Click here to switch to the map view.

The map label for this gene is glgX [H]

Identifier: 162146370

GI number: 162146370

Start: 542035

End: 544161

Strand: Direct

Name: glgX [H]

Synonym: GDI_0545

Alternate gene names: 162146370

Gene position: 542035-544161 (Clockwise)

Preceding gene: 162146369

Following gene: 162146371

Centisome position: 13.74

GC content: 68.22

Gene sequence:

>2127_bases
ATGATGCGCTTCCCCACCCGGCTGATGCCGGGGGCGGACACGCCGCTGGGTGCGAGCTGGGACGGGCTGGGCGTCAATTT
CGCGGTCTTCTCCGCCAACGCGCTGCGGATCGACCTGTGCATCTTCGATCCCTCGGGCCGGCGGGAAATCGCGCGGTTCG
AACTGCCCGAACGCACCGATGAAGTCTGGCACGGCTACCTGCCCGACGCGCGGCCCGGACTGTTGTACGGCTATCGCGCC
TATGGTCCGTACGAACCGCTGCGCGGTCATCGCTTCAATCCGCACAAGCTGCTGGTCGATCCCTATGCCCGCGCGCTGCA
CGGGCAACTGAGCTGGTCGGATTCGCTGTTCGGCTACCGCATCAGCTCGCCGCGCGGCGACCTGTCGATCGACCGGCGCG
ACAGTGCCCCCGCCATGCCGAAAAGCGTCGTGACCAACGACGCCTTCAACTGGGGCGACGACCGGCTGCCGCGCGTGCCG
TGGGACCGCACCGTGATCATGGAAGCCCACCTGCGCGGGCTGTCGATGCGCCGCAGCGAACTGACCCCGGTCGAACGCGG
TACCTTCCGCGCCCTGGCCGACCCGCAACTGATCGACCATCTGCTGCGCCTGGGCATCACCACGCTGGAACTGATGCCCG
TCCACGCCTTCGTCAAGGACCGGTTCCTGCTGGAGAGGGGCCTGACGAACTACTGGGGCTACAATACGCTGGCCTTCTTC
GCGCCCCATGCCGGCTACCTGGCCGAAGGCTCGCCGCACGAGGTCCGCACCGCCATCCGCCGCCTGCACGCGGCGGGGAT
CGAAGTGGTGCTGGACGTCGTCTACAATCATACGTGCGAAGGCAACGAACTGGGTCCCACCCTGTGCTATCGCGGCCTGG
ACAACGCCGTCTATTACCGTCTGGTGCCCGAGGACGAGCGGCACCTGATCAACGATACCGGCTGCGGCAACACGCTGAAC
CTGTCCCATCCCAGGGTCTTGCAGATGGTCATGGATTCGCTGCGGCACTGGGCGGTCGATTTCCATGTCGACGGCTTCCG
CTTCGACCTGTGTTCCACCCTGGGGCGCGAAAGCTATGGCTTCGACCAGGCCTGCGGCTTCTTCGACGTGCTGCGCCAGG
ACCCGGTCCTGTCGAAGCTGAAACTGATCGCCGAACCCTGGGACCCGGGGCCGGGCGGCTATCAGCTGGGCAACCATCCG
CCGGGTTTCGCCGAATGGAACGACCGGTTCCGCGACACGGTGCGGCGGTTCTGGCGCGGTGATTCGCTGCAGCGCGGCGA
CATGGCGGCGCGACTCAGCGGCTCGGGCGATATCTTCGACCGGCGCGGGCGGCGGCCCTGGGCCTCGCTGAATTTCGTGA
CGTCGCATGACGGCTTCACCCTGACGGACGTCGTCAGCTATGCCGAACGCCATAACGAGGCGAACGGCGAAGGCGGACAG
GACGGCCATTCCGATAATTACAGCTCCGGCTGGGGGGTCGAGGGCCCGACCGACGATCCGGCATCCTCGCCGTGCGCGAC
CGGGTGCGTGGCGCCATGCTGGCCACGCTGTTCCTGTCGCAGGGGACGCCGATGCTGCTGGCCGGCGACGAATTCGTGCC
AGACGCAGGACGGCAACAACAATGCCTACTGCCAGGACAACGCGACCGCCTGGCTGGACTGGTCCCTGGCCGACAGCCAG
GCCGGACGCTCGCTGGTCGCGTACGTGGCGCGGCTGGCCGCCCTGCGCCGGGCCTATCCCTCGGTGCGCTGGCGCACCTA
CCTGTATGGCCGTCGCGAAAGCCAGCCCGGCCTGACCGACCTCGCCTGGTACGGCCCCGACGGCGTGCCGATGACGCAGG
ACGCCTGGAACGACGACCATGGCCGCGTCCTGGGCGTGCTGCGGTCCTGCGCCGATACCTGGAACGGCACCGACACCACC
TATCTGCTGATGAACCCGGGGGGCGAGGACGCGGACTGCACCCTGCCGCCGGTCGCGGGCAGCTGGGCCCTGCTGCTGGA
CAGTGCCAATCCCTCGGCCACGACCGGCCCGGGACAGCCCGGCGGGGGGGCGGAAGCCGCGATGCACTGGACGGTTCCCG
CCCATAGCGTCGTCCTGCTGGCCTTCACCCCGCGATCGGACAGATGA

