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The map label for this gene is ilvI [H]

Identifier: 161870432

GI number: 161870432

Start: 1507886

End: 1509613

Strand: Reverse

Name: ilvI [H]

Synonym: NMCC_1480

Alternate gene names: 161870432

Gene position: 1509613-1507886 (Counterclockwise)

Preceding gene: 161870442

Following gene: 161870431

Centisome position: 70.1

GC content: 52.08

Gene sequence:

>1728_bases
ATGCAGTTATCAGGCGCGCAAATCATAGTGCAGAGTCTCAAAGCCGAAGGTGTCGAGTACGTTTTCGGTTATCCCGGCGG
TGCGGTTATCGAAATCTACGATGCCCTTTTCCAACTCAATAAATTCAAGCACATTCTGACCCGTCACGAGCAGGCGGCAG
TACACGCGGCAGATGCGTATGCGCGCGTCAGCGGTAAGGTGGGCGTGGCATTGGTTACATCCGGCCCGGGCGTTACCAAT
GCACTGACCGGTATTGCTACTGCCTATACGGATTCGATTCCGATGGTGGTCATCAGCGGGCAGGTAGGCAATTCCCTGAT
TGGTACGGATGCGTTCCAAGAAGTTGATACGGTGGGTATTACCCGTCCGTGCGTCAAACACAATTTCCTGGTTACGGACA
TCAATGAGTTGGCGGAAACCATTAAAAAGGCGTTCCAAATTGCCGCAAGCGGCCGACCGGGGCCCGTGGTGGTTGATGTC
CCGAAAGATGTTACGCAGGCGATGGCGAAATTCAGCTATCCTCAGGAAGACATTTTTATCCGTTCGTATCAACCGGTTGT
TCAAGGGCATATCGGTCAGATTAAAAAGGCCGTGCAGATGTTGGCATCTGCCAAACGTCCGGTCGTCTATTTTGGCGGCG
GCGTGGTGTTGGGTAATGCTTCTGAAGAGCTGACCCGATTTGTCCGAATGACGGGTGCTCCGTGCACGGGTACGCTGATG
GGCTTGGGCGCTTATCCTTCCGGCGACCGCCAATTCCTCGGTATGCTCGGTATGCACGGTACTTACGAGGCAAACCTTGC
CATGCAGAATGCGGATGTCGTTCTTGCCGTAGGTGCGCGTTTTGACGACCGTGTGGTTTCCGTACCGTCCAAATTTTTCG
AGAAGGCGAAGAAGGTTATCCATATCGATGTCGATCCTTCCAGCATCGCCAAACGCGTGAAGGCGGACATTCCGATTGTC
GGCGACGTGAAAAACGTTTTGTCCGAGATGGTTGCGCTGTGGCAAAAACAAGAGTCCGTGCCGTCTGAAGATACTTTGGG
CAAATGGTGGAAAACCATAGAGGAATGGCGTTCCCGAGATTGCTTGTGGTTTGACAACGGCAGCGAAATTATCAAGCCCC
AATATGTGATTCAGAAGCTTGCCGAGATTACCGGCAATTCGGCAATCATCACATCGGATGTAGGGCAGCATCAAATGTTT
GCGGCTCAATATTATCCCTTCGAACGTCCGCGCCAATGGCTCAATTCCGGCGGTTTGGGTACGATGGGCGTGGGTCTGCC
TTATGCGATTGGTGCAAAACTTGCCGCCCCGGATCAAGACGTATTCTGTATTACCGGCGACGGCTCGATTCAGATGAACA
TCCAAGAGTTGTCCACCTGTTTCCAATATCGGATTCCGGTTAACGTCATTACGCTGAACAACGGTTATCTCGGTATGGTA
CGCCAGTGGCAGGAAATATATTACGGCGGCCGAGAGTCGGAAACTTATTTCGATTCTTTGCCCGATTTCGTCAAACTTGC
CGAGGCATACGGTCATATCGGCATCCGCGTGGACAAGAAGTCTGATGTGGAAGGTGCGTTGTTGGAAGCATTGAACCAAA
AAGACAGGCTGGTGTTTATCGACTTCCTGACCGACCAGAAACAGAATGTGATGCCCATGGTCGGCAACGGCAAAGGTTTG
GACGAAATGGTACTTCCGCCGCATATGCGTGCGGACGAAAAGGCGTAA

Upstream 100 bases:

>100_bases
ATGCGCGTACCCTAAATCGCTGCTTGGTGGAATTGCATTACAGGTGCTGTGGCAAAGCGGTTTGTCCCTATTGGTTTGAA
ACCGTATAAAAGAGGTCGTT

Downstream 100 bases:

>100_bases
GGAGAGGCAAATGCGACATATCTTATCTGTTCTGATTGAAAACGAATCAGGTGCGATGAGCCGCGTGGTCGGTTTGTTCT
CTGCACGCGATTACAATATC

Product: acetolactate synthase isozyme III large subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKHILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTN
ALTGIATAYTDSIPMVVISGQVGNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLGNASEELTRFVRMTGAPCTGTLM
GLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLAVGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIV
GDVKNVLSEMVALWQKQESVPSEDTLGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITSDVGQHQMF
AAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSIQMNIQELSTCFQYRIPVNVITLNNGYLGMV
RQWQEIYYGGRESETYFDSLPDFVKLAEAYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGL
DEMVLPPHMRADEKA

