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The map label for this gene is mtrE [H]

Identifier: 161870560

GI number: 161870560

Start: 1654007

End: 1655410

Strand: Reverse

Name: mtrE [H]

Synonym: NMCC_1626

Alternate gene names: 161870560

Gene position: 1655410-1654007 (Counterclockwise)

Preceding gene: 161870561

Following gene: 161870557

Centisome position: 76.87

GC content: 56.2

Gene sequence:

>1404_bases
ATGGATACTACATTGAAAACCACCTTGACTTCTGTTGCAGCAGCCTTCGCATTATCCGCCTGCACCATGATTCCCCAATA
CGAGCAGCCCAAAGTCGAAGTTGCCGAAACGTTCAAAAACGATACCGCCGACAGCGGCATCCGTGCGGTCGATTTAGGTT
GGCATGACTATTTTGCCGACCCGCGCCTGCAAAAGCTGATCGACATCGCACTCGAGCGCAATACCAGTTTGCGTACCGCC
GTATTGAACAGCGAAATCTACCGCAAACAATACATGATTGAGCGCAACAACCTCCTGCCCACGCTTGCCGCCAATGCGAA
CGACTCGCGCCAAGGCAGCTTGAGCGGCGGCAATGTAAGCAGCAGCTACAAAGTCGGACTGGGTGCGGCATCTTACGAAC
TCGATTTGTTCGGGCGTGTACGCAGCAGCAGCGAGGCGGCACTGCAAGGCTATTTCGCCAGCACCGCCAACCGCGATGCG
GCACATTTGAGCCTGATTGCCACCGTTGCCAAAGCCTATTTCAACGAACGTTACGCCGAAGAAGCGATGTCTTTGGCGCA
ACGTGTTTTGAAAACGCGCGAGGAAACCTACAAGCTGTCCGAATTACGTTACAAGGCAGGCGTGATTTCCGCCGTCGCCC
TACGTCAGCAGGAAGCCCTAATCGAATCTGCCAAAGCCGATTATGCCCATGCCGCGCGCAGCCGCGAACAGGCGCGCAAT
GCCTTGGCAACCTTGATTAACCAACCGATACCCGAAGACCTGCCTGCCGGTTTGCCGCTGGACAAGCAGTTTTTTGTTGA
AAAACTGCCGGCCGGTTTGAGTTCCGAAGTATTGCTCGACCGTCCCGATATCCGTGCTGCCGAACACGCGCTCAAACAGG
CAAACGCCAATATCGGTGCGGCACGCGCCGCCTTTTTCCCATCCATCCGCCTGACCGGAACCGTCGGTACGGGTTCTGCC
GAATTGGGTGGGTTGTTCAAAAGCGGCACGGGCGTTTGGTCGTTCGCGCCGTCTATTACCCTGCCGATTTTTACCTGGGG
TACGAACAAGGCGAACCTTGATGTAGCCAAGCTGCGCCAACAGGCACAAATCGTTGCCTATGAATCCGCCGTCCAATCCG
CATTTCAAGACGTGGCAAACGCATTGGCGGCGCGCGAGCAGTTGGATAAAGCCTATGACGCTTTAAGCAAACAAAGCCGC
GCCTCTAAAGAAGCGTTGCGCTTGGTCGGCCTGCGTTACAAGCACGGCGTATCCGGCGCGCTCGACTTGCTCGATGCGGA
ACGCAGCAGCTATGCGGCGGAGGGTGCGGCTTTGTCGGCACAACTGACCCGCGCCGAAAACCTTGCCGATTTGTACAAGG
CACTCGGCGGCGGATTGAAACGGGATACCCAAACCGACAAATAA

Upstream 100 bases:

>100_bases
CGCCGCCGAAGCGGGCATCACCGGTTCGGACGACAGCCAACATTAAGCAACCATGACGTCTGAACGCCTACGGGTTTTCA
GACGGCATCAGGACTTTTTT

Downstream 100 bases:

>100_bases
CCGGTCGGGCAATAAAATGCCGTCTGAAACGATAAGGTTTCAGACGGCATTTCTTTCTTTATAGTGGATTAACAAAAACC
AGTACAGCGTTGGCTCGCCT

Product: outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTADSGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTA
VLNSEIYRKQYMIERNNLLPTLAANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEALIESAKADYAHAARSREQARN
ALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSEVLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSA
ELGGLFKSGTGVWSFAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAYDALSKQSR
ASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLADLYKALGGGLKRDTQTDK

Sequences:

>Translated_467_residues
MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTADSGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTA
VLNSEIYRKQYMIERNNLLPTLAANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEALIESAKADYAHAARSREQARN
ALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSEVLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSA
ELGGLFKSGTGVWSFAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAYDALSKQSR
ASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLADLYKALGGGLKRDTQTDK
>Mature_467_residues
MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTADSGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTA
VLNSEIYRKQYMIERNNLLPTLAANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEALIESAKADYAHAARSREQARN
ALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSEVLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSA
ELGGLFKSGTGVWSFAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAYDALSKQSR
ASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLADLYKALGGGLKRDTQTDK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=458, Percent_Identity=38.646288209607, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI2367348, Length=424, Percent_Identity=25.4716981132075, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50449; Mature: 50449

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTADSGIRAVDLGWHDYFAD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCHHHHCC
PRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTLAANANDSRQGSLSGGNVS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCC
SSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDAAHLSLIATVAKAYFNERYAE
CCEEEECCCCCEEHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEALIESAKADYAHAARSREQARN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSEVLLDRPDIRAAEHALKQANANIGA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCH
ARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWSFAPSITLPIFTWGTNKANLDVAKLRQ
HHHHHCCCEEEEEECCCCCHHHCCHHHCCCCCEEECCCCEEEEEEECCCCCCCHHHHHHH
QAQIVAYESAVQSAFQDVANALAAREQLDKAYDALSKQSRASKEALRLVGLRYKHGVSGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LDLLDAERSSYAAEGAALSAQLTRAENLADLYKALGGGLKRDTQTDK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTADSGIRAVDLGWHDYFAD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCHHHHCC
PRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTLAANANDSRQGSLSGGNVS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCC
SSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDAAHLSLIATVAKAYFNERYAE
CCEEEECCCCCEEHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEALIESAKADYAHAARSREQARN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSEVLLDRPDIRAAEHALKQANANIGA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCH
ARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWSFAPSITLPIFTWGTNKANLDVAKLRQ
HHHHHCCCEEEEEECCCCCHHHCCHHHCCCCCEEECCCCEEEEEEECCCCCCCHHHHHHH
QAQIVAYESAVQSAFQDVANALAAREQLDKAYDALSKQSRASKEALRLVGLRYKHGVSGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LDLLDAERSSYAAEGAALSAQLTRAENLADLYKALGGGLKRDTQTDK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]