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The map label for this gene is afr [H]

Identifier: 162146181

GI number: 162146181

Start: 365482

End: 366624

Strand: Direct

Name: afr [H]

Synonym: GDI_0353

Alternate gene names: 162146181

Gene position: 365482-366624 (Clockwise)

Preceding gene: 162146180

Following gene: 162146184

Centisome position: 9.27

GC content: 67.19

Gene sequence:

>1143_bases
ATGACGAAAAACGTTTCAAGACGCACGCTGGGCATAGGGCTGATCGGCAGCGGTTTCATGGGTAAGGCCCATGCGCTGGC
CTTTCGCACGGTGGGCGGGGTCTTCCCCCTGGCCGCCGAGCCCAGGCTGGAAATCCTGGCGGACATCTCCGCCGACAAGG
CGGCGCGCGCGGCCTATGAACTCGGCTTCGCGCGCTTCACCGCGCAATGGGAACGGCTTGTCCTGGACCCGGCGGTCGAT
ATCGTCGCCATCACCACGCCCAACCGCCAGCACGCCCCGATGGCCCTGGCCGCGATCGCGGCGGGCAAGCATGTCTATTG
CGAAAAACCGCTGGCCACGTCACTGGAAGACGCCCGCGCCATGACCGAGGCCGCCGAGCGCGCCGGGGTGGTGACGCAGG
TCGGCTTCAACTACCTGCGCAACCCGATGGTCCACACGGCGAAGGAAATCATCGCCAGTGGCGAGATCGGGGACATCATC
GGGTTTTCCGGCCAGTATATCGCCGGCTTCATGGGCACGCCCGAAGGGTCCTGGTCCTGGCGTTTCCAGCCCGAGGAAGC
GGGCGGCGCACTGGCCGATATCGGCTCGCACCTGATTTCCATGAGCCGCTACCTGCTGGGCGAGGTCGCGTCGGTCTGCG
CCGGCCTGGATACCGTCTATCCCACGCGCACGTCGTCCGACGGCCAGTCGCACCGGGTCGGCATCGACGACCAGGCCGCG
ATCCTGGCCCGCTTCCAGGACCACCCGTTCACCGCCACGCTGTTCACGTCCTGGGTCGCGACCGGGCGTGAAATGGGCCT
GTCGTTCGACGTCACCGGGTCGAAGGGGGCGTTGCGCTTCACGCAGGAACGCCTGAACGAACTGGAACTGTTCAAATCCG
GGCAGGCCGCCAACCGCCGTGGCTTCACGCGCCTGACCGCCTCGCCCGCCTATCCGCCCTACGGGGCCTTTACCCCCGGC
GCCGGCCATCAGCTTGGCTTCAACGACCTGAAGGTCATCGAGGTCCGGAACCTGATCGACGCCGTCTGCGGCGTGGCGCC
CGCCGAGACACCCTTCCGCGATGCGCTGCGCACCGCCATGGTCATGGAAGCCGTCCGGGATTCGTCCCGGACCCGGCAGT
GGATGGACGTTCCCGCCCAATAA

Upstream 100 bases:

>100_bases
TCGACCAGTCGGTCTGGGGCGCGTACGATGATTCGCCCCATCCCGCCCAGGCCATGCGCCTGCACGGCGAATAACGAAAA
AAAGAAACCAGGAGCAGGTC

Downstream 100 bases:

>100_bases
GGTGGGAAAATCACGTGATTCGGAAAAATGGAATATATTACATAAAAAATCCGGCGTGCCGATGACGGCACGCCGGATGA
ATTTCGCGGCCTGGTTCTAC

Product: oxidoreductase protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTKNVSRRTLGIGLIGSGFMGKAHALAFRTVGGVFPLAAEPRLEILADISADKAARAAYELGFARFTAQWERLVLDPAVD
IVAITTPNRQHAPMALAAIAAGKHVYCEKPLATSLEDARAMTEAAERAGVVTQVGFNYLRNPMVHTAKEIIASGEIGDII
GFSGQYIAGFMGTPEGSWSWRFQPEEAGGALADIGSHLISMSRYLLGEVASVCAGLDTVYPTRTSSDGQSHRVGIDDQAA
ILARFQDHPFTATLFTSWVATGREMGLSFDVTGSKGALRFTQERLNELELFKSGQAANRRGFTRLTASPAYPPYGAFTPG
AGHQLGFNDLKVIEVRNLIDAVCGVAPAETPFRDALRTAMVMEAVRDSSRTRQWMDVPAQ

