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The map label for this gene is gcvA [H]

Identifier: 162146100

GI number: 162146100

Start: 281344

End: 282369

Strand: Reverse

Name: gcvA [H]

Synonym: GDI_0269

Alternate gene names: 162146100

Gene position: 282369-281344 (Counterclockwise)

Preceding gene: 162146101

Following gene: 162146099

Centisome position: 7.16

GC content: 73.78

Gene sequence:

>1026_bases
GTGGTGACAAAAAGGCACCGGTATGCGCACATGGTCCCCCGACCCGAGCATCCGAGACTGCGGAGCCCCATGTCCGACCG
GCGCCCCCTTCCCCCGTTCGCCCCGTTGCGGGCCTTCGACGCCTTCGGCCGGACCGGCGGCATTCGCCGGGCCGCGACCT
GGCTGGGCGTGGACCACGCGGTGGTCAGCCGGCAGGTGCGGGCGCTGGAGCAATGGATCGGGGTGCCATTGCTGGACCGG
TCCCCCGGCCAGGGCCAGGGAACGCTGAACGCGGCGGGCCGGGCCTATCACGCGCGGATCGCGGCGGCGCTGGACGAGAT
CGCGAGCGCCACCGAGGCGCTGCGCCTGGGCGACGCGTCGCACCTGAGCGTCTGGTGCGTGCCGGGCTTCGCCTTCCACT
GGCTGGTGCGACGGCTGGGCGCGTTTCGTGCCGAACATCCCGAGATCGACCTGGTGCTGCGCCCGACCGACGCGCGGGCG
TCGTTCTGGTCCGACGGGGTGGATGCGGATATCCGCTATCGGCTGGACGGCGCGCCCGGCGGTGCGCCCGATGGCGCGTA
CGACACCCCGGGCATCCGCCGGGTGGATCTGGCGCGGCCGGTGGTGTTCCCGGTCGCCAGCCCCGATTTCATCCGCCGCG
CGGGCACGATCCGCACGATCGGCGACCTGCCGGCGCTGCCGCTGCTGGCCGAGGAGGACAGCCACGAATGGCTGGCATGG
TTCGCGCGGCAGGGCGTGGCCGTGCCCCGCATCACCCCGGCGGCGCGGCTGTGGCATGCCCATGTGGTGCTGGCGGCGGC
ACGTGAGGGACAGGGCGTGGCGCTGGCCAATCCGTTCCTGGTGGCGGACGACCTGGAGCGCGGTACGCTGGTGCGGCTGG
GCCCGCCGGGCGCGCCGTTTCCGGGCGTGGAGATCGGGGCCTATACGCTGTTCGTGCCGGAAACCCGGCGGGACCGGCCC
GCCCTGGTGCGCTTCAGCACCTGGCTGTCGCGCACCGCCGGCGCGTTCCTGCCGCCGGAGCGTTAA

Upstream 100 bases:

>100_bases
TGTTGCCGGCAAGGCACAGTTTCTGTCCCCCCGCTTCCGGCACACCGGATTTTTCTTAGTTGACCGTAACGTTGCGTGGA
AGGCGCCTTCGCGTGCGGCG

Downstream 100 bases:

>100_bases
ATCCCCTGCGCCGGCACGGGAAATCTGGTTATGATCGCGCGATTCCAGACTGGGACACAGAAAATGGGTATGCCAATCGG
TGACGGGGTGCCGGCCCCCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MVTKRHRYAHMVPRPEHPRLRSPMSDRRPLPPFAPLRAFDAFGRTGGIRRAATWLGVDHAVVSRQVRALEQWIGVPLLDR
SPGQGQGTLNAAGRAYHARIAAALDEIASATEALRLGDASHLSVWCVPGFAFHWLVRRLGAFRAEHPEIDLVLRPTDARA
SFWSDGVDADIRYRLDGAPGGAPDGAYDTPGIRRVDLARPVVFPVASPDFIRRAGTIRTIGDLPALPLLAEEDSHEWLAW
FARQGVAVPRITPAARLWHAHVVLAAAREGQGVALANPFLVADDLERGTLVRLGPPGAPFPGVEIGAYTLFVPETRRDRP
ALVRFSTWLSRTAGAFLPPER

Sequences:

>Translated_341_residues
MVTKRHRYAHMVPRPEHPRLRSPMSDRRPLPPFAPLRAFDAFGRTGGIRRAATWLGVDHAVVSRQVRALEQWIGVPLLDR
SPGQGQGTLNAAGRAYHARIAAALDEIASATEALRLGDASHLSVWCVPGFAFHWLVRRLGAFRAEHPEIDLVLRPTDARA
SFWSDGVDADIRYRLDGAPGGAPDGAYDTPGIRRVDLARPVVFPVASPDFIRRAGTIRTIGDLPALPLLAEEDSHEWLAW
FARQGVAVPRITPAARLWHAHVVLAAAREGQGVALANPFLVADDLERGTLVRLGPPGAPFPGVEIGAYTLFVPETRRDRP
ALVRFSTWLSRTAGAFLPPER
>Mature_341_residues
MVTKRHRYAHMVPRPEHPRLRSPMSDRRPLPPFAPLRAFDAFGRTGGIRRAATWLGVDHAVVSRQVRALEQWIGVPLLDR
SPGQGQGTLNAAGRAYHARIAAALDEIASATEALRLGDASHLSVWCVPGFAFHWLVRRLGAFRAEHPEIDLVLRPTDARA
SFWSDGVDADIRYRLDGAPGGAPDGAYDTPGIRRVDLARPVVFPVASPDFIRRAGTIRTIGDLPALPLLAEEDSHEWLAW
FARQGVAVPRITPAARLWHAHVVLAAAREGQGVALANPFLVADDLERGTLVRLGPPGAPFPGVEIGAYTLFVPETRRDRP
ALVRFSTWLSRTAGAFLPPER

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=315, Percent_Identity=30.7936507936508, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786448, Length=299, Percent_Identity=29.4314381270903, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786401, Length=281, Percent_Identity=26.3345195729537, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081978, Length=271, Percent_Identity=28.7822878228782, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=307, Percent_Identity=23.1270358306189, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37380; Mature: 37380

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTKRHRYAHMVPRPEHPRLRSPMSDRRPLPPFAPLRAFDAFGRTGGIRRAATWLGVDHA
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCHHHH
VVSRQVRALEQWIGVPLLDRSPGQGQGTLNAAGRAYHARIAAALDEIASATEALRLGDAS
HHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
HLSVWCVPGFAFHWLVRRLGAFRAEHPEIDLVLRPTDARASFWSDGVDADIRYRLDGAPG
CEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCCCCEEEEEECCCCC
GAPDGAYDTPGIRRVDLARPVVFPVASPDFIRRAGTIRTIGDLPALPLLAEEDSHEWLAW
CCCCCCCCCCCCEEEECCCCEEEECCCHHHHHHCCCEEECCCCCCCCEEECCCCHHHHHH
FARQGVAVPRITPAARLWHAHVVLAAAREGQGVALANPFLVADDLERGTLVRLGPPGAPF
HHHCCCCCCCCCHHHHHHHHHEEEEECCCCCCEEEECCEEEECCCCCCCEEEECCCCCCC
PGVEIGAYTLFVPETRRDRPALVRFSTWLSRTAGAFLPPER
CCCEECEEEEEECCCCCCCCCEEHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVTKRHRYAHMVPRPEHPRLRSPMSDRRPLPPFAPLRAFDAFGRTGGIRRAATWLGVDHA
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCHHHH
VVSRQVRALEQWIGVPLLDRSPGQGQGTLNAAGRAYHARIAAALDEIASATEALRLGDAS
HHHHHHHHHHHHHCCCEECCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
HLSVWCVPGFAFHWLVRRLGAFRAEHPEIDLVLRPTDARASFWSDGVDADIRYRLDGAPG
CEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCCCCEEEEEECCCCC
GAPDGAYDTPGIRRVDLARPVVFPVASPDFIRRAGTIRTIGDLPALPLLAEEDSHEWLAW
CCCCCCCCCCCCEEEECCCCEEEECCCHHHHHHCCCEEECCCCCCCCEEECCCCHHHHHH
FARQGVAVPRITPAARLWHAHVVLAAAREGQGVALANPFLVADDLERGTLVRLGPPGAPF
HHHCCCCCCCCCHHHHHHHHHEEEEECCCCCCEEEECCEEEECCCCCCCEEEECCCCCCC
PGVEIGAYTLFVPETRRDRPALVRFSTWLSRTAGAFLPPER
CCCEECEEEEEECCCCCCCCCEEHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]