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The map label for this gene is ycgF [H]

Identifier: 162146109

GI number: 162146109

Start: 288176

End: 289015

Strand: Reverse

Name: ycgF [H]

Synonym: GDI_0278

Alternate gene names: 162146109

Gene position: 289015-288176 (Counterclockwise)

Preceding gene: 162146110

Following gene: 162146108

Centisome position: 7.33

GC content: 64.29

Gene sequence:

>840_bases
ATGGGCTGCTTTCCGGGACCGACATGCGATAGTCATGAATTCGAATATCCACGTCCGGAACCCCCCATGACAGCATCACC
CCCGTCCAGTCGCGCCCCCTGCCAGGCCTGCCACGACCATCAGGATCTGTTTCCGTTCACGATGGCCTTCCAGCCCGTCG
TCGATCTGGTGAATCGCCGCATCGACGGGTACGAGGCCCTGGTTCGCGGCCCCGGGGGCGAGGGCGCGCCGAGCGTCCTG
GCGCAGGTCACGGAGGAAAACCTGTATGCTTTCGATCAGGCGTGCCGCACCCGGGCCATCCGGCTGGCGTCCCGGCTTGG
GCTGGACCGCCGCCTGAGCATCAATTTCCTGCCCAACGCCGTCTATAACCCGCGCGCCTGCATCCAGGCGACGCTGCAGG
CCGCCCGCGAAAGCGATTTTCCCCTGGAAAACCTGACCTTCGAGATCCTCGAGACCGAACATCTGACCGAGACGAAGCAC
CTGCAGGACATCATCGGCGAATATCATCACCATGGCTTCAAGGTCGCCCTGGACGATTTCCCGACCGGACAGTCCGGGCT
GATGCGGCTGGCCGATCTCAAGCCCGACATCATCAAGATCGACCGGGCCCTGGTACAGGATTGCGACCGGGACCAGGTGC
GCCGCACGATCATCGCCGGCATCATACGAATTGCCACCGATCTGGGCGTCAAGGTCGTGATCGAGGGCGTGGAGCGTCTT
GAGGAGGTGCGGGCCCTGCACGCCGTGGGCGGCCGCTTCTTCCAGGGGTTCTTCTTCGCACGGCCGCTGTTCGAGCAGAT
CGCGCGGGACGGCGATATCGACTGGCCCGTCATGGCCTGA

Upstream 100 bases:

>100_bases
CCGATGCCGATCCGCGAAAGATCATCACGGTTGCGGACACGCTACACGGATTTCCCGCGACTGGCCATGCGGGGCGTCCC
GGATAGCGAAGCGGCTGGAT

Downstream 100 bases:

>100_bases
CGGACGCCCCCGGCCACCGGGAACGGCGTGGCGGGCGGTGACCGGACGTCACCGGGGACGACCGACAAGGCGGCTCCCGC
GTCGGCGGGGCGTGGCCTAC

Product: hypothetical protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MGCFPGPTCDSHEFEYPRPEPPMTASPPSSRAPCQACHDHQDLFPFTMAFQPVVDLVNRRIDGYEALVRGPGGEGAPSVL
AQVTEENLYAFDQACRTRAIRLASRLGLDRRLSINFLPNAVYNPRACIQATLQAARESDFPLENLTFEILETEHLTETKH
LQDIIGEYHHHGFKVALDDFPTGQSGLMRLADLKPDIIKIDRALVQDCDRDQVRRTIIAGIIRIATDLGVKVVIEGVERL
EEVRALHAVGGRFFQGFFFARPLFEQIARDGDIDWPVMA

Sequences:

>Translated_279_residues
MGCFPGPTCDSHEFEYPRPEPPMTASPPSSRAPCQACHDHQDLFPFTMAFQPVVDLVNRRIDGYEALVRGPGGEGAPSVL
AQVTEENLYAFDQACRTRAIRLASRLGLDRRLSINFLPNAVYNPRACIQATLQAARESDFPLENLTFEILETEHLTETKH
LQDIIGEYHHHGFKVALDDFPTGQSGLMRLADLKPDIIKIDRALVQDCDRDQVRRTIIAGIIRIATDLGVKVVIEGVERL
EEVRALHAVGGRFFQGFFFARPLFEQIARDGDIDWPVMA
>Mature_278_residues
GCFPGPTCDSHEFEYPRPEPPMTASPPSSRAPCQACHDHQDLFPFTMAFQPVVDLVNRRIDGYEALVRGPGGEGAPSVLA
QVTEENLYAFDQACRTRAIRLASRLGLDRRLSINFLPNAVYNPRACIQATLQAARESDFPLENLTFEILETEHLTETKHL
QDIIGEYHHHGFKVALDDFPTGQSGLMRLADLKPDIIKIDRALVQDCDRDQVRRTIIAGIIRIATDLGVKVVIEGVERLE
EVRALHAVGGRFFQGFFFARPLFEQIARDGDIDWPVMA

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=231, Percent_Identity=31.6017316017316, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790496, Length=252, Percent_Identity=29.7619047619048, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788502, Length=230, Percent_Identity=29.5652173913043, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87081845, Length=237, Percent_Identity=29.535864978903, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081980, Length=228, Percent_Identity=28.0701754385965, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87082096, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=29.2993630573248, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788849, Length=153, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081921, Length=237, Percent_Identity=28.6919831223629, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081743, Length=224, Percent_Identity=27.6785714285714, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI226510982, Length=241, Percent_Identity=27.3858921161826, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786507, Length=119, Percent_Identity=31.9327731092437, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787055, Length=233, Percent_Identity=29.1845493562232, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 31268; Mature: 31137

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCFPGPTCDSHEFEYPRPEPPMTASPPSSRAPCQACHDHQDLFPFTMAFQPVVDLVNRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCC
IDGYEALVRGPGGEGAPSVLAQVTEENLYAFDQACRTRAIRLASRLGLDRRLSINFLPNA
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
VYNPRACIQATLQAARESDFPLENLTFEILETEHLTETKHLQDIIGEYHHHGFKVALDDF
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
PTGQSGLMRLADLKPDIIKIDRALVQDCDRDQVRRTIIAGIIRIATDLGVKVVIEGVERL
CCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
EEVRALHAVGGRFFQGFFFARPLFEQIARDGDIDWPVMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GCFPGPTCDSHEFEYPRPEPPMTASPPSSRAPCQACHDHQDLFPFTMAFQPVVDLVNRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCC
IDGYEALVRGPGGEGAPSVLAQVTEENLYAFDQACRTRAIRLASRLGLDRRLSINFLPNA
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
VYNPRACIQATLQAARESDFPLENLTFEILETEHLTETKHLQDIIGEYHHHGFKVALDDF
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
PTGQSGLMRLADLKPDIIKIDRALVQDCDRDQVRRTIIAGIIRIATDLGVKVVIEGVERL
CCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
EEVRALHAVGGRFFQGFFFARPLFEQIARDGDIDWPVMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]