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The map label for this gene is yjbJ [H]

Identifier: 162146227

GI number: 162146227

Start: 399853

End: 400524

Strand: Direct

Name: yjbJ [H]

Synonym: GDI_0401

Alternate gene names: 162146227

Gene position: 399853-400524 (Clockwise)

Preceding gene: 162146226

Following gene: 162146228

Centisome position: 10.14

GC content: 63.1

Gene sequence:

>672_bases
ATGAGGCGCCGGTTTTTCATGTCCGTCGGCGTGCTGGCCATGCTCGCGCTGCTGCTCTCGCCCGTCCCGGTCCAGGCGCA
GGATTGGGACAATCTCGTGCGTCAGGCAGCGAAGCAAAATGCGATCCCGGCGACGTGGGTGCGGGCGGTTCTGCGGGCTG
AAAGCGCTGGCGATCCGCACGCGGTTTCCGGTGCCGGTGCGATGGGTCTGATGCAGCTCATGCCGGGCACGTGGAAGGAT
GTCCGGCGCACGCTCAATCTCGGTGCCGATCCCTTCGATCCGCATGACAATATCGCGGCAGGGGCAGCCTATCTGCGATG
GCTGCACGACCGTTATGGCGATGCGGGTTTTCTCGCCGCCTACAATGCCGGACCCGCCCGTTACGACGACCATCTGGCGA
CTGGCCGACCATTGCCTGGCGAGACGGTGTCCTATGTCGCGTCTGTCGCCCGGCTGATCGATGACCCGTCGTTCAGCGTG
CATTTTTCCATCACTCCAGCGCGTGACTGGGCCGCCGCCACACTCTTCATTTCCGGCAGCGAAAGTGCGTTTCCATCGTC
ATCCCGAAGCATTTTTTTAAGCCGATCCGGGCAGGGGATTTTCACTCCGTCCGTGCTCTTTGCAGGGCATGTCCGTAGCG
TGCCGGGAGGGGGTTCGACGCAGGCGAAGTGA

Upstream 100 bases:

>100_bases
TCATGCAACCGGTCCGGGCCACGCTGTTCCAGACGGGACGCGGTGGACGCGAGTGGCAACTGGTCTGGAACCGGTCCGTA
AAACGTCCGGGAGGTCGGGA

Downstream 100 bases:

>100_bases
ATCACGAAAACCCCGGAAAAGGACAGAAAAAGTTAGTGCCAGCCGTAATCGGGCCTGAGCGGTCGTCGTCGATTGGCGGT
CTCCCGGGCCGGAAAGAAGG

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRRRFFMSVGVLAMLALLLSPVPVQAQDWDNLVRQAAKQNAIPATWVRAVLRAESAGDPHAVSGAGAMGLMQLMPGTWKD
VRRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAAYNAGPARYDDHLATGRPLPGETVSYVASVARLIDDPSFSV
HFSITPARDWAAATLFISGSESAFPSSSRSIFLSRSGQGIFTPSVLFAGHVRSVPGGGSTQAK

Sequences:

>Translated_223_residues
MRRRFFMSVGVLAMLALLLSPVPVQAQDWDNLVRQAAKQNAIPATWVRAVLRAESAGDPHAVSGAGAMGLMQLMPGTWKD
VRRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAAYNAGPARYDDHLATGRPLPGETVSYVASVARLIDDPSFSV
HFSITPARDWAAATLFISGSESAFPSSSRSIFLSRSGQGIFTPSVLFAGHVRSVPGGGSTQAK
>Mature_223_residues
MRRRFFMSVGVLAMLALLLSPVPVQAQDWDNLVRQAAKQNAIPATWVRAVLRAESAGDPHAVSGAGAMGLMQLMPGTWKD
VRRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAAYNAGPARYDDHLATGRPLPGETVSYVASVARLIDDPSFSV
HFSITPARDWAAATLFISGSESAFPSSSRSIFLSRSGQGIFTPSVLFAGHVRSVPGGGSTQAK

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=109, Percent_Identity=34.8623853211009, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23823; Mature: 23823

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRFFMSVGVLAMLALLLSPVPVQAQDWDNLVRQAAKQNAIPATWVRAVLRAESAGDPH
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
AVSGAGAMGLMQLMPGTWKDVRRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAA
CCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
YNAGPARYDDHLATGRPLPGETVSYVASVARLIDDPSFSVHFSITPARDWAAATLFISGS
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEECC
ESAFPSSSRSIFLSRSGQGIFTPSVLFAGHVRSVPGGGSTQAK
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRRRFFMSVGVLAMLALLLSPVPVQAQDWDNLVRQAAKQNAIPATWVRAVLRAESAGDPH
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
AVSGAGAMGLMQLMPGTWKDVRRTLNLGADPFDPHDNIAAGAAYLRWLHDRYGDAGFLAA
CCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEE
YNAGPARYDDHLATGRPLPGETVSYVASVARLIDDPSFSVHFSITPARDWAAATLFISGS
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEECC
ESAFPSSSRSIFLSRSGQGIFTPSVLFAGHVRSVPGGGSTQAK
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]