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The map label for this gene is pntA [H]

Identifier: 162146169

GI number: 162146169

Start: 353667

End: 354842

Strand: Direct

Name: pntA [H]

Synonym: GDI_0341

Alternate gene names: 162146169

Gene position: 353667-354842 (Clockwise)

Preceding gene: 162146168

Following gene: 162146170

Centisome position: 8.97

GC content: 68.2

Gene sequence:

>1176_bases
ATGAGTCTTAAGATTGCAGCGATCAGGGAAAAGGGGGCGGACGAACGGCGCGTCGCCCTGGTCCCGGACGTCGCGGCCCG
TATCGGGCGGCTGGGATGCGATGTGGCGCTGGAAGCGGGCGCGGGCGTCCCGTCATCCTATCCCGACGCCAGCTACAAGG
ACGTGCGGATCGAGCCCGACCGGAAGGCGCTGCTCGGTTCGGCGGATGTCGTTCTGGCCATCCAGCCGCCGGCAACCGGG
GACGTGCAGGCCATGAAGCCCGGCGCGATCCTGATTTCCTTCGTATATGGCAGGAACAATCCAGATCTGGTCAAAGCCCT
GCGCGACGGGCGGATCACGGCCTTCGCCATGGAGCAGGTGCCGCGCATCAGCCGCGCGCAGGCCATGGATGCCCTGTCCA
GCCAGGCGACGCTGGCGGGGTATTATTCCGTCCTGCTGGGCGCCGTGCAGATGCCGAAAATCCTGCCGATGATGACGACG
GCGGTGGGCTCGCTCCGCGCGGCTCAGGTGCTGGTCATGGGCCTGGGCGTGGCCGGCCTGCAGGCGCTGGCGACGGCACG
CCGCCTGGGCGCGGTGACCGAAGGCTACGACGTGCGGCCCGAGACCCGGGACGAGGCTCGCTCGCTGGGCGCGAAATTCG
TCGAAACCGGCGTGGATGCGACGGGTCAGGGCGGCTATGCGCGCGAACTCACCGCCGAGGAGAAGGCGACGGTGCGCGCC
GTTCTCAGCGACCACATCGCGCGATCCGACCTGGTCATCACGACGGCCGCCGTGCCGGGCCGCCAGGCCCCGCGCCTGAT
CGACGCCCGGCAGATCGCCAGGATGAAGCCCGGATCGGTGATCGTCGACCTGGGTGCCGAGGGCGGGGGCAATTGCGAGG
GCACCCGGGCCGGCGAAACCGTGCAGGCCGGTCCGGCGACCCTGATCGGTCCTGTCAACCTGCCGTCGCGCTTGGCCGAA
CAGGCAAGCGAACTGTATGCGAAGAACGTCCTCAACCTGCTGGAACAGATCGTCAGGGACGGCAAGCTGTCGCTGGACAT
GACGGACGAGGTCATCGCGAAGACGCTCGTGACCCATGACGGCAGGATCGTCAACGATGCGGTGCGGCAGGTCGTGGAAG
GCCCGCCTTCCGTGAAGCCGGCCCTCGCGGTGAAAGAAGACGCGCCATGCTCATGA

Upstream 100 bases:

>100_bases
AGTCTCTCATATTACCTTTTCACGAAAAATAAATAGTAATGCAAGTATATCTGCGACGAGAGAGCAGAATTCAGCATGCA
ATACAATGTGAGAACTGGTC

Downstream 100 bases:

>100_bases
CCTCGATGACGTTCATCATCGCGCTCTACATCTTCGTGCTGGCGGCCTTCACCGGCTATGTCGTCATCAGCCGCGTGCCG
TCGATCCTGCATACCCCGCT

Product: NAD(P) transhydrogenase subunit alpha part 1

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Pyridine nucleotide transhydrogenase subunit alpha 1 [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSLKIAAIREKGADERRVALVPDVAARIGRLGCDVALEAGAGVPSSYPDASYKDVRIEPDRKALLGSADVVLAIQPPATG
DVQAMKPGAILISFVYGRNNPDLVKALRDGRITAFAMEQVPRISRAQAMDALSSQATLAGYYSVLLGAVQMPKILPMMTT
AVGSLRAAQVLVMGLGVAGLQALATARRLGAVTEGYDVRPETRDEARSLGAKFVETGVDATGQGGYARELTAEEKATVRA
VLSDHIARSDLVITTAAVPGRQAPRLIDARQIARMKPGSVIVDLGAEGGGNCEGTRAGETVQAGPATLIGPVNLPSRLAE
QASELYAKNVLNLLEQIVRDGKLSLDMTDEVIAKTLVTHDGRIVNDAVRQVVEGPPSVKPALAVKEDAPCS

Sequences:

>Translated_391_residues
MSLKIAAIREKGADERRVALVPDVAARIGRLGCDVALEAGAGVPSSYPDASYKDVRIEPDRKALLGSADVVLAIQPPATG
DVQAMKPGAILISFVYGRNNPDLVKALRDGRITAFAMEQVPRISRAQAMDALSSQATLAGYYSVLLGAVQMPKILPMMTT
AVGSLRAAQVLVMGLGVAGLQALATARRLGAVTEGYDVRPETRDEARSLGAKFVETGVDATGQGGYARELTAEEKATVRA
VLSDHIARSDLVITTAAVPGRQAPRLIDARQIARMKPGSVIVDLGAEGGGNCEGTRAGETVQAGPATLIGPVNLPSRLAE
QASELYAKNVLNLLEQIVRDGKLSLDMTDEVIAKTLVTHDGRIVNDAVRQVVEGPPSVKPALAVKEDAPCS
>Mature_390_residues
SLKIAAIREKGADERRVALVPDVAARIGRLGCDVALEAGAGVPSSYPDASYKDVRIEPDRKALLGSADVVLAIQPPATGD
VQAMKPGAILISFVYGRNNPDLVKALRDGRITAFAMEQVPRISRAQAMDALSSQATLAGYYSVLLGAVQMPKILPMMTTA
VGSLRAAQVLVMGLGVAGLQALATARRLGAVTEGYDVRPETRDEARSLGAKFVETGVDATGQGGYARELTAEEKATVRAV
LSDHIARSDLVITTAAVPGRQAPRLIDARQIARMKPGSVIVDLGAEGGGNCEGTRAGETVQAGPATLIGPVNLPSRLAEQ
ASELYAKNVLNLLEQIVRDGKLSLDMTDEVIAKTLVTHDGRIVNDAVRQVVEGPPSVKPALAVKEDAPCS

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=381, Percent_Identity=35.6955380577428, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI122939153, Length=381, Percent_Identity=35.6955380577428, Blast_Score=225, Evalue=5e-59,
Organism=Escherichia coli, GI1787887, Length=370, Percent_Identity=37.2972972972973, Blast_Score=242, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17550456, Length=363, Percent_Identity=39.9449035812672, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 40956; Mature: 40824

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKIAAIREKGADERRVALVPDVAARIGRLGCDVALEAGAGVPSSYPDASYKDVRIEPD
CCEEEEEHHHCCCCCCEEEECCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEECCC
RKALLGSADVVLAIQPPATGDVQAMKPGAILISFVYGRNNPDLVKALRDGRITAFAMEQV
HHHHCCCCCEEEEECCCCCCCCEECCCCEEEEEEEECCCCHHHHHHHHCCCEEEEHHHHC
PRISRAQAMDALSSQATLAGYYSVLLGAVQMPKILPMMTTAVGSLRAAQVLVMGLGVAGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QALATARRLGAVTEGYDVRPETRDEARSLGAKFVETGVDATGQGGYARELTAEEKATVRA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHH
VLSDHIARSDLVITTAAVPGRQAPRLIDARQIARMKPGSVIVDLGAEGGGNCEGTRAGET
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCE
VQAGPATLIGPVNLPSRLAEQASELYAKNVLNLLEQIVRDGKLSLDMTDEVIAKTLVTHD
EECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCC
GRIVNDAVRQVVEGPPSVKPALAVKEDAPCS
CCHHHHHHHHHHCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SLKIAAIREKGADERRVALVPDVAARIGRLGCDVALEAGAGVPSSYPDASYKDVRIEPD
CEEEEEHHHCCCCCCEEEECCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEECCC
RKALLGSADVVLAIQPPATGDVQAMKPGAILISFVYGRNNPDLVKALRDGRITAFAMEQV
HHHHCCCCCEEEEECCCCCCCCEECCCCEEEEEEEECCCCHHHHHHHHCCCEEEEHHHHC
PRISRAQAMDALSSQATLAGYYSVLLGAVQMPKILPMMTTAVGSLRAAQVLVMGLGVAGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QALATARRLGAVTEGYDVRPETRDEARSLGAKFVETGVDATGQGGYARELTAEEKATVRA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHH
VLSDHIARSDLVITTAAVPGRQAPRLIDARQIARMKPGSVIVDLGAEGGGNCEGTRAGET
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCE
VQAGPATLIGPVNLPSRLAEQASELYAKNVLNLLEQIVRDGKLSLDMTDEVIAKTLVTHD
EECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHCC
GRIVNDAVRQVVEGPPSVKPALAVKEDAPCS
CCHHHHHHHHHHCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9823893; 9335295; 8662004 [H]