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The map label for this gene is smeIM [H]

Identifier: 162145944

GI number: 162145944

Start: 115586

End: 116329

Strand: Direct

Name: smeIM [H]

Synonym: GDI_0108

Alternate gene names: 162145944

Gene position: 115586-116329 (Clockwise)

Preceding gene: 162145943

Following gene: 162145945

Centisome position: 2.93

GC content: 65.99

Gene sequence:

>744_bases
ATGACCCTCCTGACCGGCGACAGCGCGCTCCTGATGCCCTGGCACGGCCCCTACGACATGATCCTGGTCGATCCCCCCTA
TGGCGATACGTCGCTCGCATGGGACCGATGCGTGCCGGACTGGCCGGGCAAGGCGCTCGCCGCCCTGAAACCGAGTGGCT
CGCTCTGGGTGTTCGGTTCACTGCGGAGCTTTCTTGCATCCAGCGCCGCATTCCGGAATGCGGGCTGGAAATATGCCCAG
GAACTGGTCTGGGAGAAGCAGAACGGTTCCAGCTTCCATGCCGACCGTTTCCGCCGCGTGCATGAATTGCTCGTCCAGTT
CTATCGCGACGATACGCCCTGGCGTGCCGTTTACAACTCGGTCCCGACCACGCCCGACGCTCGGGCGCGCACGGTCCGGC
GGAAACACCGGCCGCCGCATATGGGGCAGATCGATGCCGGCCATTACGTCAGCGAGGATGGCGGCCCGCGCCTGATGCGT
TCCGTGATTCCGGTCCGCAATGCGCATGGCCGCGCCATCCATCCCACCGAGAAGCCGGTGGCCCTGCTGGAGATCCTGAT
CCGTACGAGCTGCCCGCCGGGCGGTCTGGTGGGTGACTGGTTCGCCGGATCGGGTGCGGCCGGTGTCGCCTGCCGATTGG
CTGGCCGGCGCTATGTCGGCTGCGAGATCGATCCTGTCATGGCGCAGAAGGCGCGTGACCGTATCGCGTCCGTGCTGCCG
CTCGGTGAAGGAATTTCGCCATGA

Upstream 100 bases:

>100_bases
ACGCCGCGCGCACCGGCCGCCTGGTCTGCGACCTGCGCCCGCACGGGATCGCAGCCCGCGAAATCGCCGCCCTGACGGCC
GAGATCGGGGGACTGGTGCC

Downstream 100 bases:

>100_bases
CGGAACGTCGCACGCCGCCCGGTTTCGCCGCGCGTCCCGCCGATCCTGAGCGCTGGGTCAGGGCGCCGGAGAAGCCTGCG
ACCCCCAACCGTTTTACCGC

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTLLTGDSALLMPWHGPYDMILVDPPYGDTSLAWDRCVPDWPGKALAALKPSGSLWVFGSLRSFLASSAAFRNAGWKYAQ
ELVWEKQNGSSFHADRFRRVHELLVQFYRDDTPWRAVYNSVPTTPDARARTVRRKHRPPHMGQIDAGHYVSEDGGPRLMR
SVIPVRNAHGRAIHPTEKPVALLEILIRTSCPPGGLVGDWFAGSGAAGVACRLAGRRYVGCEIDPVMAQKARDRIASVLP
LGEGISP

Sequences:

>Translated_247_residues
MTLLTGDSALLMPWHGPYDMILVDPPYGDTSLAWDRCVPDWPGKALAALKPSGSLWVFGSLRSFLASSAAFRNAGWKYAQ
ELVWEKQNGSSFHADRFRRVHELLVQFYRDDTPWRAVYNSVPTTPDARARTVRRKHRPPHMGQIDAGHYVSEDGGPRLMR
SVIPVRNAHGRAIHPTEKPVALLEILIRTSCPPGGLVGDWFAGSGAAGVACRLAGRRYVGCEIDPVMAQKARDRIASVLP
LGEGISP
>Mature_246_residues
TLLTGDSALLMPWHGPYDMILVDPPYGDTSLAWDRCVPDWPGKALAALKPSGSLWVFGSLRSFLASSAAFRNAGWKYAQE
LVWEKQNGSSFHADRFRRVHELLVQFYRDDTPWRAVYNSVPTTPDARARTVRRKHRPPHMGQIDAGHYVSEDGGPRLMRS
VIPVRNAHGRAIHPTEKPVALLEILIRTSCPPGGLVGDWFAGSGAAGVACRLAGRRYVGCEIDPVMAQKARDRIASVLPL
GEGISP

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 27205; Mature: 27074

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLTGDSALLMPWHGPYDMILVDPPYGDTSLAWDRCVPDWPGKALAALKPSGSLWVFGS
CEEEECCCEEEEECCCCEEEEEEECCCCCCCCCHHHCCCCCCCCEEEEECCCCCEEEHHH
LRSFLASSAAFRNAGWKYAQELVWEKQNGSSFHADRFRRVHELLVQFYRDDTPWRAVYNS
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHC
VPTTPDARARTVRRKHRPPHMGQIDAGHYVSEDGGPRLMRSVIPVRNAHGRAIHPTEKPV
CCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCCCEECCCCCHH
ALLEILIRTSCPPGGLVGDWFAGSGAAGVACRLAGRRYVGCEIDPVMAQKARDRIASVLP
HHHHHHHHCCCCCCCCEECCCCCCCCCCHHEEECCCEEECCCCCHHHHHHHHHHHHHHHC
LGEGISP
CCCCCCC
>Mature Secondary Structure 
TLLTGDSALLMPWHGPYDMILVDPPYGDTSLAWDRCVPDWPGKALAALKPSGSLWVFGS
EEEECCCEEEEECCCCEEEEEEECCCCCCCCCHHHCCCCCCCCEEEEECCCCCEEEHHH
LRSFLASSAAFRNAGWKYAQELVWEKQNGSSFHADRFRRVHELLVQFYRDDTPWRAVYNS
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHC
VPTTPDARARTVRRKHRPPHMGQIDAGHYVSEDGGPRLMRSVIPVRNAHGRAIHPTEKPV
CCCCCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCCCEECCCCCHH
ALLEILIRTSCPPGGLVGDWFAGSGAAGVACRLAGRRYVGCEIDPVMAQKARDRIASVLP
HHHHHHHHCCCCCCCCEECCCCCCCCCCHHEEECCCEEECCCCCHHHHHHHHHHHHHHHC
LGEGISP
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]