Upstream 100 bases:

>100_bases
TGCCCGAGCCGTCGCATGGCTACGACCAGTCCTGCGTGCTGGTCCTGCCGCCCCTGGCGGTGCTGTACCTCTCGCCCGAA
TCCTTTGGCGGGGGCTGGGC

Downstream 100 bases:

>100_bases
AGGGACCCGCCCATGCGCACGACCCACGCCTTTGCCTGCACGTTCGGCGCCACGATGATCACGCCGCACCAGACGCGTTT
CCGCCTGTGGGCGCCATCGT

Product: glycogen operon protein glgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MMRFPTRLMPGADTPLGASWDGLGVNFAVFSANALRIDLCIFDPSGRREIARFELPERTDEVWHGYLPDARPGLLYGYRA
YGPYEPLRGHRFNPHKLLVDPYARALHGQLSWSDSLFGYRISSPRGDLSIDRRDSAPAMPKSVVTNDAFNWGDDRLPRVP
WDRTVIMEAHLRGLSMRRSELTPVERGTFRALADPQLIDHLLRLGITTLELMPVHAFVKDRFLLERGLTNYWGYNTLAFF
APHAGYLAEGSPHEVRTAIRRLHAAGIEVVLDVVYNHTCEGNELGPTLCYRGLDNAVYYRLVPEDERHLINDTGCGNTLN
LSHPRVLQMVMDSLRHWAVDFHVDGFRFDLCSTLGRESYGFDQACGFFDVLRQDPVLSKLKLIAEPWDPGPGGYQLGNHP
PGFAEWNDRFRDTVRRFWRGDSLQRGDMAARLSGSGDIFDRRGRRPWASLNFVTSHDGFTLTDVVSYAERHNEANGEGGQ
DGHSDNYSSGWGVEGPTDDPASSPCATGCVAPCWPRCSCRRGRRCCWPATNSCQTQDGNNNAYCQDNATAWLDWSLADSQ
AGRSLVAYVARLAALRRAYPSVRWRTYLYGRRESQPGLTDLAWYGPDGVPMTQDAWNDDHGRVLGVLRSCADTWNGTDTT
YLLMNPGGEDADCTLPPVAGSWALLLDSANPSATTGPGQPGGGAEAAMHWTVPAHSVVLLAFTPRSDR

Sequences:

>Translated_708_residues
MMRFPTRLMPGADTPLGASWDGLGVNFAVFSANALRIDLCIFDPSGRREIARFELPERTDEVWHGYLPDARPGLLYGYRA
YGPYEPLRGHRFNPHKLLVDPYARALHGQLSWSDSLFGYRISSPRGDLSIDRRDSAPAMPKSVVTNDAFNWGDDRLPRVP
WDRTVIMEAHLRGLSMRRSELTPVERGTFRALADPQLIDHLLRLGITTLELMPVHAFVKDRFLLERGLTNYWGYNTLAFF
APHAGYLAEGSPHEVRTAIRRLHAAGIEVVLDVVYNHTCEGNELGPTLCYRGLDNAVYYRLVPEDERHLINDTGCGNTLN
LSHPRVLQMVMDSLRHWAVDFHVDGFRFDLCSTLGRESYGFDQACGFFDVLRQDPVLSKLKLIAEPWDPGPGGYQLGNHP
PGFAEWNDRFRDTVRRFWRGDSLQRGDMAARLSGSGDIFDRRGRRPWASLNFVTSHDGFTLTDVVSYAERHNEANGEGGQ
DGHSDNYSSGWGVEGPTDDPASSPCATGCVAPCWPRCSCRRGRRCCWPATNSCQTQDGNNNAYCQDNATAWLDWSLADSQ
AGRSLVAYVARLAALRRAYPSVRWRTYLYGRRESQPGLTDLAWYGPDGVPMTQDAWNDDHGRVLGVLRSCADTWNGTDTT
YLLMNPGGEDADCTLPPVAGSWALLLDSANPSATTGPGQPGGGAEAAMHWTVPAHSVVLLAFTPRSDR
>Mature_708_residues
MMRFPTRLMPGADTPLGASWDGLGVNFAVFSANALRIDLCIFDPSGRREIARFELPERTDEVWHGYLPDARPGLLYGYRA
YGPYEPLRGHRFNPHKLLVDPYARALHGQLSWSDSLFGYRISSPRGDLSIDRRDSAPAMPKSVVTNDAFNWGDDRLPRVP
WDRTVIMEAHLRGLSMRRSELTPVERGTFRALADPQLIDHLLRLGITTLELMPVHAFVKDRFLLERGLTNYWGYNTLAFF
APHAGYLAEGSPHEVRTAIRRLHAAGIEVVLDVVYNHTCEGNELGPTLCYRGLDNAVYYRLVPEDERHLINDTGCGNTLN
LSHPRVLQMVMDSLRHWAVDFHVDGFRFDLCSTLGRESYGFDQACGFFDVLRQDPVLSKLKLIAEPWDPGPGGYQLGNHP
PGFAEWNDRFRDTVRRFWRGDSLQRGDMAARLSGSGDIFDRRGRRPWASLNFVTSHDGFTLTDVVSYAERHNEANGEGGQ
DGHSDNYSSGWGVEGPTDDPASSPCATGCVAPCWPRCSCRRGRRCCWPATNSCQTQDGNNNAYCQDNATAWLDWSLADSQ
AGRSLVAYVARLAALRRAYPSVRWRTYLYGRRESQPGLTDLAWYGPDGVPMTQDAWNDDHGRVLGVLRSCADTWNGTDTT
YLLMNPGGEDADCTLPPVAGSWALLLDSANPSATTGPGQPGGGAEAAMHWTVPAHSVVLLAFTPRSDR

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=659, Percent_Identity=43.8543247344461, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78910; Mature: 78910

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRFPTRLMPGADTPLGASWDGLGVNFAVFSANALRIDLCIFDPSGRREIARFELPERTD
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEEECCCCCCCHHHCCCCCHHH
EVWHGYLPDARPGLLYGYRAYGPYEPLRGHRFNPHKLLVDPYARALHGQLSWSDSLFGYR
HHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCEEE
ISSPRGDLSIDRRDSAPAMPKSVVTNDAFNWGDDRLPRVPWDRTVIMEAHLRGLSMRRSE
ECCCCCCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHH
LTPVERGTFRALADPQLIDHLLRLGITTLELMPVHAFVKDRFLLERGLTNYWGYNTLAFF
CCCCCCCCCHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEE
APHAGYLAEGSPHEVRTAIRRLHAAGIEVVLDVVYNHTCEGNELGPTLCYRGLDNAVYYR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEE
LVPEDERHLINDTGCGNTLNLSHPRVLQMVMDSLRHWAVDFHVDGFRFDLCSTLGRESYG
ECCCCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCC
FDQACGFFDVLRQDPVLSKLKLIAEPWDPGPGGYQLGNHPPGFAEWNDRFRDTVRRFWRG
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
DSLQRGDMAARLSGSGDIFDRRGRRPWASLNFVTSHDGFTLTDVVSYAERHNEANGEGGQ
CCCCCCCEEEEECCCCCHHHCCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCC
DGHSDNYSSGWGVEGPTDDPASSPCATGCVAPCWPRCSCRRGRRCCWPATNSCQTQDGNN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCCCCCCCCCC
NAYCQDNATAWLDWSLADSQAGRSLVAYVARLAALRRAYPSVRWRTYLYGRRESQPGLTD
CEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCE
LAWYGPDGVPMTQDAWNDDHGRVLGVLRSCADTWNGTDTTYLLMNPGGEDADCTLPPVAG
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCC
SWALLLDSANPSATTGPGQPGGGAEAAMHWTVPAHSVVLLAFTPRSDR
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCC
>Mature Secondary Structure
MMRFPTRLMPGADTPLGASWDGLGVNFAVFSANALRIDLCIFDPSGRREIARFELPERTD
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEEECCCCCCCHHHCCCCCHHH
EVWHGYLPDARPGLLYGYRAYGPYEPLRGHRFNPHKLLVDPYARALHGQLSWSDSLFGYR
HHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCEEE
ISSPRGDLSIDRRDSAPAMPKSVVTNDAFNWGDDRLPRVPWDRTVIMEAHLRGLSMRRSE
ECCCCCCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHH
LTPVERGTFRALADPQLIDHLLRLGITTLELMPVHAFVKDRFLLERGLTNYWGYNTLAFF
CCCCCCCCCHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEE
APHAGYLAEGSPHEVRTAIRRLHAAGIEVVLDVVYNHTCEGNELGPTLCYRGLDNAVYYR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEE
LVPEDERHLINDTGCGNTLNLSHPRVLQMVMDSLRHWAVDFHVDGFRFDLCSTLGRESYG
ECCCCCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCC
FDQACGFFDVLRQDPVLSKLKLIAEPWDPGPGGYQLGNHPPGFAEWNDRFRDTVRRFWRG
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
DSLQRGDMAARLSGSGDIFDRRGRRPWASLNFVTSHDGFTLTDVVSYAERHNEANGEGGQ
CCCCCCCEEEEECCCCCHHHCCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCC
DGHSDNYSSGWGVEGPTDDPASSPCATGCVAPCWPRCSCRRGRRCCWPATNSCQTQDGNN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCCCCCCCCCC
NAYCQDNATAWLDWSLADSQAGRSLVAYVARLAALRRAYPSVRWRTYLYGRRESQPGLTD
CEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCE
LAWYGPDGVPMTQDAWNDDHGRVLGVLRSCADTWNGTDTTYLLMNPGGEDADCTLPPVAG
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCC
SWALLLDSANPSATTGPGQPGGGAEAAMHWTVPAHSVVLLAFTPRSDR
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]