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The map label for this gene is tesA [H]

Identifier: 161870521

GI number: 161870521

Start: 1614345

End: 1614932

Strand: Direct

Name: tesA [H]

Synonym: NMCC_1585

Alternate gene names: 161870521

Gene position: 1614345-1614932 (Clockwise)

Preceding gene: 161870520

Following gene: 161870522

Centisome position: 74.97

GC content: 55.44

Gene sequence:

>588_bases
TTGCTCCTTACCGCCTGCGGCAGAAAATCCGCCCGAACCCACGCCAAAATTCCCGAAGGAAGCACCGTACTTGCCTTGGG
CGATTCGCTTACCTTCGGCTACGGCGCAAACCCCGGCGAATCCTACCCCGCGCAACTGCAAAAACTGACGGGTTGGAATA
TTGTCAACGGCGGCGTATCGGGCGATACATCCGCCCAAGCCCTGTCGCGCCTGCCCGCGCTGTTGGCACGCAAACCCAAG
CTTGTGATTGTCGGCATAGGCGGCAACGACTTTCTGCGCAAAGTTCCCAAGGAGCAGACCCGCGCCAATATCGCGAAAAT
CATCGAAACCGTGCAGAAGGAAAACATCCCCGCCGTCCTCGTCGGCGTGCCGCACATCACACTGGGTGCGTTGTTCGGGC
ATTTGAGCGATCATCCGCTGTATGAGGATTTGTCCGAGGAATACGGCATTCCGCTGTTCGGCGGCGCGTGGGCGGAAATT
TTGGGCGATAATAATCTGAAATCCGACCAAATCCACGCCAACGGCAAAGGCTATCGGAAATTTGCCGAAGATTTGAATCA
ATTTTTGAGAAAACAGGGGTTTAGATAA

Upstream 100 bases:

>100_bases
TGTCCGGTTTACCGTGAAACCCTGAAATACCACCCGAAATGGCTCGATGCCATCTGAAAAACCAATGAACAGAAGAACCT
TCCTCCTCGGCGCAGGCGCG

Downstream 100 bases:

>100_bases
ACAAAGGTTTATCCGCACCCAAGTTGTTTATATATAGTGGATTAACAAAAATCAGGACAAGGCGACGAAGCCGCAAACAG
TACAAATAGTACGGCAAGGC

Product: lipase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MLLTACGRKSARTHAKIPEGSTVLALGDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPK
LVIVGIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYEDLSEEYGIPLFGGAWAEI
LGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR

Sequences:

>Translated_195_residues
MLLTACGRKSARTHAKIPEGSTVLALGDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPK
LVIVGIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYEDLSEEYGIPLFGGAWAEI
LGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR
>Mature_195_residues
MLLTACGRKSARTHAKIPEGSTVLALGDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPK
LVIVGIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYEDLSEEYGIPLFGGAWAEI
LGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=164, Percent_Identity=35.3658536585366, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 21015; Mature: 21015

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTACGRKSARTHAKIPEGSTVLALGDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVS
CEECCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHCCCEEEECCCC
GDTSAQALSRLPALLARKPKLVIVGIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVL
CCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEE
VGVPHITLGALFGHLSDHPLYEDLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRK
EECCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEECCCCHHHH
FAEDLNQFLRKQGFR
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MLLTACGRKSARTHAKIPEGSTVLALGDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVS
CEECCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHHCCCEEEECCCC
GDTSAQALSRLPALLARKPKLVIVGIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVL
CCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEE
VGVPHITLGALFGHLSDHPLYEDLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRK
EECCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEECCCCHHHH
FAEDLNQFLRKQGFR
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]