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The map label for this gene is gatA [H]

Identifier: 162146028

GI number: 162146028

Start: 210069

End: 211487

Strand: Direct

Name: gatA [H]

Synonym: GDI_0196

Alternate gene names: 162146028

Gene position: 210069-211487 (Clockwise)

Preceding gene: 162146027

Following gene: 162146031

Centisome position: 5.33

GC content: 67.44

Gene sequence:

>1419_bases
ATGACCGCGCGGGATCTGGCCGATCTGAGCCTTGTGGATGCGGTGGACGCGGTCGCTATGCGCGAGACGACCTCCATGGC
GCTTCTGGAGGCCTGTCTGGCCCGGCTTGACGCCGCCGAGGAGCGGATCAACGCCACCATCTGGCTCGATCGGGAGGGGG
CTTTCCGCGCGGCGGAGGCCGCCGACAAGGCCGTGGCCGCGGGCGCTGCGCTGGGGCTGCTGCACGGCCTGCCGCTGGCG
CACAAGGACATGTACTATCAGGCCGGCAGGCCGTGCACCTGCGGATCGGTGATCCGCAAGGATTTCGTCCCCACGGTGAC
GGCGACCGTGGTCGAGCGCATGGCCGCGGCCGGGGCCTATGCCTATGGCGGCCTGAACATGGCCGAATTCGCAATGAACC
CGACGGGTCACAACGCGCATTACGGCGACTGTCATAACCCCTGGAACCTTCCCTATATCACCGGTGGCTCGTCCAGCGGA
TCGGGGGCGGCGGTCGCCTCGCGCTGCACCTATGGCGCGTTGGGGTCCGATACGGGTGGTTCCATCCGTCTGCCGGCGGC
TGCCTGTGGCGTGACGGGCCTGAAGCCGACACAGACGCGGGTCAGCCGCGCGGGGGTGATGCCGCTTTCCTTTTCGGCGG
ACAATGTGGGCCCCCTGACGCGCACGGTGCGGGATTGTGCCCGCATGATGAAGGTCATCGCCGGGCAGGACAGCCGCGAC
CCGACCAGTTCCGCCGAGCCCGTACCGGATTATGAGGCCGCCCTGGACGGCGATCTGCGGGGCCTGCGGGTCGGCATTCC
GACCACTTATTTTCTGGATGGCGCCGATGCTCCGGTTGTCGCCGCATTCGAGGCCGCGGTAGCTGTCCTGGCCGGACGCG
GCGCAACCGTAATGCGGCTGCCGCTGCCGCTGATGGATGCGGTTTCCGTCTATGCCGGTGTTCTCCTCCGTGTCGAAGCC
GCGGCGATCCATGCCGAATGGATGCGCGAGCGCCCCCAGGACTATTCCGTACAGCTCAGCGCGCGGCTGTTTGGCGGCAA
TGCCATTCCCGCTCCTTATTACGTGGAGGCGTTGAGCCGGCGCGGCCCGATCCTCAAGGCCTTCGCGGATGAGGTCTTCG
GGCAGGTCGATGTCCTGGCGACGCCCACGATCCGCACCTGCCTGCCGACCCTGGCCGAGACCAATATCGACCAGGGGTCG
CCCGCTTCCGTGCAGGCGTTCATGGCCGTGTCCGCGAATACGCGACCGTTCAACTATCTGGGCCTGCCGGCAATCAGCGT
GAATTGCGGTTTCGACCCGAACGGTTGCCCCATCGGCCTGCAGATCGCCGGCCGACCGTTCGCCGAGGCGCGCGTGATGA
CGGTTGCCGATGCCTACCAGCGCGACACGGACTGGCACACCAGACAGCCGCCTCTCTGA

Upstream 100 bases:

>100_bases
AACTGGCGGTGACGATCTCCGCGTTCGAGGCGATGCGGGGGCAACTGCGGTTCGAGGATGAACCGTCGAGCTTCGAAGCA
GCGCTGCAGGCGGCAAAGCA

Downstream 100 bases:

>100_bases
ATCCTGCCTGTCGGAACGGACTCATCGGCCGATGCGTCCGTTCCGTCATGTCTGTGAACCGGCACTGGCTGCCTCGGGGG
CAGGCGGTCTTTCGACCGCC

Product: glutamyl-tRNA(Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTARDLADLSLVDAVDAVAMRETTSMALLEACLARLDAAEERINATIWLDREGAFRAAEAADKAVAAGAALGLLHGLPLA
HKDMYYQAGRPCTCGSVIRKDFVPTVTATVVERMAAAGAYAYGGLNMAEFAMNPTGHNAHYGDCHNPWNLPYITGGSSSG
SGAAVASRCTYGALGSDTGGSIRLPAAACGVTGLKPTQTRVSRAGVMPLSFSADNVGPLTRTVRDCARMMKVIAGQDSRD
PTSSAEPVPDYEAALDGDLRGLRVGIPTTYFLDGADAPVVAAFEAAVAVLAGRGATVMRLPLPLMDAVSVYAGVLLRVEA
AAIHAEWMRERPQDYSVQLSARLFGGNAIPAPYYVEALSRRGPILKAFADEVFGQVDVLATPTIRTCLPTLAETNIDQGS
PASVQAFMAVSANTRPFNYLGLPAISVNCGFDPNGCPIGLQIAGRPFAEARVMTVADAYQRDTDWHTRQPPL

Sequences:

>Translated_472_residues
MTARDLADLSLVDAVDAVAMRETTSMALLEACLARLDAAEERINATIWLDREGAFRAAEAADKAVAAGAALGLLHGLPLA
HKDMYYQAGRPCTCGSVIRKDFVPTVTATVVERMAAAGAYAYGGLNMAEFAMNPTGHNAHYGDCHNPWNLPYITGGSSSG
SGAAVASRCTYGALGSDTGGSIRLPAAACGVTGLKPTQTRVSRAGVMPLSFSADNVGPLTRTVRDCARMMKVIAGQDSRD
PTSSAEPVPDYEAALDGDLRGLRVGIPTTYFLDGADAPVVAAFEAAVAVLAGRGATVMRLPLPLMDAVSVYAGVLLRVEA
AAIHAEWMRERPQDYSVQLSARLFGGNAIPAPYYVEALSRRGPILKAFADEVFGQVDVLATPTIRTCLPTLAETNIDQGS
PASVQAFMAVSANTRPFNYLGLPAISVNCGFDPNGCPIGLQIAGRPFAEARVMTVADAYQRDTDWHTRQPPL
>Mature_471_residues
TARDLADLSLVDAVDAVAMRETTSMALLEACLARLDAAEERINATIWLDREGAFRAAEAADKAVAAGAALGLLHGLPLAH
KDMYYQAGRPCTCGSVIRKDFVPTVTATVVERMAAAGAYAYGGLNMAEFAMNPTGHNAHYGDCHNPWNLPYITGGSSSGS
GAAVASRCTYGALGSDTGGSIRLPAAACGVTGLKPTQTRVSRAGVMPLSFSADNVGPLTRTVRDCARMMKVIAGQDSRDP
TSSAEPVPDYEAALDGDLRGLRVGIPTTYFLDGADAPVVAAFEAAVAVLAGRGATVMRLPLPLMDAVSVYAGVLLRVEAA
AIHAEWMRERPQDYSVQLSARLFGGNAIPAPYYVEALSRRGPILKAFADEVFGQVDVLATPTIRTCLPTLAETNIDQGSP
ASVQAFMAVSANTRPFNYLGLPAISVNCGFDPNGCPIGLQIAGRPFAEARVMTVADAYQRDTDWHTRQPPL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=32.0079522862823, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI195972892, Length=238, Percent_Identity=33.1932773109244, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI166795287, Length=198, Percent_Identity=30.3030303030303, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=416, Percent_Identity=29.8076923076923, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17537465, Length=252, Percent_Identity=31.3492063492063, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556276, Length=309, Percent_Identity=28.1553398058252, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=309, Percent_Identity=28.1553398058252, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=288, Percent_Identity=26.7361111111111, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=276, Percent_Identity=24.2753623188406, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=336, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=189, Percent_Identity=37.5661375661376, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=393, Percent_Identity=28.498727735369, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648113, Length=456, Percent_Identity=33.3333333333333, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24644968, Length=514, Percent_Identity=26.6536964980545, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21356731, Length=505, Percent_Identity=25.3465346534653, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648435, Length=502, Percent_Identity=24.3027888446215, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648437, Length=502, Percent_Identity=24.3027888446215, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45550774, Length=502, Percent_Identity=24.3027888446215, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24652985, Length=233, Percent_Identity=29.1845493562232, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922090, Length=233, Percent_Identity=29.1845493562232, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24652981, Length=233, Percent_Identity=29.1845493562232, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24652983, Length=233, Percent_Identity=29.1845493562232, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=25.8517034068136, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648441, Length=437, Percent_Identity=24.2562929061785, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=437, Percent_Identity=24.2562929061785, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49654; Mature: 49523

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARDLADLSLVDAVDAVAMRETTSMALLEACLARLDAAEERINATIWLDREGAFRAAEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHH
ADKAVAAGAALGLLHGLPLAHKDMYYQAGRPCTCGSVIRKDFVPTVTATVVERMAAAGAY
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCH
AYGGLNMAEFAMNPTGHNAHYGDCHNPWNLPYITGGSSSGSGAAVASRCTYGALGSDTGG
HHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHCCCCCCCCCCCC
SIRLPAAACGVTGLKPTQTRVSRAGVMPLSFSADNVGPLTRTVRDCARMMKVIAGQDSRD
EEECCHHHCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
PTSSAEPVPDYEAALDGDLRGLRVGIPTTYFLDGADAPVVAAFEAAVAVLAGRGATVMRL
CCCCCCCCCCHHHHCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEE
PLPLMDAVSVYAGVLLRVEAAAIHAEWMRERPQDYSVQLSARLFGGNAIPAPYYVEALSR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHH
RGPILKAFADEVFGQVDVLATPTIRTCLPTLAETNIDQGSPASVQAFMAVSANTRPFNYL
CCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEE
GLPAISVNCGFDPNGCPIGLQIAGRPFAEARVMTVADAYQRDTDWHTRQPPL
CCCEEEEECCCCCCCCEEEEEECCCCCHHHHEEHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TARDLADLSLVDAVDAVAMRETTSMALLEACLARLDAAEERINATIWLDREGAFRAAEA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHH
ADKAVAAGAALGLLHGLPLAHKDMYYQAGRPCTCGSVIRKDFVPTVTATVVERMAAAGAY
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCH
AYGGLNMAEFAMNPTGHNAHYGDCHNPWNLPYITGGSSSGSGAAVASRCTYGALGSDTGG
HHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHCCCCCCCCCCCC
SIRLPAAACGVTGLKPTQTRVSRAGVMPLSFSADNVGPLTRTVRDCARMMKVIAGQDSRD
EEECCHHHCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
PTSSAEPVPDYEAALDGDLRGLRVGIPTTYFLDGADAPVVAAFEAAVAVLAGRGATVMRL
CCCCCCCCCCHHHHCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEE
PLPLMDAVSVYAGVLLRVEAAAIHAEWMRERPQDYSVQLSARLFGGNAIPAPYYVEALSR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHH
RGPILKAFADEVFGQVDVLATPTIRTCLPTLAETNIDQGSPASVQAFMAVSANTRPFNYL
CCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEE
GLPAISVNCGFDPNGCPIGLQIAGRPFAEARVMTVADAYQRDTDWHTRQPPL
CCCEEEEECCCCCCCCEEEEEECCCCCHHHHEEHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA