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The map label for this gene is trpI [H]

Identifier: 162146048

GI number: 162146048

Start: 233000

End: 233863

Strand: Reverse

Name: trpI [H]

Synonym: GDI_0216

Alternate gene names: 162146048

Gene position: 233863-233000 (Counterclockwise)

Preceding gene: 162146052

Following gene: 162146046

Centisome position: 5.93

GC content: 70.95

Gene sequence:

>864_bases
ATGAAGCGATCCGACATCCCGTCGCTGGACGATCTGCGCGCCTTCGCGGCGGTAGTGCGCCTGGGCTCGGTCCGGCCGGC
GGCCATGGAACTGGCCCTGACGCATGGGGCGGTCAGCCGCCGGGTGTCCAAGCTGCCCGCCGAACTGAAACTGCGATTGC
TGGAACCGGACGGGCGCGGCGTGCGGCCGACCCGGGACGGCGAGCGGCTTGCCCGCGCGGCGAGCGACGCGCTGGGCGGG
ATCGCGGCGGCGCTGGCCGAGATCAGGTCGCCCTCCACCGCGCCGCCGATCGTGCTGTCCTGCGAGCGGTCGCTGGCGAT
GCGCTGGCTGATCCCGCGCCTGTCGGACTTCCAGGACCGGCATCCCGGCATCGACGTGCATCTTTCGACCGGTGGCGGGG
CGCTGGATTTCGCGCGGGAACGGATTACGCTGGCGATCCGGCGCCTCGATTTCCCGCTCGATCCCGACTGGGCCGTCACC
ACGCTCGTGCCGGAAACGGTCGGGCCGGTCATGCGGCCCGACATGGCGGAGCGGTTCCGCAGGGGCGACTACCTGGCGCT
GGGATCGCGCACCCGGCCCGAGGCATGGAGGCACTGGCTGGCCTCCCGGCCCGACAGTGCGGCGCCCCGCGATACACGCC
TGTTCGACCATCATTTCATGATGCTGGAAGCCGCCGCCAGCGGCCTGGGTGTCGCCCTGTCACCCCGGATCATCGCGGCG
GACGATGTCGCGACCGGTCGATTGCTGGCGCCGGCCGGATTCGATGCGGACGGAACGTCCTACGGGCTGATCCGGCCCAG
GGGGATGGACGTGACGGAACAGGTCGAGGCGCTGGAACAATGGATCGTGCAGCAGGCGGGATAA

Upstream 100 bases:

>100_bases
TGTGGCAGGAGGAGGGTGCGGTCATGATGGTGAGAATAGCGGAACGGTTCCGGAGGAAAATCGATATGAACTGACCTGTT
CCGGTGATAAAAACTGACCA

Downstream 100 bases:

>100_bases
TTCGCGCCCTACTGGTATCTGATCCGGCCCGCCGTCACCGCCCGATGCCGGGTATTTTCGCGATTTCGCTGCCGAGAAAA
TCGGCGCAGAGACGCACCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKRSDIPSLDDLRAFAAVVRLGSVRPAAMELALTHGAVSRRVSKLPAELKLRLLEPDGRGVRPTRDGERLARAASDALGG
IAAALAEIRSPSTAPPIVLSCERSLAMRWLIPRLSDFQDRHPGIDVHLSTGGGALDFARERITLAIRRLDFPLDPDWAVT
TLVPETVGPVMRPDMAERFRRGDYLALGSRTRPEAWRHWLASRPDSAAPRDTRLFDHHFMMLEAAASGLGVALSPRIIAA
DDVATGRLLAPAGFDADGTSYGLIRPRGMDVTEQVEALEQWIVQQAG

Sequences:

>Translated_287_residues
MKRSDIPSLDDLRAFAAVVRLGSVRPAAMELALTHGAVSRRVSKLPAELKLRLLEPDGRGVRPTRDGERLARAASDALGG
IAAALAEIRSPSTAPPIVLSCERSLAMRWLIPRLSDFQDRHPGIDVHLSTGGGALDFARERITLAIRRLDFPLDPDWAVT
TLVPETVGPVMRPDMAERFRRGDYLALGSRTRPEAWRHWLASRPDSAAPRDTRLFDHHFMMLEAAASGLGVALSPRIIAA
DDVATGRLLAPAGFDADGTSYGLIRPRGMDVTEQVEALEQWIVQQAG
>Mature_287_residues
MKRSDIPSLDDLRAFAAVVRLGSVRPAAMELALTHGAVSRRVSKLPAELKLRLLEPDGRGVRPTRDGERLARAASDALGG
IAAALAEIRSPSTAPPIVLSCERSLAMRWLIPRLSDFQDRHPGIDVHLSTGGGALDFARERITLAIRRLDFPLDPDWAVT
TLVPETVGPVMRPDMAERFRRGDYLALGSRTRPEAWRHWLASRPDSAAPRDTRLFDHHFMMLEAAASGLGVALSPRIIAA
DDVATGRLLAPAGFDADGTSYGLIRPRGMDVTEQVEALEQWIVQQAG

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786448, Length=284, Percent_Identity=26.7605633802817, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788706, Length=259, Percent_Identity=27.4131274131274, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=26.4406779661017, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786401, Length=206, Percent_Identity=25.7281553398058, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=181, Percent_Identity=30.939226519337, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31285; Mature: 31285

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSDIPSLDDLRAFAAVVRLGSVRPAAMELALTHGAVSRRVSKLPAELKLRLLEPDGRG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCC
VRPTRDGERLARAASDALGGIAAALAEIRSPSTAPPIVLSCERSLAMRWLIPRLSDFQDR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHC
HPGIDVHLSTGGGALDFARERITLAIRRLDFPLDPDWAVTTLVPETVGPVMRPDMAERFR
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCCCCCHHHHHC
RGDYLALGSRTRPEAWRHWLASRPDSAAPRDTRLFDHHFMMLEAAASGLGVALSPRIIAA
CCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEE
DDVATGRLLAPAGFDADGTSYGLIRPRGMDVTEQVEALEQWIVQQAG
CCCCCCCEECCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRSDIPSLDDLRAFAAVVRLGSVRPAAMELALTHGAVSRRVSKLPAELKLRLLEPDGRG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCC
VRPTRDGERLARAASDALGGIAAALAEIRSPSTAPPIVLSCERSLAMRWLIPRLSDFQDR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHC
HPGIDVHLSTGGGALDFARERITLAIRRLDFPLDPDWAVTTLVPETVGPVMRPDMAERFR
CCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHCCCCCCCHHHHHC
RGDYLALGSRTRPEAWRHWLASRPDSAAPRDTRLFDHHFMMLEAAASGLGVALSPRIIAA
CCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEE
DDVATGRLLAPAGFDADGTSYGLIRPRGMDVTEQVEALEQWIVQQAG
CCCCCCCEECCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]