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The map label for this gene is drgA [H]

Identifier: 162146420

GI number: 162146420

Start: 601555

End: 602202

Strand: Reverse

Name: drgA [H]

Synonym: GDI_0595

Alternate gene names: 162146420

Gene position: 602202-601555 (Counterclockwise)

Preceding gene: 162146421

Following gene: 162146406

Centisome position: 15.27

GC content: 61.73

Gene sequence:

>648_bases
ATGATGACTGACGGAACAAAGGAGAACCCCGTAATGACCCTTGATGTTGCCAGCGCCATTCTCAAACGCCGGGCCACCAA
GAATTTCGATGCCGGCCACCGCATGCCCGCCGGCGATCTTGATGCCATTCTCGGCCTCGCCCGCCGGATGCCGACGGCCT
TCAACATCCAGAACTGGCGTTTCGTGGCCGTCAGCGATCCGAAACTGCGCCAGGATATCCGCAAGGTCGCGTGGGACCAG
CCGCAGGTGACGGATGCGTCGGTCCTGCTGGTCCTGTGCGCCGATATCAAGGCCTGGGAAAAATACCCCGCGCGCTACTG
GGAAAATGCGGATTCATCCGTACGCGATTTCATGGTGGGGGCGATCGACCAGTATTATCGCGGACGCGAAGACGTCCAGC
GTGACGAGGGCATGCGGTCCTGCGGCATGGCGGCAGGTGCGATCATGCTGCTGGCCGAAAGCATGGGCTACGCCACCTGC
CCGATGGACGGTTTCGATTTCGCGGCCGTCGGCAAGCTGATCAAGCTGCCGGCGGACCACGAGATCGTCATGTTCGTGGC
CGTGGGAAAGCCCGCGTCCGAGCCCTATCCCACGGGCGGCATCCTGCCGGCCGACGAGATTATTTTCGAAAACACGTTTG
GGGCGTAA

Upstream 100 bases:

>100_bases
GTCCAGCTTGATCGCACCGCCACGTTCCAGCGCGACGGCGTGTCGCGTGTCAGGCTGCCGGTGAGCATCTTCACGCTCGA
CGCCCACCTGCCCCCCGCCC

Downstream 100 bases:

>100_bases
CCGGACGATCCGGTCACGCGGGAGGCGGGGCGGCGCCGATCTCCAGCGCCAGGCGCGCCATCCTGATCGCGCCGTCCCGG
CTTCCCGCACCACAGATGAA

Product: NAD(P)H nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MMTDGTKENPVMTLDVASAILKRRATKNFDAGHRMPAGDLDAILGLARRMPTAFNIQNWRFVAVSDPKLRQDIRKVAWDQ
PQVTDASVLLVLCADIKAWEKYPARYWENADSSVRDFMVGAIDQYYRGREDVQRDEGMRSCGMAAGAIMLLAESMGYATC
PMDGFDFAAVGKLIKLPADHEIVMFVAVGKPASEPYPTGGILPADEIIFENTFGA

Sequences:

>Translated_215_residues
MMTDGTKENPVMTLDVASAILKRRATKNFDAGHRMPAGDLDAILGLARRMPTAFNIQNWRFVAVSDPKLRQDIRKVAWDQ
PQVTDASVLLVLCADIKAWEKYPARYWENADSSVRDFMVGAIDQYYRGREDVQRDEGMRSCGMAAGAIMLLAESMGYATC
PMDGFDFAAVGKLIKLPADHEIVMFVAVGKPASEPYPTGGILPADEIIFENTFGA
>Mature_215_residues
MMTDGTKENPVMTLDVASAILKRRATKNFDAGHRMPAGDLDAILGLARRMPTAFNIQNWRFVAVSDPKLRQDIRKVAWDQ
PQVTDASVLLVLCADIKAWEKYPARYWENADSSVRDFMVGAIDQYYRGREDVQRDEGMRSCGMAAGAIMLLAESMGYATC
PMDGFDFAAVGKLIKLPADHEIVMFVAVGKPASEPYPTGGILPADEIIFENTFGA

Specific function: Controls resistance to the herbicide Dinoseb and metronidazole. Involved in detoxification of Dinoseb via the reduction of the nitro group(s) and this process is accompanied by the formation of toxic superoxide anions [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23729; Mature: 23729

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTDGTKENPVMTLDVASAILKRRATKNFDAGHRMPAGDLDAILGLARRMPTAFNIQNWR
CCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCEE
FVAVSDPKLRQDIRKVAWDQPQVTDASVLLVLCADIKAWEKYPARYWENADSSVRDFMVG
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AIDQYYRGREDVQRDEGMRSCGMAAGAIMLLAESMGYATCPMDGFDFAAVGKLIKLPADH
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHCCCCCC
EIVMFVAVGKPASEPYPTGGILPADEIIFENTFGA
CEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MMTDGTKENPVMTLDVASAILKRRATKNFDAGHRMPAGDLDAILGLARRMPTAFNIQNWR
CCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCEE
FVAVSDPKLRQDIRKVAWDQPQVTDASVLLVLCADIKAWEKYPARYWENADSSVRDFMVG
EEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AIDQYYRGREDVQRDEGMRSCGMAAGAIMLLAESMGYATCPMDGFDFAAVGKLIKLPADH
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHCCCCCC
EIVMFVAVGKPASEPYPTGGILPADEIIFENTFGA
CEEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9654132; 8905231; 9298645 [H]