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The map label for this gene is eptA [H]

Identifier: 161870482

GI number: 161870482

Start: 1566314

End: 1567948

Strand: Reverse

Name: eptA [H]

Synonym: NMCC_1538

Alternate gene names: 161870482

Gene position: 1567948-1566314 (Counterclockwise)

Preceding gene: 161870483

Following gene: 161870481

Centisome position: 72.81

GC content: 52.05

Gene sequence:

>1635_bases
ATGATAAAACCGAACCTGAGGCCGAAGCTCGGCTCTTCCGCGCTGATTGCCTTCCTTTCCCTGTATTCCTCGCTGGTATT
GAATTACGCCTTTTTTGCCAAAGTTGTCGAGCTTCATCCTTTTAACGGCACCGGGGCGGATATCTTCCTCTATACGATGC
CGGTGGTGCTGTTTTTTTTAAGTAATTTCGTTTTTCACGTCATTGCCCTGCCTTTCGTGCATAAGGTATTGATTCCGTTA
ATATTGGTTATCAGTGCGGCGGTGTCTTACCAAGAAATATTTTTCAATATCTATTTCAACAAGTCGATGTTGAATAATGT
CTTGCAAACTACGGCTGCCGAAAGCGCGCGCCTGATTACGCCGGGCTATGTGCTGTGGATTGTATGTTTGGGCGTATTGC
CCGCGCTGGCGTATATCGCCGTCAAGGTTAAATACCGCGTTTGGTATAAGGAGCTTTTGACGCGCCTTGTGCTTGCCGCC
GTTTCCTTTTTGTGCGCGTTGGGCATCGCAATGTTGCAATATCAGGATTACGCCTCGTTTTTCCGCAACAATAAATCAGT
AACCCATCTGATTGTGCCGTCTAATTTCATCGGCGCGGGCGTGTCGAAATACAAAGATTGGAAGCGTTCCAATATTCCTT
ATACGCAATTGGATATGGCGGTCGTGCAAAACCGCCCGGCCGGCAGCCTGCGCCGTTTCGTGGTGCTGGTCGTGGGCGAG
ACCACGCGTGCCGCCAACTGGGGTTTGAACGGTTACAGCCGCCAAACTACGCCGCTGCTTGCCGCGCGCGGCGATGAAAT
TGTCAATTTCCCGCAGGTCAGAAGCTGCGGCACATCGACCGCGCACTCCCTGCCGTGTATGTTCTCAACCTTCGACCGCA
CGGATTATGACGAAATCAAAGCCGAACACCAAGACAACCTGCTGGACATCGTGCAGCGCGCCGGCGTGGAAGTTACCTGG
TTGGAAAACGATTCCGGCTGCAAGGGCGTGTGCGGCAAAGTGCCGAATACCGACGTTACCTCGCTCAACCTGCCCGAATA
CTGCCGCAACGGCGAGTGCCTCGACAATATCCTGCTGACTAAGTTCGACGAAGCCCTCAACAAAAACGATAAAGACGCGG
TTTTAATCCTGCATACCATCGGCAGTCACGGACCGACGTATTACGAACGCTACACCGAAGCCGAACGCAAATTCACGCCG
ACCTGCGACACCAATGAAATCGACAAATGCGCCCGCGCCACGCTGATCAACACTTACGACAATACGGTTTTGTATGTGGA
CCAGTTTATCGACAAGGTTATCCGCAAACTTGAAAACCGCGACGATTTGGAAAGCGTGGTGCATTATGTTTCCGACCACG
GCGAAAGTTTGGGCGAAAACGGGATGTACCTGCACGCCGCGCCTTACGCCATCGCGCCTTCCGGGCAGACGCATATCCCG
ATGGTTATGTGGTTTTCCAAAGCCTTCCGCCAACACGGGGGCATAGATTTCCAATGCCTCAAACAAAAAGCGGCGGAAAA
CGAATATTCGCACGACCACTATTTCAGCACGGTATTGGGGCTGATGGACATTTCCAACAGTCAAACCTATCGGAAGGAAA
TGGATATATTGGCAGCCTGCCGCCGTCCGCGCTGA

Upstream 100 bases:

>100_bases
GCCCTGCTTTGCTCCGTTGCCTTATAATTAAAAATCTTTTTCAATAATCCGGATTCCAAATGCCGGATGCCTTTTCCAAC
CCTTATCCGACACATTCCAA

Downstream 100 bases:

>100_bases
TGCCGGATATGCCGTCTGAAGCCTTCGGACGGCATATGTGTCCGAACACCCGACCGATGACCGGAGTATAACGATATGGA
TGCTTTAAAATTATTGACGA

Product: YhbX/YhjW/YijP/YjdB family protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGTGADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPL
ILVISAAVSYQEIFFNIYFNKSMLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLDMAVVQNRPAGSLRRFVVLVVGE
TTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSCGTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTW
LENDSGCKGVCGKVPNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYTEAERKFTP
TCDTNEIDKCARATLINTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYVSDHGESLGENGMYLHAAPYAIAPSGQTHIP
MVMWFSKAFRQHGGIDFQCLKQKAAENEYSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR

Sequences:

>Translated_544_residues
MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGTGADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPL
ILVISAAVSYQEIFFNIYFNKSMLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLDMAVVQNRPAGSLRRFVVLVVGE
TTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSCGTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTW
LENDSGCKGVCGKVPNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYTEAERKFTP
TCDTNEIDKCARATLINTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYVSDHGESLGENGMYLHAAPYAIAPSGQTHIP
MVMWFSKAFRQHGGIDFQCLKQKAAENEYSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR
>Mature_544_residues
MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGTGADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPL
ILVISAAVSYQEIFFNIYFNKSMLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLDMAVVQNRPAGSLRRFVVLVVGE
TTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSCGTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTW
LENDSGCKGVCGKVPNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYTEAERKFTP
TCDTNEIDKCARATLINTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYVSDHGESLGENGMYLHAAPYAIAPSGQTHIP
MVMWFSKAFRQHGGIDFQCLKQKAAENEYSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=540, Percent_Identity=43.8888888888889, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI87082286, Length=475, Percent_Identity=26.3157894736842, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI87082223, Length=458, Percent_Identity=27.292576419214, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790392, Length=496, Percent_Identity=24.7983870967742, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61320; Mature: 61320

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGTGADIFLYTMPVVLFFL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
SNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKSMLNNVLQTTAAESARLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCEECC
PGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAAVSFLCALGIAMLQYQDYASF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLDMAVVQNRPAGSLRRFVVLVVGE
HHCCCCEEEEEECCCHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHEEEEEEEECC
TTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSCGTSTAHSLPCMFSTFDRTDYDEIK
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHCCCCCHHCCCHHHHHCCCCCHHHHH
AEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKVPNTDVTSLNLPEYCRNGECLDNILLT
HHHCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
KFDEALNKNDKDAVLILHTIGSHGPTYYERYTEAERKFTPTCDTNEIDKCARATLINTYD
HHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCC
NTVLYVDQFIDKVIRKLENRDDLESVVHYVSDHGESLGENGMYLHAAPYAIAPSGQTHIP
CEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCCCCH
MVMWFSKAFRQHGGIDFQCLKQKAAENEYSHDHYFSTVLGLMDISNSQTYRKEMDILAAC
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
RRPR
CCCC
>Mature Secondary Structure
MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGTGADIFLYTMPVVLFFL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
SNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKSMLNNVLQTTAAESARLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHCCCEECC
PGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAAVSFLCALGIAMLQYQDYASF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLDMAVVQNRPAGSLRRFVVLVVGE
HHCCCCEEEEEECCCHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHEEEEEEEECC
TTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSCGTSTAHSLPCMFSTFDRTDYDEIK
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHCCCCCHHCCCHHHHHCCCCCHHHHH
AEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKVPNTDVTSLNLPEYCRNGECLDNILLT
HHHCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHH
KFDEALNKNDKDAVLILHTIGSHGPTYYERYTEAERKFTPTCDTNEIDKCARATLINTYD
HHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCC
NTVLYVDQFIDKVIRKLENRDDLESVVHYVSDHGESLGENGMYLHAAPYAIAPSGQTHIP
CEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCCCCCCCH
MVMWFSKAFRQHGGIDFQCLKQKAAENEYSHDHYFSTVLGLMDISNSQTYRKEMDILAAC
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
RRPR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]