Sequences:

>Translated_380_residues
MTKNVSRRTLGIGLIGSGFMGKAHALAFRTVGGVFPLAAEPRLEILADISADKAARAAYELGFARFTAQWERLVLDPAVD
IVAITTPNRQHAPMALAAIAAGKHVYCEKPLATSLEDARAMTEAAERAGVVTQVGFNYLRNPMVHTAKEIIASGEIGDII
GFSGQYIAGFMGTPEGSWSWRFQPEEAGGALADIGSHLISMSRYLLGEVASVCAGLDTVYPTRTSSDGQSHRVGIDDQAA
ILARFQDHPFTATLFTSWVATGREMGLSFDVTGSKGALRFTQERLNELELFKSGQAANRRGFTRLTASPAYPPYGAFTPG
AGHQLGFNDLKVIEVRNLIDAVCGVAPAETPFRDALRTAMVMEAVRDSSRTRQWMDVPAQ
>Mature_379_residues
TKNVSRRTLGIGLIGSGFMGKAHALAFRTVGGVFPLAAEPRLEILADISADKAARAAYELGFARFTAQWERLVLDPAVDI
VAITTPNRQHAPMALAAIAAGKHVYCEKPLATSLEDARAMTEAAERAGVVTQVGFNYLRNPMVHTAKEIIASGEIGDIIG
FSGQYIAGFMGTPEGSWSWRFQPEEAGGALADIGSHLISMSRYLLGEVASVCAGLDTVYPTRTSSDGQSHRVGIDDQAAI
LARFQDHPFTATLFTSWVATGREMGLSFDVTGSKGALRFTQERLNELELFKSGQAANRRGFTRLTASPAYPPYGAFTPGA
GHQLGFNDLKVIEVRNLIDAVCGVAPAETPFRDALRTAMVMEAVRDSSRTRQWMDVPAQ

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=287, Percent_Identity=24.390243902439, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI87081947, Length=217, Percent_Identity=29.4930875576037, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787574, Length=209, Percent_Identity=28.2296650717703, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082405, Length=139, Percent_Identity=34.5323741007194, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323975, Length=211, Percent_Identity=27.4881516587678, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24581117, Length=231, Percent_Identity=26.4069264069264, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 40898; Mature: 40767

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNVSRRTLGIGLIGSGFMGKAHALAFRTVGGVFPLAAEPRLEILADISADKAARAAYE
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHEECCCHHHHHHHHHH
LGFARFTAQWERLVLDPAVDIVAITTPNRQHAPMALAAIAAGKHVYCEKPLATSLEDARA
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHH
MTEAAERAGVVTQVGFNYLRNPMVHTAKEIIASGEIGDIIGFSGQYIAGFMGTPEGSWSW
HHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCEE
RFQPEEAGGALADIGSHLISMSRYLLGEVASVCAGLDTVYPTRTSSDGQSHRVGIDDQAA
EECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHH
ILARFQDHPFTATLFTSWVATGREMGLSFDVTGSKGALRFTQERLNELELFKSGQAANRR
HHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GFTRLTASPAYPPYGAFTPGAGHQLGFNDLKVIEVRNLIDAVCGVAPAETPFRDALRTAM
CCEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VMEAVRDSSRTRQWMDVPAQ
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKNVSRRTLGIGLIGSGFMGKAHALAFRTVGGVFPLAAEPRLEILADISADKAARAAYE
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHEECCCHHHHHHHHHH
LGFARFTAQWERLVLDPAVDIVAITTPNRQHAPMALAAIAAGKHVYCEKPLATSLEDARA
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCEEECCCHHHHHHHHHH
MTEAAERAGVVTQVGFNYLRNPMVHTAKEIIASGEIGDIIGFSGQYIAGFMGTPEGSWSW
HHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCCCCCEE
RFQPEEAGGALADIGSHLISMSRYLLGEVASVCAGLDTVYPTRTSSDGQSHRVGIDDQAA
EECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHH
ILARFQDHPFTATLFTSWVATGREMGLSFDVTGSKGALRFTQERLNELELFKSGQAANRR
HHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GFTRLTASPAYPPYGAFTPGAGHQLGFNDLKVIEVRNLIDAVCGVAPAETPFRDALRTAM
CCEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VMEAVRDSSRTRQWMDVPAQ
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA