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The map label for this gene is puuE [H]

Identifier: 162146108

GI number: 162146108

Start: 286765

End: 288138

Strand: Reverse

Name: puuE [H]

Synonym: GDI_0277

Alternate gene names: 162146108

Gene position: 288138-286765 (Counterclockwise)

Preceding gene: 162146109

Following gene: 162146101

Centisome position: 7.31

GC content: 72.34

Gene sequence:

>1374_bases
GTGACCGGACGTCACCGGGGACGACCGACAAGGCGGCTCCCGCGTCGGCGGGGCGTGGCCTACATCTGTCGGGCGGATTC
ACGCCAGACGAGGACAGGCACGATGTCATCCACCAATTCCGATCTTCAGGCCCGGCGCGAGGCCGCGGTGCCGCGCGGCG
TCGCATCGGCCACCGCCATCCATGCCGCGCGGGCGGAGAATGCCGAGCTGTGGGATGTCGAGGGGCGGCGCTATATCGAC
TTCGCCGGCGGCATCGCGGTGCTGAATGTCGGCCATCGCCACCCCCGTGTGATGGAGGCGGTGCGGCGGCAGGCGGAGCT
GTTCACCCACACCGCGTTCCAGGTCGCGCCGTATGAATCCTACATCGCCCTGGCCGAGCGGCTGAACGGGCTGGCGCCGT
TTCCCGGCCCGGCGAAGACCATCCTGTTTTCGACCGGGGCCGAGGCGACCGAGAACGCCGTGAAGATCGCCCGCGCGGCG
ACCGGGCGCAGCGGCGTCGTGGCCTTTACCGGCGCCTTCCACGGCCGGTCGCTGCTGGCGTCGGCGCTGACCGGCAAGGT
CCGGCCGTACAAGGCGCCGTTCGGCAGCATGCCGGCGGGCGTCTATCACGTGCCGTTCCCGGCCGGGGAGGTCACGGTCG
AGGACAGCCTGAAGGCGCTGGATTTCCTGTTCGCGGCCGATATCGATCCGGCGCAGGTGGCCGCCATCATCATCGAGCCG
GTACAGGGCGAGGGCGGATTCCGTCCGACCCCCGCCGCCCTGCTGCGGGCGCTGCGCGCGGTCTGCGATACGCACGGGAT
CGTGCTGATCGCCGACGAGGTGCAGACCGGCTTCGCGCGGACCGGGCGGCTGTTCGGCATCGAACTGTCGGGCGTGGCGC
CGGACCTGGTCTGTATCGCGAAATCGCTGGGCGGCGGCTTTCCGCTGTCGGGCGTGATCGGCCGCGCCGCGCTGATGGAT
GCGGTGGGGCCCGGCGGGCTGGGCGGGACCTATGCCGGGGCGCCGATCGCCTGCGCGGCGGCGCTGGCGGTCCTGGACGT
CATTGCCGAAGAGGGGCTGGTGGAGCGGGCGAACGCGATCGGCGCCCTGATCCGCGACCGGCTGGAGGGGATGCGCGGCC
GGCGCGGCCTGACGCCGATCAGCGCGCCGCGCGGACCGGGAGCCATGATCGGCTTCGACATCCTGGAGGGGCCGGACGGG
CCGGTGCGCGTCGGCGGGGCCGCCGCGATCTGCGCCCGCGCCGCCGAACGGGGGCTGATCCTGCTGTCATGCGGCATCCA
GGGCGAGACGGTGCGCATCCTGGTGCCGCTGACGGCGCCGGACAGCCTGATCGAGGAAGGGCTGGCGGTCATCGAGCAGG
CGCTGGCGCTTTAA

Upstream 100 bases:

>100_bases
GCACGGCCGCTGTTCGAGCAGATCGCGCGGGACGGCGATATCGACTGGCCCGTCATGGCCTGACGGACGCCCCCGGCCAC
CGGGAACGGCGTGGCGGGCG

Downstream 100 bases:

