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The map label for this gene is poxA [H]

Identifier: 162146334

GI number: 162146334

Start: 506182

End: 507204

Strand: Direct

Name: poxA [H]

Synonym: GDI_0509

Alternate gene names: 162146334

Gene position: 506182-507204 (Clockwise)

Preceding gene: 162146326

Following gene: 162146335

Centisome position: 12.83

GC content: 70.77

Gene sequence:

>1023_bases
ATGACCGACAGCCCCCATCACGACCCCGATCGCATCGCGGGGCGGCTGGCGCTGCTGCGGCGGCGCGCCCTGGTCGGGCG
CGGAATACGGGCCTTCTTCGACGCGCGCGGCTATCTGGAGGTCGAAACCCCCTGCGCCGTCCCCACCCCGGGCGAGGAGG
TGCATCTGCGCGTCTTCAGGACCGAACGCGAACATCATGACGGCACGCGGCAGGCGCTGTTCCTGCATACCAGTCCGGAA
TTCGCGATGAAGCGCATCGTCGCGGCGACCGGCCTGCCGGTCTTCCAACTGGCCCGCGTCTGGCGCAATGCCGAGGGCAG
CCATCTGCATGCCCCGGAATTCACCATGCTGGAATGGTACCGTCCCGGCGCCACCCTGTCCGGCCTGATGGACGAGACCG
AGGCATTGCTGCGCGCCGTCCTGCCGCCGGTGGTCCATCGTCCCGGCGGGCTGGCGGCGGGGGCGGAAATCCGCATCGAC
GCGCCGTTCGAACGCCTGACGATGGAACAGGCCTTCGCCCGCCATGCCGGGCTGGACCTGCTGCCGACCGAAGGCGACGC
CGGGGCGCTGGCGGCGGCGGCGGGGTGCGCCCTGCGTGACGGCGAGGACTGGGAGGACCTGTTCTTCCGCCTGCTGATGG
AACGCGTGGAACCCGCGATCGGCCGCGACCGGCCGACCTTCCTGACCCATTGGCCGGCCAGCCAGGCGGCGCTGGCCCGG
CGCGACCCGGCGGACCCGCGCACCGCACTGCGTTTCGAACTCTATGCCGGCGGGATCGAGCTTGCGAACGCGTTCGAAGA
ATTGACCGATCCCGTGGAACAACGGCGGCGTTTCATGGCCGACCGGGAGCGCCGTGCCGCGTTGTACGGGGCGGCGCCCG
CAACGGCGTGGCCGATGGACGAAGCCCTGCTGGCCGCGCTGGACCACCTGCCGCCCTGCGCCGGGATCGCGCTGGGGTTC
GACCGCCTGGTCATGCTGGCCGCCGGTGCGTTACGGATAGGAGATATCCAGTGGATGGGTTGA

Upstream 100 bases:

>100_bases
GCGGCGCCGGGCGGGGCGGGCGTATCCTCGGGAAGGTTGACCGATCGTGTCATCGGCTGGCTCTACTCGACCGGATTTCG
TCCTGCAACAGGAGTCGCGC

Downstream 100 bases:

>100_bases
AATATTTGTAACCTTTACAGACAGGCCGCCTCAACGTCGGCCGTGTTATCTTAACGTGGCTGTAATTATTTTCCTTCACG
TTCGGTTATCGGTGTCATGA

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTDSPHHDPDRIAGRLALLRRRALVGRGIRAFFDARGYLEVETPCAVPTPGEEVHLRVFRTEREHHDGTRQALFLHTSPE
FAMKRIVAATGLPVFQLARVWRNAEGSHLHAPEFTMLEWYRPGATLSGLMDETEALLRAVLPPVVHRPGGLAAGAEIRID
APFERLTMEQAFARHAGLDLLPTEGDAGALAAAAGCALRDGEDWEDLFFRLLMERVEPAIGRDRPTFLTHWPASQAALAR
RDPADPRTALRFELYAGGIELANAFEELTDPVEQRRRFMADRERRAALYGAAPATAWPMDEALLAALDHLPPCAGIALGF
DRLVMLAAGALRIGDIQWMG