Sequences:

>Translated_575_residues
MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKHILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTN
ALTGIATAYTDSIPMVVISGQVGNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLGNASEELTRFVRMTGAPCTGTLM
GLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLAVGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIV
GDVKNVLSEMVALWQKQESVPSEDTLGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITSDVGQHQMF
AAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSIQMNIQELSTCFQYRIPVNVITLNNGYLGMV
RQWQEIYYGGRESETYFDSLPDFVKLAEAYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGL
DEMVLPPHMRADEKA
>Mature_575_residues
MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKHILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTN
ALTGIATAYTDSIPMVVISGQVGNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLGNASEELTRFVRMTGAPCTGTLM
GLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLAVGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIV
GDVKNVLSEMVALWQKQESVPSEDTLGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITSDVGQHQMF
AAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSIQMNIQELSTCFQYRIPVNVITLNNGYLGMV
RQWQEIYYGGRESETYFDSLPDFVKLAEAYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGL
DEMVLPPHMRADEKA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=27.4509803921569, Blast_Score=185, Evalue=9e-47,
Organism=Homo sapiens, GI21361361, Length=517, Percent_Identity=27.6595744680851, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=57.773851590106, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=571, Percent_Identity=40.8056042031524, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1786717, Length=532, Percent_Identity=33.4586466165414, Blast_Score=288, Evalue=7e-79,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=28.2882882882883, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788716, Length=563, Percent_Identity=26.2877442273535, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=572, Percent_Identity=29.3706293706294, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17531301, Length=572, Percent_Identity=29.5454545454545, Blast_Score=199, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542570, Length=547, Percent_Identity=28.3363802559415, Blast_Score=168, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=43.298969072165, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=517, Percent_Identity=26.4990328820116, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321524, Length=502, Percent_Identity=21.9123505976096, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=513, Percent_Identity=22.8070175438596, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=542, Percent_Identity=22.3247232472325, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=518, Percent_Identity=29.9227799227799, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62887; Mature: 62887

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKHILTRHEQAAVHAADAY
CCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQVGNSLIGTDAFQEVDTVGI
HHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCC
TRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDVPKDVTQAMAKFSYPQEDIFI
CCCHHHCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCHHEEE
RSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLGNASEELTRFVRMTGAPCTGTLM
ECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCCCCCEE
GLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLAVGARFDDRVVSVPSKFFEKAKKVI
ECCCCCCCCHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCCHHHHCCHHHHHHCCEEE
HIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALWQKQESVPSEDTLGKWWKTIEEWRSRD
EEECCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
CLWFDNGSEIIKPQYVIQKLAEITGNSAIITSDVGQHQMFAAQYYPFERPRQWLNSGGLG
CEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHCCCCCC
TMGVGLPYAIGAKLAAPDQDVFCITGDGSIQMNIQELSTCFQYRIPVNVITLNNGYLGMV
EECCCCCHHHCCEECCCCCCEEEEECCCCEEEEHHHHHHHHHEECCEEEEEECCCCHHHH
RQWQEIYYGGRESETYFDSLPDFVKLAEAYGHIGIRVDKKSDVEGALLEALNQKDRLVFI
HHHHHHHCCCCCCCHHHHCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCCCEEEE
DFLTDQKQNVMPMVGNGKGLDEMVLPPHMRADEKA
EEECCCCCCCCCEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKHILTRHEQAAVHAADAY
CCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQVGNSLIGTDAFQEVDTVGI
HHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCC
TRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDVPKDVTQAMAKFSYPQEDIFI
CCCHHHCCEEEECHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCHHEEE
RSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLGNASEELTRFVRMTGAPCTGTLM
ECCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCCCCCEE
GLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLAVGARFDDRVVSVPSKFFEKAKKVI
ECCCCCCCCHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCCHHHHCCHHHHHHCCEEE
HIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALWQKQESVPSEDTLGKWWKTIEEWRSRD
EEECCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
CLWFDNGSEIIKPQYVIQKLAEITGNSAIITSDVGQHQMFAAQYYPFERPRQWLNSGGLG
CEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHCCCCCC
TMGVGLPYAIGAKLAAPDQDVFCITGDGSIQMNIQELSTCFQYRIPVNVITLNNGYLGMV
EECCCCCHHHCCEECCCCCCEEEEECCCCEEEEHHHHHHHHHEECCEEEEEECCCCHHHH
RQWQEIYYGGRESETYFDSLPDFVKLAEAYGHIGIRVDKKSDVEGALLEALNQKDRLVFI
HHHHHHHCCCCCCCHHHHCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCCCEEEE
DFLTDQKQNVMPMVGNGKGLDEMVLPPHMRADEKA
EEECCCCCCCCCEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]