>100_bases
AGGCCGGGTCCTGGCCCTGGTCCGGGCCCTGGCCCTACGCCGCCCGCCCGGCGGCGGCGGGGATCAGGGCTTCGACCTGC
CGCCGCGTATTGACCACGTC

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTGRHRGRPTRRLPRRRGVAYICRADSRQTRTGTMSSTNSDLQARREAAVPRGVASATAIHAARAENAELWDVEGRRYID
FAGGIAVLNVGHRHPRVMEAVRRQAELFTHTAFQVAPYESYIALAERLNGLAPFPGPAKTILFSTGAEATENAVKIARAA
TGRSGVVAFTGAFHGRSLLASALTGKVRPYKAPFGSMPAGVYHVPFPAGEVTVEDSLKALDFLFAADIDPAQVAAIIIEP
VQGEGGFRPTPAALLRALRAVCDTHGIVLIADEVQTGFARTGRLFGIELSGVAPDLVCIAKSLGGGFPLSGVIGRAALMD
AVGPGGLGGTYAGAPIACAAALAVLDVIAEEGLVERANAIGALIRDRLEGMRGRRGLTPISAPRGPGAMIGFDILEGPDG
PVRVGGAAAICARAAERGLILLSCGIQGETVRILVPLTAPDSLIEEGLAVIEQALAL

Sequences:

>Translated_457_residues
MTGRHRGRPTRRLPRRRGVAYICRADSRQTRTGTMSSTNSDLQARREAAVPRGVASATAIHAARAENAELWDVEGRRYID
FAGGIAVLNVGHRHPRVMEAVRRQAELFTHTAFQVAPYESYIALAERLNGLAPFPGPAKTILFSTGAEATENAVKIARAA
TGRSGVVAFTGAFHGRSLLASALTGKVRPYKAPFGSMPAGVYHVPFPAGEVTVEDSLKALDFLFAADIDPAQVAAIIIEP
VQGEGGFRPTPAALLRALRAVCDTHGIVLIADEVQTGFARTGRLFGIELSGVAPDLVCIAKSLGGGFPLSGVIGRAALMD
AVGPGGLGGTYAGAPIACAAALAVLDVIAEEGLVERANAIGALIRDRLEGMRGRRGLTPISAPRGPGAMIGFDILEGPDG
PVRVGGAAAICARAAERGLILLSCGIQGETVRILVPLTAPDSLIEEGLAVIEQALAL
>Mature_456_residues
TGRHRGRPTRRLPRRRGVAYICRADSRQTRTGTMSSTNSDLQARREAAVPRGVASATAIHAARAENAELWDVEGRRYIDF
AGGIAVLNVGHRHPRVMEAVRRQAELFTHTAFQVAPYESYIALAERLNGLAPFPGPAKTILFSTGAEATENAVKIARAAT
GRSGVVAFTGAFHGRSLLASALTGKVRPYKAPFGSMPAGVYHVPFPAGEVTVEDSLKALDFLFAADIDPAQVAAIIIEPV
QGEGGFRPTPAALLRALRAVCDTHGIVLIADEVQTGFARTGRLFGIELSGVAPDLVCIAKSLGGGFPLSGVIGRAALMDA
VGPGGLGGTYAGAPIACAAALAVLDVIAEEGLVERANAIGALIRDRLEGMRGRRGLTPISAPRGPGAMIGFDILEGPDGP
VRVGGAAAICARAAERGLILLSCGIQGETVRILVPLTAPDSLIEEGLAVIEQALAL

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=455, Percent_Identity=30.989010989011, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI13994255, Length=357, Percent_Identity=33.0532212885154, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI284507298, Length=323, Percent_Identity=32.1981424148607, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI37574042, Length=427, Percent_Identity=28.5714285714286, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI226442705, Length=427, Percent_Identity=27.8688524590164, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI226442709, Length=397, Percent_Identity=28.463476070529, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI24119277, Length=395, Percent_Identity=26.3291139240506, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI188536080, Length=455, Percent_Identity=24.3956043956044, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI38679950, Length=455, Percent_Identity=24.3956043956044, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI38679946, Length=455, Percent_Identity=24.3956043956044, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1787560, Length=421, Percent_Identity=53.4441805225653, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1789016, Length=426, Percent_Identity=52.112676056338, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1789759, Length=399, Percent_Identity=34.3358395989975, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788044, Length=392, Percent_Identity=36.2244897959184, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI145693181, Length=339, Percent_Identity=35.9882005899705, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1786349, Length=341, Percent_Identity=33.7243401759531, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786991, Length=318, Percent_Identity=30.8176100628931, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25144271, Length=404, Percent_Identity=30.9405940594059, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI32564660, Length=424, Percent_Identity=29.2452830188679, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI71992977, Length=423, Percent_Identity=28.8416075650118, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI25144274, Length=292, Percent_Identity=30.8219178082192, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17541228, Length=380, Percent_Identity=26.8421052631579, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71981843, Length=158, Percent_Identity=31.6455696202532, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324432, Length=405, Percent_Identity=32.5925925925926, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6323470, Length=418, Percent_Identity=30.3827751196172, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6324386, Length=322, Percent_Identity=24.223602484472, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321456, Length=370, Percent_Identity=25.6756756756757, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI161085790, Length=455, Percent_Identity=30.1098901098901, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI21357415, Length=407, Percent_Identity=30.4668304668305, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28574759, Length=455, Percent_Identity=30.7692307692308, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21356575, Length=354, Percent_Identity=32.2033898305085, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI281366494, Length=342, Percent_Identity=26.9005847953216, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24667139, Length=342, Percent_Identity=26.9005847953216, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24667143, Length=342, Percent_Identity=26.9005847953216, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 47559; Mature: 47428

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00606 B_KETOACYL_SYNTHASE ; PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRHRGRPTRRLPRRRGVAYICRADSRQTRTGTMSSTNSDLQARREAAVPRGVASATAI
CCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
HAARAENAELWDVEGRRYIDFAGGIAVLNVGHRHPRVMEAVRRQAELFTHTAFQVAPYES
HHHHCCCCEEEECCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCHHH
YIALAERLNGLAPFPGPAKTILFSTGAEATENAVKIARAATGRSGVVAFTGAFHGRSLLA
HHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
SALTGKVRPYKAPFGSMPAGVYHVPFPAGEVTVEDSLKALDFLFAADIDPAQVAAIIIEP
HHHHCCCCCCCCCCCCCCCCEEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHEEEEEEEE
VQGEGGFRPTPAALLRALRAVCDTHGIVLIADEVQTGFARTGRLFGIELSGVAPDLVCIA
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHCHHHCCCEEEEEECCCCHHHHHHH
KSLGGGFPLSGVIGRAALMDAVGPGGLGGTYAGAPIACAAALAVLDVIAEEGLVERANAI
HHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
GALIRDRLEGMRGRRGLTPISAPRGPGAMIGFDILEGPDGPVRVGGAAAICARAAERGLI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECHHHHHHHHHHHCCEE
LLSCGIQGETVRILVPLTAPDSLIEEGLAVIEQALAL
EEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGRHRGRPTRRLPRRRGVAYICRADSRQTRTGTMSSTNSDLQARREAAVPRGVASATAI
CCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
HAARAENAELWDVEGRRYIDFAGGIAVLNVGHRHPRVMEAVRRQAELFTHTAFQVAPYES
HHHHCCCCEEEECCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCHHH
YIALAERLNGLAPFPGPAKTILFSTGAEATENAVKIARAATGRSGVVAFTGAFHGRSLLA
HHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
SALTGKVRPYKAPFGSMPAGVYHVPFPAGEVTVEDSLKALDFLFAADIDPAQVAAIIIEP
HHHHCCCCCCCCCCCCCCCCEEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHEEEEEEEE
VQGEGGFRPTPAALLRALRAVCDTHGIVLIADEVQTGFARTGRLFGIELSGVAPDLVCIA
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHCHHHCCCEEEEEECCCCHHHHHHH
KSLGGGFPLSGVIGRAALMDAVGPGGLGGTYAGAPIACAAALAVLDVIAEEGLVERANAI
HHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
GALIRDRLEGMRGRRGLTPISAPRGPGAMIGFDILEGPDGPVRVGGAAAICARAAERGLI
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEECHHHHHHHHHHHCCEE
LLSCGIQGETVRILVPLTAPDSLIEEGLAVIEQALAL
EEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]