATGGPRPDGLPETASNSL
>Mature_437_residues
MLNTISPAARRDSDIALAADIIDACVHCGICLSHCPTYQVRHEENDSPRGRIFLMKDMYEKGGAPDARTVLHLDRCLTCL
ACEAICPSGVEYSKLIGPGRAYVASHFRRPPVQAAIRRMLVLLLARPRLFRTALEAGRLGRPLRALLSGPVRAMLDKVPA
APLPPPSPVDRPQVFPAQGPRRMRVALLNGCVQTVLDTAINEATIRLLRRHGVEVVVARGAGCCGALAEHMGDEAPALKR
ARANIAAWLREADGPHGLDAVVINASGCGNSVKAYGHALRNDPAWAPRAARISAMTLDISEIMDRIGLMKPVVAPGLRVV
YHAACSLQHAQRVTRQPKSLLQAAGFTVLDVPEGYLCCGSAGTYNMLQPGIAEELKRRKVAAIRSVDGQVAASGNIGCIT
HLAADAGLPFVHTVQLLDWATGGPRPDGLPETASNSL

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=422, Percent_Identity=37.914691943128, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1786497, Length=226, Percent_Identity=28.3185840707965, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788576, Length=358, Percent_Identity=24.5810055865922, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46709; Mature: 46709

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTISPAARRDSDIALAADIIDACVHCGICLSHCPTYQVRHEENDSPRGRIFLMKDMYE
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEHHHHH
KGGAPDARTVLHLDRCLTCLACEAICPSGVEYSKLIGPGRAYVASHFRRPPVQAAIRRML
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHCCCCCHHHHHHHHH
VLLLARPRLFRTALEAGRLGRPLRALLSGPVRAMLDKVPAAPLPPPSPVDRPQVFPAQGP
HHHHHCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
RRMRVALLNGCVQTVLDTAINEATIRLLRRHGVEVVVARGAGCCGALAEHMGDEAPALKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHH
ARANIAAWLREADGPHGLDAVVINASGCGNSVKAYGHALRNDPAWAPRAARISAMTLDIS
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHEEEEECHH
EIMDRIGLMKPVVAPGLRVVYHAACSLQHAQRVTRQPKSLLQAAGFTVLDVPEGYLCCGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECC
AGTYNMLQPGIAEELKRRKVAAIRSVDGQVAASGNIGCITHLAADAGLPFVHTVQLLDWA
CCCCHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCCHHHHHHHHHHC
TGGPRPDGLPETASNSL
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLNTISPAARRDSDIALAADIIDACVHCGICLSHCPTYQVRHEENDSPRGRIFLMKDMYE
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEHHHHH
KGGAPDARTVLHLDRCLTCLACEAICPSGVEYSKLIGPGRAYVASHFRRPPVQAAIRRML
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHCCCCCHHHHHHHHH
VLLLARPRLFRTALEAGRLGRPLRALLSGPVRAMLDKVPAAPLPPPSPVDRPQVFPAQGP
HHHHHCCHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
RRMRVALLNGCVQTVLDTAINEATIRLLRRHGVEVVVARGAGCCGALAEHMGDEAPALKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCHHHHH
ARANIAAWLREADGPHGLDAVVINASGCGNSVKAYGHALRNDPAWAPRAARISAMTLDIS
HHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHEEEEECHH
EIMDRIGLMKPVVAPGLRVVYHAACSLQHAQRVTRQPKSLLQAAGFTVLDVPEGYLCCGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECC
AGTYNMLQPGIAEELKRRKVAAIRSVDGQVAASGNIGCITHLAADAGLPFVHTVQLLDWA
CCCCHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCCHHHHHHHHHHC
TGGPRPDGLPETASNSL
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]