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The map label for this gene is metZ [H]

Identifier: 161870460

GI number: 161870460

Start: 1541768

End: 1542937

Strand: Reverse

Name: metZ [H]

Synonym: NMCC_1515

Alternate gene names: 161870460

Gene position: 1542937-1541768 (Counterclockwise)

Preceding gene: 161870461

Following gene: 161870459

Centisome position: 71.65

GC content: 57.09

Gene sequence:

>1170_bases
ATGAGCAAAAAACTCCATCCGCAAACGCTCGCCATACGCGGCGGCAAAGAACAAACCGAATACCGCGAACACAATCAGGC
ATTGTTCCTGACCAGCAGCTTCATGTGGGACAACGCCCAACACGCTGCCGATTTGTTTTCAAAAAAAATCAAAGGCTTCA
CTTATACCCGTACCGCCAACCCGACCACAGCCGCCTTTGAAAAACGCATCGCCGCTTTGGAAGGTGCGGAACGCGCGGTC
GCCACTTCGACCGGCATGTCCGCGATTCAGGCGGCGTTTTTCACCTTTTTGCAGGCGGGCGACCATGTGATTTCCAGCCG
CAGCCTGTTCGGCACGACCGTCGGCTTTATCAATAACATCGTTACCAAATTCGGCATCGAAGTGAGTCACGTGTCGCCGA
CTGATATAAACGAATGGAAAGCCGCCGTCAAAGCCAACACCAAACTGCTGTTTTTGGAAACGCCGTCCAATCCCTTGGGC
GAAGTGGCCGACTTGGAAGCCTTGGCGGAATTGGCGCACGGCATCGGCGCGCTCTTGGTGGTGGACAACAGCCTGCTGTC
GCCTGTCGGCTCTCAGCCTTTGAAACACGGCGCGGATATTTCCGTTTCCTCCGCGACCAAAGCCATAGACGGACACGGGC
GCGTGATGGGCGGCGTGTTGGCGGGTTCGGAAGAACTGATGGCGCAGGTCGCCGTTTACTGCAACTCTTGCGGGCTGGCA
ATGTCGCCGTTTAACGCATGGCAGTTGTTGAGCGGCGTGGAAACCCTGTCTCTGCGTATGGAAAAACAGTTCGACAACGC
CTTGAAAATCGCGCAATGGCTGCAAGCGCAGCCGCAGGTTCAAGCCGTGTATTACACCGGATTACCCGACCATCCTCAGG
CGGAGCTTATCCGCAAACAGCAAAACGGCGGCGGCATCGTCATCGGCTTTGAAGTCGCCGACCAAGCAGCCGCGTGGAAA
GTCGTGGACGGCGTCGAACTTTTCTCCCGTACCGCCAACCTCGGCGACGTGCGCTCGACCATCACCCATCCGTGGACAAC
CACGCACGGCAGGATGCAGCCCGAAGAAAAACTCGCCGCCAACATCCGCCCCGGATTGGTGCGCCTGTCGGTCGGTTTGG
AATATGTTGGCGATTTGATTGACGATTTGAAACAGGCACTCGCGCGCTGA

Upstream 100 bases:

>100_bases
CTAAGGCGAGGCAACGCTGTACCGGTTTTTGTTAATCCACTATAAATTAAACAACGGGCGCACGCTCCGTTGCCGAACGG
ATAAAACCGAGGAATCACCG

Downstream 100 bases:

>100_bases
TTGCGTGTTCAGACGGCATCGGCGGAAAAACACCGCAAATGCCGTCTGAAACCCTTATCTTATATGGCATACACGTCAAC
AATAGGAAAAAAATATGGAT

Product: O-succinylhomoserine sulfhydrolase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAADLFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAV
ATSTGMSAIQAAFFTFLQAGDHVISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGGVLAGSEELMAQVAVYCNSCGLA
MSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQAQPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWK
VVDGVELFSRTANLGDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR

Sequences:

>Translated_389_residues
MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAADLFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAV
ATSTGMSAIQAAFFTFLQAGDHVISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGGVLAGSEELMAQVAVYCNSCGLA
MSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQAQPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWK
VVDGVELFSRTANLGDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR
>Mature_388_residues
SKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAADLFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVA
TSTGMSAIQAAFFTFLQAGDHVISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLGE
VADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGGVLAGSEELMAQVAVYCNSCGLAM
SPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQAQPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKV
VDGVELFSRTANLGDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=324, Percent_Identity=36.4197530864198, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI299473759, Length=324, Percent_Identity=33.641975308642, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI25453487, Length=323, Percent_Identity=30.6501547987616, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1790375, Length=382, Percent_Identity=34.0314136125654, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1789383, Length=379, Percent_Identity=26.3852242744063, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71980708, Length=353, Percent_Identity=33.9943342776204, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17538055, Length=390, Percent_Identity=33.5897435897436, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17560056, Length=342, Percent_Identity=35.672514619883, Blast_Score=204, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=32.6388888888889, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6319307, Length=349, Percent_Identity=38.10888252149, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=26.8472906403941, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=284, Percent_Identity=28.169014084507, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=347, Percent_Identity=29.971181556196, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922554, Length=341, Percent_Identity=38.1231671554252, Blast_Score=238, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 41967; Mature: 41836

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAADLFSKKIKGFTYTRTAN
CCCCCCCCEEEEECCCHHHHHHHCCCEEEEEEHHCCCCHHHHHHHHHHHHCCCEEEECCC
PTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDHVISSRSLFGTTVGFINNI
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHH
VTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLGEVADLEALAELAHGIGALLV
HHHHCCEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
VDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGGVLAGSEELMAQVAVYCNSCGLA
ECCHHHCCCCCCCHHCCCCCEECHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHCCCCE
MSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQAQPQVQAVYYTGLPDHPQAELIRKQ
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
QNGGGIVIGFEVADQAAAWKVVDGVELFSRTANLGDVRSTITHPWTTTHGRMQPEEKLAA
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCHHHHHH
NIRPGLVRLSVGLEYVGDLIDDLKQALAR
CCCCCEEEEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAADLFSKKIKGFTYTRTAN
CCCCCCCEEEEECCCHHHHHHHCCCEEEEEEHHCCCCHHHHHHHHHHHHCCCEEEECCC
PTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDHVISSRSLFGTTVGFINNI
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHH
VTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLGEVADLEALAELAHGIGALLV
HHHHCCEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEE
VDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGGVLAGSEELMAQVAVYCNSCGLA
ECCHHHCCCCCCCHHCCCCCEECHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHCCCCE
MSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQAQPQVQAVYYTGLPDHPQAELIRKQ
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHH
QNGGGIVIGFEVADQAAAWKVVDGVELFSRTANLGDVRSTITHPWTTTHGRMQPEEKLAA
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCHHHHHH
NIRPGLVRLSVGLEYVGDLIDDLKQALAR
CCCCCEEEEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]