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The map label for this gene is rlmH [H]

Identifier: 229597122

GI number: 229597122

Start: 179298

End: 179768

Strand: Direct

Name: rlmH [H]

Synonym: NMCC_0177

Alternate gene names: 229597122

Gene position: 179298-179768 (Clockwise)

Preceding gene: 161869179

Following gene: 161869181

Centisome position: 8.33

GC content: 59.87

Gene sequence:

>471_bases
TTGAATATTACCGTTTTGGCAGTCGGCACCAAAATGCCGCGCTGGGTTGATGAGGCCGTCGCCGAATACGCCAAACGCTT
CGGACGCGACGTCGCCTACGCACTCAAAGAAATCAAACCCGAAAAACGCGGCGCGGGCGTGAATGCCGCCCAAGGTATGG
CGGCGGAAGAAAAACGCATCCTCGAAGCCATACCGCAAGGCGCGTTCCTCGTCGTCCTTGACGAACGCGGCAAAGCACCG
ACCTCCGTCGAGCTGGCGGAACACCTCAAAACTTGGCAGCAAAACGGCGAACACGTCTGCTTCGTCATCGGCGGCGCGGA
CGGAATGACCGACCGCCTCAAACAACAAGCCCGCATAATGATGCGCCTGTCCAGCCTGACCCTGCCGCACGGGATGGTGC
GCGTCCTTCTGACCGAGCAGCTCTACCGCGCCGCCTCCATCCTGCACAACCATCCCTATCATCGGGAATAA

Upstream 100 bases:

>100_bases
CGAGTTTCCACGCCGGAATGCAGAAGCCGTGGCACGCTGCAGACTGATTCCCGATGCCGTCTGAATGTTCAGACGGCATT
TTCCTGTAAGGGAGAAAGCA

Downstream 100 bases:

>100_bases
GAAGGCTTTTGTCCGTATCCCTAATCGGTTAAAATTACCCCGTTATTTCTGAACACAGCAAAGGAATCCGCTATGGCACG
CATGGTATTCTGCGTCAAGC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRGAGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAP
TSVELAEHLKTWQQNGEHVCFVIGGADGMTDRLKQQARIMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE

Sequences:

>Translated_156_residues
MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRGAGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAP
TSVELAEHLKTWQQNGEHVCFVIGGADGMTDRLKQQARIMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE
>Mature_156_residues
MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRGAGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAP
TSVELAEHLKTWQQNGEHVCFVIGGADGMTDRLKQQARIMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=42.3076923076923, Blast_Score=123, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 17416; Mature: 17416

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRGAGVNAAQGMAAEEKRI
CCEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCHHCCCCCCHHHCCHHHHHHH
LEAIPQGAFLVVLDERGKAPTSVELAEHLKTWQQNGEHVCFVIGGADGMTDRLKQQARIM
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
MRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRGAGVNAAQGMAAEEKRI
CCEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCHHCCCCCCHHHCCHHHHHHH
LEAIPQGAFLVVLDERGKAPTSVELAEHLKTWQQNGEHVCFVIGGADGMTDRLKQQARIM
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHH
MRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA