Click here to switch to the map view.

The map label for this gene is dosP [H]

Identifier: 162146128

GI number: 162146128

Start: 309823

End: 311535

Strand: Direct

Name: dosP [H]

Synonym: GDI_0299

Alternate gene names: 162146128

Gene position: 309823-311535 (Clockwise)

Preceding gene: 162146127

Following gene: 162146129

Centisome position: 7.86

GC content: 61.47

Gene sequence:

>1713_bases
TTGTGTGCCGAGGTCGAACGTCTTGTGCCGGACAGCGTGGCGACAGTACTGCGGGTCAATTTGGAGCAGGGGCTCGAAAT
TCTCTCGGGCGACAGTCTTCCGCGCGCGAAAATCGCTCTGCTGGAACACATGACCCTGACGGATGCGGATATTGCCGCTC
TTCGCGCGAACCCCGAGGAAGCATACAGAATCGTATGGCGACCGGGAGAGGACGAAACGCACGACATCGCATTGTCCGAT
TGCCGGGCGTCGGCCCTCTTCGACGGCAGGGGCCAGCTGATCGGCATCTTCGCCCTGTATTCGCGGCATCGGCACGGCAT
CGCGGACTGGGGGGAAAAAATCGTCTCGCACTGCCGTTCCTCGTGCGTTGCGATCATCGAATACGGCAGAACCCGCAGGC
GGCTGGACCAAATCGACCGCCACGACGCGCTGACCGGCCTTCCGAACCGCGCCGCGATATTCGATCTTTTGCAAAAGATG
ACGACGGAAACGCCAACGTCACCATTCGCGCTCATCATCCTGGACGTGGACCTGTTTCAGGACTTCAACGATGCGATGGG
GTACGAACAGGGTGACCGTCTGCTGCAGATCGTGGCCTGCCGATTTTCCACCCATTGCCGCGGCGATGCCGTCATCGGCC
GTCTCGGCGGAGACAACTTCGCCATCGTCATTCCCGGAGAACAGGACACGGCGGCAAACGACCTCGCCCGGGATCTCGTC
GCAACCCTCCGCAGCCCCATCCGCATCCGCGGGCAGGACATCGTCATATCGTGCAGTACAGGCATCGGCACGTTTCCGGA
GAACGGGACCGAGGTGGAACAGGTCGTCCATCACGCGCAGATCGCGCTGCGCGAGGCGAAGAAGGCCACACGCGGCAGCG
TGCAGAACGCCCCCGCCGCCACCGGAGATGACGTCGAGGCCCGGGTCCTTCTCGGCTCGGCCATGCGCAAGGCCGTCCAG
AAATCCGAATTCCGCCTGTTCTACCAACCGCAGATCTGCATGGAAAGCGGAACGATATATGGGGTCGAGGCTCTGGCCCG
CTGGTATCATCCTGCAATGGGGCTCATTCAGCCGTCGCAATTCATCCCGGTCGCCGAACAGACGGGGCAGATCGAAATCA
TCGACAGATGGTCCATGGAAGAAGCGTGTCGGCAGATGGCGGAATGGGACCGGGCCGGAATGCGGTTGCCCACGGTCAGC
GTGAACGTCTCTGCCGTCAGTATCCGAAACACCGATCTGCCCCGGCGGATCGCCGCCCTTCTCAAGCGGTATCGGCTGGA
ACCGCGCCGCCTGACGGTCGAAATTACGGAAAGCGCCATGATGCAGCAGAGCCGCGACACCGGGGCGACCCTTTCCGCCA
TGCTTGCGATCGGAATAGGCCTGGCGCTCGATGATTTCGGAACCGGCTTTTCCTCCCTGTCGCGCCTGACCCATCTTCCC
CTGACGGAAATCAAGATCGACCGCAGCTTCGTCACAAGTCTGGAGCGGGACGTCGGTTCGATGATCGTCACCGAGGCGGC
GATCACGATCGGAAAGCGACTGGGGGTGAAAGTGGTCACCGAAGGCGTCGAAGAGATCGCGCAGGTAAGGAAACTGCGTC
AACTGGGCTGCGATGTCGTGCAGGGCTACCTGATCAGCAAGCCGCTGCCGTCAGCCGAAATTCCGAACTGGCTGCAATCT
CGTGACGGAACGATCACCGAAAGTGACGAATAG

Upstream 100 bases:

>100_bases
TTTGGAAAGAATCCTGGATCTTCATAATCGTATCCTAAAACAGTTCTTATTCGAAACGACATGGCGGGGTACAGGGCAGG
GTCAGGATATCGCCGGCGTA

Downstream 100 bases:

>100_bases
GTCCTTGTAAGTGCAGAACAGGTTTTGTTCATATGAATTTCGTAACAAAAATGGATATTAACAGAATTAAATTCCCATGA
AGCGACGATAGTCGTCGTCA

Product: GGDEF family signaling protein

Products: NA

Alternate protein names: Direct oxygen sensing phosphodiesterase; Direct oxygen sensor protein; Ec DOS; Heme-regulated cyclic di-GMP phosphodiesterase, [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MCAEVERLVPDSVATVLRVNLEQGLEILSGDSLPRAKIALLEHMTLTDADIAALRANPEEAYRIVWRPGEDETHDIALSD
CRASALFDGRGQLIGIFALYSRHRHGIADWGEKIVSHCRSSCVAIIEYGRTRRRLDQIDRHDALTGLPNRAAIFDLLQKM
TTETPTSPFALIILDVDLFQDFNDAMGYEQGDRLLQIVACRFSTHCRGDAVIGRLGGDNFAIVIPGEQDTAANDLARDLV
ATLRSPIRIRGQDIVISCSTGIGTFPENGTEVEQVVHHAQIALREAKKATRGSVQNAPAATGDDVEARVLLGSAMRKAVQ
KSEFRLFYQPQICMESGTIYGVEALARWYHPAMGLIQPSQFIPVAEQTGQIEIIDRWSMEEACRQMAEWDRAGMRLPTVS
VNVSAVSIRNTDLPRRIAALLKRYRLEPRRLTVEITESAMMQQSRDTGATLSAMLAIGIGLALDDFGTGFSSLSRLTHLP
LTEIKIDRSFVTSLERDVGSMIVTEAAITIGKRLGVKVVTEGVEEIAQVRKLRQLGCDVVQGYLISKPLPSAEIPNWLQS
RDGTITESDE

Sequences:

>Translated_570_residues
MCAEVERLVPDSVATVLRVNLEQGLEILSGDSLPRAKIALLEHMTLTDADIAALRANPEEAYRIVWRPGEDETHDIALSD
CRASALFDGRGQLIGIFALYSRHRHGIADWGEKIVSHCRSSCVAIIEYGRTRRRLDQIDRHDALTGLPNRAAIFDLLQKM
TTETPTSPFALIILDVDLFQDFNDAMGYEQGDRLLQIVACRFSTHCRGDAVIGRLGGDNFAIVIPGEQDTAANDLARDLV
ATLRSPIRIRGQDIVISCSTGIGTFPENGTEVEQVVHHAQIALREAKKATRGSVQNAPAATGDDVEARVLLGSAMRKAVQ
KSEFRLFYQPQICMESGTIYGVEALARWYHPAMGLIQPSQFIPVAEQTGQIEIIDRWSMEEACRQMAEWDRAGMRLPTVS
VNVSAVSIRNTDLPRRIAALLKRYRLEPRRLTVEITESAMMQQSRDTGATLSAMLAIGIGLALDDFGTGFSSLSRLTHLP
LTEIKIDRSFVTSLERDVGSMIVTEAAITIGKRLGVKVVTEGVEEIAQVRKLRQLGCDVVQGYLISKPLPSAEIPNWLQS
RDGTITESDE
>Mature_570_residues
MCAEVERLVPDSVATVLRVNLEQGLEILSGDSLPRAKIALLEHMTLTDADIAALRANPEEAYRIVWRPGEDETHDIALSD
CRASALFDGRGQLIGIFALYSRHRHGIADWGEKIVSHCRSSCVAIIEYGRTRRRLDQIDRHDALTGLPNRAAIFDLLQKM
TTETPTSPFALIILDVDLFQDFNDAMGYEQGDRLLQIVACRFSTHCRGDAVIGRLGGDNFAIVIPGEQDTAANDLARDLV
ATLRSPIRIRGQDIVISCSTGIGTFPENGTEVEQVVHHAQIALREAKKATRGSVQNAPAATGDDVEARVLLGSAMRKAVQ
KSEFRLFYQPQICMESGTIYGVEALARWYHPAMGLIQPSQFIPVAEQTGQIEIIDRWSMEEACRQMAEWDRAGMRLPTVS
VNVSAVSIRNTDLPRRIAALLKRYRLEPRRLTVEITESAMMQQSRDTGATLSAMLAIGIGLALDDFGTGFSSLSRLTHLP
LTEIKIDRSFVTSLERDVGSMIVTEAAITIGKRLGVKVVTEGVEEIAQVRKLRQLGCDVVQGYLISKPLPSAEIPNWLQS
RDGTITESDE

Specific function: In association with DosC is involved in the production and removal of the second messenger c-di-GMP in response to changing O(2) levels. Has phosphodiesterase (PDE) activity with c- di-GMP (PubMed:15995192), very poor activity on cAMP (PubMed:15995192) bu

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081921, Length=449, Percent_Identity=36.5256124721604, Blast_Score=311, Evalue=1e-85,
Organism=Escherichia coli, GI1787541, Length=497, Percent_Identity=30.3822937625755, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI226510982, Length=411, Percent_Identity=28.7104622871046, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1790496, Length=250, Percent_Identity=32.4, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI87081743, Length=243, Percent_Identity=32.9218106995885, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1786507, Length=269, Percent_Identity=29.368029739777, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788502, Length=252, Percent_Identity=31.3492063492063, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081980, Length=240, Percent_Identity=35, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI87081845, Length=250, Percent_Identity=32, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788849, Length=288, Percent_Identity=30.2083333333333, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1787055, Length=253, Percent_Identity=28.4584980237154, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI87082096, Length=283, Percent_Identity=28.2685512367491, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87082007, Length=159, Percent_Identity=33.9622641509434, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1788381, Length=211, Percent_Identity=27.9620853080569, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI87081977, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI145693134, Length=202, Percent_Identity=29.2079207920792, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786584, Length=176, Percent_Identity=27.8409090909091, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787262, Length=200, Percent_Identity=25.5, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR012226
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF00989 PAS; PF08448 PAS_4 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 62871; Mature: 62871

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAEVERLVPDSVATVLRVNLEQGLEILSGDSLPRAKIALLEHMTLTDADIAALRANPEE
CCCCHHHHCCHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHH
AYRIVWRPGEDETHDIALSDCRASALFDGRGQLIGIFALYSRHRHGIADWGEKIVSHCRS
EEEEEECCCCCCCCCEEHHHHHHHHEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHH
SCVAIIEYGRTRRRLDQIDRHDALTGLPNRAAIFDLLQKMTTETPTSPFALIILDVDLFQ
HHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHH
DFNDAMGYEQGDRLLQIVACRFSTHCRGDAVIGRLGGDNFAIVIPGEQDTAANDLARDLV
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHH
ATLRSPIRIRGQDIVISCSTGIGTFPENGTEVEQVVHHAQIALREAKKATRGSVQNAPAA
HHHCCCCEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TGDDVEARVLLGSAMRKAVQKSEFRLFYQPQICMESGTIYGVEALARWYHPAMGLIQPSQ
CCCCCHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCEEHHHHHHHHHHHHCCCCCCCC
FIPVAEQTGQIEIIDRWSMEEACRQMAEWDRAGMRLPTVSVNVSAVSIRNTDLPRRIAAL
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCHHHHHHH
LKRYRLEPRRLTVEITESAMMQQSRDTGATLSAMLAIGIGLALDDFGTGFSSLSRLTHLP
HHHHCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
LTEIKIDRSFVTSLERDVGSMIVTEAAITIGKRLGVKVVTEGVEEIAQVRKLRQLGCDVV
HHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHH
QGYLISKPLPSAEIPNWLQSRDGTITESDE
HHHHHCCCCCCCCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MCAEVERLVPDSVATVLRVNLEQGLEILSGDSLPRAKIALLEHMTLTDADIAALRANPEE
CCCCHHHHCCHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHH
AYRIVWRPGEDETHDIALSDCRASALFDGRGQLIGIFALYSRHRHGIADWGEKIVSHCRS
EEEEEECCCCCCCCCEEHHHHHHHHEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHH
SCVAIIEYGRTRRRLDQIDRHDALTGLPNRAAIFDLLQKMTTETPTSPFALIILDVDLFQ
HHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHH
DFNDAMGYEQGDRLLQIVACRFSTHCRGDAVIGRLGGDNFAIVIPGEQDTAANDLARDLV
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHH
ATLRSPIRIRGQDIVISCSTGIGTFPENGTEVEQVVHHAQIALREAKKATRGSVQNAPAA
HHHCCCCEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TGDDVEARVLLGSAMRKAVQKSEFRLFYQPQICMESGTIYGVEALARWYHPAMGLIQPSQ
CCCCCHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCEEHHHHHHHHHHHHCCCCCCCC
FIPVAEQTGQIEIIDRWSMEEACRQMAEWDRAGMRLPTVSVNVSAVSIRNTDLPRRIAAL
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCHHHHHHH
LKRYRLEPRRLTVEITESAMMQQSRDTGATLSAMLAIGIGLALDDFGTGFSSLSRLTHLP
HHHHCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
LTEIKIDRSFVTSLERDVGSMIVTEAAITIGKRLGVKVVTEGVEEIAQVRKLRQLGCDVV
HHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHH
QGYLISKPLPSAEIPNWLQSRDGTITESDE
HHHHHCCCCCCCCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]