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The map label for this gene is cysU [H]

Identifier: 162146384

GI number: 162146384

Start: 565330

End: 566175

Strand: Direct

Name: cysU [H]

Synonym: GDI_0559

Alternate gene names: 162146384

Gene position: 565330-566175 (Clockwise)

Preceding gene: 162146383

Following gene: 162146385

Centisome position: 14.33

GC content: 72.22

Gene sequence:

>846_bases
ATGGCGTTTCGCCTGCGGCGGCGCGACGCGCTGCCGGGTTTTTCGGTCTCGATCGGCTTCACGCTGGTCTGGATCGGGCT
GATCGTGGCGCTGCCCCTGGTGGCGCTGGTGCTTCGTCCCTGGCAGGACGGCACGGGGGCCGTGCTGCGGGCGCTGCATG
ATCCGCGCATATACGCCGCCCTGCGCGTCAGCTTCGGCTGCGCGGCGCTGGCCGCGTTGCTCGACGTGCCGATGGGACTG
CTTCTGGCATGGGCGCTGGTCCGGGGGCGGGTGCCCGGGCGGCGGGCGATCGACGCGCTGATCGACCTGCCCTTCGCCCT
GCCCACCGCCGTGACGGGCATCACGCTGGCCACGCTGTACGGACCCAACGGCTGGCTGGGCGCACCCCTGGCCAGGGCGG
GCATCCATATCGCCTTTACGCCGGCCGGCATCGTCACCGCCCTGGTCTTCGTCGGCCTGCCCTTCGTCGTCCGCGCGGTC
GAGCCCGTCCTGCGGGCCCTGCCGCCCGAGATGGAGGAAGCCTCGATGCTGCTGGGCGCCAGTCCGTTCCAGACGGTCCG
GCACGTGCTGCTGCCGCCCCTGCTGCCGCCGGTCATCGGCGGCTTCAGCCTGGCCTTCGCCCGCTGCATCGGGGAATACG
GCTCGGTCATCTTCATCGCCGGCAACCAGCCCTTCCGCAGCGAGATCGCGCCGCTGCTGATCGTGGTACGCCTGCAGGAA
TTCGACTACAGCGGCGCCACCGCCATCGCGCTGATCCTGCTGGTCAGCGCGCTGCTGTGCCAGACCGGCGTGTCCCGGCT
GCGGCGGCGGGCGTCGCGCGGGCTCGTCGCCCCGGATGCCGCCTGA

Upstream 100 bases:

>100_bases
TCGACGTCGCCAGCCTGGGCGGATGGACCCGCGTGCAGAAGACGCATTTCGCCGACGGCGGCGTGTTCGACCAGATCTAC
AGCCGGTAACGGGTTCCGTC

Downstream 100 bases:

>100_bases
TGCCCGGCCGGTCCTCCCGCACGGGCGGCGTCCTGCTGGCGGCTGCGGTGTACGCCGTGGTCCTGCTGGTGCTGGTCCTT
CCGCTGGTGCTGGTCGTCGC

Product: sulfate ABC transporter permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MAFRLRRRDALPGFSVSIGFTLVWIGLIVALPLVALVLRPWQDGTGAVLRALHDPRIYAALRVSFGCAALAALLDVPMGL
LLAWALVRGRVPGRRAIDALIDLPFALPTAVTGITLATLYGPNGWLGAPLARAGIHIAFTPAGIVTALVFVGLPFVVRAV
EPVLRALPPEMEEASMLLGASPFQTVRHVLLPPLLPPVIGGFSLAFARCIGEYGSVIFIAGNQPFRSEIAPLLIVVRLQE
FDYSGATAIALILLVSALLCQTGVSRLRRRASRGLVAPDAA

Sequences:

>Translated_281_residues
MAFRLRRRDALPGFSVSIGFTLVWIGLIVALPLVALVLRPWQDGTGAVLRALHDPRIYAALRVSFGCAALAALLDVPMGL
LLAWALVRGRVPGRRAIDALIDLPFALPTAVTGITLATLYGPNGWLGAPLARAGIHIAFTPAGIVTALVFVGLPFVVRAV
EPVLRALPPEMEEASMLLGASPFQTVRHVLLPPLLPPVIGGFSLAFARCIGEYGSVIFIAGNQPFRSEIAPLLIVVRLQE
FDYSGATAIALILLVSALLCQTGVSRLRRRASRGLVAPDAA
>Mature_280_residues
AFRLRRRDALPGFSVSIGFTLVWIGLIVALPLVALVLRPWQDGTGAVLRALHDPRIYAALRVSFGCAALAALLDVPMGLL
LAWALVRGRVPGRRAIDALIDLPFALPTAVTGITLATLYGPNGWLGAPLARAGIHIAFTPAGIVTALVFVGLPFVVRAVE
PVLRALPPEMEEASMLLGASPFQTVRHVLLPPLLPPVIGGFSLAFARCIGEYGSVIFIAGNQPFRSEIAPLLIVVRLQEF
DYSGATAIALILLVSALLCQTGVSRLRRRASRGLVAPDAA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=274, Percent_Identity=45.2554744525547, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI87082099, Length=282, Percent_Identity=32.2695035460993, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786980, Length=228, Percent_Identity=33.7719298245614, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786254, Length=240, Percent_Identity=31.6666666666667, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29814; Mature: 29683

Theoretical pI: Translated: 11.25; Mature: 11.25

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFRLRRRDALPGFSVSIGFTLVWIGLIVALPLVALVLRPWQDGTGAVLRALHDPRIYAA
CCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
LRVSFGCAALAALLDVPMGLLLAWALVRGRVPGRRAIDALIDLPFALPTAVTGITLATLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHEEE
GPNGWLGAPLARAGIHIAFTPAGIVTALVFVGLPFVVRAVEPVLRALPPEMEEASMLLGA
CCCCCCCCCHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
SPFQTVRHVLLPPLLPPVIGGFSLAFARCIGEYGSVIFIAGNQPFRSEIAPLLIVVRLQE
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHH
FDYSGATAIALILLVSALLCQTGVSRLRRRASRGLVAPDAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AFRLRRRDALPGFSVSIGFTLVWIGLIVALPLVALVLRPWQDGTGAVLRALHDPRIYAA
CCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
LRVSFGCAALAALLDVPMGLLLAWALVRGRVPGRRAIDALIDLPFALPTAVTGITLATLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHEEE
GPNGWLGAPLARAGIHIAFTPAGIVTALVFVGLPFVVRAVEPVLRALPPEMEEASMLLGA
CCCCCCCCCHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
SPFQTVRHVLLPPLLPPVIGGFSLAFARCIGEYGSVIFIAGNQPFRSEIAPLLIVVRLQE
CHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHH
FDYSGATAIALILLVSALLCQTGVSRLRRRASRGLVAPDAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]