Sequences:

>Translated_340_residues
MTDSPHHDPDRIAGRLALLRRRALVGRGIRAFFDARGYLEVETPCAVPTPGEEVHLRVFRTEREHHDGTRQALFLHTSPE
FAMKRIVAATGLPVFQLARVWRNAEGSHLHAPEFTMLEWYRPGATLSGLMDETEALLRAVLPPVVHRPGGLAAGAEIRID
APFERLTMEQAFARHAGLDLLPTEGDAGALAAAAGCALRDGEDWEDLFFRLLMERVEPAIGRDRPTFLTHWPASQAALAR
RDPADPRTALRFELYAGGIELANAFEELTDPVEQRRRFMADRERRAALYGAAPATAWPMDEALLAALDHLPPCAGIALGF
DRLVMLAAGALRIGDIQWMG
>Mature_339_residues
TDSPHHDPDRIAGRLALLRRRALVGRGIRAFFDARGYLEVETPCAVPTPGEEVHLRVFRTEREHHDGTRQALFLHTSPEF
AMKRIVAATGLPVFQLARVWRNAEGSHLHAPEFTMLEWYRPGATLSGLMDETEALLRAVLPPVVHRPGGLAAGAEIRIDA
PFERLTMEQAFARHAGLDLLPTEGDAGALAAAAGCALRDGEDWEDLFFRLLMERVEPAIGRDRPTFLTHWPASQAALARR
DPADPRTALRFELYAGGIELANAFEELTDPVEQRRRFMADRERRAALYGAAPATAWPMDEALLAALDHLPPCAGIALGFD
RLVMLAAGALRIGDIQWMG

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=333, Percent_Identity=28.8288288288288, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI5031815, Length=333, Percent_Identity=28.8288288288288, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=42.9878048780488, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1789256, Length=325, Percent_Identity=32.9230769230769, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1790571, Length=322, Percent_Identity=33.2298136645963, Blast_Score=151, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17535927, Length=344, Percent_Identity=28.7790697674419, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71994340, Length=344, Percent_Identity=28.7790697674419, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17535925, Length=344, Percent_Identity=28.7790697674419, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24640849, Length=334, Percent_Identity=29.6407185628743, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24640851, Length=334, Percent_Identity=29.6407185628743, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 37580; Mature: 37449

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSPHHDPDRIAGRLALLRRRALVGRGIRAFFDARGYLEVETPCAVPTPGEEVHLRVFR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEE
TEREHHDGTRQALFLHTSPEFAMKRIVAATGLPVFQLARVWRNAEGSHLHAPEFTMLEWY
CCHHHCCCCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHEEE
RPGATLSGLMDETEALLRAVLPPVVHRPGGLAAGAEIRIDAPFERLTMEQAFARHAGLDL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCE
LPTEGDAGALAAAAGCALRDGEDWEDLFFRLLMERVEPAIGRDRPTFLTHWPASQAALAR
ECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
RDPADPRTALRFELYAGGIELANAFEELTDPVEQRRRFMADRERRAALYGAAPATAWPMD
CCCCCCCHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EALLAALDHLPPCAGIALGFDRLVMLAAGALRIGDIQWMG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCEEECC
>Mature Secondary Structure 
TDSPHHDPDRIAGRLALLRRRALVGRGIRAFFDARGYLEVETPCAVPTPGEEVHLRVFR
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEE
TEREHHDGTRQALFLHTSPEFAMKRIVAATGLPVFQLARVWRNAEGSHLHAPEFTMLEWY
CCHHHCCCCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHEEE
RPGATLSGLMDETEALLRAVLPPVVHRPGGLAAGAEIRIDAPFERLTMEQAFARHAGLDL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCE
LPTEGDAGALAAAAGCALRDGEDWEDLFFRLLMERVEPAIGRDRPTFLTHWPASQAALAR
ECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
RDPADPRTALRFELYAGGIELANAFEELTDPVEQRRRFMADRERRAALYGAAPATAWPMD
CCCCCCCHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EALLAALDHLPPCAGIALGFDRLVMLAAGALRIGDIQWMG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA