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The map label for this gene is estA [H]

Identifier: 162145981

GI number: 162145981

Start: 157878

End: 158546

Strand: Direct

Name: estA [H]

Synonym: GDI_0147

Alternate gene names: 162145981

Gene position: 157878-158546 (Clockwise)

Preceding gene: 162145979

Following gene: 162145983

Centisome position: 4.0

GC content: 63.38

Gene sequence:

>669_bases
ATGAAGCCGCTGAAAGCGCTGGAAGTCGAAACCGGGCGAAATCCCGTCGCGTCGATCATCCTTATTCACGGCCTGGGGGC
GAGTGGCCGGGATCTGGTGTCCATTGCTCAGGCGCTCGACCTGCGCTCCATCGGCGCGGTTCGGTTCATTTTTCCCAACG
CGCCCGTCCGGCCGGTTTCGGTCTGTGGTGGTGAGCGCATGCCGGCATGGTATGATCTTCTCGCGCCAGATTTGCTTCTG
CGGGAAGATGAGCCAGGGCTACGTGATGCCCAGACCTATCTGGCGAGCCTGATCGACCAGGAAGTCGCGCGCGGTATTCC
GTCCCGGCGCATCGTGATTGGCGGGTTTTCTCAGGGGTGTGCGATGTCGCTGATGACCGGGCTGCGCTATTCGTCGCCCC
TGGCTGGGATCGCCGGTCTGTCCGGCTATCTGCCGCTGCCCGGGCAAACGGGAAGGGAGGCAACGGAGGCCAACCGGGCA
ACGCCGGTTTTTCTGGCGCATGGTGAGGGCGACACGGTCGTGCCGCTGGCGGCGGCTCGCCTCGCACGGGACTGGCTACG
TGCCGAGGGGCATGACGTTGCTTGGTATGCCTACCCGATGACGCATGAGATCATAGGTGCGGAGATTGCCGATCTCAATG
CCTGGTTGGGGGAGCGTCTCGTAACCTAA

Upstream 100 bases:

>100_bases
GAGTTTCATGACAGCCTCCGCATGCTTGATAATGTACGATTATACCCGTACCATAGTTTCATCAAGCGTCATGAATGTCG
ATGAGAAAGGGGAGCAGAGG

Downstream 100 bases:

>100_bases
CGACCTTGGGCAAGGAGCAGCCGTGTGACTCACCTCCAGACTTTAATGGCCCATCTCGGTCTAAGTGGTATCAGGATTAG
AGTGCAGTTTCGATAGACAG

Product: acyl-protein thioesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKPLKALEVETGRNPVASIILIHGLGASGRDLVSIAQALDLRSIGAVRFIFPNAPVRPVSVCGGERMPAWYDLLAPDLLL
REDEPGLRDAQTYLASLIDQEVARGIPSRRIVIGGFSQGCAMSLMTGLRYSSPLAGIAGLSGYLPLPGQTGREATEANRA
TPVFLAHGEGDTVVPLAAARLARDWLRAEGHDVAWYAYPMTHEIIGAEIADLNAWLGERLVT

Sequences:

>Translated_222_residues
MKPLKALEVETGRNPVASIILIHGLGASGRDLVSIAQALDLRSIGAVRFIFPNAPVRPVSVCGGERMPAWYDLLAPDLLL
REDEPGLRDAQTYLASLIDQEVARGIPSRRIVIGGFSQGCAMSLMTGLRYSSPLAGIAGLSGYLPLPGQTGREATEANRA
TPVFLAHGEGDTVVPLAAARLARDWLRAEGHDVAWYAYPMTHEIIGAEIADLNAWLGERLVT
>Mature_222_residues
MKPLKALEVETGRNPVASIILIHGLGASGRDLVSIAQALDLRSIGAVRFIFPNAPVRPVSVCGGERMPAWYDLLAPDLLL
REDEPGLRDAQTYLASLIDQEVARGIPSRRIVIGGFSQGCAMSLMTGLRYSSPLAGIAGLSGYLPLPGQTGREATEANRA
TPVFLAHGEGDTVVPLAAARLARDWLRAEGHDVAWYAYPMTHEIIGAEIADLNAWLGERLVT

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=224, Percent_Identity=37.0535714285714, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI9966764, Length=220, Percent_Identity=36.8181818181818, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI20270341, Length=213, Percent_Identity=29.5774647887324, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17508057, Length=211, Percent_Identity=36.9668246445498, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323147, Length=211, Percent_Identity=33.6492890995261, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45553055, Length=206, Percent_Identity=37.378640776699, Blast_Score=133, Evalue=9e-32,
Organism=Drosophila melanogaster, GI45553057, Length=206, Percent_Identity=37.378640776699, Blast_Score=133, Evalue=9e-32,
Organism=Drosophila melanogaster, GI21357257, Length=206, Percent_Identity=37.378640776699, Blast_Score=133, Evalue=9e-32,
Organism=Drosophila melanogaster, GI161078179, Length=227, Percent_Identity=31.7180616740088, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 23777; Mature: 23777

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLKALEVETGRNPVASIILIHGLGASGRDLVSIAQALDLRSIGAVRFIFPNAPVRPVS
CCCCCCEEECCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHH
VCGGERMPAWYDLLAPDLLLREDEPGLRDAQTYLASLIDQEVARGIPSRRIVIGGFSQGC
HCCCCCCCHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
AMSLMTGLRYSSPLAGIAGLSGYLPLPGQTGREATEANRATPVFLAHGEGDTVVPLAAAR
HHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCEEHHHHHHH
LARDWLRAEGHDVAWYAYPMTHEIIGAEIADLNAWLGERLVT
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPLKALEVETGRNPVASIILIHGLGASGRDLVSIAQALDLRSIGAVRFIFPNAPVRPVS
CCCCCCEEECCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHH
VCGGERMPAWYDLLAPDLLLREDEPGLRDAQTYLASLIDQEVARGIPSRRIVIGGFSQGC
HCCCCCCCHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
AMSLMTGLRYSSPLAGIAGLSGYLPLPGQTGREATEANRATPVFLAHGEGDTVVPLAAAR
HHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEECCCCEEHHHHHHH
LARDWLRAEGHDVAWYAYPMTHEIIGAEIADLNAWLGERLVT
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA